Details of the Target

General Information of Target

Target ID LDTP00994
Target Name Beta-1,4-galactosyltransferase 3 (B4GALT3)
Gene Name B4GALT3
Gene ID 8703
Synonyms
Beta-1,4-galactosyltransferase 3; Beta-1,4-GalTase 3; Beta4Gal-T3; b4Gal-T3; EC 2.4.1.-; Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase; EC 2.4.1.38; N-acetyllactosamine synthase; EC 2.4.1.90; Nal synthase; Neolactotriaosylceramide beta-1,4-galactosyltransferase; EC 2.4.1.275; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLRRLLERPCTLALLVGSQLAVMMYLSLGGFRSLSALFGRDQGPTFDYSHPRDVYSNLSH
LPGAPGGPPAPQGLPYCPERSPLLVGPVSVSFSPVPSLAEIVERNPRVEPGGRYRPAGCE
PRSRTAIIVPHRAREHHLRLLLYHLHPFLQRQQLAYGIYVIHQAGNGTFNRAKLLNVGVR
EALRDEEWDCLFLHDVDLLPENDHNLYVCDPRGPRHVAVAMNKFGYSLPYPQYFGGVSAL
TPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKISRPPTSVGHYKMVKHRGDKGNEEN
PHRFDLLVRTQNSWTQDGMNSLTYQLLARELGPLYTNITADIGTDPRGPRAPSGPRYPPG
SSQAFRQEMLQRRPPARPGPLSTANHTALRGSH
Target Bioclass
Enzyme
Family
Glycosyltransferase 7 family
Subcellular location
Golgi apparatus, Golgi stack membrane
Function Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Uniprot ID
O60512
Ensemble ID
ENST00000319769.10
HGNC ID
HGNC:926

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL27 SNV: p.M24K .
HCC1419 SNV: p.G225Ter .
HKGZCC SNV: p.R347W .
JURKAT Deletion: p.G67VfsTer18
SNV: p.R107G		 .
KATOIII SNV: p.L246V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Acrolein
 Probe Info  		N.A.  LDD0227  [1]
DBIA
 Probe Info  		C119(1.06)  LDD0547  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [3]
PAL-AfBPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C004
 Probe Info  		17.88  LDD1714  [4]
C055
 Probe Info  		13.27  LDD1752  [4]
C056
 Probe Info  		18.38  LDD1753  [4]
C112
 Probe Info  		17.51  LDD1799  [4]
C169
 Probe Info  		26.35  LDD1849  [4]
C170
 Probe Info  		16.45  LDD1850  [4]
C173
 Probe Info  		5.70  LDD1853  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C77(1.07); C119(0.98)  LDD1508  [5]
 LDCM0230  AC113 HCT 116 C119(1.06)  LDD0547  [2]
 LDCM0231  AC114 HCT 116 C119(1.09)  LDD0548  [2]
 LDCM0232  AC115 HCT 116 C119(1.24)  LDD0549  [2]
 LDCM0233  AC116 HCT 116 C119(1.16)  LDD0550  [2]
 LDCM0234  AC117 HCT 116 C119(1.41)  LDD0551  [2]
 LDCM0235  AC118 HCT 116 C119(1.13)  LDD0552  [2]
 LDCM0236  AC119 HCT 116 C119(1.12)  LDD0553  [2]
 LDCM0237  AC12 HEK-293T C77(1.04); C119(1.03)  LDD1510  [5]
 LDCM0238  AC120 HCT 116 C119(1.16)  LDD0555  [2]
 LDCM0239  AC121 HCT 116 C119(1.35)  LDD0556  [2]
