Details of the Target

General Information of Target

Target ID LDTP12042
Target Name Proton-activated chloride channel (PACC1)
Gene Name PACC1
Gene ID 55248
Synonyms
C1orf75; TMEM206; Proton-activated chloride channel; PAC; hPAC; Acid-sensitive outwardly-rectifying anion channel; ASOR; Proton-activated outwardly rectifying anion channel; PAORAC; Transmembrane protein 206; hTMEM206
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTSRDQPRPKGPPKSTSPCPGISNSESSPTLNYQGILNRLKQFPRFSPHFAAELESIYYS
LHKIQQDVAEHHKQIGNVLQIVESCSQLQGFQSEEVSPAEPASPGTPQQVKDKTLQESSF
EDIMATRSSDWLRRPLGEDNQPETQLFWDKEPWFWHDTLTEQLWRIFAGVHDEKAKPRDR
QQAPGLGQESKAPGSCDPGTDPCPEDASTPRPPEASSSPPEGSQDRNTSWGVVQEPPGRA
SRFLQSISWDPEDFEDAWKRPDALPGQSKRLAVPCKLEKMRILAHGELVLATAISSFTRH
VFTCGRRGIKVWSLTGQVAEDRFPESHLPIQTPGAFLRTCLLSSNSRSLLTGGYNLASVS
VWDLAAPSLHVKEQLPCAGLNCQALDANLDANLAFASFTSGVVRIWDLRDQSVVRDLKGY
PDGVKSIVVKGYNIWTGGPDACLRCWDQRTIMKPLEYQFKSQIMSLSHSPQEDWVLLGMA
NGQQWLQSTSGSQRHMVGQKDSVILSVKFSPFGQWWASVGMDDFLGVYSMPAGTKVFEVP
EMSPVTCCDVSSNNRLVVTGSGEHASVYQITY
Target Bioclass
Transporter and channel
Family
Proton-activated chloride channel family
Subcellular location
Cell membrane
Function
Chloride channel gated by pH that facilitates the entry of chloride ions into cells upon exposure to extracellular acidic pH. Involved in acidosis-induced cell death by mediating chloride influx and subsequent cell swelling.
Uniprot ID
Q9H813
Ensemble ID
ENST00000261455.9
HGNC ID
HGNC:25593

