Probe Name	C239					Probe Info
Probe concentration	Probe:50uM; negative probe:50uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	TMT
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP08476	5'-3' exonuclease PLD3 (PLD3)					26.9
LDTP03198	Ferrochelatase, mitochondrial (FECH)					19.0
LDTP01803	Adenosine deaminase (ADA)					17.0
LDTP10329	Ceramide synthase 2 (CERS2)					15.6
LDTP09620	Soluble calcium-activated nucleotidase 1 (CANT1)					15.6
LDTP02713	Macrophage migration inhibitory factor (MIF)					12.0
LDTP00186	Alkyldihydroxyacetonephosphate synthase, peroxisomal (AGPS)					10.1
LDTP01687	Apoptosis-inducing factor 1, mitochondrial (AIFM1)					9.8
LDTP06648	Lanosterol 14-alpha demethylase (CYP51A1)					9.6
LDTP01241	Bis(monoacylglycero)phosphate synthase CLN5 (CLN5)					9.3
LDTP01039	Protein-S-isoprenylcysteine O-methyltransferase (ICMT)					9.2
LDTP09043	Lysine-specific histone demethylase 2 (KDM1B)					9.1
LDTP03540	Heme oxygenase 2 (HMOX2)					9.0
LDTP09256	Putative phospholipase B-like 2 (PLBD2)					8.8
LDTP16032	Retinoid-inducible serine carboxypeptidase (SCPEP1)					8.7
LDTP01982	NADH-ubiquinone oxidoreductase chain 1 (MT-ND1)					8.1
LDTP09088	Phospholipase A2 group XV (PLA2G15)					7.8
LDTP00886	Nardilysin (NRDC)					7.7
LDTP06227	Prostaglandin reductase 1 (PTGR1)					7.6
LDTP04388	Palmitoyl-protein thioesterase 1 (PPT1)					7.5
LDTP03394	Ceramide synthase 1 (CERS1)					7.4
LDTP04712	Puromycin-sensitive aminopeptidase (NPEPPS)					7.2
LDTP06150	DNA replication licensing factor MCM6 (MCM6)					7.1
LDTP02006	Tissue alpha-L-fucosidase (FUCA1)					7.1
LDTP15988	Probable serine carboxypeptidase CPVL (CPVL)					7.0
LDTP14276	NADH dehydrogenase 1 beta subcomplex subunit 9 (NDUFB9)					6.9
LDTP06618	ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP)					6.8
LDTP00988	Protein O-GlcNAcase (OGA)					6.8
LDTP04008	Endothelin-converting enzyme 1 (ECE1)					6.8
LDTP08979	Prostaglandin reductase 2 (PTGR2)					6.8
LDTP13751	Exonuclease 1 (EXO1)					6.7
LDTP00428	Tripeptidyl-peptidase 1 (TPP1)					6.7
LDTP12588	Isoleucine--tRNA ligase, mitochondrial (IARS2)					6.6
LDTP06720	Peroxisomal leader peptide-processing protease (TYSND1)					6.6
LDTP01300	Cartilage-associated protein (CRTAP)					6.5
LDTP05588	RNA cytosine C(5)-methyltransferase NSUN2 (NSUN2)					6.5
LDTP02175	Epoxide hydrolase 1 (EPHX1)					6.4
LDTP02359	Heme oxygenase 1 (HMOX1)					6.4
LDTP12363	Lysophosphatidic acid phosphatase type 6 (ACP6)					6.4
LDTP10495	Ubiquitin carboxyl-terminal hydrolase 47 (USP47)					6.1
LDTP02169	Beta-hexosaminidase subunit alpha (HEXA)					6.0
LDTP13620	Lysosomal thioesterase PPT2 (PPT2)					6.0
LDTP04211	Isocitrate dehydrogenase [NADP], mitochondrial (IDH2)					5.9
LDTP00575	Glutathione S-transferase A4 (GSTA4)					5.8
LDTP02679	Prolyl 4-hydroxylase subunit alpha-1 (P4HA1)					5.8
LDTP02505	Lysosomal protective protein (CTSA)					5.8
LDTP15422	Prostaglandin reductase 3 (PTGR3)					5.8
LDTP03257	Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2)					5.7
LDTP13358	Leucyl-cystinyl aminopeptidase (LNPEP)					5.7
LDTP10862	Proteasome subunit beta type-7 (PSMB7)					5.6
LDTP07003	Presequence protease, mitochondrial (PITRM1)					5.6
LDTP13154	E3 ubiquitin-protein ligase RNF14 (RNF14)					5.5
LDTP13968	Exosome complex component CSL4 (EXOSC1)					5.4

Target ID	Target Name					Binding Ratio
LDTP07531	Sideroflexin-4 (SFXN4)					14.3
LDTP17362	Magnesium transporter NIPA3 (NIPAL1)					13.6
LDTP10913	Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A)					10.1
LDTP00865	Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20)					9.8
LDTP12383	Protein GPR108 (GPR108)					9.7
LDTP14952	Mpv17-like protein 2 (MPV17L2)					9.6
LDTP03132	Voltage-dependent anion-selective channel protein 1 (VDAC1)					9.1
LDTP01059	Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B)					8.8
LDTP06034	Lysosome membrane protein 2 (SCARB2)					8.5
LDTP17641	Solute carrier family 35 member F2 (SLC35F2)					8.5
LDTP09547	Scavenger receptor class B member 1 (SCARB1)					8.3
LDTP08016	Proton-coupled amino acid transporter 1 (SLC36A1)					8.2
LDTP04071	Voltage-dependent anion-selective channel protein 2 (VDAC2)					7.6
LDTP07236	Protein GPR107 (GPR107)					7.4
LDTP08881	Transmembrane protein 199 (TMEM199)					6.9
LDTP00541	NPC intracellular cholesterol transporter 1 (NPC1)					6.4
LDTP06308	Mitochondrial inner membrane protein OXA1L (OXA1L)					5.8
LDTP15481	Transmembrane protein 87A (TMEM87A)					5.8
LDTP03559	High affinity cationic amino acid transporter 1 (SLC7A1)					5.7
LDTP11813	Solute carrier family 25 member 32 (SLC25A32)					5.6
LDTP18448	Transmembrane 9 superfamily member 3 (TM9SF3)					5.4

Target ID	Target Name					Binding Ratio
LDTP15623	Neuferricin (CYB5D2)					18.1
LDTP14486	Clustered mitochondria protein homolog (CLUH)					13.4
LDTP00105	Protein unc-119 homolog B (UNC119B)					9.6
LDTP04094	Large proline-rich protein BAG6 (BAG6)					8.8
LDTP08081	Hepatoma-derived growth factor-related protein 2 (HDGFL2)					8.2
LDTP14792	Small ribosomal subunit protein uS9m (MRPS9)					7.5
LDTP16099	p53 and DNA damage-regulated protein 1 (PDRG1)					7.4
LDTP12468	Diablo IAP-binding mitochondrial protein (DIABLO)					7.0
LDTP11076	Apolipoprotein L2 (APOL2)					6.8
LDTP11239	Protein misato homolog 1 (MSTO1)					6.1
LDTP09881	TATA-binding protein-associated factor 2N (TAF15)					5.8
LDTP01652	Centrosomal protein 43 (CEP43)					5.7
LDTP02913	Desmin (DES)					5.6
LDTP11978	HAUS augmin-like complex subunit 4 (HAUS4)					5.4
LDTP05160	Nucleobindin-2 (NUCB2)					5.4
LDTP16006	Optic atrophy 3 protein (OPA3)					5.4

1	Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26. Mass spectrometry data entry: PXD041587