Details of the Target

General Information of Target

Target ID LDTP03618
Target Name 14-3-3 protein sigma (SFN)
Gene Name SFN
Gene ID 2810
Synonyms
HME1; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWR
VLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAESRVFY
LKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFH
YEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGG
EAPQEPQS
Target Bioclass
Other
Family
14-3-3 family
Subcellular location
Cytoplasm
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response. Also acts as a TP53/p53-regulated inhibitor of G2/M progression. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
Uniprot ID
P31947
Ensemble ID
ENST00000339276.6
HGNC ID
HGNC:10773
ChEMBL ID
CHEMBL1909482

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CHL1 SNV: p.A135T; p.G137D .
Ishikawa (Heraklio) 02 ER SNV: p.Y181H DBIA    Probe Info 
MOLT4 SNV: p.R82C; p.D126N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 33 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P1
 Probe Info  		4.62  LDD0448  [1]
P2
 Probe Info  		8.09  LDD0449  [1]
P3
 Probe Info  		10.00  LDD0450  [1]
P8
 Probe Info  		5.48  LDD0451  [1]
AZ-9
 Probe Info  		2.30  LDD0393  [2]
CY-1
 Probe Info  		4.50  LDD0243  [3]
YN-1
 Probe Info  		100.00  LDD0444  [4]
ONAyne
 Probe Info  		K32(5.00); K68(8.06); K9(1.31)  LDD0274  [5]
STPyne
 Probe Info  		K140(8.75); K141(0.34); K159(1.28); K49(5.61)  LDD0277  [5]
BTD
 Probe Info  		C38(8.91)  LDD1699  [6]
OPA-S-S-alkyne
 Probe Info  		K77(3.35); K124(4.42); K49(5.33)  LDD3494  [7]
Probe 1
 Probe Info  		Y19(66.73); Y130(109.22); Y213(29.67)  LDD3495  [8]
JZ128-DTB
 Probe Info  		N.A.  LDD0462  [9]
THZ1-DTB
 Probe Info  		C38(1.04)  LDD0460  [9]
AHL-Pu-1
 Probe Info  		C38(2.89)  LDD0169  [10]
HHS-465
 Probe Info  		Y127(2.82); Y128(1.47); Y48(10.00)  LDD2237  [11]
DBIA
 Probe Info  		C38(0.98)  LDD0078  [12]
m-APA
 Probe Info  		H106(0.00); H206(0.00)  LDD2231  [13]
ATP probe
 Probe Info  		K11(0.00); K49(0.00); K124(0.00); K140(0.00)  LDD0035  [14]
IPM
 Probe Info  		N.A.  LDD0025  [15]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [15]
NAIA_4
 Probe Info  		N.A.  LDD2226  [16]
TFBX
 Probe Info  		N.A.  LDD0027  [15]
IA-alkyne
 Probe Info  		N.A.  LDD0149  [17]
PF-06672131
 Probe Info  		N.A.  LDD0017  [18]
Acrolein
 Probe Info  		C38(0.00); H206(0.00)  LDD0217  [19]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [19]
Methacrolein
 Probe Info  		N.A.  LDD0218  [19]
W1
 Probe Info  		N.A.  LDD0236  [20]
MPP-AC
 Probe Info  		N.A.  LDD0428  [21]
NAIA_5
 Probe Info  		C96(0.00); C38(0.00)  LDD2223  [16]
HHS-475
 Probe Info  		Y213(1.54)  LDD2238  [11]
HHS-482
 Probe Info  		Y213(1.11)  LDD2239  [11]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DA-2
 Probe Info  		N.A.  LDD0070  [22]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C38(0.89)  LDD2142  [6]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C38(1.20)  LDD2112  [6]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C38(1.09)  LDD2095  [6]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C38(1.11)  LDD2117  [6]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C38(1.08)  LDD2152  [6]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C38(0.98)  LDD2103  [6]
