Details of the Target

General Information of Target

Target ID LDTP05424
Target Name Keratin, type I cytoskeletal 17 (KRT17)
Gene Name KRT17
Gene ID 3872
Synonyms
Keratin, type I cytoskeletal 17; 39.1; Cytokeratin-17; CK-17; Keratin-17; K17
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGLGAGSCRLGSAGGLGSTLGGSSYSSC
YSFGSGGGYGSSFGGVDGLLAGGEKATMQNLNDRLASYLDKVRALEEANTELEVKIRDWY
QRQAPGPARDYSQYYRTIEELQNKILTATVDNANILLQIDNARLAADDFRTKFETEQALR
LSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNALRGQVGGEINVE
MDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEDWFFSKTEELNREVATNSELVQSGKSEI
SELRRTMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVEEQLAQLRCEM
EQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKKEPVTTRQVRTIVEEVQDGKV
ISSREQVHQTTR
Target Type
Literature-reported
Target Bioclass
Other
Family
Intermediate filament family
Subcellular location
Cytoplasm
Function
Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors. May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.
TTD ID
T07591
Uniprot ID
Q04695
DrugMap ID
TTKV0EC
Ensemble ID
ENST00000311208.13
HGNC ID
HGNC:6427

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 16 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P3
 Probe Info  		5.81  LDD0450  [1]
P8
 Probe Info  		1.98  LDD0451  [1]
AZ-5
 Probe Info  		5.04  LDD0394  [2]
YN-1
 Probe Info  		100.00  LDD0444  [3]
DBIA
 Probe Info  		C336(4.59); C40(2.42)  LDD3313  [4]
Jackson_14
 Probe Info  		8.33  LDD0123  [5]
W1
 Probe Info  		C40(1.30)  LDD0239  [6]
m-APA
 Probe Info  		H428(0.00); H394(0.00)  LDD2231  [7]
IA-alkyne
 Probe Info  		N.A.  LDD0149  [8]
IPM
 Probe Info  		C40(0.00); C358(0.00)  LDD0147  [9]
TFBX
 Probe Info  		N.A.  LDD0148  [9]
Acrolein
 Probe Info  		H394(0.00); H222(0.00); C40(0.00); C358(0.00)  LDD0217  [10]
Cinnamaldehyde
 Probe Info  		C40(0.00); C358(0.00)  LDD0220  [10]
Crotonaldehyde
 Probe Info  		C40(0.00); C358(0.00); H428(0.00); H394(0.00)  LDD0219  [10]
Methacrolein
 Probe Info  		H222(0.00); H394(0.00); C358(0.00); C40(0.00)  LDD0218  [10]
HHS-465
 Probe Info  		Y398(0.00); Y69(0.00); Y61(0.00); K399(0.00)  LDD2240  [11]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		1.14  LDD0136  [12]
STS-2
 Probe Info  		1.05  LDD0138  [12]
Dasatinib-CA-8PAP
 Probe Info  		N.A.  LDD0364  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H222(0.00); C40(0.00); C358(0.00); H394(0.00)  LDD0222  [10]
 LDCM0097  Dasatinib K562 N.A.  LDD0364  [13]
 LDCM0107  IAA HeLa H394(0.00); H222(0.00); C40(0.00); H428(0.00)  LDD0221  [10]
 LDCM0022  KB02 8305C C389(1.89); C40(2.16)  LDD2248  [4]
 LDCM0023  KB03 8305C C389(4.48)  LDD2665  [4]
 LDCM0024  KB05 Hs 936.T C336(4.59); C40(2.42)  LDD3313  [4]
 LDCM0109  NEM HeLa H394(0.00); H222(0.00); C40(0.00); H428(0.00)  LDD0223  [10]
 LDCM0016  Ranjitkar_cp1 MDA-MB-231 8.33  LDD0123  [5]
 LDCM0112  W16 Hep-G2 C40(1.30)  LDD0239  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase TRIM69 (TRIM69) TRIM/RBCC family Q86WT6
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Coiled-coil domain-containing protein 85B (CCDC85B) CCDC85 family Q15834
Elongation factor 1-gamma (EEF1G) . P26641

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
3 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
6 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning. Biochemistry. 2018 Jan 30;57(4):451-460. doi: 10.1021/acs.biochem.7b00897. Epub 2017 Oct 26.
9 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
10 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
11 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
12 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
13 Streamlined Target Deconvolution Approach Utilizing a Single Photoreactive Chloroalkane Capture Tag. ACS Chem Biol. 2021 Feb 19;16(2):404-413. doi: 10.1021/acschembio.0c00987. Epub 2021 Feb 5.