Probe Name	DFG-out-4					Probe Info
Probe concentration	1 uM
Criteria	Quantification: Probe vs (Probe+Competitor)
Quantitative Method	SILAC
Competitor Name	DFG-out-2					Competitor Info
Competitor Concentration	10 uM
In Vivo Experiment Model	Model Type			Cell lysate
	Derived Tissue			Skin
	Disease Model		Skin squamous cell carcinoma [ICD-11:2C31]
	Model Name		Human skin squamous cell carcinoma cell lysate (A431)

Target ID	Target Name					Binding Ratio
LDTP04631	Ephrin type-B receptor 4 (EPHB4)					71.0
LDTP05453	Focal adhesion kinase 1 (PTK2)					37.0
LDTP03477	Ephrin type-B receptor 2 (EPHB2)					35.0
LDTP11819	STE20-like serine/threonine-protein kinase (SLK)					27.0
LDTP01349	Short-chain dehydrogenase/reductase 3 (DHRS3)					20.3
LDTP04584	Mitogen-activated protein kinase 10 (MAPK10)					15.0
LDTP06353	Receptor tyrosine-protein kinase erbB-4 (ERBB4)					11.0
LDTP05917	Receptor-interacting serine/threonine-protein kinase 1 (RIPK1)					11.0
LDTP01420	Serine/threonine-protein kinase 10 (STK10)					11.0
LDTP06459	Myosin light chain kinase, smooth muscle (MYLK)					9.2
LDTP03944	Tyrosine-protein kinase CSK (CSK)					8.8
LDTP04075	Mitogen-activated protein kinase 8 (MAPK8)					6.3
LDTP01785	Epidermal growth factor receptor (EGFR)					6.1
LDTP00325	Pirin (PIR)					5.2
LDTP03474	Ephrin type-A receptor 2 (EPHA2)					5.0
LDTP06270	Signal peptidase complex subunit 2 (SPCS2)					4.8
LDTP03375	Hepatocyte growth factor-like protein (MST1)					4.6
LDTP11733	Ras-related protein Rab-1B (RAB1B)					4.3
LDTP11686	Ras-related protein Rab-33B (RAB33B)					4.3
LDTP12046	Ubiquitin-conjugating enzyme E2 Z (UBE2Z)					4.0
LDTP06100	Protein-tyrosine kinase 2-beta (PTK2B)					3.9
LDTP13031	Serine/threonine-protein kinase 26 (STK26)					3.8
LDTP13446	TRAF2 and NCK-interacting protein kinase (TNIK)					3.2
LDTP14240	Serine/threonine-protein kinase 24 (STK24)					2.4
LDTP05776	Serine/threonine-protein kinase PAK 2 (PAK2)					2.3
LDTP04629	Ephrin type-B receptor 3 (EPHB3)					0.0
LDTP01685	Mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4)					0.0
LDTP04440	Peroxisomal multifunctional enzyme type 2 (HSD17B4)					0.0
LDTP05996	Protein-tyrosine kinase 6 (PTK6)					0.0
LDTP02638	Proto-oncogene tyrosine-protein kinase Src (SRC)					0.0
LDTP00907	Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta (PDE6D)					0.0
LDTP03008	Spermidine synthase (SRM)					0.0
LDTP03992	Tyrosine-protein kinase FRK (FRK)					0.0
LDTP04048	Tyrosine-protein kinase SYK (SYK)					0.0

Target ID	Target Name					Binding Ratio
LDTP03546	Translocator protein (TSPO)					21.1
LDTP06944	Transmembrane protein 41B (TMEM41B)					6.4
LDTP10663	Importin-9 (IPO9)					5.1
LDTP06365	Transmembrane emp24 domain-containing protein 2 (TMED2)					4.9
LDTP04426	B-cell receptor-associated protein 31 (BCAP31)					4.6
LDTP08479	Transmembrane protein 139 (TMEM139)					0.0

Target ID	Target Name					Binding Ratio
LDTP03619	Stress-induced-phosphoprotein 1 (STIP1)					12.5
LDTP00759	Tumor protein D54 (TPD52L2)					7.9
LDTP01522	Reticulon-3 (RTN3)					7.1
LDTP01021	Perilipin-3 (PLIN3)					6.7
LDTP04703	Tumor protein D52 (TPD52)					6.3
LDTP00759	Tumor protein D54 (TPD52L2)					6.3
LDTP18533	Transgelin-3 (TAGLN3)					6.0
LDTP04500	Heterogeneous nuclear ribonucleoprotein M (HNRNPM)					5.0
LDTP07301	ADP-ribosylation factor-like protein 6-interacting protein 4 (ARL6IP4)					4.6

1	Affinity-based probes based on type II kinase inhibitors. J Am Chem Soc. 2012 Nov 21;134(46):19017-25. doi: 10.1021/ja306035v. Epub 2012 Nov 6.