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL51 Deletion: p.S277VfsTer22 .
MCC26 SNV: p.A320E .
MOLT4 Deletion: p.S277VfsTer22 IA-alkyne    Probe Info 
NCIH2286 SNV: p.P144A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info  		5.36  LDD0054  [1]
DBIA
 Probe Info  		C223(1.13)  LDD1508  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [3]
AOyne
 Probe Info  		14.80  LDD0443  [4]
PAL-AfBPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C170
 Probe Info  		26.17  LDD1850  [5]
C171
 Probe Info  		14.83  LDD1851  [5]
C281
 Probe Info  		23.75  LDD1951  [5]
C282
 Probe Info  		19.03  LDD1952  [5]
C310
 Probe Info  		20.68  LDD1977  [5]
FFF probe11
 Probe Info  		13.30  LDD0471  [6]
FFF probe12
 Probe Info  		17.88  LDD0473  [6]
FFF probe15
 Probe Info  		8.84  LDD0478  [6]
FFF probe2
 Probe Info  		16.41  LDD0463  [6]
FFF probe4
 Probe Info  		6.32  LDD0466  [6]
FFF probe6
 Probe Info  		8.66  LDD0467  [6]
FFF probe9
 Probe Info  		17.89  LDD0470  [6]
STS-1
 Probe Info  		N.A.  LDD0137  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C223(1.13)  LDD1508  [2]
 LDCM0226  AC11 HEK-293T C128(0.93)  LDD1509  [2]
 LDCM0259  AC14 HEK-293T C223(0.87)  LDD1512  [2]
 LDCM0277  AC18 HEK-293T C223(1.16)  LDD1516  [2]
 LDCM0278  AC19 HEK-293T C128(1.10)  LDD1517  [2]
 LDCM0279  AC2 HEK-293T C223(1.18)  LDD1518  [2]
 LDCM0282  AC22 HEK-293T C223(0.90)  LDD1521  [2]
 LDCM0286  AC26 HEK-293T C223(1.06)  LDD1525  [2]
 LDCM0287  AC27 HEK-293T C128(0.93)  LDD1526  [2]
 LDCM0290  AC3 HEK-293T C128(0.99)  LDD1529  [2]
 LDCM0291  AC30 HEK-293T C223(0.92)  LDD1530  [2]
 LDCM0295  AC34 HEK-293T C223(1.05)  LDD1534  [2]
 LDCM0296  AC35 HEK-293T C128(1.04)  LDD1535  [2]
 LDCM0299  AC38 HEK-293T C223(0.92)  LDD1538  [2]
 LDCM0304  AC42 HEK-293T C223(1.22)  LDD1543  [2]
 LDCM0305  AC43 HEK-293T C128(0.95)  LDD1544  [2]
 LDCM0308  AC46 HEK-293T C223(0.87)  LDD1547  [2]
 LDCM0313  AC50 HEK-293T C223(1.22)  LDD1552  [2]
 LDCM0314  AC51 HEK-293T C128(1.01)  LDD1553  [2]
 LDCM0317  AC54 HEK-293T C223(0.95)  LDD1556  [2]
 LDCM0321  AC58 HEK-293T C223(1.29)  LDD1560  [2]
 LDCM0322  AC59 HEK-293T C128(0.96)  LDD1561  [2]
 LDCM0323  AC6 HEK-293T C223(1.03)  LDD1562  [2]
 LDCM0326  AC62 HEK-293T C223(1.05)  LDD1565  [2]
 LDCM0368  CL10 HEK-293T C223(0.96)  LDD1572  [2]
 LDCM0371  CL102 HEK-293T C223(1.13); C128(1.17)  LDD1575  [2]
 LDCM0375  CL106 HEK-293T C223(0.94); C128(1.19)  LDD1579  [2]
 LDCM0380  CL110 HEK-293T C223(1.10); C128(1.12)  LDD1584  [2]
 LDCM0384  CL114 HEK-293T C223(1.13); C128(1.13)  LDD1588  [2]
 LDCM0388  CL118 HEK-293T C223(1.10); C128(1.16)  LDD1592  [2]
 LDCM0393  CL122 HEK-293T C223(1.07); C128(1.03)  LDD1597  [2]
 LDCM0397  CL126 HEK-293T C223(1.23); C128(1.14)  LDD1601  [2]
 LDCM0401  CL14 HEK-293T C223(1.08); C128(1.20)  LDD1605  [2]
 LDCM0405  CL18 HEK-293T C223(1.09)  LDD1609  [2]
 LDCM0406  CL19 HEK-293T C128(0.93)  LDD1610  [2]
 LDCM0407  CL2 HEK-293T C223(1.02); C128(1.06)  LDD1611  [2]
 LDCM0410  CL22 HEK-293T C223(1.01)  LDD1614  [2]
 LDCM0414  CL26 HEK-293T C223(0.83); C128(1.03)  LDD1618  [2]
 LDCM0419  CL30 HEK-293T C223(1.00)  LDD1623  [2]
 LDCM0420  CL31 HEK-293T C128(0.90)  LDD1624  [2]
 LDCM0423  CL34 HEK-293T C223(0.77)  LDD1627  [2]
 LDCM0432  CL42 HEK-293T C223(1.07)  LDD1636  [2]
 LDCM0433  CL43 HEK-293T C128(0.85)  LDD1637  [2]
 LDCM0436  CL46 HEK-293T C223(0.84)  LDD1640  [2]
 LDCM0441  CL50 HEK-293T C223(0.99); C128(1.18)  LDD1645  [2]
 LDCM0445  CL54 HEK-293T C223(0.96)  LDD1648  [2]
 LDCM0446  CL55 HEK-293T C128(0.92)  LDD1649  [2]
 LDCM0449  CL58 HEK-293T C223(0.92)  LDD1652  [2]
 LDCM0451  CL6 HEK-293T C223(1.22)  LDD1654  [2]
 LDCM0454  CL62 HEK-293T C223(0.92); C128(1.03)  LDD1657  [2]
 LDCM0458  CL66 HEK-293T C223(1.19)  LDD1661  [2]
 LDCM0459  CL67 HEK-293T C128(0.90)  LDD1662  [2]
 LDCM0462  CL7 HEK-293T C128(0.86)  LDD1665  [2]
 LDCM0463  CL70 HEK-293T C223(0.86)  LDD1666  [2]
 LDCM0467  CL74 HEK-293T C223(0.98); C128(1.12)  LDD1670  [2]
 LDCM0471  CL78 HEK-293T C223(1.10)  LDD1674  [2]
 LDCM0472  CL79 HEK-293T C128(0.88)  LDD1675  [2]
 LDCM0476  CL82 HEK-293T C223(0.85)  LDD1679  [2]
 LDCM0480  CL86 HEK-293T C223(1.11); C128(1.27)  LDD1683  [2]
 LDCM0485  CL90 HEK-293T C223(1.24)  LDD1688  [2]
 LDCM0486  CL91 HEK-293T C128(0.88)  LDD1689  [2]
 LDCM0489  CL94 HEK-293T C223(0.79)  LDD1692  [2]
 LDCM0493  CL98 HEK-293T C223(1.06); C128(1.03)  LDD1696  [2]
 LDCM0427  Fragment51 HEK-293T C223(0.90); C128(1.04)  LDD1631  [2]
 LDCM0008  Tranylcypromine SH-SY5Y 5.36  LDD0054  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
BOS complex subunit TMEM147 (TMEM147) TMEM147 family Q9BVK8
Proteolipid protein 2 (PLP2) . Q04941
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cortexin-3 (CTXN3) Cortexin family Q4LDR2
Plasmolipin (PLLP) MAL family Q9Y342
Vesicle-trafficking protein SEC22a (SEC22A) Synaptobrevin family Q96IW7

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
5 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
6 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
7 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.