Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C367  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 79 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP11187  COP9 signalosome complex subunit 4 (COPS4) 46.5
 LDTP10699  E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) 34.8
 LDTP00907  Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta (PDE6D) 29.2
 LDTP17247  5'-nucleotidase domain-containing protein 1 (NT5DC1) 26.5
 LDTP00697  Cytochrome b5 type B (CYB5B) 21.1
 LDTP00831  NADH dehydrogenase 1 beta subcomplex subunit 5, mitochondrial (NDUFB5) 19.3
 LDTP19851  Isochorismatase domain-containing protein 1 (ISOC1) 18.8
 LDTP05066  Signal peptidase complex catalytic subunit SEC11A (SEC11A) 16.0
 LDTP04712  Puromycin-sensitive aminopeptidase (NPEPPS) 13.4
 LDTP04437  Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 13.3
 LDTP12253  Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM) 13.1
 LDTP03540  Heme oxygenase 2 (HMOX2) 12.6
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 11.8
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 11.0
 LDTP01335  Protein SCO1 homolog, mitochondrial (SCO1) 10.6
 LDTP11319  Threonine--tRNA ligase, mitochondrial (TARS2) 10.6
 LDTP01982  NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) 10.3
 LDTP12628  Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10) 10.2
 LDTP09096  Carbohydrate sulfotransferase 14 (CHST14) 9.6
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 9.4
 LDTP03787  Choline kinase alpha (CHKA) 9.4
 LDTP01039  Protein-S-isoprenylcysteine O-methyltransferase (ICMT) 9.4
 LDTP04051  Nicotinamide phosphoribosyltransferase (NAMPT) 8.5
 LDTP12197  Ethanolamine kinase 1 (ETNK1) 8.3
 LDTP06511  Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial (ETFDH) 8.3
 LDTP14218  Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) 8.2
 LDTP12873  CDGSH iron-sulfur domain-containing protein 1 (CISD1) 8.0
 LDTP10329  Ceramide synthase 2 (CERS2) 7.9
 LDTP13751  Exonuclease 1 (EXO1) 7.8
 LDTP12363  Lysophosphatidic acid phosphatase type 6 (ACP6) 7.7
 LDTP11127  ADP-dependent glucokinase (ADPGK) 7.6
 LDTP09799  DCN1-like protein 4 (DCUN1D4) 7.4
 LDTP06787  Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) 7.3
 LDTP04289  Very long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADVL) 7.3
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 7.2
 LDTP01773  Cytochrome c oxidase subunit 2 (MT-CO2) 7.0
 LDTP07620  Peroxisomal N(1)-acetyl-spermine/spermidine oxidase (PAOX) 7.0
 LDTP14276  NADH dehydrogenase 1 beta subcomplex subunit 9 (NDUFB9) 6.9
 LDTP04471  Ribosomal protein S6 kinase alpha-3 (RPS6KA3) 6.9
 LDTP03537  Glycylpeptide N-tetradecanoyltransferase 1 (NMT1) 6.8
 LDTP05476  3-ketodihydrosphingosine reductase (KDSR) 6.7
 LDTP08064  ATP-dependent RNA helicase DHX29 (DHX29) 6.6
 LDTP00783  Beta-1,4-glucuronyltransferase 1 (B4GAT1) 6.6
 LDTP06307  Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) 6.5
 LDTP16040  Glyoxalase domain-containing protein 4 (GLOD4) 6.3
 LDTP04004  Lysosomal Pro-X carboxypeptidase (PRCP) 6.3
 LDTP06333  Serum paraoxonase/arylesterase 2 (PON2) 6.2
