Details of the Target

General Information of Target

Target ID LDTP02181
Target Name Neurofilament medium polypeptide (NEFM)
Gene Name NEFM
Gene ID 4741
Synonyms
NEF3; NFM; Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSMLAPR
LAYSSAMLSSAESSLDFSQSSSLLNGGSGPGGDYKLSRSNEKEQLQGLNDRFAGYIEKVH
YLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEE
DIHRLKERFEEEARLRDDTEAAIRALRKDIEEASLVKVELDKKVQSLQDEVAFLRSNHEE
EVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESHSDQNMHQAEEWFKCRYAKLT
EAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQ
DTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSIT
GPLYTHRPPITISSKIQKPKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEEL
AVSMKEEKKEAAEEKEEEPEAEEEEVAAKKSPVKATAPEVKEEEGEKEEEEGQEEEEEED
EGAKSDQAEEGGSEKEGSSEKEEGEQEEGETEAEAEGEEAEAKEEKKVEEKSEEVATKEE
LVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSP
VEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEKEVKEAPKEEKVEK
KEEKPKDVPEKKKAESPVKEEAVAEVVTITKSVKVHLEKETKEEGKPLQQEKEKEKAGGE
GGSEEEGSDKGAKGSRKEDIAVNGEVEGKEEVEQETKEKGSGREEEKGVVTNGLDLSPAD
EKKGGDKSEEKVVVTKTVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKV
EKVTSHAIVKEVTQSD
Target Bioclass
Other
Family
Intermediate filament family
Subcellular location
Cytoplasm, cytoskeleton
Function
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Uniprot ID
P07197
Ensemble ID
ENST00000221166.10
HGNC ID
HGNC:7734

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.A213V; p.E319K .
AU565 SNV: p.K638Q .
CCK81 SNV: p.R16W; p.D197N .
COLO679 Deletion: p.F890del .
HCT15 SNV: p.A131V; p.N165S; p.A213V .
HEC1 SNV: p.E785Ter .
HEC1B SNV: p.A399T; p.E785Ter .
HG3 SNV: p.E498D .
HT29 SNV: p.P660Q .
KARPAS422 Substitution: p.S46P .
LS123 SNV: p.R382H .
LS180 SNV: p.G91D .
MDST8 SNV: p.E675K .
NCIH2172 SNV: p.K910N .
NCIH2286 SNV: p.M377K .
OVTOKO Deletion: p.E850del .
RKO SNV: p.G791C .
RL952 SNV: p.P59L .
SHP77 SNV: p.E723D .
SKN SNV: p.Q385K .
SKNSH SNV: p.F35L .
SNU1 SNV: p.G868C .
SUPT1 SNV: p.A71T .
WM793 SNV: p.S252L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
AZ-9
 Probe Info  		E383(10.00); D386(10.00)  LDD2208  [1]
ONAyne
 Probe Info  		K314(0.00); K102(0.00)  LDD0273  [2]
HHS-465
 Probe Info  		Y150(8.76); Y401(0.63)  LDD2237  [3]
1d-yne
 Probe Info  		N.A.  LDD0358  [4]
NHS
 Probe Info  		K871(0.00); K102(0.00); K298(0.00); K314(0.00)  LDD0010  [5]
SF
 Probe Info  		Y115(0.00); Y14(0.00); Y401(0.00); Y384(0.00)  LDD0028  [6]
STPyne
 Probe Info  		N.A.  LDD0009  [5]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [7]
1c-yne
 Probe Info  		K127(0.00); K223(0.00)  LDD0228  [4]
AOyne
 Probe Info  		14.90  LDD0443  [8]
PAL-AfBPP Probe
Click To Hide/Show 34 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C003
 Probe Info  		13.45  LDD1713  [9]
C052
 Probe Info  		6.28  LDD1750  [9]
C053
 Probe Info  		5.50  LDD1751  [9]
C055
 Probe Info  		12.55  LDD1752  [9]
C056
 Probe Info  		16.45  LDD1753  [9]
C087
 Probe Info  		20.25  LDD1779  [9]
C106
 Probe Info  		23.75  LDD1793  [9]
C108
 Probe Info  		10.27  LDD1795  [9]
C112
 Probe Info  		23.43  LDD1799  [9]
C158
 Probe Info  		9.78  LDD1838  [9]
C159
 Probe Info  		7.73  LDD1839  [9]
C160
 Probe Info  		7.73  LDD1840  [9]
C161
 Probe Info  		13.74  LDD1841  [9]
C187
 Probe Info  		20.39  LDD1865  [9]
C191
 Probe Info  		13.09  LDD1868  [9]
C193
 Probe Info  		4.99  LDD1869  [9]
C201
 Probe Info  		29.24  LDD1877  [9]
C206
 Probe Info  		17.27  LDD1881  [9]
C210
 Probe Info  		67.65  LDD1884  [9]
C214
 Probe Info  		5.62  LDD1888  [9]
C284
 Probe Info  		22.01  LDD1954  [9]
C313
 Probe Info  		23.43  LDD1980  [9]
C338
 Probe Info  		19.70  LDD2001  [9]
C355
 Probe Info  		25.81  LDD2016  [9]
C361
 Probe Info  		17.51  LDD2022  [9]
C362
 Probe Info  		35.02  LDD2023  [9]
C364
 Probe Info  		15.35  LDD2025  [9]
C391
 Probe Info  		18.38  LDD2050  [9]
C407
 Probe Info  		12.21  LDD2064  [9]
FFF probe11
 Probe Info  		16.59  LDD0471  [10]
FFF probe13
 Probe Info  		20.00  LDD0475  [10]
FFF probe2
 Probe Info  		20.00  LDD0463  [10]
FFF probe3
 Probe Info  		20.00  LDD0464  [10]
OEA-DA
 Probe Info  		16.52  LDD0046  [11]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transcription factor Jun (JUN) BZIP family P05412
Mothers against decapentaplegic homolog 2 (SMAD2) Dwarfin/SMAD family Q15796
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Neurofilament light polypeptide (NEFL) Intermediate filament family P07196
Vimentin (VIM) Intermediate filament family P08670
Retinoblastoma-associated protein (RB1) Retinoblastoma protein (RB) family P06400

References

1 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
4 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
7 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
10 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
11 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570