Details of the Target

General Information of Target

Target ID LDTP04426
Target Name B-cell receptor-associated protein 31 (BCAP31)
Gene Name BCAP31
Gene ID 10134
Synonyms
BAP31; DXS1357E; B-cell receptor-associated protein 31; BCR-associated protein 31; Bap31; 6C6-AG tumor-associated antigen; Protein CDM; p28
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSLQWTAVATFLYAEVFVVLLLCIPFISPKRWQKIFKSRLVELLVSYGNTFFVVLIVILV
LLVIDAVREIRKYDDVTEKVNLQNNPGAMEHFHMKLFRAQRNLYIAGFSLLLSFLLRRLV
TLISQQATLLASNEAFKKQAESASEAAKKYMEENDQLKKGAAVDGGKLDVGNAEVKLEEE
NRSLKADLQKLKDELASTKQKLEKAENQVLAMRKQSEGLTKEYDRLLEEHAKLQAAVDGP
MDKKEE
Target Bioclass
Transporter and channel
Family
BCAP29/BCAP31 family
Subcellular location
Endoplasmic reticulum membrane
Function
Functions as a chaperone protein. Is one of the most abundant endoplasmic reticulum (ER) proteins. Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). Also serves as a cargo receptor for the export of transmembrane proteins. Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by stimulating the translocation of NDUFS4 and NDUFB11 from the cytosol to the mitochondria via interaction with TOMM40. In response to ER stress, delocalizes from the ER-mitochondria contact sites and binds BCL2. May be involved in CASP8-mediated apoptosis.
Uniprot ID
P51572
Ensemble ID
ENST00000345046.12
HGNC ID
HGNC:16695
ChEMBL ID
CHEMBL4295778

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.V8I .
SNGM SNV: p.Y104H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 22 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HDSF-alk
 Probe Info  		2.02  LDD0197  [1]
P8
 Probe Info  		4.25  LDD0451  [2]
CHEMBL5175495
 Probe Info  		9.49  LDD0196  [3]
CY4
 Probe Info  		100.00  LDD0244  [4]
TG42
 Probe Info  		10.58  LDD0326  [5]
TH211
 Probe Info  		Y73(8.00)  LDD0260  [6]
C-Sul
 Probe Info  		6.24  LDD0066  [7]
YN-1
 Probe Info  		100.00  LDD0444  [8]
ONAyne
 Probe Info  		K138(1.04); K159(5.00)  LDD0274  [9]
OPA-S-S-alkyne
 Probe Info  		K176(0.75); K204(1.82)  LDD3494  [10]
Probe 1
 Probe Info  		Y150(59.44)  LDD3495  [11]
m-APA
 Probe Info  		11.35  LDD0403  [12]
Jackson_1
 Probe Info  		10.61  LDD0120  [13]
HPAP
 Probe Info  		3.78  LDD0064  [14]
ATP probe
 Probe Info  		K95(0.00); K204(0.00); K72(0.00); K214(0.00)  LDD0199  [15]
ATP probe
 Probe Info  		K95(0.00); K221(0.00); K192(0.00)  LDD0035  [16]
NHS
 Probe Info  		K201(0.00); K221(0.00); K176(0.00); K167(0.00)  LDD0010  [17]
SF
 Probe Info  		Y150(0.00); K192(0.00); K190(0.00)  LDD0028  [18]
STPyne
 Probe Info  		N.A.  LDD0009  [17]
1c-yne
 Probe Info  		K149(0.00); K167(0.00); K190(0.00)  LDD0228  [19]
Acrolein
 Probe Info  		H93(0.00); H230(0.00)  LDD0217  [20]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [20]
PAL-AfBPP Probe
Click To Hide/Show 46 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DR-1
 Probe Info  		2.36  LDD0398  [21]
IMP2070
 Probe Info  		2.99  LDD0280  [22]
C022
 Probe Info  		6.82  LDD1728  [23]
C040
 Probe Info  		9.06  LDD1740  [23]
C056
 Probe Info  		18.25  LDD1753  [23]
C063
 Probe Info  		10.34  LDD1760  [23]
C087
 Probe Info  		13.45  LDD1779  [23]
C091
 Probe Info  		10.78  LDD1782  [23]
C092
 Probe Info  		19.84  LDD1783  [23]
C094
 Probe Info  		37.79  LDD1785  [23]
C112
 Probe Info  		18.77  LDD1799  [23]
C143
 Probe Info  		13.18  LDD1825  [23]
C158
 Probe Info  		9.06  LDD1838  [23]
C165
 Probe Info  		12.73  LDD1845  [23]
C201
 Probe Info  		32.22  LDD1877  [23]
C218
 Probe Info  		10.70  LDD1892  [23]
C220
 Probe Info  		11.88  LDD1894  [23]
C228
 Probe Info  		15.03  LDD1901  [23]
C231
 Probe Info  		21.41  LDD1904  [23]
C232
 Probe Info  		37.27  LDD1905  [23]
C234
 Probe Info  		7.67  LDD1907  [23]
C235
 Probe Info  		24.42  LDD1908  [23]
C289
 Probe Info  		31.34  LDD1959  [23]
C299
 Probe Info  		6.41  LDD1968  [23]
C339
 Probe Info  		5.66  LDD2002  [23]
C348
 Probe Info  		17.15  LDD2009  [23]
C349
 Probe Info  		8.82  LDD2010  [23]
C350
 Probe Info  		25.81  LDD2011  [23]
C355
 Probe Info  		22.94  LDD2016  [23]
C362
 Probe Info  		53.82  LDD2023  [23]
C363
 Probe Info  		16.91  LDD2024  [23]
C388
 Probe Info  		36.25  LDD2047  [23]
C390
 Probe Info  		23.10  LDD2049  [23]
FFF probe11
 Probe Info  		20.00  LDD0471  [24]
FFF probe13
 Probe Info  		18.33  LDD0475  [24]
FFF probe14
 Probe Info  		20.00  LDD0477  [24]
FFF probe2
 Probe Info  		14.91  LDD0463  [24]
FFF probe3
 Probe Info  		17.20  LDD0464  [24]
STS-1
 Probe Info  		N.A.  LDD0136  [25]
STS-2
 Probe Info  		N.A.  LDD0138  [25]
VE-P
 Probe Info  		N.A.  LDD0396  [26]
AX-1
 Probe Info  		3.74  LDD0441  [27]
DFG-out-4
 Probe Info  		4.60  LDD0075  [28]
Staurosporine capture compound
 Probe Info  		2.00  LDD0083  [29]
KIRA6 AfBP
 Probe Info  		N.A.  LDD0378  [30]
OEA-DA
 Probe Info  		10.34  LDD0046  [31]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0176  9im MDA-MB-231 3.74  LDD0441  [27]
 LDCM0156  Aniline NCI-H1299 11.35  LDD0403  [12]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [20]
 LDCM0070  Cisar_cp37 MDA-MB-231 10.61  LDD0120  [13]
 LDCM0017  DFG-out-2 A431 4.60  LDD0075  [28]
 LDCM0107  IAA HeLa N.A.  LDD0221  [20]
 LDCM0109  NEM HeLa H93(0.00); H230(0.00)  LDD0223  [20]
 LDCM0014  Panhematin hPBMC 3.78  LDD0064  [14]
 LDCM0019  Staurosporine Hep-G2 2.00  LDD0083  [29]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cystic fibrosis transmembrane conductance regulator (CFTR) ABCC family P13569
Caspase-8 (CASP8) Peptidase C14A family Q14790
Kallikrein-6 (KLK6) Peptidase S1 family Q92876
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 (HACD2) Very long-chain fatty acids dehydratase HACD family Q6Y1H2
Transporter and channel
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067
Apoptosis regulator Bcl-2 (BCL2) Bcl-2 family P10415
Derlin-1 (DERL1) Derlin family Q9BUN8
Huntingtin (HTT) Huntingtin family P42858
Protein transport protein Sec61 subunit beta (SEC61B) SEC61-beta family P60468
Translocating chain-associated membrane protein 1 (TRAM1) TRAM family Q15629
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mitochondrial fission 1 protein (FIS1) FIS1 family Q9Y3D6
Complex I assembly factor TIMMDC1, mitochondrial (TIMMDC1) Tim17/Tim22/Tim23 family Q9NPL8

