Details of the Target

General Information of Target

Target ID LDTP00913
Target Name Mitochondrial import inner membrane translocase subunit Tim8 A (TIMM8A)
Gene Name TIMM8A
Gene ID 1678
Synonyms
DDP; DDP1; TIM8A; Mitochondrial import inner membrane translocase subunit Tim8 A; Deafness dystonia protein 1; X-linked deafness dystonia protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDSSSSSSAAGLGAVDPQLQHFIEVETQKQRFQQLVHQMTELCWEKCMDKPGPKLDSRAE
ACFVNCVERFIDTSQFILNRLEQTQKSKPVFSESLSD
Target Bioclass
Other
Family
Small Tim family
Subcellular location
Mitochondrion inner membrane
Function
Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins. Probably necessary for normal neurologic development.
Uniprot ID
O60220
Ensemble ID
ENST00000372902.4
HGNC ID
HGNC:11817

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
ONAyne
 Probe Info  		K86(0.51)  LDD0274  [1]
Acrolein
 Probe Info  		C43(0.00); C66(0.00)  LDD0222  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [3]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [4]
TFBX
 Probe Info  		C62(0.00); C66(0.00)  LDD0027  [4]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [5]
Methacrolein
 Probe Info  		N.A.  LDD0218  [2]
NAIA_5
 Probe Info  		C66(0.00); C62(0.00); C47(0.00); C43(0.00)  LDD2223  [6]
PAL-AfBPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C106
 Probe Info  		24.76  LDD1793  [7]
C108
 Probe Info  		8.46  LDD1795  [7]
C232
 Probe Info  		37.27  LDD1905  [7]
C293
 Probe Info  		20.11  LDD1963  [7]
C296
 Probe Info  		14.32  LDD1966  [7]
C313
 Probe Info  		15.14  LDD1980  [7]
C314
 Probe Info  		13.55  LDD1981  [7]
C343
 Probe Info  		12.04  LDD2005  [7]
C348
 Probe Info  		11.47  LDD2009  [7]
C349
 Probe Info  		10.70  LDD2010  [7]
C350
 Probe Info  		37.53  LDD2011  [7]
C354
 Probe Info  		7.26  LDD2015  [7]
C362
 Probe Info  		25.28  LDD2023  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa C43(0.00); C66(0.00)  LDD0222  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
mRNA export factor GLE1 (GLE1) GLE1 family Q53GS7
Huntingtin (HTT) Huntingtin family P42858
Wolframin (WFS1) . O76024
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
AT-rich interactive domain-containing protein 3A (ARID3A) . Q99856
Other
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein BRICK1 (BRK1) BRK1 family Q8WUW1
Caveolae-associated protein 3 (CAVIN3) CAVIN family Q969G5
Keratin, type I cytoskeletal 15 (KRT15) Intermediate filament family P19012
Protein Mis18-beta (OIP5) Mis18 family O43482
Mitochondrial import inner membrane translocase subunit Tim13 (TIMM13) Small Tim family Q9Y5L4
Mitochondrial import inner membrane translocase subunit Tim8 A (TIMM8A) Small Tim family O60220
Signal transducing adapter molecule 2 (STAM2) STAM family O75886
T-complex protein 1 subunit epsilon (CCT5) TCP-1 chaperonin family P48643
Complement C1q tumor necrosis factor-related protein 2 (C1QTNF2) . Q9BXJ5
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) . Q7Z699

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587