 LDTP03189  Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) 6.2
 LDTP02005  Lysosomal acid glucosylceramidase (GBA1) 6.1
 LDTP17410  Cytochrome P450 20A1 (CYP20A1) 6.0
 LDTP06648  Lanosterol 14-alpha demethylase (CYP51A1) 6.0
 LDTP06150  DNA replication licensing factor MCM6 (MCM6) 5.9
 LDTP04519  Kinesin-like protein KIF11 (KIF11) 5.8
 LDTP01562  Peptidyl-prolyl cis-trans isomerase FKBP9 (FKBP9) 5.8
 LDTP04285  Proteasome subunit beta type-2 (PSMB2) 5.7
 LDTP01613  Sphingosine-1-phosphate lyase 1 (SGPL1) 5.7
 LDTP10188  FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) 5.7
 LDTP12490  Sphingosine kinase 2 (SPHK2) 5.7
 LDTP12279  Endoplasmic reticulum transmembrane helix translocase (ATP13A1) 5.6
 LDTP13720  Ubiquitin carboxyl-terminal hydrolase 24 (USP24) 5.6
 LDTP05862  NAD(P) transhydrogenase, mitochondrial (NNT) 5.5
 LDTP07813  Sulfhydryl oxidase 2 (QSOX2) 5.5
 LDTP00988  Protein O-GlcNAcase (OGA) 5.4
 LDTP14030  V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) 5.4
 LDTP06200  Delta(14)-sterol reductase LBR (LBR) 5.3
 LDTP12732  Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) 5.3
 LDTP04388  Palmitoyl-protein thioesterase 1 (PPT1) 5.2
 LDTP12588  Isoleucine--tRNA ligase, mitochondrial (IARS2) 5.2
 LDTP06617  Ceramide glucosyltransferase (UGCG) 5.2
 LDTP16025  Ketosamine-3-kinase (FN3KRP) 5.2
 LDTP05971  Mannosyl-oligosaccharide glucosidase (MOGS) 5.2
 LDTP00047  Threonine--tRNA ligase 2, cytoplasmic (TARS3) 5.1
 LDTP16032  Retinoid-inducible serine carboxypeptidase (SCPEP1) 5.1
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 5.1
 LDTP03900  ADP-ribosylation factor-like protein 1 (ARL1) 5.0
 LDTP15480  Prenylcysteine oxidase-like (PCYOX1L) 5.0
 LDTP07688  Spliceosome-associated protein CWC27 homolog (CWC27) 5.0
 LDTP18087  Dephospho-CoA kinase domain-containing protein (DCAKD) 5.0
 LDTP01329  Lathosterol oxidase (SC5D) 5.0
Transporter and channel
Click to Show/Hide to Show 70 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP01804  ATP synthase subunit a (MT-ATP6) 44.6
 LDTP02724  Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) 23.3
 LDTP06426  Translocating chain-associated membrane protein 1 (TRAM1) 17.1
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 16.6
 LDTP13983  Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) 16.2
 LDTP12207  Putative divalent cation/proton antiporter TMEM165 (TMEM165) 14.9
 LDTP15948  Transmembrane protein 126A (TMEM126A) 14.7
 LDTP11162  Solute carrier family 25 member 33 (SLC25A33) 14.4
 LDTP05662  Cell division cycle protein 20 homolog (CDC20) 13.6
 LDTP06275  Lysosomal-associated transmembrane protein 4A (LAPTM4A) 13.3
 LDTP13174  Mitochondrial dicarboxylate carrier (SLC25A10) 13.1
 LDTP07236  Protein GPR107 (GPR107) 12.6
 LDTP11869  Growth hormone-inducible transmembrane protein (GHITM) 12.0
 LDTP14099  Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) 11.9
 LDTP10669  Melanoma inhibitory activity protein 2 (MIA2) 11.8
 LDTP05900  Metaxin-1 (MTX1) 11.5
 LDTP06308  Mitochondrial inner membrane protein OXA1L (OXA1L) 10.3
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 10.1
 LDTP20002  Transmembrane protein 160 (TMEM160) 10.1
