Details of the Target

General Information of Target

Target ID LDTP02756
Target Name Junction plakoglobin (JUP)
Gene Name JUP
Gene ID 3728
Synonyms
CTNNG; DP3; Junction plakoglobin; Catenin gamma; Desmoplakin III; Desmoplakin-3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEVMNLMEQPIKVTEWQQTYTYDSGIHSGANTCVPSVSSKGIMEEDEACGRQYTLKKTTT
YTQGVPPSQGDLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQATNLQRLAEPS
QLLKSAIVHLINYQDDAELATRALPELTKLLNDEDPVVVTKAAMIVNQLSKKEASRRALM
GSPQLVAAVVRTMQNTSDLDTARCTTSILHNLSHHREGLLAIFKSGGIPALVRMLSSPVE
SVLFYAITTLHNLLLYQEGAKMAVRLADGLQKMVPLLNKNNPKFLAITTDCLQLLAYGNQ
ESKLIILANGGPQALVQIMRNYSYEKLLWTTSRVLKVLSVCPSNKPAIVEAGGMQALGKH
LTSNSPRLVQNCLWTLRNLSDVATKQEGLESVLKILVNQLSVDDVNVLTCATGTLSNLTC
NNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNY
GIPAIVKLLNQPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQR
HVAAGTQQPYTDGVRMEEIVEGCTGALHILARDPMNRMEIFRLNTIPLFVQLLYSSVENI
QRVAAGVLCELAQDKEAADAIDAEGASAPLMELLHSRNEGTATYAAAVLFRISEDKNPDY
RKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYGDDMDATYRPMYSSDVPLDPLEMHMDM
DGDYPIDTYSDGLRPPYPTADHMLA
Target Type
Literature-reported
Target Bioclass
Other
Family
Beta-catenin family
Subcellular location
Cell junction, adherens junction
Function
Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
TTD ID
T82685
Uniprot ID
P14923
DrugMap ID
TTREN0G
Ensemble ID
ENST00000310706.9
HGNC ID
HGNC:6207

