Details of the Target

General Information of Target

Target ID LDTP12468
Target Name Diablo IAP-binding mitochondrial protein (DIABLO)
Gene Name DIABLO
Gene ID 56616
Synonyms
SMAC; Diablo IAP-binding mitochondrial protein; Diablo homolog, mitochondrial; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; Smac) [Cleaved into: Diablo IAP-binding mitochondrial protein, cleaved form]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLRFLPDLAFSFLLILALGQAVQFQEYVFLQFLGLDKAPSPQKFQPVPYILKKIFQDREA
AATTGVSRDLCYVKELGVRGNVLRFLPDQGFFLYPKKISQASSCLQKLLYFNLSAIKERE
QLTLAQLGLDLGPNSYYNLGPELELALFLVQEPHVWGQTTPKPGKMFVLRSVPWPQGAVH
FNLLDVAKDWNDNPRKNFGLFLEILVKEDRDSGVNFQPEDTCARLRCSLHASLLVVTLNP
DQCHPSRKRRAAIPVPKLSCKNLCHRHQLFINFRDLGWHKWIIAPKGFMANYCHGECPFS
LTISLNSSNYAFMQALMHAVDPEIPQAVCIPTKLSPISMLYQDNNDNVILRHYEDMVVDE
CGCG
Target Type
Literature-reported
Target Bioclass
Other
Subcellular location
Mitochondrion
Function
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases.; [Isoform 3]: Attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation.; [Isoform 1]: Defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
TTD ID
T78150
Uniprot ID
Q9NR28
DrugMap ID
TTN74LE
Ensemble ID
ENST00000353548.11
HGNC ID
HGNC:21528

