Details of the Probe

General Information of Probe

Probe ID
LDPC0588
Probe Name
C367
Synonyms
Z3433405043
Probe Type
PAL-AfBPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 345.35
Lipid-water partition coefficient (xlogp) 2.7
Rotatable Bond Count (rotbonds) 7
Hydrogen Bond Donor Count (hbonddonor) 1
Hydrogen Bond Acceptor Count (hbondacc) 6
Chemical Identifiers
Formula
C17H17F2N5O
IUPAC Name
N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]-2-(5,6-difluoro-2-methylbenzimidazol-1-yl)acetamide
Canonical SMILES
CC1=NC2=CC(=C(C=C2N1CC(=O)NCCC3(N=N3)CCC#C)F)F
InChI
InChI=1S/C17H17F2N5O/c1-3-4-5-17(22-23-17)6-7-20-16(25)10-24-11(2)21-14-8-12(18)13(19)9-15(14)24/h1,8-9H,4-7,10H2,2H3,(H,20,25)
InChIKey
LJKKVUYUOUONNK-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

79 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
3-ketodihydrosphingosine reductase (KDSR) LDTP05476 6.68  LDD2028 
5'-nucleotidase domain-containing protein 1 (NT5DC1) LDTP17247 26.54  LDD2028 
ADP-dependent glucokinase (ADPGK) LDTP11127 7.62  LDD2028 
ADP-ribosylation factor-like protein 1 (ARL1) LDTP03900 5.03  LDD2028 
Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) LDTP04437 13.27  LDD2028 
ATP-dependent RNA helicase DHX29 (DHX29) LDTP08064 6.63  LDD2028 
Beta-1,4-glucuronyltransferase 1 (B4GAT1) LDTP00783 6.63  LDD2028 
CAAX prenyl protease 1 homolog (ZMPSTE24) LDTP01328 9.45  LDD2028 
Carbohydrate sulfotransferase 14 (CHST14) LDTP09096 9.58  LDD2028 
CDGSH iron-sulfur domain-containing protein 1 (CISD1) LDTP12873 8.00  LDD2028 
Ceramide glucosyltransferase (UGCG) LDTP06617 5.17  LDD2028 
Ceramide synthase 2 (CERS2) LDTP10329 7.94  LDD2028 
Choline kinase alpha (CHKA) LDTP03787 9.38  LDD2028 
COP9 signalosome complex subunit 4 (COPS4) LDTP11187 46.53  LDD2028 
Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) LDTP03189 6.19  LDD2028 
Cytochrome b5 type B (CYB5B) LDTP00697 21.11  LDD2028 
Cytochrome c oxidase subunit 2 (MT-CO2) LDTP01773 6.96  LDD2028 
Cytochrome P450 20A1 (CYP20A1) LDTP17410 6.02  LDD2028 
DCN1-like protein 4 (DCUN1D4) LDTP09799 7.36  LDD2028 
Delta(14)-sterol reductase LBR (LBR) LDTP06200 5.31  LDD2028 
Delta(24)-sterol reductase (DHCR24) LDTP06375 7.16  LDD2028 
Dephospho-CoA kinase domain-containing protein (DCAKD) LDTP18087 4.96  LDD2028 
DNA replication licensing factor MCM6 (MCM6) LDTP06150 5.94  LDD2028 
E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) LDTP10699 34.78  LDD2028 
Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial (ETFDH) LDTP06511 8.28  LDD2028 
Endoplasmic reticulum transmembrane helix translocase (ATP13A1) LDTP12279 5.62  LDD2028 
Epoxide hydrolase 1 (EPHX1) LDTP02175 11.00  LDD2028 
Ethanolamine kinase 1 (ETNK1) LDTP12197 8.34  LDD2028 
Exonuclease 1 (EXO1) LDTP13751 7.84  LDD2028 
FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) LDTP10188 5.66  LDD2028 
Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM) LDTP12253 13.09  LDD2028 
Glycylpeptide N-tetradecanoyltransferase 1 (NMT1) LDTP03537 6.82  LDD2028 
Glyoxalase domain-containing protein 4 (GLOD4) LDTP16040 6.32  LDD2028 
