Details of the Target

General Information of Target

Target ID LDTP01361
Target Name DnaJ homolog subfamily C member 8 (DNAJC8)
Gene Name DNAJC8
Gene ID 22826
Synonyms
SPF31; DnaJ homolog subfamily C member 8; Splicing protein spf31
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAASGESGTSGGGGSTEEAFMTFYSEVKQIEKRDSVLTSKNQIERLTRPGSSYFNLNPFE
VLQIDPEVTDEEIKKRFRQLSILVHPDKNQDDADRAQKAFEAVDKAYKLLLDQEQKKRAL
DVIQAGKEYVEHTVKERKKQLKKEGKPTIVEEDDPELFKQAVYKQTMKLFAELEIKRKER
EAKEMHERKRQREEEIEAQEKAKREREWQKNFEESRDGRVDSWRNFQANTKGKKEKKNRT
FLRPPKVKMEQRE
Target Bioclass
Other
Subcellular location
Nucleus
Function Suppresses polyglutamine (polyQ) aggregation of ATXN3 in neuronal cells.
Uniprot ID
O75937
Ensemble ID
ENST00000263697.6
HGNC ID
HGNC:15470

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		8.36  LDD0066  [1]
TH211
 Probe Info  		Y53(7.48)  LDD0257  [2]
STPyne
 Probe Info  		K105(4.58); K135(8.58); K164(3.77); K231(9.09)  LDD0277  [3]
AZ-9
 Probe Info  		E172(0.10)  LDD2209  [4]
Probe 1
 Probe Info  		Y129(103.05)  LDD3495  [5]
HHS-482
 Probe Info  		Y53(0.95)  LDD0285  [6]
HHS-475
 Probe Info  		Y53(0.83); Y129(0.88)  LDD0264  [7]
HHS-465
 Probe Info  		Y129(4.79); Y53(2.27)  LDD2237  [8]
5E-2FA
 Probe Info  		N.A.  LDD2235  [9]
ATP probe
 Probe Info  		K143(0.00); K135(0.00); K98(0.00); K231(0.00)  LDD0199  [10]
m-APA
 Probe Info  		N.A.  LDD2231  [9]
NHS
 Probe Info  		K176(0.00); K88(0.00); K105(0.00); K135(0.00)  LDD0010  [11]
SF
 Probe Info  		Y53(0.00); Y129(0.00)  LDD0028  [12]
1c-yne
 Probe Info  		K74(0.00); K210(0.00); K146(0.00)  LDD0228  [13]
Acrolein
 Probe Info  		N.A.  LDD0217  [14]
PAL-AfBPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C003
 Probe Info  		14.52  LDD1713  [15]
C041
 Probe Info  		6.19  LDD1741  [15]
C106
 Probe Info  		16.22  LDD1793  [15]
C187
 Probe Info  		15.03  LDD1865  [15]
C191
 Probe Info  		12.47  LDD1868  [15]
C361
 Probe Info  		19.97  LDD2022  [15]
FFF probe13
 Probe Info  		14.22  LDD0475  [16]
JN0003
 Probe Info  		20.00  LDD0469  [16]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [14]
 LDCM0116  HHS-0101 DM93 Y53(0.83); Y129(0.88)  LDD0264  [7]
 LDCM0117  HHS-0201 DM93 Y53(0.85); Y129(1.03)  LDD0265  [7]
 LDCM0118  HHS-0301 DM93 Y129(0.81); Y53(1.17)  LDD0266  [7]
 LDCM0119  HHS-0401 DM93 Y53(0.93); Y129(1.06)  LDD0267  [7]
 LDCM0120  HHS-0701 DM93 Y129(1.02); Y53(1.05)  LDD0268  [7]
 LDCM0107  IAA HeLa N.A.  LDD0221  [14]
 LDCM0123  JWB131 DM93 Y53(0.95)  LDD0285  [6]
 LDCM0124  JWB142 DM93 Y53(1.05)  LDD0286  [6]
 LDCM0125  JWB146 DM93 Y53(1.59)  LDD0287  [6]
 LDCM0126  JWB150 DM93 Y53(2.35)  LDD0288  [6]
 LDCM0127  JWB152 DM93 Y53(1.91)  LDD0289  [6]
 LDCM0128  JWB198 DM93 Y53(4.25)  LDD0290  [6]
 LDCM0129  JWB202 DM93 Y53(1.30)  LDD0291  [6]
 LDCM0130  JWB211 DM93 Y53(1.73)  LDD0292  [6]
 LDCM0109  NEM HeLa N.A.  LDD0223  [14]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Type-1 angiotensin II receptor (AGTR1) G-protein coupled receptor 1 family P30556

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
5 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
6 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
7 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
8 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
9 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
10 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
11 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
12 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
13 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
14 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
15 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
16 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.