Details of the Target

General Information of Target

Target ID LDTP01100
Target Name Iron-sulfur clusters transporter ABCB7, mitochondrial (ABCB7)
Gene Name ABCB7
Gene ID 22
Synonyms
ABC7; Iron-sulfur clusters transporter ABCB7, mitochondrial; ATP-binding cassette sub-family B member 7, mitochondrial; ATP-binding cassette transporter 7; ABC transporter 7 protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MALLAMHSWRWAAAAAAFEKRRHSAILIRPLVSVSGSGPQWRPHQLGALGTARAYQIPES
LKSITWQRLGKGNSGQFLDAAKALQVWPLIEKRTCWHGHAGGGLHTDPKEGLKDVDTRKI
IKAMLSYVWPKDRPDLRARVAISLGFLGGAKAMNIVVPFMFKYAVDSLNQMSGNMLNLSD
APNTVATMATAVLIGYGVSRAGAAFFNEVRNAVFGKVAQNSIRRIAKNVFLHLHNLDLGF
HLSRQTGALSKAIDRGTRGISFVLSALVFNLLPIMFEVMLVSGVLYYKCGAQFALVTLGT
LGTYTAFTVAVTRWRTRFRIEMNKADNDAGNAAIDSLLNYETVKYFNNERYEAQRYDGFL
KTYETASLKSTSTLAMLNFGQSAIFSVGLTAIMVLASQGIVAGTLTVGDLVMVNGLLFQL
SLPLNFLGTVYRETRQALIDMNTLFTLLKVDTQIKDKVMASPLQITPQTATVAFDNVHFE
YIEGQKVLSGISFEVPAGKKVAIVGGSGSGKSTIVRLLFRFYEPQKGSIYLAGQNIQDVS
LESLRRAVGVVPQDAVLFHNTIYYNLLYGNISASPEEVYAVAKLAGLHDAILRMPHGYDT
QVGERGLKLSGGEKQRVAIARAILKDPPVILYDEATSSLDSITEETILGAMKDVVKHRTS
IFIAHRLSTVVDADEIIVLDQGKVAERGTHHGLLANPHSIYSEMWHTQSSRVQNHDNPKW
EAKKENISKEEERKKLQEEIVNSVKGCGNCSC
Target Bioclass
Transporter and channel
Family
ABC transporter superfamily, ABCB family, Heavy Metal importer (TC 3.A.1.210) subfamily
Subcellular location
Mitochondrion inner membrane
Function
Exports glutathione-coordinated iron-sulfur clusters such as [2Fe-2S]-(GS)4 cluster from the mitochondria to the cytosol in an ATP-dependent manner allowing the assembly of the cytosolic iron-sulfur (Fe/S) cluster-containing proteins and participates in iron homeostasis. Moreover, through a functional complex formed of ABCB7, FECH and ABCB10, also plays a role in the cellular iron homeostasis, mitochondrial function and heme biosynthesis. In cardiomyocytes, regulates cellular iron homeostasis and cellular reactive oxygen species (ROS) levels through its interaction with COX4I1. May also play a role in hematopoiesis.
Uniprot ID
O75027
Ensemble ID
ENST00000253577.9
HGNC ID
HGNC:48

