Details of the Target

General Information of Target

Target ID LDTP08933
Target Name Chondroitin sulfate N-acetylgalactosaminyltransferase 2 (CSGALNACT2)
Gene Name CSGALNACT2
Gene ID 55454
Synonyms
CHGN2; GALNACT2; Chondroitin sulfate N-acetylgalactosaminyltransferase 2; EC 2.4.1.174; Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 2; Beta4GalNAcT-2; GalNAcT-2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPRRGLILHTRTHWLLLGLALLCSLVLFMYLLECAPQTDGNASLPGVVGENYGKEYYQAL
LQEQEEHYQTRATSLKRQIAQLKQELQEMSEKMRSLQERRNVGANGIGYQSNKEQAPSDL
LEFLHSQIDKAEVSIGAKLPSEYGVIPFESFTLMKVFQLEMGLTRHPEEKPVRKDKRDEL
VEVIEAGLEVINNPDEDDEQEDEEGPLGEKLIFNENDFVEGYYRTERDKGTQYELFFKKA
DLTEYRHVTLFRPFGPLMKVKSEMIDITRSIINIIVPLAERTEAFVQFMQNFRDVCIHQD
KKIHLTVVYFGKEGLSKVKSILESVTSESNFHNYTLVSLNEEFNRGRGLNVGARAWDKGE
VLMFFCDVDIYFSAEFLNSCRLNAEPGKKVFYPVVFSLYNPAIVYANQEVPPPVEQQLVH
KKDSGFWRDFGFGMTCQYRSDFLTIGGFDMEVKGWGGEDVHLYRKYLHGDLIVIRTPVPG
LFHLWHEKRCADELTPEQYRMCIQSKAMNEASHSHLGMLVFREEIETHLHKQAYRTNSEA
VG
Target Bioclass
Enzyme
Family
Chondroitin N-acetylgalactosaminyltransferase family
Subcellular location
Golgi apparatus, Golgi stack membrane
Function
Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains.
Uniprot ID
Q8N6G5
Ensemble ID
ENST00000374466.4
HGNC ID
HGNC:24292

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AU565 SNV: p.E50G .
CAL72 SNV: p.W14C .
CORL23 SNV: p.K301N .
HCT15 SNV: p.Q58R; p.Y143H .
HT115 SNV: p.F372L .
LS180 SNV: p.E50G .
MFE296 SNV: p.F391V .
MFE319 SNV: p.C34Y; p.E189G .
RKO SNV: p.Q417R .
TYKNU SNV: p.D119E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C490(1.65)  LDD1576  [1]
PAL-AfBPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C070
 Probe Info  		8.63  LDD1766  [2]
C169
 Probe Info  		49.52  LDD1849  [2]
C170
 Probe Info  		19.70  LDD1850  [2]
C171
 Probe Info  		5.10  LDD1851  [2]
C173
 Probe Info  		9.78  LDD1853  [2]
C174
 Probe Info  		6.87  LDD1854  [2]
C201
 Probe Info  		99.73  LDD1877  [2]
C204
 Probe Info  		92.41  LDD1879  [2]
C205
 Probe Info  		13.74  LDD1880  [2]
C206
 Probe Info  		99.73  LDD1881  [2]
C207
 Probe Info  		47.18  LDD1882  [2]
C210
 Probe Info  		56.49  LDD1884  [2]
C211
 Probe Info  		11.88  LDD1885  [2]
C213
 Probe Info  		19.29  LDD1887  [2]
C355
 Probe Info  		23.59  LDD2016  [2]
C356
 Probe Info  		11.16  LDD2017  [2]
C357
 Probe Info  		6.19  LDD2018  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0372  CL103 HEK-293T C490(1.65)  LDD1576  [1]
 LDCM0376  CL107 HEK-293T C490(1.04)  LDD1580  [1]
 LDCM0381  CL111 HEK-293T C490(1.19)  LDD1585  [1]
 LDCM0385  CL115 HEK-293T C490(1.14)  LDD1589  [1]
 LDCM0389  CL119 HEK-293T C490(1.08)  LDD1593  [1]
 LDCM0394  CL123 HEK-293T C490(1.10)  LDD1598  [1]
 LDCM0398  CL127 HEK-293T C490(1.26)  LDD1602  [1]
 LDCM0402  CL15 HEK-293T C490(0.86)  LDD1606  [1]
 LDCM0415  CL27 HEK-293T C490(0.83)  LDD1619  [1]
 LDCM0418  CL3 HEK-293T C490(0.99)  LDD1622  [1]
 LDCM0428  CL39 HEK-293T C490(1.18)  LDD1632  [1]
 LDCM0455  CL63 HEK-293T C490(1.03)  LDD1658  [1]
 LDCM0481  CL87 HEK-293T C490(1.42)  LDD1684  [1]
 LDCM0494  CL99 HEK-293T C490(1.35)  LDD1697  [1]
 LDCM0495  E2913 HEK-293T C490(0.89)  LDD1698  [1]
 LDCM0468  Fragment33 HEK-293T C490(1.33)  LDD1671  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
NADH-cytochrome b5 reductase 3 (CYB5R3) Flavoprotein pyridine nucleotide cytochrome reductase family P00387
Estradiol 17-beta-dehydrogenase 11 (HSD17B11) Short-chain dehydrogenases/reductases (SDR) family Q8NBQ5
ADP-ribosylation factor-like protein 13B (ARL13B) Arf family Q3SXY8
Protein ATP1B4 (ATP1B4) X(+)/potassium ATPases subunit beta family Q9UN42
Transporter and channel
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Hippocampus abundant transcript-like protein 1 (MFSD14B) Major facilitator superfamily Q5SR56
Synaptotagmin-1 (SYT1) Synaptotagmin family P21579
Solute carrier family 35 member E3 (SLC35E3) TPT transporter family Q7Z769
Barttin (BSND) . Q8WZ55
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) BZIP family Q96BA8
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Probable G-protein coupled receptor 152 (GPR152) G-protein coupled receptor 1 family Q8TDT2
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Claudin-2 (CLDN2) Claudin family P57739
Nesprin-4 (SYNE4) Nesprin family Q8N205
Reticulophagy regulator 3 (RETREG3) RETREG family Q86VR2
Serine-rich single-pass membrane protein 1 (SSMEM1) . Q8WWF3
Transmembrane protein 143 (TMEM143) . Q96AN5
Transmembrane protein 52B (TMEM52B) . Q4KMG9

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587