Probe Name	C292					Probe Info
Probe concentration	Probe:50uM; negative probe:50uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	TMT
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP11273	N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1)					31.6
LDTP15422	Prostaglandin reductase 3 (PTGR3)					25.6
LDTP16007	Lipid droplet-associated hydrolase (LDAH)					25.5
LDTP09799	DCN1-like protein 4 (DCUN1D4)					18.5
LDTP04712	Puromycin-sensitive aminopeptidase (NPEPPS)					15.7
LDTP12293	Adipocyte plasma membrane-associated protein (APMAP)					8.9
LDTP02549	Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB)					8.6
LDTP03540	Heme oxygenase 2 (HMOX2)					8.6
LDTP05758	S-methyl-5'-thioadenosine phosphorylase (MTAP)					7.8
LDTP04689	Adenosine kinase (ADK)					7.7
LDTP04569	Geranylgeranyl transferase type-2 subunit beta (RABGGTB)					7.3
LDTP04253	Alpha-aminoadipic semialdehyde dehydrogenase (ALDH7A1)					7.2
LDTP09088	Phospholipase A2 group XV (PLA2G15)					7.1
LDTP12363	Lysophosphatidic acid phosphatase type 6 (ACP6)					7.0
LDTP06262	N-alpha-acetyltransferase 25, NatB auxiliary subunit (NAA25)					6.6
LDTP10032	7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B)					6.3
LDTP01641	STAM-binding protein (STAMBP)					6.1
LDTP17022	Secernin-3 (SCRN3)					6.1
LDTP15328	Dolichyldiphosphatase 1 (DOLPP1)					5.8
LDTP04285	Proteasome subunit beta type-2 (PSMB2)					5.7
LDTP03541	Adenylosuccinate synthetase isozyme 2 (ADSS2)					5.6
LDTP10676	Inositol polyphosphate-4-phosphatase type I A (INPP4A)					5.5
LDTP10495	Ubiquitin carboxyl-terminal hydrolase 47 (USP47)					5.5
LDTP06645	Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (HADH)					5.4
LDTP13282	Prenylcysteine oxidase 1 (PCYOX1)					5.3
LDTP02264	Asparagine synthetase [glutamine-hydrolyzing] (ASNS)					5.2
LDTP05476	3-ketodihydrosphingosine reductase (KDSR)					5.2
LDTP01982	NADH-ubiquinone oxidoreductase chain 1 (MT-ND1)					5.2
LDTP02175	Epoxide hydrolase 1 (EPHX1)					4.9
LDTP04884	Proteasome subunit alpha type-6 (PSMA6)					4.9

Target ID	Target Name					Binding Ratio
LDTP05900	Metaxin-1 (MTX1)					22.2
LDTP01059	Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B)					17.0
LDTP10913	Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A)					10.8
LDTP16069	Peroxisomal membrane protein 2 (PXMP2)					8.2
LDTP02724	Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2)					6.7
LDTP09547	Scavenger receptor class B member 1 (SCARB1)					6.5
LDTP07156	Protein wntless homolog (WLS)					5.6
LDTP04789	Synaptojanin-2-binding protein (SYNJ2BP)					5.4
LDTP00865	Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20)					5.3
LDTP15948	Transmembrane protein 126A (TMEM126A)					5.3
LDTP03277	ER lumen protein-retaining receptor 1 (KDELR1)					5.3
LDTP04033	Translocon-associated protein subunit alpha (SSR1)					5.2
LDTP08673	Calcium uptake protein 2, mitochondrial (MICU2)					5.0
LDTP11041	Calcium uptake protein 1, mitochondrial (MICU1)					4.9

Target ID	Target Name					Binding Ratio
LDTP12565	Mitochondrial import receptor subunit TOM22 homolog (TOMM22)					7.4
LDTP01075	Protein CutA (CUTA)					6.6
LDTP13828	Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3)					6.3
LDTP11076	Apolipoprotein L2 (APOL2)					6.2
LDTP02825	Histone H2AX (H2AX)					6.0
LDTP13630	Neudesin (NENF)					5.8
LDTP00105	Protein unc-119 homolog B (UNC119B)					5.5

1	Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26. Mass spectrometry data entry: PXD041587