Details of the Target

General Information of Target

Target ID LDTP13309
Target Name Prefoldin subunit 2 (PFDN2)
Gene Name PFDN2
Gene ID 5202
Synonyms
PFD2; Prefoldin subunit 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSSLPVPYKLPVSLSVGSCVIIKGTPIHSFINDPQLQVDFYTDMDEDSDIAFRFRVHFGN
HVVMNRREFGIWMLEETTDYVPFEDGKQFELCIYVHYNEYEIKVNGIRIYGFVHRIPPSF
VKMVQVSRDISLTSVCVCN
Target Bioclass
Other
Family
Prefoldin subunit beta family
Subcellular location
Nucleus
Function
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Uniprot ID
Q9UHV9
Ensemble ID
ENST00000368010.4
HGNC ID
HGNC:8867
ChEMBL ID
CHEMBL4295976

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
FBPP2
 Probe Info  		2.25  LDD0318  [2]
SAA-alkyne
 Probe Info  		1.10  LDD0252  [3]
AZ-9
 Probe Info  		E80(10.00); E102(10.00)  LDD2209  [4]
BTD
 Probe Info  		C70(0.08)  LDD2096  [5]
DA-P3
 Probe Info  		4.71  LDD0183  [6]
EA-probe
 Probe Info  		N.A.  LDD0440  [7]
ATP probe
 Probe Info  		K18(0.00); K94(0.00); K136(0.00); K10(0.00)  LDD0199  [8]
1d-yne
 Probe Info  		N.A.  LDD0358  [9]
ATP probe
 Probe Info  		N.A.  LDD0035  [10]
NHS
 Probe Info  		N.A.  LDD0010  [11]
SF
 Probe Info  		N.A.  LDD0028  [12]
STPyne
 Probe Info  		N.A.  LDD0009  [11]
1c-yne
 Probe Info  		K84(0.00); K43(0.00); K94(0.00); K18(0.00)  LDD0228  [9]
HHS-465
 Probe Info  		N.A.  LDD2240  [13]
PAL-AfBPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C087
 Probe Info  		12.64  LDD1779  [14]
C249
 Probe Info  		14.42  LDD1922  [14]
C269
 Probe Info  		5.66  LDD1939  [14]
C284
 Probe Info  		26.17  LDD1954  [14]
C305
 Probe Info  		10.48  LDD1974  [14]
C313
 Probe Info  		14.03  LDD1980  [14]
C354
 Probe Info  		7.01  LDD2015  [14]
C391
 Probe Info  		12.47  LDD2050  [14]
C403
 Probe Info  		14.03  LDD2061  [14]
C407
 Probe Info  		11.08  LDD2064  [14]
FFF probe11
 Probe Info  		8.42  LDD0472  [15]
FFF probe13
 Probe Info  		20.00  LDD0475  [15]
FFF probe14
 Probe Info  		20.00  LDD0477  [15]
FFF probe2
 Probe Info  		19.19  LDD0463  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 13.88  LDD0403  [1]
 LDCM0031  Epigallocatechin gallate HEK-293T 4.71  LDD0183  [6]
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [7]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C70(0.08)  LDD2096  [5]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C70(0.09)  LDD2106  [5]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C70(0.19)  LDD2116  [5]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C70(0.15)  LDD2118  [5]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C70(1.00)  LDD2120  [5]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C70(0.14)  LDD2122  [5]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C70(0.10)  LDD2124  [5]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C70(0.11)  LDD2126  [5]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C70(0.33)  LDD2128  [5]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C70(0.14)  LDD2149  [5]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C70(0.12)  LDD2151  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytochrome b-c1 complex subunit 1, mitochondrial (UQCRC1) Peptidase M16 family P31930
Unconventional prefoldin RPB5 interactor 1 (URI1) RNA polymerase II subunit 5-mediating protein family O94763
Ras-related C3 botulinum toxin substrate 1 (RAC1) Rho family P63000
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Prefoldin subunit 3 (VBP1) Prefoldin subunit alpha family P61758
Prefoldin subunit 5 (PFDN5) Prefoldin subunit alpha family Q99471
p53 and DNA damage-regulated protein 1 (PDRG1) Prefoldin subunit beta family Q9NUG6
Prefoldin subunit 1 (PFDN1) Prefoldin subunit beta family O60925
RNA polymerase II-associated protein 3 (RPAP3) RPAP3 family Q9H6T3
Protein UXT (UXT) UXT family Q9UBK9

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
3 Chemoproteomics and Phosphoproteomics Profiling Reveals Salvianolic Acid A as a Covalent Inhibitor of mTORC1. J Proteome Res. 2023 Jul 7;22(7):2450-2459. doi: 10.1021/acs.jproteome.3c00188. Epub 2023 Jun 22.
4 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
7 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
8 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
9 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
10 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
11 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
12 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
13 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
14 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
15 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.