Details of the Target

General Information of Target

Target ID LDTP11733
Target Name Ras-related protein Rab-1B (RAB1B)
Gene Name RAB1B
Gene ID 81876
Synonyms
Ras-related protein Rab-1B; EC 3.6.5.2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAARWRFWCVSVTMVVALLIVCDVPSASAQRKKEMVLSEKVSQLMEWTNKRPVIRMNGDK
FRRLVKAPPRNYSVIVMFTALQLHRQCVVCKQADEEFQILANSWRYSSAFTNRIFFAMVD
FDEGSDVFQMLNMNSAPTFINFPAKGKPKRGDTYELQVRGFSAEQIARWIADRTDVNIRV
IRPPNYAGPLMLGLLLAVIGGLVYLRRSNMEFLFNKTGWAFAALCFVLAMTSGQMWNHIR
GPPYAHKNPHTGHVNYIHGSSQAQFVAETHIVLLFNGGVTLGMVLLCEAATSDMDIGKRK
IMCVAGIGLVVLFFSWMLSIFRSKYHGYPYSFLMS
Target Bioclass
Enzyme
Family
Small GTPase superfamily, Rab family
Subcellular location
Cytoplasm
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Required to modulate the compacted morphology of the Golgi. Promotes the recruitment of lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment.
Uniprot ID
Q9H0U4
Ensemble ID
ENST00000311481.11
HGNC ID
HGNC:18370

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 20 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		10.21  LDD0402  [1]
A-EBA
 Probe Info  		5.91  LDD0215  [2]
C-Sul
 Probe Info  		3.02  LDD0066  [3]
YN-1
 Probe Info  		100.00  LDD0444  [4]
ONAyne
 Probe Info  		K128(10.00); K55(0.17)  LDD0274  [5]
STPyne
 Probe Info  		K122(8.12); K128(8.22); K129(7.33); K170(6.47)  LDD0277  [5]
AZ-9
 Probe Info  		E51(1.09)  LDD2208  [6]
AF-1
 Probe Info  		10.00  LDD0421  [7]
AF-2
 Probe Info  		10.00  LDD0422  [7]
HHS-475
 Probe Info  		Y109(1.03)  LDD0264  [8]
Acrolein
 Probe Info  		N.A.  LDD0227  [9]
IA-alkyne
 Probe Info  		N.A.  LDD0163  [10]
TFBX
 Probe Info  		N.A.  LDD0027  [11]
SF
 Probe Info  		Y78(0.00); Y109(0.00); Y77(0.00); Y7(0.00)  LDD0028  [12]
1c-yne
 Probe Info  		N.A.  LDD0228  [13]
AOyne
 Probe Info  		6.50  LDD0443  [14]
MPP-AC
 Probe Info  		N.A.  LDD0428  [15]
NAIA_5
 Probe Info  		N.A.  LDD2223  [16]
TER-AC
 Probe Info  		N.A.  LDD0426  [15]
HHS-465
 Probe Info  		N.A.  LDD2240  [17]
PAL-AfBPP Probe
Click To Hide/Show 21 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C040
 Probe Info  		8.69  LDD1740  [18]
C094
 Probe Info  		23.59  LDD1785  [18]
C112
 Probe Info  		17.03  LDD1799  [18]
C169
 Probe Info  		19.84  LDD1849  [18]
C232
 Probe Info  		35.75  LDD1905  [18]
C235
 Probe Info  		24.93  LDD1908  [18]
C264
 Probe Info  		17.27  LDD1935  [18]
C265
 Probe Info  		13.36  LDD1936  [18]
C299
 Probe Info  		5.98  LDD1968  [18]
C362
 Probe Info  		61.82  LDD2023  [18]
C364
 Probe Info  		16.68  LDD2025  [18]
C366
 Probe Info  		6.23  LDD2027  [18]
C390
 Probe Info  		23.75  LDD2049  [18]
FFF probe11
 Probe Info  		8.13  LDD0471  [19]
FFF probe13
 Probe Info  		15.95  LDD0475  [19]
FFF probe14
 Probe Info  		12.10  LDD0477  [19]
FFF probe2
 Probe Info  		6.75  LDD0463  [19]
FFF probe3
 Probe Info  		20.00  LDD0464  [19]
JN0003
 Probe Info  		9.65  LDD0469  [19]
DFG-out-4
 Probe Info  		4.30  LDD0075  [20]
OEA-DA
 Probe Info  		4.42  LDD0046  [21]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0166  Afatinib A431 10.00  LDD0421  [7]
 LDCM0156  Aniline NCI-H1299 11.42  LDD0403  [1]
 LDCM0017  DFG-out-2 A431 4.30  LDD0075  [20]
 LDCM0116  HHS-0101 DM93 Y109(1.03)  LDD0264  [8]
 LDCM0117  HHS-0201 DM93 Y109(1.06)  LDD0265  [8]
 LDCM0118  HHS-0301 DM93 Y109(0.98)  LDD0266  [8]
 LDCM0119  HHS-0401 DM93 Y109(1.25)  LDD0267  [8]
 LDCM0120  HHS-0701 DM93 Y109(0.82)  LDD0268  [8]
 LDCM0109  NEM HeLa N.A.  LDD0227  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Inositol polyphosphate 5-phosphatase OCRL (OCRL) Inositol 1,4,5-trisphosphate 5-phosphatase type II family Q01968
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2) TKL Ser/Thr protein kinase family Q5S007
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Guanine nucleotide exchange factor MSS4 (RABIF) DSS4/MSS4 family P47224

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
3 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
4 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
5 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
6 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
7 Minimalist linkers suitable for irreversible inhibitors in simultaneous proteome profiling, live-cell imaging and drug screening. Chem Commun (Camb). 2019 Jan 15;55(6):834-837. doi: 10.1039/c8cc08685k.
8 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
9 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
10 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
11 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
12 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
13 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
14 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
15 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
16 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
17 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
18 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
19 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
20 Affinity-based probes based on type II kinase inhibitors. J Am Chem Soc. 2012 Nov 21;134(46):19017-25. doi: 10.1021/ja306035v. Epub 2012 Nov 6.
21 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570