 LDTP14511  ATP synthase subunit g, mitochondrial (ATP5MG) 9.5
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 9.4
 LDTP01671  STARD3 N-terminal-like protein (STARD3NL) 9.4
 LDTP09968  V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) 8.8
 LDTP00262  Acetyl-coenzyme A transporter 1 (SLC33A1) 8.6
 LDTP10065  Transmembrane protein 230 (TMEM230) 8.6
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 8.5
 LDTP00562  Membrane-associated progesterone receptor component 2 (PGRMC2) 8.3
 LDTP15481  Transmembrane protein 87A (TMEM87A) 8.3
 LDTP11245  Derlin-1 (DERL1) 8.2
 LDTP09328  Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (SLC35B2) 7.9
 LDTP05511  Cytoskeleton-associated protein 4 (CKAP4) 7.9
 LDTP09333  Golgin subfamily A member 5 (GOLGA5) 7.7
 LDTP08981  Cytochrome c oxidase assembly protein COX18, mitochondrial (COX18) 7.7
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 7.5
 LDTP18448  Transmembrane 9 superfamily member 3 (TM9SF3) 7.2
 LDTP01100  Iron-sulfur clusters transporter ABCB7, mitochondrial (ABCB7) 6.9
 LDTP14200  Mitochondrial ornithine transporter 1 (SLC25A15) 6.9
 LDTP00865  Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) 6.8
 LDTP13824  Voltage-dependent anion-selective channel protein 3 (VDAC3) 6.8
 LDTP14084  Sorting and assembly machinery component 50 homolog (SAMM50) 6.7
 LDTP13320  Prenylated Rab acceptor protein 1 (RABAC1) 6.7
 LDTP12210  Mitochondrial thiamine pyrophosphate carrier (SLC25A19) 6.5
 LDTP00477  Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) 6.5
 LDTP04956  Mitochondrial import inner membrane translocase subunit Tim10 (TIMM10) 6.5
 LDTP11634  Derlin-2 (DERL2) 6.3
 LDTP11213  Nucleoporin NDC1 (NDC1) 6.3
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 6.2
 LDTP11116  45 kDa calcium-binding protein (SDF4) 6.1
 LDTP04787  Transmembrane protein 33 (TMEM33) 6.0
 LDTP03559  High affinity cationic amino acid transporter 1 (SLC7A1) 6.0
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 5.9
 LDTP01542  Golgi SNAP receptor complex member 1 (GOSR1) 5.8
 LDTP07467  Rhomboid domain-containing protein 2 (RHBDD2) 5.7
 LDTP00872  Peroxisomal membrane protein PMP34 (SLC25A17) 5.7
 LDTP18459  StAR-related lipid transfer protein 7, mitochondrial (STARD7) 5.7
 LDTP00857  Syntaxin-6 (STX6) 5.6
 LDTP03284  ATP synthase F(0) complex subunit B1, mitochondrial (ATP5PB) 5.6
 LDTP04789  Synaptojanin-2-binding protein (SYNJ2BP) 5.4
 LDTP13953  Endophilin-B1 (SH3GLB1) 5.4
 LDTP10958  Sigma non-opioid intracellular receptor 1 (SIGMAR1) 5.4
 LDTP11626  Protein YIPF3 (YIPF3) 5.3
 LDTP10499  Lipid scramblase CLPTM1L (CLPTM1L) 5.3
 LDTP09547  Scavenger receptor class B member 1 (SCARB1) 5.3
 LDTP14235  Mitochondrial carrier homolog 2 (MTCH2) 5.2
 LDTP06365  Transmembrane emp24 domain-containing protein 2 (TMED2) 5.2
 LDTP03810  ATP synthase subunit gamma, mitochondrial (ATP5F1C) 5.1
 LDTP01525  Mitochondrial proton/calcium exchanger protein (LETM1) 5.1
 LDTP11844  Protein spinster homolog 1 (SPNS1) 5.1
 LDTP07477  Transmembrane protein 214 (TMEM214) 5.0
 LDTP08881  Transmembrane protein 199 (TMEM199) 4.9
Immunoglobulin
