Details of the Target

General Information of Target

Target ID LDTP00344
Target Name Stearoyl-CoA desaturase (SCD)
Gene Name SCD
Gene ID 6319
Synonyms
FADS5; SCD1; SCDOS; Stearoyl-CoA desaturase; hSCD1; EC 1.14.19.1; Acyl-CoA desaturase; Delta(9)-desaturase; Delta-9 desaturase; Fatty acid desaturase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPAHLLQDDISSSYTTTTTITAPPSRVLQNGGDKLETMPLYLEDDIRPDIKDDIYDPTYK
DKEGPSPKVEYVWRNIILMSLLHLGALYGITLIPTCKFYTWLWGVFYYFVSALGITAGAH
RLWSHRSYKARLPLRLFLIIANTMAFQNDVYEWARDHRAHHKFSETHADPHNSRRGFFFS
HVGWLLVRKHPAVKEKGSTLDLSDLEAEKLVMFQRRYYKPGLLMMCFILPTLVPWYFWGE
TFQNSVFVATFLRYAVVLNATWLVNSAAHLFGYRPYDKNISPRENILVSLGAVGEGFHNY
HHSFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAAILARIKRTGDGNYKSG
Target Type
Successful
Target Bioclass
Enzyme
Family
Fatty acid desaturase type 1 family
Subcellular location
Endoplasmic reticulum membrane
Function
Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids. Plays an important role in lipid biosynthesis. Plays an important role in regulating the expression of genes that are involved in lipogenesis and in regulating mitochondrial fatty acid oxidation. Plays an important role in body energy homeostasis. Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides.
TTD ID
T10897
Uniprot ID
O00767
DrugMap ID
TT6RIOV
Ensemble ID
ENST00000370355.3
HGNC ID
HGNC:10571
ChEMBL ID
CHEMBL5555

