Details of the Target

General Information of Target

Target ID LDTP07906
Target Name Mediator of RNA polymerase II transcription subunit 25 (MED25)
Gene Name MED25
Gene ID 81857
Synonyms
ACID1; ARC92; PTOV2; Mediator of RNA polymerase II transcription subunit 25; Activator interaction domain-containing protein 1; Activator-recruited cofactor 92 kDa component; ARC92; Mediator complex subunit 25; p78
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVPGSEGPARAGSVVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGD
YGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLST
ALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHF
SIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPRHMVLVRGLVLPVGGGSAPGPLQ
SKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLG
PRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTA
QPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDAN
TKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDL
ESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQV
ITNHKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASG
ATGQPQPQGTAQPPPGAPQGPPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGV
PPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPGQMLL
SGGPRGPVPQPGLQPSVMEDDILMDLI
Target Bioclass
Other
Family
Mediator complex subunit 25 family
Subcellular location
Nucleus
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.
Uniprot ID
Q71SY5
Ensemble ID
ENST00000312865.10
HGNC ID
HGNC:28845
ChEMBL ID
CHEMBL5169169

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
MELJUSO SNV: p.P629L DBIA    Probe Info 
MOLT4 Deletion: p.T64PfsTer150; p.K187SfsTer27 IA-alkyne    Probe Info 
NOMO1 SNV: p.G237A .
RKO Deletion: p.P566QfsTer31 DBIA    Probe Info 
RPMI8226 SNV: p.A504V DBIA    Probe Info 
SKMEL28 SNV: p.L278V DBIA    Probe Info 
TE11 SNV: p.G722R DBIA    Probe Info 
TOV21G SNV: p.S258Ter DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		13.89  LDD0402  [1]
STPyne
 Probe Info  		K545(5.00)  LDD0277  [2]
DBIA
 Probe Info  		C429(2.04)  LDD3312  [3]
BTD
 Probe Info  		C429(1.22)  LDD2094  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
IPM
 Probe Info  		N.A.  LDD0147  [6]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [7]
NAIA_5
 Probe Info  		N.A.  LDD2223  [8]
PAL-AfBPP Probe
Click To Hide/Show 21 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C008
 Probe Info  		5.35  LDD1717  [9]
C010
 Probe Info  		5.70  LDD1719  [9]
C059
 Probe Info  		36.00  LDD1756  [9]
C064
 Probe Info  		5.98  LDD1761  [9]
C067
 Probe Info  		5.54  LDD1763  [9]
C070
 Probe Info  		13.45  LDD1766  [9]
C082
 Probe Info  		6.41  LDD1774  [9]
C083
 Probe Info  		5.24  LDD1775  [9]
C085
 Probe Info  		9.58  LDD1777  [9]
C107
 Probe Info  		10.93  LDD1794  [9]
C108
 Probe Info  		8.28  LDD1795  [9]
C130
 Probe Info  		6.82  LDD1812  [9]
C160
 Probe Info  		6.87  LDD1840  [9]
C178
 Probe Info  		16.45  LDD1857  [9]
C198
 Probe Info  		14.12  LDD1874  [9]
C219
 Probe Info  		10.34  LDD1893  [9]
C220
 Probe Info  		12.21  LDD1894  [9]
C277
 Probe Info  		19.29  LDD1947  [9]
C363
 Probe Info  		41.64  LDD2024  [9]
AEA-DA
 Probe Info  		5.18  LDD0333  [10]
OEA-DA
 Probe Info  		20.00  LDD0046  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C429(1.08)  LDD2103  [4]
 LDCM0572  Fragment10 Ramos C429(1.75)  LDD2189  [11]
 LDCM0573  Fragment11 Ramos C429(2.99)  LDD2190  [11]
 LDCM0575  Fragment13 Ramos C429(0.88)  LDD2192  [11]
 LDCM0576  Fragment14 Ramos C429(1.07)  LDD2193  [11]
 LDCM0580  Fragment21 Ramos C429(1.07)  LDD2195  [11]
 LDCM0582  Fragment23 Ramos C429(0.76)  LDD2196  [11]
 LDCM0578  Fragment27 Ramos C429(0.85)  LDD2197  [11]
 LDCM0586  Fragment28 Ramos C429(0.65)  LDD2198  [11]
 LDCM0588  Fragment30 Ramos C429(1.20)  LDD2199  [11]
 LDCM0589  Fragment31 Ramos C429(0.93)  LDD2200  [11]
 LDCM0468  Fragment33 Ramos C429(1.13)  LDD2202  [11]
 LDCM0596  Fragment38 Ramos C429(0.91)  LDD2203  [11]
 LDCM0610  Fragment52 Ramos C429(2.52)  LDD2204  [11]
 LDCM0614  Fragment56 Ramos C429(1.13)  LDD2205  [11]
 LDCM0571  Fragment9 Ramos C429(1.91)  LDD2188  [11]
 LDCM0022  KB02 HEK-293T C429(0.98)  LDD1492  [12]
 LDCM0023  KB03 HEK-293T C429(1.00)  LDD1497  [12]
 LDCM0024  KB05 HMCB C429(2.04)  LDD3312  [3]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C429(1.22)  LDD2094  [4]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C429(0.72)  LDD2098  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C429(0.74)  LDD2109  [4]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C429(0.74)  LDD2128  [4]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C429(1.00)  LDD2129  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C429(0.91)  LDD2146  [4]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C429(2.08)  LDD2153  [4]
 LDCM0136  SR-4559 HEK-293T 5.18  LDD0333  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) . O00308
Transcription factor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Retinoic acid receptor alpha (RARA) Nuclear hormone receptor family P10276
Retinoic acid receptor RXR-alpha (RXRA) Nuclear hormone receptor family P19793
Signal transducer and activator of transcription 5B (STAT5B) Transcription factor STAT family P51692
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Oxoeicosanoid receptor 1 (OXER1) G-protein coupled receptor 1 family Q8TDS5
Other
Click To Hide/Show 16 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Epithelial splicing regulatory protein 1 (ESRP1) ESRP family Q6NXG1
Keratin-associated protein 6-1 (KRTAP6-1) KRTAP type 6 family Q3LI64
Mediator of RNA polymerase II transcription subunit 14 (MED14) Mediator complex subunit 14 family O60244
Mediator of RNA polymerase II transcription subunit 15 (MED15) Mediator complex subunit 15 family Q96RN5
Mediator of RNA polymerase II transcription subunit 16 (MED16) Mediator complex subunit 16 family Q9Y2X0
Mediator of RNA polymerase II transcription subunit 23 (MED23) Mediator complex subunit 23 family Q9ULK4
Mediator of RNA polymerase II transcription subunit 24 (MED24) Mediator complex subunit 24 family O75448
Odontogenic ameloblast-associated protein (ODAM) ODAM family A1E959
AP-4 complex accessory subunit RUSC1 (RUSC1) . Q9BVN2
Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) . O14964
Kelch-like protein 12 (KLHL12) . Q53G59
Peflin (PEF1) . Q9UBV8
Pleckstrin homology domain-containing family B member 2 (PLEKHB2) . Q96CS7
Protein TFG (TFG) . Q92734
SERTA domain-containing protein 3 (SERTAD3) . Q9UJW9

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
10 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
11 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402