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
LNCaP clone FGC SNV: p.S126L .
MEWO SNV: p.S112F .
MFE319 SNV: p.S70G .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info  		7.80  LDD0318  [1]
OPA-S-S-alkyne
 Probe Info  		K219(1.92)  LDD3494  [2]
Probe 1
 Probe Info  		Y108(15.21); Y114(34.76)  LDD3495  [3]
5E-2FA
 Probe Info  		H147(0.00); H198(0.00); H66(0.00)  LDD2235  [4]
m-APA
 Probe Info  		H147(0.00); H198(0.00); H66(0.00)  LDD2231  [4]
NAIA_4
 Probe Info  		C23(0.00); C21(0.00)  LDD2226  [5]
NHS
 Probe Info  		N.A.  LDD0010  [6]
SF
 Probe Info  		N.A.  LDD0028  [7]
STPyne
 Probe Info  		N.A.  LDD0009  [6]
Acrolein
 Probe Info  		N.A.  LDD0217  [8]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [8]
AOyne
 Probe Info  		6.10  LDD0443  [9]
HHS-465
 Probe Info  		N.A.  LDD2240  [10]
PAL-AfBPP Probe
Click To Hide/Show 67 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C040
 Probe Info  		12.55  LDD1740  [11]
C041
 Probe Info  		9.38  LDD1741  [11]
C056
 Probe Info  		17.27  LDD1753  [11]
C063
 Probe Info  		10.63  LDD1760  [11]
C106
 Probe Info  		37.01  LDD1793  [11]
C107
 Probe Info  		5.54  LDD1794  [11]
C108
 Probe Info  		13.55  LDD1795  [11]
C112
 Probe Info  		20.68  LDD1799  [11]
C134
 Probe Info  		22.01  LDD1816  [11]
C153
 Probe Info  		21.26  LDD1834  [11]
C158
 Probe Info  		9.71  LDD1838  [11]
C159
 Probe Info  		9.25  LDD1839  [11]
C160
 Probe Info  		11.55  LDD1840  [11]
C161
 Probe Info  		14.32  LDD1841  [11]
C187
 Probe Info  		71.51  LDD1865  [11]
C191
 Probe Info  		44.02  LDD1868  [11]
C193
 Probe Info  		12.47  LDD1869  [11]
C210
 Probe Info  		61.82  LDD1884  [11]
C218
 Probe Info  		10.70  LDD1892  [11]
C220
 Probe Info  		17.63  LDD1894  [11]
C232
 Probe Info  		62.25  LDD1905  [11]
C238
 Probe Info  		21.11  LDD1911  [11]
C239
 Probe Info  		6.96  LDD1912  [11]
C240
 Probe Info  		9.51  LDD1913  [11]
C244
 Probe Info  		18.00  LDD1917  [11]
C249
 Probe Info  		13.83  LDD1922  [11]
C265
 Probe Info  		13.74  LDD1936  [11]
C277
 Probe Info  		13.55  LDD1947  [11]
C284
 Probe Info  		23.10  LDD1954  [11]
C293
 Probe Info  		18.25  LDD1963  [11]
C313
 Probe Info  		18.64  LDD1980  [11]
C314
 Probe Info  		13.36  LDD1981  [11]
C317
 Probe Info  		7.11  LDD1983  [11]
C320
 Probe Info  		5.86  LDD1986  [11]
C322
 Probe Info  		7.46  LDD1988  [11]
C326
 Probe Info  		11.39  LDD1990  [11]
C338
 Probe Info  		23.59  LDD2001  [11]
C343
 Probe Info  		16.22  LDD2005  [11]
C346
 Probe Info  		13.00  LDD2007  [11]
C348
 Probe Info  		10.85  LDD2009  [11]
C349
 Probe Info  		10.93  LDD2010  [11]
C350
 Probe Info  		45.57  LDD2011  [11]
C353
 Probe Info  		9.45  LDD2014  [11]
C354
 Probe Info  		8.06  LDD2015  [11]
C355
 Probe Info  		23.43  LDD2016  [11]
C361
 Probe Info  		23.75  LDD2022  [11]
C362
 Probe Info  		27.47  LDD2023  [11]
C363
 Probe Info  		25.28  LDD2024  [11]
C364
 Probe Info  		14.83  LDD2025  [11]
C376
 Probe Info  		6.92  LDD2036  [11]
C383
 Probe Info  		14.22  LDD2042  [11]
C386
 Probe Info  		8.06  LDD2045  [11]
C387
 Probe Info  		6.32  LDD2046  [11]
C388
 Probe Info  		49.87  LDD2047  [11]
C390
 Probe Info  		32.67  LDD2049  [11]
C391
 Probe Info  		16.45  LDD2050  [11]
C397
 Probe Info  		11.39  LDD2056  [11]
C399
 Probe Info  		7.62  LDD2058  [11]
C403
 Probe Info  		17.51  LDD2061  [11]
C429
 Probe Info  		15.14  LDD2084  [11]
C431
 Probe Info  		23.92  LDD2086  [11]
C433
 Probe Info  		5.86  LDD2088  [11]
FFF probe11
 Probe Info  		7.72  LDD0472  [12]
FFF probe13
 Probe Info  		19.21  LDD0475  [12]
FFF probe14
 Probe Info  		20.00  LDD0477  [12]
FFF probe2
 Probe Info  		20.00  LDD0463  [12]
BD-F
 Probe Info  		M158(0.00); E154(0.00); T159(0.00); T155(0.00)  LDD0024  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [8]
 LDCM0107  IAA HeLa H198(0.00); H186(0.00)  LDD0221  [8]
 LDCM0109  NEM HeLa H198(0.00); H186(0.00)  LDD0223  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Diacylglycerol O-acyltransferase 2-like protein 6 (DGAT2L6) Diacylglycerol acyltransferase family Q6ZPD8
Peroxisomal bifunctional enzyme (EHHADH) Enoyl-CoA hydratase/isomerase family; 3-hydroxyacyl-CoA dehydrogenase family Q08426
Fatty acid desaturase 6 (FADS6) Fatty acid desaturase type 1 family Q8N9I5
Baculoviral IAP repeat-containing protein 2 (BIRC2) IAP family Q13490
Baculoviral IAP repeat-containing protein 7 (BIRC7) IAP family Q96CA5
E3 ubiquitin-protein ligase XIAP (XIAP) IAP family P98170
Protein kinase C delta type (PRKCD) AGC Ser/Thr protein kinase family Q05655
17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13) Short-chain dehydrogenases/reductases (SDR) family Q7Z5P4
Dehydrogenase/reductase SDR family member 1 (DHRS1) Short-chain dehydrogenases/reductases (SDR) family Q96LJ7
V-type proton ATPase 16 kDa proteolipid subunit c (ATP6V0C) V-ATPase proteolipid subunit family P27449
Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) . Q9Y680
Transporter and channel
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Derlin-2 (DERL2) Derlin family Q9GZP9
Voltage-gated purine nucleotide uniporter SLC17A9 (SLC17A9) Sodium/anion cotransporter family Q9BYT1
Mitochondrial pyruvate carrier 2 (MPC2) Mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family O95563
Synaptophysin (SYP) Synaptophysin/synaptobrevin family P08247
Solute carrier family 66 member 2 (SLC66A2) . Q8N2U9
Other
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Apolipoprotein C-IV (APOC4) Apolipoprotein C4 family P55056
ADP-ribosylation factor-like protein 6-interacting protein 1 (ARL6IP1) ARL6ip family Q15041
Baculoviral IAP repeat-containing protein 5 (BIRC5) IAP family O15392
MICOS complex subunit MIC13 (MICOS13) MICOS complex subunit Mic13 family Q5XKP0
Mitochondrial fission regulator 1 (MTFR1) MTFR1 family Q15390
Regulator of microtubule dynamics protein 2 (RMDN2) RMDN family Q96LZ7
Vesicle transport protein SFT2A (SFT2D1) SFT2 family Q8WV19
Vesicle-trafficking protein SEC22a (SEC22A) Synaptobrevin family Q96IW7
Complex I assembly factor TIMMDC1, mitochondrial (TIMMDC1) Tim17/Tim22/Tim23 family Q9NPL8

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Phenethyl Isothiocyanate Small molecular drug DB12695

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
10 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
11 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
12 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
13 Evaluation of fully-functionalized diazirine tags for chemical proteomic applications. Chem Sci. 2021 May 7;12(22):7839-7847. doi: 10.1039/d1sc01360b.
Mass spectrometry data entry: PXD025652