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Alkylaryl probe 2
 Probe Info  		20.00  LDD0389  [1]
TH211
 Probe Info  		Y356(20.00)  LDD0260  [2]
C-Sul
 Probe Info  		6.61  LDD0066  [3]
YN-4
 Probe Info  		100.00  LDD0445  [4]
STPyne
 Probe Info  		K189(1.85); K194(6.48); K341(5.06)  LDD0277  [5]
m-APA
 Probe Info  		11.79  LDD0403  [6]
Alkylaryl probe 3
 Probe Info  		20.00  LDD0282  [7]
HPAP
 Probe Info  		4.05  LDD0062  [8]
Acrolein
 Probe Info  		N.A.  LDD0221  [9]
SF
 Probe Info  		N.A.  LDD0028  [10]
Crotonaldehyde
 Probe Info  		H171(0.00); H167(0.00)  LDD0219  [9]
AOyne
 Probe Info  		10.80  LDD0443  [11]
PAL-AfBPP Probe
Click To Hide/Show 23 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C027
 Probe Info  		7.11  LDD1733  [12]
C040
 Probe Info  		14.22  LDD1740  [12]
C092
 Probe Info  		32.90  LDD1783  [12]
C094
 Probe Info  		25.81  LDD1785  [12]
C112
 Probe Info  		19.16  LDD1799  [12]
C166
 Probe Info  		7.84  LDD1846  [12]
C218
 Probe Info  		14.42  LDD1892  [12]
C219
 Probe Info  		4.92  LDD1893  [12]
C220
 Probe Info  		14.32  LDD1894  [12]
C231
 Probe Info  		13.36  LDD1904  [12]
C235
 Probe Info  		25.46  LDD1908  [12]
C289
 Probe Info  		44.02  LDD1959  [12]
C349
 Probe Info  		9.99  LDD2010  [12]
C350
 Probe Info  		23.92  LDD2011  [12]
C362
 Probe Info  		38.05  LDD2023  [12]
C366
 Probe Info  		7.01  LDD2027  [12]
C407
 Probe Info  		13.93  LDD2064  [12]
FFF probe11
 Probe Info  		20.00  LDD0472  [13]
FFF probe15
 Probe Info  		20.00  LDD0478  [13]
FFF probe3
 Probe Info  		15.71  LDD0465  [13]
STS-2
 Probe Info  		N.A.  LDD0138  [14]
VE-P
 Probe Info  		N.A.  LDD0396  [15]
OEA-DA
 Probe Info  		5.77  LDD0046  [16]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.79  LDD0403  [6]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [9]
 LDCM0107  IAA HeLa N.A.  LDD0221  [9]
 LDCM0109  NEM HeLa N.A.  LDD0223  [9]
 LDCM0014  Panhematin HEK-293T 4.05  LDD0062  [8]
 LDCM0099  Phenelzine HEK-293T 20.00  LDD0282  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Probable glutathione peroxidase 8 (GPX8) Glutathione peroxidase family Q8TED1
Glutathione S-transferase 3, mitochondrial (MGST3) MAPEG family O14880
Signal peptidase complex catalytic subunit SEC11C (SEC11C) Peptidase S26B family Q9BY50
E3 ubiquitin-protein ligase RNF5 (RNF5) RNF5 family Q99942
17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13) Short-chain dehydrogenases/reductases (SDR) family Q7Z5P4
TLC domain-containing protein 4 (TLCD4) TLCD4 family Q96MV1
Ceramide synthase 4 (CERS4) . Q9HA82
E3 ubiquitin-protein ligase MARCHF5 (MARCHF5) . Q9NX47
Transmembrane ascorbate-dependent reductase CYB561 (CYB561) . P49447
Transporter and channel
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Solute carrier family 7 member 14 (SLC7A14) Cationic amino acid transporter (CAT) (TC 2.A.3.3) family Q8TBB6
Stomatin (STOM) Band 7/mec-2 family P27105
Sodium-dependent organic anion transporter (SLC10A6) Bile acid:sodium symporter (BASS) family Q3KNW5
Cell cycle control protein 50B (TMEM30B) CDC50/LEM3 family Q3MIR4
Gap junction beta-1 protein (GJB1) Connexin family P08034
G protein-activated inward rectifier potassium channel 2 (KCNJ6) Inward rectifier-type potassium channel family P48051
Hippocampus abundant transcript-like protein 1 (MFSD14B) Major facilitator superfamily Q5SR56
Aquaporin-6 (AQP6) MIP/aquaporin (TC 1.A.8) family Q13520
Sodium channel regulatory subunit beta-3 (SCN3B) Sodium channel auxiliary subunit SCN3B family Q9NY72
Multiple coagulation factor deficiency protein 2 (MCFD2) . Q8NI22
Thioredoxin-related transmembrane protein 2 (TMX2) . Q9Y320
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) BZIP family Q96BA8
GPCR
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
G-protein coupled receptor 42 (GPR42) G-protein coupled receptor 1 family O15529
Probable G-protein coupled receptor 152 (GPR152) G-protein coupled receptor 1 family Q8TDT2
Immunoglobulin
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Myeloid cell surface antigen CD33 (CD33) SIGLEC (sialic acid binding Ig-like lectin) family P20138
Poliovirus receptor (PVR) Nectin family P15151
Other
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytochrome b-245 chaperone 1 (CYBC1) CYBC1 family Q9BQA9
Receptor expression-enhancing protein 2 (REEP2) DP1 family Q9BRK0
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) ERGIC family Q9Y282
Translation initiation factor IF-3, mitochondrial (MTIF3) IF-3 family Q9H2K0
Protein jagunal homolog 1 (JAGN1) Jagunal family Q8N5M9
Reticulophagy regulator 3 (RETREG3) RETREG family Q86VR2
Complex I assembly factor TIMMDC1, mitochondrial (TIMMDC1) Tim17/Tim22/Tim23 family Q9NPL8
Vacuolar ATPase assembly integral membrane protein VMA21 (VMA21) VMA21 family Q3ZAQ7
C-type lectin domain family 4 member K (CD207) . Q9UJ71
Sperm-associated antigen 4 protein (SPAG4) . Q9NPE6

References

1 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
4 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
5 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
6 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
7 Activity-Based Hydrazine Probes for Protein Profiling of Electrophilic Functionality in Therapeutic Targets. ACS Cent Sci. 2021 Sep 22;7(9):1524-1534. doi: 10.1021/acscentsci.1c00616. Epub 2021 Aug 19.
8 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
9 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
10 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
11 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
12 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
13 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
14 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
15 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
16 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570