Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C173  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 59 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP03540  Heme oxygenase 2 (HMOX2) 29.2
 LDTP04051  Nicotinamide phosphoribosyltransferase (NAMPT) 19.4
 LDTP00697  Cytochrome b5 type B (CYB5B) 17.8
 LDTP00988  Protein O-GlcNAcase (OGA) 17.5
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 17.4
 LDTP13240  Poly [ADP-ribose] polymerase 2 (PARP2) 15.2
 LDTP04712  Puromycin-sensitive aminopeptidase (NPEPPS) 14.9
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 13.7
 LDTP04375  Methionine aminopeptidase 2 (METAP2) 12.7
 LDTP04289  Very long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADVL) 12.5
 LDTP03787  Choline kinase alpha (CHKA) 12.0
 LDTP03257  Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) 11.2
 LDTP08322  Valacyclovir hydrolase (BPHL) 10.4
 LDTP12105  L-2-hydroxyglutarate dehydrogenase, mitochondrial (L2HGDH) 10.3
 LDTP13751  Exonuclease 1 (EXO1) 10.2
 LDTP06333  Serum paraoxonase/arylesterase 2 (PON2) 10.1
 LDTP08933  Chondroitin sulfate N-acetylgalactosaminyltransferase 2 (CSGALNACT2) 9.8
 LDTP19851  Isochorismatase domain-containing protein 1 (ISOC1) 9.6
 LDTP10182  TLC domain-containing protein 1 (TLCD1) 8.9
 LDTP04004  Lysosomal Pro-X carboxypeptidase (PRCP) 8.6
 LDTP03842  Squalene synthase (FDFT1) 8.5
 LDTP02359  Heme oxygenase 1 (HMOX1) 7.9
 LDTP06648  Lanosterol 14-alpha demethylase (CYP51A1) 7.9
 LDTP07688  Spliceosome-associated protein CWC27 homolog (CWC27) 7.8
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 7.6
 LDTP12279  Endoplasmic reticulum transmembrane helix translocase (ATP13A1) 7.1
 LDTP12490  Sphingosine kinase 2 (SPHK2) 7.0
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 6.9
 LDTP05396  Histone-lysine N-methyltransferase 2A (KMT2A) 6.7
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 6.7
 LDTP03608  RAC-beta serine/threonine-protein kinase (AKT2) 6.5
 LDTP07762  Hydroxysteroid dehydrogenase-like protein 2 (HSDL2) 6.4
 LDTP08455  Palmitoyltransferase ZDHHC13 (ZDHHC13) 6.4
 LDTP11187  COP9 signalosome complex subunit 4 (COPS4) 6.3
 LDTP09088  Phospholipase A2 group XV (PLA2G15) 6.3
 LDTP11273  N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) 6.2
 LDTP12639  1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (AGPAT5) 6.2
 LDTP05066  Signal peptidase complex catalytic subunit SEC11A (SEC11A) 6.2
 LDTP04285  Proteasome subunit beta type-2 (PSMB2) 6.1
 LDTP11321  Acetyl-CoA acetyltransferase, cytosolic (ACAT2) 5.9
 LDTP12363  Lysophosphatidic acid phosphatase type 6 (ACP6) 5.9
 LDTP14030  V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) 5.9
 LDTP12679  Trimethyllysine dioxygenase, mitochondrial (TMLHE) 5.9
 LDTP03560  Aldehyde dehydrogenase X, mitochondrial (ALDH1B1) 5.7
 LDTP00994  Beta-1,4-galactosyltransferase 3 (B4GALT3) 5.7
 LDTP06253  E3 ubiquitin-protein ligase SHPRH (SHPRH) 5.7
 LDTP09074  UDP-glucuronic acid decarboxylase 1 (UXS1) 5.7
 LDTP17410  Cytochrome P450 20A1 (CYP20A1) 5.6
 LDTP01335  Protein SCO1 homolog, mitochondrial (SCO1) 5.6
 LDTP08084  BRCA1-associated protein (BRAP) 5.5
 LDTP02643  Uracil-DNA glycosylase (UNG) 5.5
 LDTP06617  Ceramide glucosyltransferase (UGCG) 5.5
 LDTP04581  Holocytochrome c-type synthase (HCCS) 5.4
 LDTP04519  Kinesin-like protein KIF11 (KIF11) 5.4
 LDTP06720  Peroxisomal leader peptide-processing protease (TYSND1) 5.4
 LDTP10101  Peptidyl-prolyl cis-trans isomerase FKBP10 (FKBP10) 5.3
 LDTP01769  Dihydrofolate reductase (DHFR) 5.2
 LDTP08947  eEF1A lysine and N-terminal methyltransferase (METTL13) 5.2
 LDTP13720  Ubiquitin carboxyl-terminal hydrolase 24 (USP24) 5.2
Transporter and channel
