Details of the Target

General Information of Target

Target ID LDTP04956
Target Name Mitochondrial import inner membrane translocase subunit Tim10 (TIMM10)
Gene Name TIMM10
Gene ID 26519
Synonyms
TIM10; Mitochondrial import inner membrane translocase subunit Tim10
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDPLRAQQLAAELEVEMMADMYNRMTSACHRKCVPPHYKEAELSKGESVCLDRCVSKYLD
IHERMGKKLTELSMQDEELMKRVQQSSGPA
Target Bioclass
Transporter and channel
Family
Small Tim family
Subcellular location
Mitochondrion inner membrane
Function
Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.
Uniprot ID
P62072
Ensemble ID
ENST00000257245.9
HGNC ID
HGNC:11814

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C50(2.68)  LDD3310  [1]
BTD
 Probe Info  		C29(1.13)  LDD1700  [2]
IPM
 Probe Info  		C29(1.40)  LDD1701  [2]
Acrolein
 Probe Info  		N.A.  LDD0222  [3]
5E-2FA
 Probe Info  		N.A.  LDD2235  [4]
ATP probe
 Probe Info  		N.A.  LDD0199  [5]
m-APA
 Probe Info  		N.A.  LDD2231  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [6]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [6]
TFBX
 Probe Info  		C33(0.00); C29(0.00)  LDD0148  [7]
Crotonaldehyde
 Probe Info  		H62(0.00); H30(0.00)  LDD0219  [3]
Methacrolein
 Probe Info  		C29(0.00); C50(0.00)  LDD0218  [3]
NAIA_5
 Probe Info  		C33(0.00); C54(0.00); C50(0.00); C29(0.00)  LDD2223  [8]
PAL-AfBPP Probe
Click To Hide/Show 45 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C091
 Probe Info  		20.53  LDD1782  [9]
C092
 Probe Info  		27.86  LDD1783  [9]
C163
 Probe Info  		9.85  LDD1843  [9]
C165
 Probe Info  		19.97  LDD1845  [9]
C166
 Probe Info  		7.94  LDD1846  [9]
C177
 Probe Info  		11.71  LDD1856  [9]
C178
 Probe Info  		63.12  LDD1857  [9]
C229
 Probe Info  		8.82  LDD1902  [9]
C231
 Probe Info  		30.91  LDD1904  [9]
C232
 Probe Info  		99.73  LDD1905  [9]
C233
 Probe Info  		10.06  LDD1906  [9]
C234
 Probe Info  		16.68  LDD1907  [9]
C235
 Probe Info  		39.95  LDD1908  [9]
C243
 Probe Info  		32.22  LDD1916  [9]
C246
 Probe Info  		40.79  LDD1919  [9]
C249
 Probe Info  		31.56  LDD1922  [9]
C264
 Probe Info  		30.06  LDD1935  [9]
C265
 Probe Info  		33.59  LDD1936  [9]
C266
 Probe Info  		10.48  LDD1937  [9]
C269
 Probe Info  		8.94  LDD1939  [9]
C270
 Probe Info  		13.09  LDD1940  [9]
C273
 Probe Info  		4.92  LDD1943  [9]
C277
 Probe Info  		39.67  LDD1947  [9]
C285
 Probe Info  		60.55  LDD1955  [9]
C287
 Probe Info  		12.82  LDD1957  [9]
C288
 Probe Info  		9.58  LDD1958  [9]
C289
 Probe Info  		55.33  LDD1959  [9]
C290
 Probe Info  		5.28  LDD1960  [9]
C293
 Probe Info  		51.98  LDD1963  [9]
C296
 Probe Info  		43.41  LDD1966  [9]
C349
 Probe Info  		10.85  LDD2010  [9]
C350
 Probe Info  		22.01  LDD2011  [9]
C362
 Probe Info  		99.73  LDD2023  [9]
C363
 Probe Info  		33.13  LDD2024  [9]
C364
 Probe Info  		37.53  LDD2025  [9]
C366
 Probe Info  		8.46  LDD2027  [9]
C367
 Probe Info  		6.45  LDD2028  [9]
C429
 Probe Info  		41.64  LDD2084  [9]
C430
 Probe Info  		8.22  LDD2085  [9]
C431
 Probe Info  		99.73  LDD2086  [9]
C433
 Probe Info  		8.51  LDD2088  [9]
FFF probe11
 Probe Info  		20.00  LDD0471  [10]
FFF probe13
 Probe Info  		20.00  LDD0475  [10]
FFF probe14
 Probe Info  		20.00  LDD0477  [10]
JN0003
 Probe Info  		20.00  LDD0469  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C54(0.39)  LDD2142  [2]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C54(0.31)  LDD2112  [2]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C29(0.45)  LDD2117  [2]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C29(0.88)  LDD2152  [2]
 LDCM0520  AKOS000195272 MDA-MB-231 C29(0.85); C50(1.13); C54(0.56)  LDD2113  [2]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [3]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C29(0.97)  LDD1702  [2]
 LDCM0022  KB02 A101D C50(1.17)  LDD2250  [1]
 LDCM0023  KB03 MDA-MB-231 C29(1.40)  LDD1701  [2]
 LDCM0024  KB05 COLO792 C50(2.68)  LDD3310  [1]
 LDCM0109  NEM HeLa N.A.  LDD0226  [3]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C54(3.72)  LDD2092  [2]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C29(0.72); C54(0.60)  LDD2097  [2]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C54(0.15)  LDD2100  [2]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C29(1.49); C54(1.04)  LDD2101  [2]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C54(0.29)  LDD2104  [2]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C54(0.34)  LDD2106  [2]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C29(0.72)  LDD2107  [2]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C54(0.34)  LDD2108  [2]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C29(0.72)  LDD2109  [2]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C54(0.30)  LDD2110  [2]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C29(1.45)  LDD2111  [2]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C29(0.52); C54(0.64)  LDD2115  [2]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C29(0.36)  LDD2120  [2]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C29(0.94)  LDD2125  [2]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C29(0.41)  LDD2128  [2]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C29(0.68); C54(0.57)  LDD2133  [2]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C29(0.48); C50(0.91); C54(0.55)  LDD2134  [2]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C29(0.79)  LDD2136  [2]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C29(1.13)  LDD1700  [2]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C29(0.82)  LDD2140  [2]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C54(0.67)  LDD2141  [2]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C29(0.36)  LDD2143  [2]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C54(2.85)  LDD2145  [2]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C29(0.96)  LDD2146  [2]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C29(0.60)  LDD2147  [2]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C29(0.56); C50(0.79); C54(0.39)  LDD2148  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Putative serine/threonine-protein kinase SIK1B (SIK1B) CAMK Ser/Thr protein kinase family A0A0B4J2F2
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein 655 (ZNF655) Krueppel C2H2-type zinc-finger protein family Q8N720
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mitochondrial import inner membrane translocase subunit Tim9 (TIMM9) Small Tim family Q9Y5J7
TBC1 domain family member 21 (TBC1D21) . Q8IYX1

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
10 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.