Details of the Target

General Information of Target

Target ID LDTP11764
Target Name Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (NUCKS1)
Gene Name NUCKS1
Gene ID 64710
Synonyms
NUCKS; Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1; P1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAPARAGFCPLLLLLLLGLWVAEIPVSAKPKGMTSSQWFKIQHMQPSPQACNSAMKNINK
HTKRCKDLNTFLHEPFSSVAATCQTPKIACKNGDKNCHQSHGAVSLTMCKLTSGKHPNCR
YKEKRQNKSYVVACKPPQKKDSQQFHLVPVHLDRVL
Target Bioclass
Other
Subcellular location
Nucleus
Function
Chromatin-associated protein involved in DNA repair by promoting homologous recombination (HR). Binds double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures, but with less affinity than RAD51AP1.
Uniprot ID
Q9H1E3
Ensemble ID
ENST00000367142.5
HGNC ID
HGNC:29923

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		11.81  LDD0066  [1]
ONAyne
 Probe Info  		K87(10.00)  LDD0274  [2]
STPyne
 Probe Info  		K171(9.19); K175(7.99); K184(2.90); K188(6.67)  LDD0277  [2]
AZ-9
 Probe Info  		E18(1.00)  LDD2208  [3]
Probe 1
 Probe Info  		Y13(13.05); Y26(57.38)  LDD3495  [4]
Acrolein
 Probe Info  		N.A.  LDD0221  [5]
AMP probe
 Probe Info  		K175(0.00); K184(0.00); K186(0.00); K9(0.00)  LDD0200  [6]
ATP probe
 Probe Info  		K175(0.00); K9(0.00); K184(0.00); K186(0.00)  LDD0199  [6]
ATP probe
 Probe Info  		N.A.  LDD0035  [7]
NHS
 Probe Info  		K184(0.00); K175(0.00); K9(0.00)  LDD0010  [8]
SF
 Probe Info  		Y26(0.00); K159(0.00); Y13(0.00)  LDD0028  [9]
1c-yne
 Probe Info  		N.A.  LDD0228  [10]
1d-yne
 Probe Info  		N.A.  LDD0357  [10]
HHS-465
 Probe Info  		K9(0.00); K98(0.00); Y13(0.00); K188(0.00)  LDD2240  [11]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C187
 Probe Info  		19.03  LDD1865  [12]
C191
 Probe Info  		11.16  LDD1868  [12]
C193
 Probe Info  		5.10  LDD1869  [12]
C354
 Probe Info  		8.57  LDD2015  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0107  IAA HeLa N.A.  LDD0221  [5]

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
7 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
10 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
11 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
12 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587