 LDCM0240  AC122 HCT 116 C119(1.24)  LDD0557  [2]
 LDCM0241  AC123 HCT 116 C119(1.71)  LDD0558  [2]
 LDCM0242  AC124 HCT 116 C119(1.18)  LDD0559  [2]
 LDCM0243  AC125 HCT 116 C119(1.02)  LDD0560  [2]
 LDCM0244  AC126 HCT 116 C119(0.97)  LDD0561  [2]
 LDCM0245  AC127 HCT 116 C119(1.15)  LDD0562  [2]
 LDCM0259  AC14 HEK-293T C119(1.02)  LDD1512  [5]
 LDCM0277  AC18 HEK-293T C77(1.16); C119(0.94)  LDD1516  [5]
 LDCM0279  AC2 HEK-293T C77(1.15); C119(0.97)  LDD1518  [5]
 LDCM0280  AC20 HEK-293T C77(1.06); C119(0.99)  LDD1519  [5]
 LDCM0281  AC21 HEK-293T C119(1.29)  LDD1520  [5]
 LDCM0282  AC22 HEK-293T C119(0.99)  LDD1521  [5]
 LDCM0284  AC24 HEK-293T C77(0.90); C119(1.01)  LDD1523  [5]
 LDCM0285  AC25 HCT 116 C119(1.08)  LDD0602  [2]
 LDCM0286  AC26 HCT 116 C119(0.89)  LDD0603  [2]
 LDCM0287  AC27 HCT 116 C119(0.73)  LDD0604  [2]
 LDCM0288  AC28 HCT 116 C119(0.83)  LDD0605  [2]
 LDCM0289  AC29 HCT 116 C119(0.78)  LDD0606  [2]
 LDCM0291  AC30 HCT 116 C119(0.78)  LDD0608  [2]
 LDCM0292  AC31 HCT 116 C119(0.80)  LDD0609  [2]
 LDCM0293  AC32 HCT 116 C119(0.79)  LDD0610  [2]
 LDCM0294  AC33 HCT 116 C119(0.95)  LDD0611  [2]
 LDCM0295  AC34 HCT 116 C119(0.93)  LDD0612  [2]
 LDCM0296  AC35 HCT 116 C119(1.14)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C119(1.35)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C119(1.19)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C119(1.02)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C119(0.98)  LDD0617  [2]
 LDCM0301  AC4 HEK-293T C77(1.43); C119(1.04)  LDD1540  [5]
 LDCM0302  AC40 HCT 116 C119(1.05)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C119(1.03)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C119(1.06)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C119(1.11)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C119(1.36)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C119(1.02)  LDD0624  [2]
 LDCM0308  AC46 HCT 116 C119(0.75)  LDD0625  [2]
 LDCM0309  AC47 HCT 116 C119(0.78)  LDD0626  [2]
 LDCM0310  AC48 HCT 116 C119(0.69)  LDD0627  [2]
 LDCM0311  AC49 HCT 116 C119(0.76)  LDD0628  [2]
 LDCM0312  AC5 HEK-293T C119(0.84)  LDD1551  [5]
 LDCM0313  AC50 HCT 116 C119(0.69)  LDD0630  [2]
 LDCM0314  AC51 HCT 116 C119(0.73)  LDD0631  [2]
 LDCM0315  AC52 HCT 116 C119(0.72)  LDD0632  [2]
 LDCM0316  AC53 HCT 116 C119(1.01)  LDD0633  [2]
 LDCM0317  AC54 HCT 116 C119(0.73)  LDD0634  [2]
 LDCM0318  AC55 HCT 116 C119(1.16)  LDD0635  [2]
 LDCM0319  AC56 HCT 116 C119(0.83)  LDD0636  [2]