Heme oxygenase 2 (HMOX2) LDTP03540 12.55  LDD2028 
Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN) LDTP12732 5.31  LDD2028 
Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) LDTP06787 7.26  LDD2028 
Isochorismatase domain-containing protein 1 (ISOC1) LDTP19851 18.77  LDD2028 
Isoleucine--tRNA ligase, mitochondrial (IARS2) LDTP12588 5.21  LDD2028 
Ketosamine-3-kinase (FN3KRP) LDTP16025 5.17  LDD2028 
Kinesin-like protein KIF11 (KIF11) LDTP04519 5.82  LDD2028 
Lanosterol 14-alpha demethylase (CYP51A1) LDTP06648 6.02  LDD2028 
Lathosterol oxidase (SC5D) LDTP01329 4.96  LDD2028 
Lysophosphatidic acid phosphatase type 6 (ACP6) LDTP12363 7.67  LDD2028 
Lysosomal acid glucosylceramidase (GBA1) LDTP02005 6.06  LDD2028 
Lysosomal Pro-X carboxypeptidase (PRCP) LDTP04004 6.28  LDD2028 
Mannosyl-oligosaccharide glucosidase (MOGS) LDTP05971 5.17  LDD2028 
NAD(P) transhydrogenase, mitochondrial (NNT) LDTP05862 5.50  LDD2028 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 11.79  LDD2028 
NADH dehydrogenase 1 beta subcomplex subunit 5, mitochondrial (NDUFB5) LDTP00831 19.29  LDD2028 
NADH dehydrogenase 1 beta subcomplex subunit 9 (NDUFB9) LDTP14276 6.87  LDD2028 
NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) LDTP01982 10.34  LDD2028 
Nicotinamide phosphoribosyltransferase (NAMPT) LDTP04051 8.46  LDD2028 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 5.24  LDD2028 
Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10) LDTP12628 10.20  LDD2028 
Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) LDTP14218 8.17  LDD2028 
Peptidyl-prolyl cis-trans isomerase FKBP9 (FKBP9) LDTP01562 5.82  LDD2028 
Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) LDTP06307 6.50  LDD2028 
Peroxisomal N(1)-acetyl-spermine/spermidine oxidase (PAOX) LDTP07620 6.96  LDD2028 
Prenylcysteine oxidase 1 (PCYOX1) LDTP13282 5.06  LDD2028 
Prenylcysteine oxidase-like (PCYOX1L) LDTP15480 5.03  LDD2028 
Proteasome subunit beta type-2 (PSMB2) LDTP04285 5.74  LDD2028 
Protein O-GlcNAcase (OGA) LDTP00988 5.43  LDD2028 
Protein SCO1 homolog, mitochondrial (SCO1) LDTP01335 10.63  LDD2028 
Protein-S-isoprenylcysteine O-methyltransferase (ICMT) LDTP01039 9.38  LDD2028 
Puromycin-sensitive aminopeptidase (NPEPPS) LDTP04712 13.36  LDD2028 
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta (PDE6D) LDTP00907 29.24  LDD2028 
Retinoid-inducible serine carboxypeptidase (SCPEP1) LDTP16032 5.10  LDD2028 
Ribosomal protein S6 kinase alpha-3 (RPS6KA3) LDTP04471 6.87  LDD2028 
Serum paraoxonase/arylesterase 2 (PON2) LDTP06333 6.23  LDD2028 
Signal peptidase complex catalytic subunit SEC11A (SEC11A) LDTP05066 16.00  LDD2028 
Sphingosine kinase 2 (SPHK2) LDTP12490 5.66  LDD2028 
Sphingosine-1-phosphate lyase 1 (SGPL1) LDTP01613 5.74  LDD2028 
Spliceosome-associated protein CWC27 homolog (CWC27) LDTP07688 5.03  LDD2028 
Sulfhydryl oxidase 2 (QSOX2) LDTP07813 5.46  LDD2028 
Threonine--tRNA ligase 2, cytoplasmic (TARS3) LDTP00047 5.13  LDD2028 
Threonine--tRNA ligase, mitochondrial (TARS2) LDTP11319 10.56  LDD2028 
