Details of the Target

General Information of Target

Target ID LDTP09620
Target Name Soluble calcium-activated nucleotidase 1 (CANT1)
Gene Name CANT1
Gene ID 124583
Synonyms
SHAPY; Soluble calcium-activated nucleotidase 1; SCAN-1; EC 3.6.1.6; Apyrase homolog; Putative MAPK-activating protein PM09; Putative NF-kappa-B-activating protein 107
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPVQLSEHPEWNESMHSLRISVGGLPVLASMTKAADPRFRPRWKVILTFFVGAAILWLLC
SHRPAPGRPPTHNAHNWRLGQAPANWYNDTYPLSPPQRTPAGIRYRIAVIADLDTESRAQ
EENTWFSYLKKGYLTLSDSGDKVAVEWDKDHGVLESHLAEKGRGMELSDLIVFNGKLYSV
DDRTGVVYQIEGSKAVPWVILSDGDGTVEKGFKAEWLAVKDERLYVGGLGKEWTTTTGDV
VNENPEWVKVVGYKGSVDHENWVSNYNALRAAAGIQPPGYLIHESACWSDTLQRWFFLPR
RASQERYSEKDDERKGANLLLSASPDFGDIAVSHVGAVVPTHGFSSFKFIPNTDDQIIVA
LKSEEDSGRVASYIMAFTLDGRFLLPETKIGSVKYEGIEFI
Target Bioclass
Enzyme
Family
Apyrase family
Subcellular location
Endoplasmic reticulum membrane
Function
Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP. Involved in proteoglycan synthesis.
Uniprot ID
Q8WVQ1
Ensemble ID
ENST00000302345.6
HGNC ID
HGNC:19721

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.D312G .
JVM3 SNV: p.E215Ter .
KMRC1 SNV: p.S345L .
NCIH716 SNV: p.I103V .
OVK18 Deletion: p.L93CfsTer37 .
SKMEL24 Substitution: p.G23K .
SUPT1 Deletion: p.K131RfsTer4 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info  		2.27  LDD0318  [1]
FBP2
 Probe Info  		3.95  LDD0317  [1]
Curcusone 37
 Probe Info  		4.23  LDD0188  [2]
Alkyne-RA190
 Probe Info  		2.24  LDD0302  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [4]
AOyne
 Probe Info  		12.20  LDD0443  [5]
PAL-AfBPP Probe
Click To Hide/Show 16 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C010
 Probe Info  		5.10  LDD1719  [6]
C022
 Probe Info  		7.73  LDD1728  [6]
C040
 Probe Info  		20.39  LDD1740  [6]
C041
 Probe Info  		6.54  LDD1741  [6]
C112
 Probe Info  		17.51  LDD1799  [6]
C169
 Probe Info  		23.10  LDD1849  [6]
C170
 Probe Info  		9.19  LDD1850  [6]
C210
 Probe Info  		64.89  LDD1884  [6]
C239
 Probe Info  		15.56  LDD1912  [6]
C278
 Probe Info  		53.45  LDD1948  [6]
C310
 Probe Info  		11.55  LDD1977  [6]
C355
 Probe Info  		30.06  LDD2016  [6]
C356
 Probe Info  		8.57  LDD2017  [6]
C362
 Probe Info  		34.06  LDD2023  [6]
C388
 Probe Info  		45.57  LDD2047  [6]
C403
 Probe Info  		18.51  LDD2061  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0033  Curcusone1d MCF-7 4.23  LDD0188  [2]
 LDCM0131  RA190 SK-MEL-5 2.24  LDD0302  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Clarin-1 (CLRN1) Clarin family P58418
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein FAM209A (FAM209A) FAM209 family Q5JX71

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Phosphomethylphosphonic Acid Guanosyl Ester . DB03486

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 Total Synthesis and Target Identification of the Curcusone Diterpenes. J Am Chem Soc. 2021 Mar 24;143(11):4379-4386. doi: 10.1021/jacs.1c00557. Epub 2021 Mar 11.
3 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
6 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587