 LDCM0321  AC58 HEK-293T C77(1.07); C119(1.03)  LDD1560  [5]
 LDCM0323  AC6 HEK-293T C119(1.01)  LDD1562  [5]
 LDCM0324  AC60 HEK-293T C77(0.95); C119(1.09)  LDD1563  [5]
 LDCM0325  AC61 HEK-293T C119(0.96)  LDD1564  [5]
 LDCM0326  AC62 HEK-293T C119(0.99)  LDD1565  [5]
 LDCM0328  AC64 HEK-293T C77(1.17); C119(0.98)  LDD1567  [5]
 LDCM0345  AC8 HEK-293T C77(1.05); C119(1.01)  LDD1569  [5]
 LDCM0248  AKOS034007472 HEK-293T C119(0.96)  LDD1511  [5]
 LDCM0275  AKOS034007705 HEK-293T C77(1.08); C119(1.07)  LDD1514  [5]
 LDCM0020  ARS-1620 HCC44 C119(1.08)  LDD2171  [2]
 LDCM0368  CL10 HEK-293T C119(1.00)  LDD1572  [5]
 LDCM0369  CL100 HEK-293T C119(0.97)  LDD1573  [5]
 LDCM0371  CL102 HEK-293T C77(1.07)  LDD1575  [5]
 LDCM0373  CL104 HEK-293T C119(0.96)  LDD1577  [5]
 LDCM0375  CL106 HEK-293T C77(0.90)  LDD1579  [5]
 LDCM0377  CL108 HEK-293T C119(0.84)  LDD1581  [5]
 LDCM0380  CL110 HEK-293T C77(1.10)  LDD1584  [5]
 LDCM0382  CL112 HCT 116 C119(0.83)  LDD0699  [2]
 LDCM0383  CL113 HCT 116 C119(0.71)  LDD0700  [2]
 LDCM0384  CL114 HCT 116 C119(0.76)  LDD0701  [2]
 LDCM0385  CL115 HCT 116 C119(0.85)  LDD0702  [2]
 LDCM0386  CL116 HCT 116 C119(0.93)  LDD0703  [2]
 LDCM0387  CL117 HCT 116 C119(1.06)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C119(1.16)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C119(1.20)  LDD0706  [2]
 LDCM0390  CL12 HEK-293T C77(0.89); C119(1.01)  LDD1594  [5]
 LDCM0391  CL120 HCT 116 C119(1.06)  LDD0708  [2]
 LDCM0392  CL121 HCT 116 C119(0.82)  LDD0709  [2]
 LDCM0393  CL122 HCT 116 C119(0.83)  LDD0710  [2]
 LDCM0394  CL123 HCT 116 C119(0.86)  LDD0711  [2]
 LDCM0395  CL124 HCT 116 C119(0.73)  LDD0712  [2]
 LDCM0397  CL126 HEK-293T C77(1.19)  LDD1601  [5]
 LDCM0399  CL128 HEK-293T C119(0.96)  LDD1603  [5]
 LDCM0401  CL14 HEK-293T C77(1.11)  LDD1605  [5]
 LDCM0403  CL16 HEK-293T C119(1.06)  LDD1607  [5]
 LDCM0405  CL18 HEK-293T C77(1.01); C119(1.00)  LDD1609  [5]
 LDCM0407  CL2 HEK-293T C77(1.11)  LDD1611  [5]
 LDCM0408  CL20 HEK-293T C77(1.02); C119(1.08)  LDD1612  [5]
 LDCM0409  CL21 HEK-293T C119(0.90)  LDD1613  [5]
 LDCM0410  CL22 HEK-293T C119(1.02)  LDD1614  [5]
 LDCM0412  CL24 HEK-293T C77(1.06); C119(0.98)  LDD1616  [5]
 LDCM0414  CL26 HEK-293T C77(0.92)  LDD1618  [5]
 LDCM0416  CL28 HEK-293T C119(0.95)  LDD1620  [5]
 LDCM0419  CL30 HEK-293T C77(1.04); C119(1.04)  LDD1623  [5]
 LDCM0421  CL32 HEK-293T C77(0.93); C119(1.17)  LDD1625  [5]
 LDCM0422  CL33 HEK-293T C119(1.09)  LDD1626  [5]
