Probe Name	C256					Probe Info
Probe concentration	Probe:50uM; negative probe:50uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	TMT
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP09043	Lysine-specific histone demethylase 2 (KDM1B)					11.2
LDTP03787	Choline kinase alpha (CHKA)					10.5
LDTP10182	TLC domain-containing protein 1 (TLCD1)					8.3
LDTP09088	Phospholipase A2 group XV (PLA2G15)					6.8
LDTP10329	Ceramide synthase 2 (CERS2)					6.5
LDTP08979	Prostaglandin reductase 2 (PTGR2)					6.3
LDTP08476	5'-3' exonuclease PLD3 (PLD3)					5.3
LDTP02175	Epoxide hydrolase 1 (EPHX1)					5.2
LDTP06375	Delta(24)-sterol reductase (DHCR24)					5.2
LDTP04712	Puromycin-sensitive aminopeptidase (NPEPPS)					5.2
LDTP03417	Proteasome subunit beta type-4 (PSMB4)					5.1
LDTP04004	Lysosomal Pro-X carboxypeptidase (PRCP)					5.0

Target ID	Target Name					Binding Ratio
LDTP05900	Metaxin-1 (MTX1)					14.5
LDTP00865	Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20)					5.4
LDTP07885	Mitochondrial S-adenosylmethionine carrier protein (SLC25A26)					5.3
LDTP03061	Cation-dependent mannose-6-phosphate receptor (M6PR)					5.2
LDTP16069	Peroxisomal membrane protein 2 (PXMP2)					5.0

Target ID	Target Name					Binding Ratio
LDTP15623	Neuferricin (CYB5D2)					11.6
LDTP14486	Clustered mitochondria protein homolog (CLUH)					7.5
LDTP00105	Protein unc-119 homolog B (UNC119B)					7.2
LDTP05960	Trophoblast glycoprotein (TPBG)					5.5

1	Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26. Mass spectrometry data entry: PXD041587