Details of the Target

General Information of Target

Target ID LDTP00199
Target Name HCLS1-associated protein X-1 (HAX1)
Gene Name HAX1
Gene ID 10456
Synonyms
HS1BP1; HCLS1-associated protein X-1; HS1-associating protein X-1; HAX-1; HS1-binding protein 1; HSP1BP-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSLFDLFRGFFGFPGPRSHRDPFFGGMTRDEDDDEEEEEEGGSWGRGNPRFHSPQHPPEE
FGFGFSFSPGGGIRFHDNFGFDDLVRDFNSIFSDMGAWTLPSHPPELPGPESETPGERLR
EGQTLRDSMLKYPDSHQPRIFGGVLESDARSESPQPAPDWGSQRPFHRFDDVWPMDPHPR
TREDNDLDSQVSQEGLGPVLQPQPKSYFKSISVTKITKPDGIVEERRTVVDSEGRTETTV
TRHEADSSPRGDPESPRPPALDDAFSILDLFLGRWFRSR
Target Type
Literature-reported
Target Bioclass
Other
Family
HAX1 family
Subcellular location
Cytoplasm; Mitochondrion matrix
Function
Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex. Slows down the rate of inactivation of KCNC3 channels. Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.
TTD ID
T20256
Uniprot ID
O00165
DrugMap ID
TT21BYA
Ensemble ID
ENST00000328703.12
HGNC ID
HGNC:16915
ChEMBL ID
CHEMBL4295644

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A3KAW SNV: p.D83N .
HCT116 Deletion: p.F24LfsTer4 .
JURKAT SNV: p.R274C .
MCC13 SNV: p.P14S .
PC9 SNV: p.P198Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K209(4.98)  LDD0277  [1]
Acrolein
 Probe Info  		N.A.  LDD0221  [2]
m-APA
 Probe Info  		N.A.  LDD2231  [3]
AOyne
 Probe Info  		12.80  LDD0443  [4]
PAL-AfBPP Probe
Click To Hide/Show 41 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C022
 Probe Info  		7.21  LDD1728  [5]
C026
 Probe Info  		9.78  LDD1732  [5]
C055
 Probe Info  		13.00  LDD1752  [5]
C056
 Probe Info  		17.15  LDD1753  [5]
C064
 Probe Info  		6.45  LDD1761  [5]
C112
 Probe Info  		37.27  LDD1799  [5]
C186
 Probe Info  		8.00  LDD1864  [5]
C187
 Probe Info  		20.11  LDD1865  [5]
C191
 Probe Info  		12.30  LDD1868  [5]
C201
 Probe Info  		51.63  LDD1877  [5]
C206
 Probe Info  		23.43  LDD1881  [5]
C232
 Probe Info  		58.49  LDD1905  [5]
C233
 Probe Info  		16.34  LDD1906  [5]
C235
 Probe Info  		16.80  LDD1908  [5]
C238
 Probe Info  		14.12  LDD1911  [5]
C243
 Probe Info  		11.16  LDD1916  [5]
C244
 Probe Info  		20.39  LDD1917  [5]
C245
 Probe Info  		10.48  LDD1918  [5]
C246
 Probe Info  		16.45  LDD1919  [5]
C249
 Probe Info  		18.90  LDD1922  [5]
C289
 Probe Info  		30.48  LDD1959  [5]
C338
 Probe Info  		11.00  LDD2001  [5]
C350
 Probe Info  		35.02  LDD2011  [5]
C355
 Probe Info  		36.76  LDD2016  [5]
C356
 Probe Info  		15.03  LDD2017  [5]
C360
 Probe Info  		5.43  LDD2021  [5]
C361
 Probe Info  		18.00  LDD2022  [5]
C362
 Probe Info  		37.53  LDD2023  [5]
C363
 Probe Info  		15.67  LDD2024  [5]
C364
 Probe Info  		17.03  LDD2025  [5]
C380
 Probe Info  		10.48  LDD2039  [5]
C382
 Probe Info  		9.99  LDD2041  [5]
C388
 Probe Info  		56.49  LDD2047  [5]
FFF probe11
 Probe Info  		20.00  LDD0471  [6]
FFF probe13
 Probe Info  		20.00  LDD0475  [6]
FFF probe14
 Probe Info  		16.79  LDD0477  [6]
FFF probe15
 Probe Info  		10.96  LDD0478  [6]
FFF probe2
 Probe Info  		20.00  LDD0463  [6]
FFF probe3
 Probe Info  		20.00  LDD0464  [6]
FFF probe4
 Probe Info  		19.88  LDD0466  [6]
JN0003
 Probe Info  		20.00  LDD0469  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [2]
 LDCM0107  IAA HeLa N.A.  LDD0221  [2]
 LDCM0109  NEM HeLa H136(0.00); H76(0.00)  LDD0223  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone acetyltransferase KAT5 (KAT5) MYST (SAS/MOZ) family Q92993
Transporter and channel
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein gamma (YWHAG) 14-3-3 family P61981
Two pore channel protein 1 (TPCN1) Calcium channel alpha-1 subunit (TC 1.A.1.11) family Q9ULQ1
Two pore channel protein 2 (TPCN2) Calcium channel alpha-1 subunit (TC 1.A.1.11) family Q8NHX9
Huntingtin (HTT) Huntingtin family P42858
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Estrogen receptor (ESR1) Nuclear hormone receptor family P03372
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Interleukin-1 alpha (IL1A) IL-1 family P01583
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein pelota homolog (PELO) Eukaryotic release factor 1 family Q9BRX2
Growth factor receptor-bound protein 7 (GRB7) GRB7/10/14 family Q14451
Protein salvador homolog 1 (SAV1) . Q9H4B6

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
5 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
6 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.