 LDCM0423  CL34 HEK-293T C119(0.97)  LDD1627  [5]
 LDCM0425  CL36 HEK-293T C77(0.87); C119(1.01)  LDD1629  [5]
 LDCM0429  CL4 HEK-293T C119(0.97)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C119(0.88)  LDD1634  [5]
 LDCM0432  CL42 HEK-293T C77(0.92); C119(1.03)  LDD1636  [5]
 LDCM0434  CL44 HEK-293T C77(0.92); C119(1.08)  LDD1638  [5]
 LDCM0435  CL45 HEK-293T C119(1.25)  LDD1639  [5]
 LDCM0436  CL46 HEK-293T C119(1.02)  LDD1640  [5]
 LDCM0438  CL48 HEK-293T C77(1.02); C119(1.00)  LDD1642  [5]
 LDCM0441  CL50 HEK-293T C77(1.11)  LDD1645  [5]
 LDCM0443  CL52 HEK-293T C119(1.02)  LDD1646  [5]
 LDCM0445  CL54 HEK-293T C77(1.28); C119(1.07)  LDD1648  [5]
 LDCM0447  CL56 HEK-293T C77(0.91); C119(1.14)  LDD1650  [5]
 LDCM0448  CL57 HEK-293T C119(0.96)  LDD1651  [5]
 LDCM0449  CL58 HEK-293T C119(1.05)  LDD1652  [5]
 LDCM0451  CL6 HEK-293T C77(1.13); C119(0.96)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C77(0.94); C119(1.01)  LDD1655  [5]
 LDCM0454  CL62 HEK-293T C77(1.17)  LDD1657  [5]
 LDCM0456  CL64 HEK-293T C119(0.96)  LDD1659  [5]
 LDCM0458  CL66 HEK-293T C77(1.17); C119(1.04)  LDD1661  [5]
 LDCM0460  CL68 HEK-293T C77(0.98); C119(1.08)  LDD1663  [5]
 LDCM0461  CL69 HEK-293T C119(1.48)  LDD1664  [5]
 LDCM0463  CL70 HEK-293T C119(1.01)  LDD1666  [5]
 LDCM0465  CL72 HEK-293T C77(0.99); C119(1.02)  LDD1668  [5]
 LDCM0467  CL74 HEK-293T C77(0.98)  LDD1670  [5]
 LDCM0469  CL76 HEK-293T C119(0.96)  LDD1672  [5]
 LDCM0471  CL78 HEK-293T C77(0.99); C119(0.97)  LDD1674  [5]
 LDCM0473  CL8 HEK-293T C77(1.15); C119(1.30)  LDD1676  [5]
 LDCM0474  CL80 HEK-293T C77(1.00); C119(1.05)  LDD1677  [5]
 LDCM0475  CL81 HEK-293T C119(0.87)  LDD1678  [5]
 LDCM0476  CL82 HEK-293T C119(0.98)  LDD1679  [5]
 LDCM0478  CL84 HEK-293T C77(0.90); C119(1.06)  LDD1681  [5]
 LDCM0480  CL86 HEK-293T C77(0.97)  LDD1683  [5]
 LDCM0482  CL88 HEK-293T C119(1.06)  LDD1685  [5]
 LDCM0484  CL9 HEK-293T C119(1.07)  LDD1687  [5]
 LDCM0485  CL90 HEK-293T C77(1.16); C119(1.03)  LDD1688  [5]
 LDCM0487  CL92 HEK-293T C77(1.14); C119(1.08)  LDD1690  [5]
 LDCM0488  CL93 HEK-293T C119(0.93)  LDD1691  [5]
 LDCM0489  CL94 HEK-293T C119(1.03)  LDD1692  [5]
 LDCM0491  CL96 HEK-293T C77(0.98); C119(1.06)  LDD1694  [5]
 LDCM0493  CL98 HEK-293T C77(1.26)  LDD1696  [5]
 LDCM0427  Fragment51 HEK-293T C77(1.12)  LDD1631  [5]
 LDCM0109  NEM HeLa N.A.  LDD0227  [1]
 LDCM0021  THZ1 HCT 116 C119(1.08)  LDD2173  [2]

References

1 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402