Click to Show/Hide to Show 1 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP05572  Leukocyte surface antigen CD47 (CD47) 6.9
Other
Click to Show/Hide to Show 49 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP13311  Poly(U)-binding-splicing factor PUF60 (PUF60) 70.5
 LDTP11076  Apolipoprotein L2 (APOL2) 36.5
 LDTP01522  Reticulon-3 (RTN3) 32.7
 LDTP13086  Syntaxin-18 (STX18) 30.7
 LDTP11326  FUN14 domain-containing protein 2 (FUNDC2) 22.2
 LDTP13902  Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) 20.7
 LDTP08594  Negative elongation factor C/D (NELFCD) 16.7
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 15.8
 LDTP15067  Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) 15.8
 LDTP05160  Nucleobindin-2 (NUCB2) 14.6
 LDTP10056  Protein FAM162A (FAM162A) 10.2
 LDTP16006  Optic atrophy 3 protein (OPA3) 9.5
 LDTP12412  Reticulon-4 (RTN4) 8.9
 LDTP07891  Inhibitor of nuclear factor kappa-B kinase-interacting protein (IKBIP) 8.1
 LDTP19963  TraB domain-containing protein (TRABD) 8.1
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 7.5
 LDTP00986  Syntaxin-10 (STX10) 7.4
 LDTP15057  TBC1 domain family member 9B (TBC1D9B) 7.3
 LDTP16323  Signal recognition particle receptor subunit beta (SRPRB) 7.2
 LDTP18373  Thioredoxin-related transmembrane protein 4 (TMX4) 7.2
 LDTP10200  60S ribosomal export protein NMD3 (NMD3) 7.1
 LDTP16080  Protein FAM114A2 (FAM114A2) 7.1
 LDTP13922  Tudor and KH domain-containing protein (TDRKH) 7.0
 LDTP13755  Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) 7.0
 LDTP19021  EF-hand calcium-binding domain-containing protein 14 (EFCAB14) 7.0
 LDTP05375  Nucleobindin-1 (NUCB1) 7.0
 LDTP10403  DDRGK domain-containing protein 1 (DDRGK1) 6.9
 LDTP15623  Neuferricin (CYB5D2) 6.9
 LDTP12642  CYFIP-related Rac1 interactor B (CYRIB) 6.6
 LDTP03968  Lamina-associated polypeptide 2, isoforms beta/gamma (TMPO) 6.4
 LDTP07368  Tetratricopeptide repeat protein 19, mitochondrial (TTC19) 6.4
 LDTP18467  LYR motif-containing protein 2 (LYRM2) 6.3
 LDTP10775  Endoplasmic reticulum-Golgi intermediate compartment protein 2 (ERGIC2) 6.1
 LDTP06291  ADP-ribosylation factor-like protein 6-interacting protein 1 (ARL6IP1) 5.8
 LDTP03678  ER lumen protein-retaining receptor 2 (KDELR2) 5.7
 LDTP16192  Mitochondrial fission process protein 1 (MTFP1) 5.7
 LDTP12755  OCIA domain-containing protein 1 (OCIAD1) 5.7
 LDTP01494  Protein YIF1A (YIF1A) 5.5
 LDTP04094  Large proline-rich protein BAG6 (BAG6) 5.4
 LDTP01652  Centrosomal protein 43 (CEP43) 5.4
 LDTP11911  Golgi phosphoprotein 3 (GOLPH3) 5.4
 LDTP12379  Complex I assembly factor TIMMDC1, mitochondrial (TIMMDC1) 5.3
 LDTP04382  Kinetochore-associated protein 1 (KNTC1) 5.2
 LDTP14259  Testis-expressed protein 264 (TEX264) 5.2
 LDTP10442  Thioredoxin domain-containing protein 15 (TXNDC15) 5.2
 LDTP08364  Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) 5.2
 LDTP10218  Regulator of MON1-CCZ1 complex (RMC1) 5.1
 LDTP15253  HEAT repeat-containing protein 3 (HEATR3) 4.9
 LDTP01225  Mediator of RNA polymerase II transcription subunit 24 (MED24) 4.9

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587