References

1 Fatty Acyl Sulfonyl Fluoride as an Activity-Based Probe for Profiling Fatty Acid-Associated Proteins in Living Cells. Chembiochem. 2022 Feb 16;23(4):e202100628. doi: 10.1002/cbic.202100628. Epub 2021 Dec 30.
2 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
3 Charting the Chemical Space of Acrylamide-Based Inhibitors of zDHHC20. ACS Med Chem Lett. 2022 Sep 26;13(10):1648-1654. doi: 10.1021/acsmedchemlett.2c00336. eCollection 2022 Oct 13.
4 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
5 Design and synthesis of tailored human caseinolytic protease P inhibitors. Chem Commun (Camb). 2018 Aug 28;54(70):9833-9836. doi: 10.1039/c8cc05265d.
Mass spectrometry data entry: PXD010277
6 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
7 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
8 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
9 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
10 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
11 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
12 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
13 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
14 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
15 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
16 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
17 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
18 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
19 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
20 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
21 Quantitative Proteomics Reveals Cellular Off-Targets of a DDR1 Inhibitor. ACS Med Chem Lett. 2020 Feb 5;11(4):535-540. doi: 10.1021/acsmedchemlett.9b00658. eCollection 2020 Apr 9.
22 A Probe for NLRP3 Inflammasome Inhibitor MCC950 Identifies Carbonic Anhydrase 2 as a Novel Target. ACS Chem Biol. 2021 Jun 18;16(6):982-990. doi: 10.1021/acschembio.1c00218. Epub 2021 May 18.
Mass spectrometry data entry: PXD024915 , PXD024913
23 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
24 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
25 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
26 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
27 Cell- and Tissue-Based Proteome Profiling and Bioimaging with Probes Derived from a Potent AXL Kinase Inhibitor. Chem Asian J. 2018 Sep 17;13(18):2601-2605. doi: 10.1002/asia.201800605. Epub 2018 Aug 7.
28 Affinity-based probes based on type II kinase inhibitors. J Am Chem Soc. 2012 Nov 21;134(46):19017-25. doi: 10.1021/ja306035v. Epub 2012 Nov 6.
29 Comprehensive identification of staurosporine-binding kinases in the hepatocyte cell line HepG2 using Capture Compound Mass Spectrometry (CCMS). J Proteome Res. 2010 Feb 5;9(2):806-17. doi: 10.1021/pr9007333.
30 Kinase Photoaffinity Labeling Reveals Low Selectivity Profile of the IRE1 Targeting Imidazopyrazine-Based KIRA6 Inhibitor. ACS Chem Biol. 2020 Dec 18;15(12):3106-3111. doi: 10.1021/acschembio.0c00802. Epub 2020 Dec 8.
Mass spectrometry data entry: PXD022628
31 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570