Ubiquitin carboxyl-terminal hydrolase 24 (USP24) LDTP13720 5.58  LDD2028 
V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) LDTP14030 5.39  LDD2028 
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADVL) LDTP04289 7.26  LDD2028 
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⏷ Show the Full List of 79 Enzyme
70 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
45 kDa calcium-binding protein (SDF4) LDTP11116 6.06  LDD2028 
Acetyl-coenzyme A transporter 1 (SLC33A1) LDTP00262 8.63  LDD2028 
Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (SLC35B2) LDTP09328 7.89  LDD2028 
ATP synthase F(0) complex subunit B1, mitochondrial (ATP5PB) LDTP03284 5.58  LDD2028 
ATP synthase subunit a (MT-ATP6) LDTP01804 44.63  LDD2028 
ATP synthase subunit g, mitochondrial (ATP5MG) LDTP14511 9.51  LDD2028 
ATP synthase subunit gamma, mitochondrial (ATP5F1C) LDTP03810 5.13  LDD2028 
Cell division cycle protein 20 homolog (CDC20) LDTP05662 13.64  LDD2028 
Cytochrome c oxidase assembly protein COX18, mitochondrial (COX18) LDTP08981 7.67  LDD2028 
Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) LDTP02724 23.26  LDD2028 
Cytoskeleton-associated protein 4 (CKAP4) LDTP05511 7.89  LDD2028 
Derlin-1 (DERL1) LDTP11245 8.22  LDD2028 
Derlin-2 (DERL2) LDTP11634 6.28  LDD2028 
Endophilin-B1 (SH3GLB1) LDTP13953 5.35  LDD2028 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 9.45  LDD2028 
Golgi SNAP receptor complex member 1 (GOSR1) LDTP01542 5.78  LDD2028 
Golgin subfamily A member 5 (GOLGA5) LDTP09333 7.73  LDD2028 
Growth hormone-inducible transmembrane protein (GHITM) LDTP11869 11.96  LDD2028 
High affinity cationic amino acid transporter 1 (SLC7A1) LDTP03559 5.98  LDD2028 
Iron-sulfur clusters transporter ABCB7, mitochondrial (ABCB7) LDTP01100 6.92  LDD2028 
Lipid scramblase CLPTM1L (CLPTM1L) LDTP10499 5.28  LDD2028 
Lysosomal-associated transmembrane protein 4A (LAPTM4A) LDTP06275 13.27  LDD2028 
Melanoma inhibitory activity protein 2 (MIA2) LDTP10669 11.79  LDD2028 
Membrane-associated progesterone receptor component 2 (PGRMC2) LDTP00562 8.34  LDD2028 
Metaxin-1 (MTX1) LDTP05900 11.47  LDD2028 
Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) LDTP00865 6.82  LDD2028 
Mitochondrial carrier homolog 2 (MTCH2) LDTP14235 5.17  LDD2028 
Mitochondrial dicarboxylate carrier (SLC25A10) LDTP13174 13.09  LDD2028 
Mitochondrial import inner membrane translocase subunit Tim10 (TIMM10) LDTP04956 6.45  LDD2028 
Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) LDTP13983 16.22  LDD2028 
Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) LDTP10913 5.94  LDD2028 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 8.51  LDD2028 
Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) LDTP14099 11.88  LDD2028 
Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) LDTP00477 6.50  LDD2028 
Mitochondrial inner membrane protein OXA1L (OXA1L) LDTP06308 10.34  LDD2028 
Mitochondrial ornithine transporter 1 (SLC25A15) LDTP14200 6.92  LDD2028 
Mitochondrial proton/calcium exchanger protein (LETM1) LDTP01525 5.10  LDD2028 
Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) LDTP07885 16.56  LDD2028 
Mitochondrial thiamine pyrophosphate carrier (SLC25A19) LDTP12210 6.54  LDD2028 
Mpv17-like protein 2 (MPV17L2) LDTP14952 7.46  LDD2028 
Nucleoporin NDC1 (NDC1) LDTP11213 6.28  LDD2028 
Peroxisomal membrane protein PMP34 (SLC25A17) LDTP00872 5.66  LDD2028 
Prenylated Rab acceptor protein 1 (RABAC1) LDTP13320 6.68  LDD2028 
Protein GPR107 (GPR107) LDTP07236 12.64  LDD2028 
Protein spinster homolog 1 (SPNS1) LDTP11844 5.06  LDD2028 
Protein YIPF3 (YIPF3) LDTP11626 5.31  LDD2028 
Putative divalent cation/proton antiporter TMEM165 (TMEM165) LDTP12207 14.93  LDD2028 
Rhomboid domain-containing protein 2 (RHBDD2) LDTP07467 5.74  LDD2028 
Scavenger receptor class B member 1 (SCARB1) LDTP09547 5.28  LDD2028 
Sigma non-opioid intracellular receptor 1 (SIGMAR1) LDTP10958 5.35  LDD2028 
Solute carrier family 25 member 33 (SLC25A33) LDTP11162 14.42  LDD2028 
Sorting and assembly machinery component 50 homolog (SAMM50) LDTP14084 6.73  LDD2028 
StAR-related lipid transfer protein 7, mitochondrial (STARD7) LDTP18459 5.66  LDD2028 
STARD3 N-terminal-like protein (STARD3NL) LDTP01671 9.38  LDD2028 
Synaptojanin-2-binding protein (SYNJ2BP) LDTP04789 5.39  LDD2028 
Syntaxin-6 (STX6) LDTP00857 5.62  LDD2028 
Translocating chain-associated membrane protein 1 (TRAM1) LDTP06426 17.15  LDD2028 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 10.06  LDD2028 
Transmembrane 9 superfamily member 3 (TM9SF3) LDTP18448 7.21  LDD2028 
Transmembrane emp24 domain-containing protein 2 (TMED2) LDTP06365 5.17  LDD2028 
Transmembrane protein 126A (TMEM126A) LDTP15948 14.72  LDD2028 
Transmembrane protein 160 (TMEM160) LDTP20002 10.06  LDD2028 
Transmembrane protein 199 (TMEM199) LDTP08881 4.92  LDD2028 
Transmembrane protein 214 (TMEM214) LDTP07477 4.96  LDD2028 
Transmembrane protein 230 (TMEM230) LDTP10065 8.57  LDD2028 
Transmembrane protein 33 (TMEM33) LDTP04787 6.02  LDD2028 
Transmembrane protein 87A (TMEM87A) LDTP15481 8.28  LDD2028 
V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) LDTP09968 8.75  LDD2028 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 6.19  LDD2028 
Voltage-dependent anion-selective channel protein 3 (VDAC3) LDTP13824 6.82  LDD2028 
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⏷ Show the Full List of 70 Transporter and channel
1 Immunoglobulin Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Leukocyte surface antigen CD47 (CD47) LDTP05572 6.92  LDD2028 
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49 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
60S ribosomal export protein NMD3 (NMD3) LDTP10200 7.11  LDD2028 
ADP-ribosylation factor-like protein 6-interacting protein 1 (ARL6IP1) LDTP06291 5.82  LDD2028 
Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) LDTP08364 5.17  LDD2028 
Apolipoprotein L2 (APOL2) LDTP11076 36.50  LDD2028 
Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) LDTP13755 6.96  LDD2028 
Centrosomal protein 43 (CEP43) LDTP01652 5.35  LDD2028 
