Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C010  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 45 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP12020  Histone-lysine N-methyltransferase SMYD3 (SMYD3) 15.9
 LDTP02837  Beta-galactosidase (GLB1) 14.8
 LDTP08728  Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) 14.3
 LDTP09088  Phospholipase A2 group XV (PLA2G15) 13.7
 LDTP15988  Probable serine carboxypeptidase CPVL (CPVL) 12.6
 LDTP06333  Serum paraoxonase/arylesterase 2 (PON2) 11.5
 LDTP03540  Heme oxygenase 2 (HMOX2) 11.2
 LDTP04004  Lysosomal Pro-X carboxypeptidase (PRCP) 10.6
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 10.1
 LDTP02359  Heme oxygenase 1 (HMOX1) 9.4
 LDTP12013  dCTP pyrophosphatase 1 (DCTPP1) 8.1
 LDTP04008  Endothelin-converting enzyme 1 (ECE1) 8.0
 LDTP04285  Proteasome subunit beta type-2 (PSMB2) 8.0
 LDTP04388  Palmitoyl-protein thioesterase 1 (PPT1) 7.9
 LDTP00697  Cytochrome b5 type B (CYB5B) 7.8
 LDTP02505  Lysosomal protective protein (CTSA) 7.8
 LDTP05758  S-methyl-5'-thioadenosine phosphorylase (MTAP) 7.8
 LDTP14218  Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) 7.7
 LDTP10329  Ceramide synthase 2 (CERS2) 7.7
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 7.4
 LDTP09256  Putative phospholipase B-like 2 (PLBD2) 7.2
 LDTP04737  DNA polymerase epsilon subunit 2 (POLE2) 7.2
 LDTP02005  Lysosomal acid glucosylceramidase (GBA1) 6.7
 LDTP01241  Bis(monoacylglycero)phosphate synthase CLN5 (CLN5) 6.6
 LDTP16032  Retinoid-inducible serine carboxypeptidase (SCPEP1) 6.6
 LDTP01769  Dihydrofolate reductase (DHFR) 6.4
 LDTP00988  Protein O-GlcNAcase (OGA) 6.4
 LDTP12040  Prostaglandin E synthase 2 (PTGES2) 6.3
 LDTP16007  Lipid droplet-associated hydrolase (LDAH) 6.2
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 6.2
 LDTP02539  Lysosomal acid phosphatase (ACP2) 6.1
 LDTP05128  Glutathione S-transferase omega-1 (GSTO1) 5.9
 LDTP13751  Exonuclease 1 (EXO1) 5.8
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 5.7
 LDTP01707  Thioredoxin domain-containing protein 12 (TXNDC12) 5.5
 LDTP00783  Beta-1,4-glucuronyltransferase 1 (B4GAT1) 5.4
 LDTP12363  Lysophosphatidic acid phosphatase type 6 (ACP6) 5.4
 LDTP06618  ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) 5.4
 LDTP00910  Peroxisomal ATPase PEX1 (PEX1) 5.3
 LDTP04712  Puromycin-sensitive aminopeptidase (NPEPPS) 5.3
 LDTP05029  Peptidyl-prolyl cis-trans isomerase FKBP1A (FKBP1A) 5.2
 LDTP05066  Signal peptidase complex catalytic subunit SEC11A (SEC11A) 5.2
 LDTP05905  Acid ceramidase (ASAH1) 5.2
 LDTP11273  N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) 5.1
 LDTP09620  Soluble calcium-activated nucleotidase 1 (CANT1) 5.1
Transporter and channel
Click to Show/Hide to Show 32 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 16.8
 LDTP06034  Lysosome membrane protein 2 (SCARB2) 14.4
 LDTP06945  Sigma intracellular receptor 2 (TMEM97) 14.0
 LDTP09547  Scavenger receptor class B member 1 (SCARB1) 13.9
 LDTP06426  Translocating chain-associated membrane protein 1 (TRAM1) 12.6
 LDTP14511  ATP synthase subunit g, mitochondrial (ATP5MG) 10.8
 LDTP10958  Sigma non-opioid intracellular receptor 1 (SIGMAR1) 9.6
 LDTP07236  Protein GPR107 (GPR107) 9.2
 LDTP16813  Tetraspanin-3 (TSPAN3) 9.1