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AU565 SNV: p.E731G .
KMCH1 SNV: p.K499N .
KMS11 SNV: p.P524S .
MCC13 SNV: p.R68K; p.M153I .
MFE296 Deletion: p.P628QfsTer12
SNV: p.V165I		 .
NCIH1155 SNV: p.A333T .
SW948 SNV: p.R516K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
N1
 Probe Info  		100.00  LDD0242  [1]
m-APA
 Probe Info  		11.61  LDD0403  [2]
Acrolein
 Probe Info  		H665(0.00); H596(0.00)  LDD0222  [3]
DBIA
 Probe Info  		C752(1.02)  LDD1492  [4]
ATP probe
 Probe Info  		K719(0.00); K511(0.00); K735(0.00)  LDD0199  [5]
NHS
 Probe Info  		N.A.  LDD0010  [6]
STPyne
 Probe Info  		N.A.  LDD0009  [6]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [3]
AOyne
 Probe Info  		8.90  LDD0443  [7]
PAL-AfBPP Probe
Click To Hide/Show 38 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C056
 Probe Info  		26.35  LDD1753  [8]
C070
 Probe Info  		9.92  LDD1766  [8]
C091
 Probe Info  		13.45  LDD1782  [8]
C112
 Probe Info  		18.51  LDD1799  [8]
C134
 Probe Info  		25.63  LDD1816  [8]
C143
 Probe Info  		12.55  LDD1825  [8]
C158
 Probe Info  		19.16  LDD1838  [8]
C159
 Probe Info  		7.41  LDD1839  [8]
C161
 Probe Info  		10.85  LDD1841  [8]
C173
 Probe Info  		16.00  LDD1853  [8]
C198
 Probe Info  		23.92  LDD1874  [8]
C220
 Probe Info  		13.83  LDD1894  [8]
C235
 Probe Info  		16.91  LDD1908  [8]
C249
 Probe Info  		21.26  LDD1922  [8]
C284
 Probe Info  		51.63  LDD1954  [8]
C288
 Probe Info  		13.18  LDD1958  [8]
C289
 Probe Info  		26.54  LDD1959  [8]
C305
 Probe Info  		16.00  LDD1974  [8]
C338
 Probe Info  		14.93  LDD2001  [8]
C348
 Probe Info  		14.83  LDD2009  [8]
C349
 Probe Info  		9.25  LDD2010  [8]
C350
 Probe Info  		23.43  LDD2011  [8]
C356
 Probe Info  		11.47  LDD2017  [8]
C367
 Probe Info  		6.92  LDD2028  [8]
C382
 Probe Info  		12.21  LDD2041  [8]
C388
 Probe Info  		54.95  LDD2047  [8]
C390
 Probe Info  		45.57  LDD2049  [8]
C391
 Probe Info  		14.93  LDD2050  [8]
C394
 Probe Info  		5.50  LDD2053  [8]
C413
 Probe Info  		16.68  LDD2069  [8]
FFF probe11
 Probe Info  		10.48  LDD0471  [9]
FFF probe13
 Probe Info  		16.91  LDD0475  [9]
FFF probe14
 Probe Info  		12.46  LDD0477  [9]
FFF probe3
 Probe Info  		20.00  LDD0464  [9]
JN0003
 Probe Info  		16.95  LDD0469  [9]
STS-1
 Probe Info  		N.A.  LDD0136  [10]
STS-2
 Probe Info  		N.A.  LDD0138  [10]
OEA-DA
 Probe Info  		4.65  LDD0046  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C95(1.14)  LDD1510  [4]
 LDCM0280  AC20 HEK-293T C95(0.95)  LDD1519  [4]
 LDCM0288  AC28 HEK-293T C95(0.98)  LDD1527  [4]
 LDCM0297  AC36 HEK-293T C95(1.10)  LDD1536  [4]
 LDCM0301  AC4 HEK-293T C95(0.97)  LDD1540  [4]
 LDCM0306  AC44 HEK-293T C95(1.02)  LDD1545  [4]
 LDCM0315  AC52 HEK-293T C95(1.09)  LDD1554  [4]
 LDCM0324  AC60 HEK-293T C95(1.06)  LDD1563  [4]
 LDCM0156  Aniline NCI-H1299 11.61  LDD0403  [2]
 LDCM0108  Chloroacetamide HeLa H665(0.00); H596(0.00)  LDD0222  [3]
 LDCM0408  CL20 HEK-293T C95(1.13)  LDD1612  [4]
 LDCM0421  CL32 HEK-293T C95(1.06)  LDD1625  [4]
 LDCM0434  CL44 HEK-293T C95(1.01)  LDD1638  [4]
 LDCM0447  CL56 HEK-293T C95(1.26)  LDD1650  [4]
 LDCM0460  CL68 HEK-293T C95(1.33)  LDD1663  [4]
 LDCM0473  CL8 HEK-293T C95(2.11)  LDD1676  [4]
 LDCM0474  CL80 HEK-293T C95(1.40)  LDD1677  [4]
 LDCM0487  CL92 HEK-293T C95(1.10)  LDD1690  [4]
 LDCM0022  KB02 HEK-293T C752(1.02)  LDD1492  [4]
 LDCM0023  KB03 HEK-293T C752(1.00)  LDD1497  [4]
 LDCM0024  KB05 HEK-293T C752(1.07)  LDD1502  [4]
 LDCM0109  NEM HeLa H588(0.00); H596(0.00)  LDD0223  [3]

References

1 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
2 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
9 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
10 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
11 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570