Complex I assembly factor TIMMDC1, mitochondrial (TIMMDC1) LDTP12379 5.28  LDD2028 
CYFIP-related Rac1 interactor B (CYRIB) LDTP12642 6.59  LDD2028 
Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) LDTP13902 20.68  LDD2028 
DDRGK domain-containing protein 1 (DDRGK1) LDTP10403 6.92  LDD2028 
EF-hand calcium-binding domain-containing protein 14 (EFCAB14) LDTP19021 6.96  LDD2028 
Endoplasmic reticulum-Golgi intermediate compartment protein 2 (ERGIC2) LDTP10775 6.11  LDD2028 
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) LDTP13828 7.52  LDD2028 
ER lumen protein-retaining receptor 2 (KDELR2) LDTP03678 5.70  LDD2028 
FUN14 domain-containing protein 2 (FUNDC2) LDTP11326 22.16  LDD2028 
Golgi phosphoprotein 3 (GOLPH3) LDTP11911 5.35  LDD2028 
HEAT repeat-containing protein 3 (HEATR3) LDTP15253 4.92  LDD2028 
Inhibitor of nuclear factor kappa-B kinase-interacting protein (IKBIP) LDTP07891 8.11  LDD2028 
Kinetochore-associated protein 1 (KNTC1) LDTP04382 5.21  LDD2028 
Lamina-associated polypeptide 2, isoforms beta/gamma (TMPO) LDTP03968 6.41  LDD2028 
Large proline-rich protein BAG6 (BAG6) LDTP04094 5.43  LDD2028 
LYR motif-containing protein 2 (LYRM2) LDTP18467 6.32  LDD2028 
Mediator of RNA polymerase II transcription subunit 24 (MED24) LDTP01225 4.92  LDD2028 
Mitochondrial fission process protein 1 (MTFP1) LDTP16192 5.70  LDD2028 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 15.78  LDD2028 
Negative elongation factor C/D (NELFCD) LDTP08594 16.68  LDD2028 
Neuferricin (CYB5D2) LDTP15623 6.87  LDD2028 
Nucleobindin-1 (NUCB1) LDTP05375 6.96  LDD2028 
Nucleobindin-2 (NUCB2) LDTP05160 14.62  LDD2028 
OCIA domain-containing protein 1 (OCIAD1) LDTP12755 5.66  LDD2028 
Optic atrophy 3 protein (OPA3) LDTP16006 9.51  LDD2028 
Poly(U)-binding-splicing factor PUF60 (PUF60) LDTP13311 70.52  LDD2028 
Protein FAM114A2 (FAM114A2) LDTP16080 7.06  LDD2028 
Protein FAM162A (FAM162A) LDTP10056 10.20  LDD2028 
Protein YIF1A (YIF1A) LDTP01494 5.54  LDD2028 
Regulator of MON1-CCZ1 complex (RMC1) LDTP10218 5.13  LDD2028 
Reticulon-3 (RTN3) LDTP01522 32.67  LDD2028 
Reticulon-4 (RTN4) LDTP12412 8.88  LDD2028 
Signal recognition particle receptor subunit beta (SRPRB) LDTP16323 7.21  LDD2028 
Syntaxin-10 (STX10) LDTP00986 7.36  LDD2028 
Syntaxin-18 (STX18) LDTP13086 30.70  LDD2028 
TBC1 domain family member 9B (TBC1D9B) LDTP15057 7.31  LDD2028 
Testis-expressed protein 264 (TEX264) LDTP14259 5.21  LDD2028 
Tetratricopeptide repeat protein 19, mitochondrial (TTC19) LDTP07368 6.36  LDD2028 
Thioredoxin domain-containing protein 15 (TXNDC15) LDTP10442 5.21  LDD2028 
Thioredoxin-related transmembrane protein 4 (TMX4) LDTP18373 7.21  LDD2028 
TraB domain-containing protein (TRABD) LDTP19963 8.06  LDD2028 
Tudor and KH domain-containing protein (TDRKH) LDTP13922 7.01  LDD2028 
Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) LDTP15067 15.78  LDD2028 
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⏷ Show the Full List of 49 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD2028 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587