Details of the Target

General Information of Target

Target ID LDTP10116
Target Name Lipid droplet assembly factor 1 (LDAF1)
Gene Name LDAF1
Gene ID 57146
Synonyms
TMEM159; Lipid droplet assembly factor 1; Promethin; Transmembrane protein 159
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQPPRERLVVTGRAGWMGMGRGAGRSALGFWPTLAFLLCSFPAATSPCKILKCNSEFWSA
TSGSHAPASDDTPEFCAALRSYALCTRRTARTCRGDLAYHSAVHGIEDLMSQHNCSKDGP
TSQPRLRTLPPAGDSQERSDSPEICHYEKSFHKHSATPNYTHCGLFGDPHLRTFTDRFQT
CKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQECVDQKVYQAEMDELP
AAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEV
VNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETA
VAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYERTRDL
PGRAAAGLPLAPRPLLGALVPLLALLPVFC
Target Bioclass
Other
Family
LDAF1 family
Subcellular location
Endoplasmic reticulum membrane
Function
Plays an important role in the formation of lipid droplets (LD) which are storage organelles at the center of lipid and energy homeostasis. In association with BSCL2/seipin, defines the sites of LD formation in the endoplasmic reticulum.
Uniprot ID
Q96B96
Ensemble ID
ENST00000233047.9
HGNC ID
HGNC:30136

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
ETK1 SNV: p.S93T .
MEC1 SNV: p.L78P .
SSP25 SNV: p.S93T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K19(2.38)  LDD0277  [1]
NAIA_5
 Probe Info  		C144(1.19)  LDD2228  [2]
PAL-AfBPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C055
 Probe Info  		14.12  LDD1752  [3]
C056
 Probe Info  		20.53  LDD1753  [3]
C112
 Probe Info  		24.42  LDD1799  [3]
C169
 Probe Info  		28.05  LDD1849  [3]
C225
 Probe Info  		5.90  LDD1898  [3]
C226
 Probe Info  		6.32  LDD1899  [3]
C228
 Probe Info  		28.64  LDD1901  [3]
C264
 Probe Info  		28.25  LDD1935  [3]
C285
 Probe Info  		33.36  LDD1955  [3]
C287
 Probe Info  		22.94  LDD1957  [3]
C288
 Probe Info  		8.51  LDD1958  [3]
C289
 Probe Info  		69.55  LDD1959  [3]
C290
 Probe Info  		4.99  LDD1960  [3]
C299
 Probe Info  		8.94  LDD1968  [3]
C373
 Probe Info  		8.00  LDD2033  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0634  CY-0357 Hep-G2 C144(1.19)  LDD2228  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glutamate decarboxylase 2 (GAD2) Group II decarboxylase family Q05329
Atypical kinase COQ8A, mitochondrial (COQ8A) ADCK protein kinase family Q8NI60
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Calcium channel flower homolog (CACFD1) Calcium channel flower family Q9UGQ2
Heme transporter HRG1 (SLC48A1) HRG family Q6P1K1
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Vesicle-trafficking protein SEC22a (SEC22A) Synaptobrevin family Q96IW7
Synaptotagmin-16 (SYT16) Synaptotagmin family Q17RD7
Transmembrane protein 19 (TMEM19) TMEM19 family Q96HH6

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587