Details of the Target

General Information of Target

Target ID LDTP08761
Target Name NADH dehydrogenase 1 alpha subcomplex assembly factor 2 (NDUFAF2)
Gene Name NDUFAF2
Gene ID 91942
Synonyms
NDUFA12L; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2; B17.2-like; B17.2L; Mimitin; Myc-induced mitochondrial protein; MMTN; NDUFA12-like protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGWSQDLFRALWRSLSREVKEHVGTDQFGNKYYYIPQYKNWRGQTIREKRIVEAANKKEV
DYEAGDIPTEWEAWIRRTRKTPPTMEEILKNEKHREEIKIKSQDFYEKEKLLSKETSEEL
LPPPVQTQIKGHASAPYFGKEEPSVAPSSTGKTFQPGSWMPRDGKSHNQ
Target Bioclass
Other
Family
Complex I NDUFA12 subunit family
Subcellular location
Mitochondrion
Function
Acts as a molecular chaperone for mitochondrial complex I assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
TTD ID
T01453
Uniprot ID
Q8N183
DrugMap ID
TTCHGVF
Ensemble ID
ENST00000296597.10
HGNC ID
HGNC:28086
ChEMBL ID
CHEMBL2363065

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y137(20.00); Y32(9.09); Y106(7.65); Y38(7.01)  LDD0260  [1]
STPyne
 Probe Info  		K101(2.70); K140(5.79); K20(3.52); K39(0.89)  LDD0277  [2]
5E-2FA
 Probe Info  		H22(0.00); H132(0.00)  LDD2235  [3]
ATP probe
 Probe Info  		N.A.  LDD0199  [4]
m-APA
 Probe Info  		H22(0.00); H132(0.00)  LDD2231  [3]
N1
 Probe Info  		N.A.  LDD0245  [5]
NHS
 Probe Info  		K140(0.00); K80(0.00)  LDD0010  [6]
SF
 Probe Info  		N.A.  LDD0028  [7]
1c-yne
 Probe Info  		N.A.  LDD0228  [8]
Acrolein
 Probe Info  		H22(0.00); H132(0.00)  LDD0217  [9]
PAL-AfBPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C003
 Probe Info  		12.91  LDD1713  [10]
C094
 Probe Info  		27.28  LDD1785  [10]
C112
 Probe Info  		18.38  LDD1799  [10]
C158
 Probe Info  		9.65  LDD1838  [10]
C210
 Probe Info  		51.27  LDD1884  [10]
C355
 Probe Info  		25.28  LDD2016  [10]
C361
 Probe Info  		18.00  LDD2022  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [9]
 LDCM0107  IAA HeLa N.A.  LDD0221  [9]
 LDCM0109  NEM HeLa H132(0.00); H22(0.00)  LDD0223  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Maspardin (SPG21) AB hydrolase superfamily Q9NZD8
Cytochrome b5 type B (CYB5B) Cytochrome b5 family O43169
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Small integral membrane protein 1 (SMIM1) SMIM1 family B2RUZ4
Sigma intracellular receptor 2 (TMEM97) TMEM97/sigma-2 receptor family Q5BJF2
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Lysophosphatidic acid receptor 3 (LPAR3) G-protein coupled receptor 1 family Q9UBY5
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Vesicle-trafficking protein SEC22b (SEC22B) Synaptobrevin family O75396
Syntaxin-8 (STX8) Syntaxin family Q9UNK0

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
5 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
8 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
9 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587