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 37 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P3
 Probe Info  		2.29  LDD0450  [1]
P8
 Probe Info  		2.66  LDD0451  [1]
YN-1
 Probe Info  		100.00  LDD0444  [2]
Nap-2
 Probe Info  		N.A.  LDD0435  [3]
OPA-S-S-alkyne
 Probe Info  		K656(0.97)  LDD3494  [4]
DBIA
 Probe Info  		C381(2.78); C573(1.53); C511(1.24); C341(1.31)  LDD3310  [5]
BTD
 Probe Info  		C609(1.08)  LDD2106  [6]
Jackson_14
 Probe Info  		2.63  LDD0123  [7]
AHL-Pu-1
 Probe Info  		C511(2.00)  LDD0168  [8]
5E-2FA
 Probe Info  		H515(0.00); H129(0.00)  LDD2235  [9]
m-APA
 Probe Info  		H515(0.00); H129(0.00)  LDD2231  [9]
N1
 Probe Info  		C563(0.00); E561(0.00); H568(0.00)  LDD0245  [10]
IA-alkyne
 Probe Info  		C372(0.00); C457(0.00)  LDD0032  [11]
Lodoacetamide azide
 Probe Info  		C372(0.00); C563(0.00)  LDD0037  [12]
ATP probe
 Probe Info  		K426(0.00); K124(0.00)  LDD0035  [13]
IPM
 Probe Info  		C49(0.00); C90(0.00); C511(0.00); C457(0.00)  LDD0025  [14]
JW-RF-010
 Probe Info  		C511(0.00); C457(0.00)  LDD0026  [14]
NAIA_4
 Probe Info  		N.A.  LDD2226  [15]
TFBX
 Probe Info  		C341(0.00); C511(0.00); C90(0.00)  LDD0027  [14]
WYneN
 Probe Info  		N.A.  LDD0021  [16]
WYneO
 Probe Info  		N.A.  LDD0022  [16]
DA-P3
 Probe Info  		N.A.  LDD0178  [17]
1d-yne
 Probe Info  		N.A.  LDD0356  [18]
ENE
 Probe Info  		N.A.  LDD0006  [16]
NHS
 Probe Info  		N.A.  LDD0010  [16]
PF-06672131
 Probe Info  		C511(0.00); C609(0.00)  LDD0017  [19]
SF
 Probe Info  		N.A.  LDD0028  [20]
VSF
 Probe Info  		C341(0.00); C90(0.00)  LDD0007  [16]
Phosphinate-6
 Probe Info  		C511(0.00); C90(0.00); C341(0.00); C49(0.00)  LDD0018  [21]
1c-yne
 Probe Info  		K161(0.00); K124(0.00)  LDD0228  [18]
Acrolein
 Probe Info  		C511(0.00); C49(0.00); C33(0.00); H541(0.00)  LDD0217  [22]
Crotonaldehyde
 Probe Info  		C49(0.00); H360(0.00)  LDD0219  [22]
Methacrolein
 Probe Info  		C511(0.00); C33(0.00); C49(0.00); C457(0.00)  LDD0218  [22]
W1
 Probe Info  		C372(0.00); C609(0.00); C457(0.00); C511(0.00)  LDD0236  [23]
AOyne
 Probe Info  		7.80  LDD0443  [24]
NAIA_5
 Probe Info  		C457(0.00); C204(0.00); C291(0.00); C49(0.00)  LDD2223  [15]
HHS-465
 Probe Info  		N.A.  LDD2240  [25]
PAL-AfBPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		12.70  LDD0471  [26]
FFF probe12
 Probe Info  		8.54  LDD0473  [26]
FFF probe13
 Probe Info  		17.95  LDD0475  [26]
FFF probe15
 Probe Info  		5.88  LDD0478  [26]
FFF probe2
 Probe Info  		20.00  LDD0479  [26]
FFF probe3
 Probe Info  		20.00  LDD0464  [26]
FFF probe6
 Probe Info  		12.44  LDD0482  [26]
FFF probe9
 Probe Info  		12.71  LDD0470  [26]
STS-1
 Probe Info  		1.09  LDD0136  [27]
STS-2
 Probe Info  		1.27  LDD0138  [27]
SAHA-CA-8PAP
 Probe Info  		5.71  LDD0362  [28]
DA-2
 Probe Info  		N.A.  LDD0071  [29]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C511(2.00)  LDD0168  [8]
 LDCM0026  4SU-RNA+native RNA HEK-293T C511(2.02); C33(2.56)  LDD0169  [8]
 LDCM0108  Chloroacetamide HeLa C511(0.00); C49(0.00); H360(0.00); H541(0.00)  LDD0222  [22]
 LDCM0632  CL-Sc Hep-G2 C372(1.70); C511(0.80); C204(0.43); C90(0.40)  LDD2227  [15]
 LDCM0189  Compound 16 HEK-293T 71.43  LDD0492  [26]
 LDCM0182  Compound 18 HEK-293T 3.99  LDD0501  [26]
 LDCM0191  Compound 21 HEK-293T 8.58  LDD0493  [26]
 LDCM0190  Compound 34 HEK-293T 125.00  LDD0497  [26]
 LDCM0192  Compound 35 HEK-293T 7.66  LDD0491  [26]
 LDCM0193  Compound 36 HEK-293T 6.19  LDD0511  [26]
 LDCM0195  Compound 38 HEK-293T 14.71  LDD0499  [26]
 LDCM0197  Compound 40 HEK-293T 7.69  LDD0495  [26]
 LDCM0634  CY-0357 Hep-G2 C49(1.29); C511(0.57)  LDD2228  [15]
 LDCM0107  IAA HeLa H360(0.00); H515(0.00); H129(0.00)  LDD0221  [22]
 LDCM0022  KB02 22RV1 C90(1.23); C49(1.65); C381(2.64); C573(1.61)  LDD2243  [5]
 LDCM0023  KB03 22RV1 C90(1.30); C49(1.82); C381(5.47); C573(2.73)  LDD2660  [5]
 LDCM0024  KB05 COLO792 C381(2.78); C573(1.53); C511(1.24); C341(1.31)  LDD3310  [5]
 LDCM0109  NEM HeLa H466(0.00); H541(0.00); H360(0.00); H440(0.00)  LDD0223  [22]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C609(1.08)  LDD2106  [6]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C457(0.93)  LDD2125  [6]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C457(1.23); C511(0.73)  LDD2136  [6]
 LDCM0016  Ranjitkar_cp1 MDA-MB-231 2.63  LDD0123  [7]
 LDCM0096  SAHA K562 5.71  LDD0362  [28]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Probable ATP-dependent RNA helicase DDX6 (DDX6) DEAD box helicase family P26196
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Epidermal growth factor receptor (EGFR) Tyr protein kinase family P00533
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Platelet endothelial cell adhesion molecule (PECAM1) . P16284
Other
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Adenomatous polyposis coli protein (APC) Adenomatous polyposis coli (APC) family P25054
Beta-catenin-interacting protein 1 (CTNNBIP1) CTNNBIP1 family Q9NSA3
Radial spoke head 14 homolog (RSPH14) Flagellar radial spoke RSP14 family Q9UHP6
Sarcoplasmic reticulum histidine-rich calcium-binding protein (HRC) HRC family P23327
Axin-1 (AXIN1) . O15169
Cadherin-2 (CDH2) . P19022
Desmoglein-1 (DSG1) . Q02413
Four and a half LIM domains protein 2 (FHL2) . Q14192
Proline-rich protein 35 (PRR35) . P0CG20

The Drug(s) Related To This Target

Approved
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Zinc . DB01593
Zinc Acetate . DB14487

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 Proteome-Wide Identification of On- and Off-Targets of Bcl-2 Inhibitors in Native Biological Systems by Using Affinity-Based Probes (AfBPs). Chembiochem. 2018 Nov 2;19(21):2312-2320. doi: 10.1002/cbic.201800380. Epub 2018 Oct 18.
4 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
7 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
8 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
9 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
10 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
11 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
12 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
13 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
14 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
15 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
16 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
17 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
18 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
19 Site-Specific Activity-Based Protein Profiling Using Phosphonate Handles. Mol Cell Proteomics. 2023 Jan;22(1):100455. doi: 10.1016/j.mcpro.2022.100455. Epub 2022 Nov 24.
Mass spectrometry data entry: PXD036569
20 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
21 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
22 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
23 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
24 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
25 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
26 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
27 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
28 Streamlined Target Deconvolution Approach Utilizing a Single Photoreactive Chloroalkane Capture Tag. ACS Chem Biol. 2021 Feb 19;16(2):404-413. doi: 10.1021/acschembio.0c00987. Epub 2021 Feb 5.
29 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.