 LDTP02724  Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) 8.6
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 8.6
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 8.5
 LDTP06221  StAR-related lipid transfer protein 3 (STARD3) 8.3
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 8.0
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 8.0
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 7.4
 LDTP12383  Protein GPR108 (GPR108) 7.0
 LDTP00592  Surfeit locus protein 4 (SURF4) 6.4
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 6.3
 LDTP17641  Solute carrier family 35 member F2 (SLC35F2) 6.1
 LDTP04463  H(+)/Cl(-) exchange transporter 7 (CLCN7) 6.0
 LDTP02645  Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1) 5.9
 LDTP15648  BRI3-binding protein (BRI3BP) 5.8
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 5.7
 LDTP11615  Protein tweety homolog 3 (TTYH3) 5.6
 LDTP11213  Nucleoporin NDC1 (NDC1) 5.5
 LDTP03559  High affinity cationic amino acid transporter 1 (SLC7A1) 5.5
 LDTP11813  Solute carrier family 25 member 32 (SLC25A32) 5.4
 LDTP08881  Transmembrane protein 199 (TMEM199) 5.4
 LDTP13174  Mitochondrial dicarboxylate carrier (SLC25A10) 5.3
 LDTP05900  Metaxin-1 (MTX1) 5.2
 LDTP00541  NPC intracellular cholesterol transporter 1 (NPC1) 5.2
Transcription factor
Click to Show/Hide to Show 1 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP16717  Forkhead-associated domain-containing protein 1 (FHAD1) 19.2
Other
Click to Show/Hide to Show 29 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 18.8
 LDTP05160  Nucleobindin-2 (NUCB2) 18.0
 LDTP03678  ER lumen protein-retaining receptor 2 (KDELR2) 16.1
 LDTP11076  Apolipoprotein L2 (APOL2) 15.9
 LDTP13606  Mammalian ependymin-related protein 1 (EPDR1) 12.6
 LDTP13755  Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) 12.1
 LDTP01652  Centrosomal protein 43 (CEP43) 9.3
 LDTP13902  Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) 9.1
 LDTP19780  Keratinocyte-associated transmembrane protein 2 (KCT2) 9.1
 LDTP04094  Large proline-rich protein BAG6 (BAG6) 8.3
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 8.2
 LDTP01225  Mediator of RNA polymerase II transcription subunit 24 (MED24) 7.5
 LDTP13630  Neudesin (NENF) 7.5
 LDTP01522  Reticulon-3 (RTN3) 7.5
 LDTP00281  Golgi integral membrane protein 4 (GOLIM4) 7.0
 LDTP05375  Nucleobindin-1 (NUCB1) 6.9
 LDTP14458  Tetraspanin-6 (TSPAN6) 6.9
 LDTP19021  EF-hand calcium-binding domain-containing protein 14 (EFCAB14) 6.8
 LDTP14058  Rap guanine nucleotide exchange factor 2 (RAPGEF2) 6.6
 LDTP16080  Protein FAM114A2 (FAM114A2) 6.4
 LDTP16192  Mitochondrial fission process protein 1 (MTFP1) 6.3
 LDTP07368  Tetratricopeptide repeat protein 19, mitochondrial (TTC19) 5.9
 LDTP07906  Mediator of RNA polymerase II transcription subunit 25 (MED25) 5.7
 LDTP13086  Syntaxin-18 (STX18) 5.6
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 5.5
 LDTP07839  Capping protein-inhibiting regulator of actin dynamics (CRACD) 5.3
 LDTP17580  FHF complex subunit HOOK-interacting protein 2B (FHIP2B) 5.3
 LDTP13807  PRELI domain-containing protein 1, mitochondrial (PRELID1) 5.2
 LDTP13402  Dynactin subunit 4 (DCTN4) 5.1

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587