Click to Show/Hide to Show 27 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP01100  Iron-sulfur clusters transporter ABCB7, mitochondrial (ABCB7) 16.0
 LDTP14084  Sorting and assembly machinery component 50 homolog (SAMM50) 13.6
 LDTP06426  Translocating chain-associated membrane protein 1 (TRAM1) 12.0
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 10.6
 LDTP17362  Magnesium transporter NIPA3 (NIPAL1) 10.5
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 9.6
 LDTP11406  Transmembrane protein 59 (TMEM59) 9.4
 LDTP11813  Solute carrier family 25 member 32 (SLC25A32) 9.2
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 8.6
 LDTP05900  Metaxin-1 (MTX1) 8.5
 LDTP11812  Protein ARV1 (ARV1) 7.7
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 7.7
 LDTP14200  Mitochondrial ornithine transporter 1 (SLC25A15) 7.4
 LDTP00562  Membrane-associated progesterone receptor component 2 (PGRMC2) 7.0
 LDTP03952  Reduced folate transporter (SLC19A1) 6.9
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 6.3
 LDTP17641  Solute carrier family 35 member F2 (SLC35F2) 6.1
 LDTP18153  Major facilitator superfamily domain-containing protein 3 (MFSD3) 6.0
 LDTP08881  Transmembrane protein 199 (TMEM199) 6.0
 LDTP09968  V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) 5.9
 LDTP00857  Syntaxin-6 (STX6) 5.8
 LDTP09547  Scavenger receptor class B member 1 (SCARB1) 5.5
 LDTP01671  STARD3 N-terminal-like protein (STARD3NL) 5.5
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 5.5
 LDTP07236  Protein GPR107 (GPR107) 5.4
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 5.3
 LDTP18448  Transmembrane 9 superfamily member 3 (TM9SF3) 5.3
Other
Click to Show/Hide to Show 37 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP15067  Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) 37.3
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 18.4
 LDTP08000  Dymeclin (DYM) 14.7
 LDTP15420  Mitochondrial import receptor subunit TOM5 homolog (TOMM5) 14.2
 LDTP01075  Protein CutA (CUTA) 13.6
 LDTP03520  Phosphatidylethanolamine-binding protein 1 (PEBP1) 12.9
 LDTP13902  Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) 12.0
 LDTP11076  Apolipoprotein L2 (APOL2) 10.1
 LDTP05160  Nucleobindin-2 (NUCB2) 10.0
 LDTP13755  Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) 9.8
 LDTP14058  Rap guanine nucleotide exchange factor 2 (RAPGEF2) 9.6
 LDTP13086  Syntaxin-18 (STX18) 9.1
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 8.8
 LDTP00105  Protein unc-119 homolog B (UNC119B) 8.8
 LDTP16006  Optic atrophy 3 protein (OPA3) 8.6
 LDTP03678  ER lumen protein-retaining receptor 2 (KDELR2) 8.1
 LDTP01652  Centrosomal protein 43 (CEP43) 7.7
 LDTP10965  Nucleosome assembly protein 1-like 4 (NAP1L4) 7.5
 LDTP15623  Neuferricin (CYB5D2) 7.4
 LDTP13630  Neudesin (NENF) 7.2
 LDTP02728  Nidogen-1 (NID1) 7.1
 LDTP11533  Oxysterol-binding protein-related protein 6 (OSBPL6) 7.0
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 6.9
 LDTP13311  Poly(U)-binding-splicing factor PUF60 (PUF60) 6.9
 LDTP19372  Tetratricopeptide repeat protein 38 (TTC38) 6.1
 LDTP04684  Nucleosome assembly protein 1-like 1 (NAP1L1) 6.1
 LDTP06487  Syntaxin-binding protein 2 (STXBP2) 6.0
 LDTP19860  Coiled-coil domain-containing protein 97 (CCDC97) 6.0
 LDTP05226  RNA-binding protein 3 (RBM3) 5.9
 LDTP04094  Large proline-rich protein BAG6 (BAG6) 5.9
 LDTP16192  Mitochondrial fission process protein 1 (MTFP1) 5.6
 LDTP07037  WD repeat domain phosphoinositide-interacting protein 3 (WDR45B) 5.6
 LDTP04979  U6 snRNA-associated Sm-like protein LSm3 (LSM3) 5.6
 LDTP10255  Protein Hook homolog 2 (HOOK2) 5.5
 LDTP05864  Beta-2-syntrophin (SNTB2) 5.2
 LDTP18134  Protein CUSTOS (CUSTOS) 5.2
 LDTP01522  Reticulon-3 (RTN3) 5.2

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587