Details of the Target

General Information of Target

Target ID LDTP01067
Target Name Endothelial differentiation-related factor 1 (EDF1)
Gene Name EDF1
Gene ID 8721
Synonyms
Endothelial differentiation-related factor 1; EDF-1; Multiprotein-bridging factor 1; MBF1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAESDWDTVTVLRKKGPTAAQAKSKQAILAAQRRGEDVETSKKWAAGQNKQHSITKNTAK
LDRETEELHHDRVTLEVGKVIQQGRQSKGLTQKDLATKINEKPQVIADYESGRAIPNNQV
LGKIERAIGLKLRGKDIGKPIEKGPRAK
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
Uniprot ID
O60869
Ensemble ID
ENST00000224073.6
HGNC ID
HGNC:3164
ChEMBL ID
CHEMBL4295670

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
Ishikawa (Heraklio) 02 ER SNV: p.R113W .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		9.91  LDD0066  [1]
ONAyne
 Probe Info  		K131(10.00)  LDD0274  [2]
STPyne
 Probe Info  		K102(1.69); K131(7.69); K135(5.40); K139(10.00)  LDD0277  [2]
AMP probe
 Probe Info  		N.A.  LDD0200  [3]
ATP probe
 Probe Info  		K15(0.00); K50(0.00); K56(0.00); K79(0.00)  LDD0199  [3]
m-APA
 Probe Info  		N.A.  LDD2232  [4]
ATP probe
 Probe Info  		N.A.  LDD0035  [5]
1d-yne
 Probe Info  		N.A.  LDD0356  [6]
SF
 Probe Info  		K25(0.00); K143(0.00)  LDD0028  [7]
Ox-W18
 Probe Info  		W6(0.00); W44(0.00)  LDD2175  [8]
1c-yne
 Probe Info  		K98(0.00); K102(0.00)  LDD0228  [6]
Acrolein
 Probe Info  		N.A.  LDD0217  [9]
PAL-AfBPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C003
 Probe Info  		33.36  LDD1713  [10]
C052
 Probe Info  		5.35  LDD1750  [10]
C087
 Probe Info  		8.75  LDD1779  [10]
C189
 Probe Info  		5.62  LDD1866  [10]
C362
 Probe Info  		27.47  LDD2023  [10]
C364
 Probe Info  		15.78  LDD2025  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H70(0.00); H69(0.00)  LDD0222  [9]
 LDCM0107  IAA HeLa H70(0.00); H69(0.00)  LDD0221  [9]
 LDCM0109  NEM HeLa H70(0.00); H69(0.00)  LDD0223  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Oxysterols receptor LXR-alpha (NR1H3) Nuclear hormone receptor family Q13133
Nuclear receptor subfamily 5 group A member 2 (NR5A2) Nuclear hormone receptor family O00482
Transcription factor
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Peroxisome proliferator-activated receptor gamma (PPARG) Nuclear hormone receptor family P37231
Estrogen receptor (ESR1) Nuclear hormone receptor family P03372
TATA-box-binding protein (TBP) TBP family P20226
Homeobox protein MOX-2 (MEOX2) . P50222

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
6 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
7 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
8 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
9 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587