 LDCM0026  4SU-RNA+native RNA HEK-293T C38(2.89)  LDD0169  [10]
 LDCM0214  AC1 HCT 116 C38(1.27)  LDD0531  [12]
 LDCM0215  AC10 HCT 116 C38(1.34)  LDD0532  [12]
 LDCM0216  AC100 HCT 116 C38(1.23)  LDD0533  [12]
 LDCM0217  AC101 HCT 116 C38(1.14)  LDD0534  [12]
 LDCM0218  AC102 HCT 116 C38(1.49)  LDD0535  [12]
 LDCM0219  AC103 HCT 116 C38(1.28)  LDD0536  [12]
 LDCM0220  AC104 HCT 116 C38(1.46)  LDD0537  [12]
 LDCM0221  AC105 HCT 116 C38(1.41)  LDD0538  [12]
 LDCM0222  AC106 HCT 116 C38(1.24)  LDD0539  [12]
 LDCM0223  AC107 HCT 116 C38(1.48)  LDD0540  [12]
 LDCM0224  AC108 HCT 116 C38(1.18)  LDD0541  [12]
 LDCM0225  AC109 HCT 116 C38(0.88)  LDD0542  [12]
 LDCM0226  AC11 HCT 116 C38(1.37)  LDD0543  [12]
 LDCM0227  AC110 HCT 116 C38(1.20)  LDD0544  [12]
 LDCM0228  AC111 HCT 116 C38(1.12)  LDD0545  [12]
 LDCM0229  AC112 HCT 116 C38(1.33)  LDD0546  [12]
 LDCM0230  AC113 HCT 116 C38(1.03)  LDD0547  [12]
 LDCM0231  AC114 HCT 116 C38(1.12)  LDD0548  [12]
 LDCM0232  AC115 HCT 116 C38(1.22)  LDD0549  [12]
 LDCM0233  AC116 HCT 116 C38(1.29)  LDD0550  [12]
 LDCM0234  AC117 HCT 116 C38(1.26)  LDD0551  [12]
 LDCM0235  AC118 HCT 116 C38(1.19)  LDD0552  [12]
 LDCM0236  AC119 HCT 116 C38(1.43)  LDD0553  [12]
 LDCM0237  AC12 HCT 116 C38(1.23)  LDD0554  [12]
 LDCM0238  AC120 HCT 116 C38(1.31)  LDD0555  [12]
 LDCM0239  AC121 HCT 116 C38(1.12)  LDD0556  [12]
 LDCM0240  AC122 HCT 116 C38(1.25)  LDD0557  [12]
 LDCM0241  AC123 HCT 116 C38(1.02)  LDD0558  [12]
 LDCM0242  AC124 HCT 116 C38(1.00)  LDD0559  [12]
 LDCM0243  AC125 HCT 116 C38(1.16)  LDD0560  [12]
 LDCM0244  AC126 HCT 116 C38(1.16)  LDD0561  [12]
 LDCM0245  AC127 HCT 116 C38(1.16)  LDD0562  [12]
 LDCM0246  AC128 HCT 116 C38(1.22)  LDD0563  [12]
 LDCM0247  AC129 HCT 116 C38(1.15)  LDD0564  [12]
 LDCM0249  AC130 HCT 116 C38(1.25)  LDD0566  [12]
 LDCM0250  AC131 HCT 116 C38(0.92)  LDD0567  [12]
 LDCM0251  AC132 HCT 116 C38(1.14)  LDD0568  [12]
 LDCM0252  AC133 HCT 116 C38(1.22)  LDD0569  [12]
 LDCM0253  AC134 HCT 116 C38(1.25)  LDD0570  [12]
 LDCM0254  AC135 HCT 116 C38(1.15)  LDD0571  [12]
 LDCM0255  AC136 HCT 116 C38(1.19)  LDD0572  [12]
 LDCM0256  AC137 HCT 116 C38(1.40)  LDD0573  [12]
 LDCM0257  AC138 HCT 116 C38(1.57)  LDD0574  [12]
 LDCM0258  AC139 HCT 116 C38(1.36)  LDD0575  [12]
 LDCM0259  AC14 HCT 116 C38(1.15)  LDD0576  [12]
 LDCM0260  AC140 HCT 116 C38(1.30)  LDD0577  [12]
 LDCM0261  AC141 HCT 116 C38(1.46)  LDD0578  [12]
 LDCM0262  AC142 HCT 116 C38(1.13)  LDD0579  [12]
 LDCM0263  AC143 HCT 116 C38(1.06)  LDD0580  [12]
 LDCM0264  AC144 HCT 116 C38(1.02)  LDD0581  [12]
 LDCM0265  AC145 HCT 116 C38(0.95)  LDD0582  [12]
 LDCM0266  AC146 HCT 116 C38(1.38)  LDD0583  [12]
 LDCM0267  AC147 HCT 116 C38(1.11)  LDD0584  [12]
 LDCM0268  AC148 HCT 116 C38(1.82)  LDD0585  [12]
 LDCM0269  AC149 HCT 116 C38(1.39)  LDD0586  [12]
 LDCM0270  AC15 HCT 116 C38(1.16)  LDD0587  [12]
 LDCM0271  AC150 HCT 116 C38(1.17)  LDD0588  [12]
 LDCM0272  AC151 HCT 116 C38(1.06)  LDD0589  [12]
 LDCM0273  AC152 HCT 116 C38(1.12)  LDD0590  [12]
 LDCM0274  AC153 HCT 116 C38(1.77)  LDD0591  [12]
 LDCM0621  AC154 HCT 116 C38(1.04)  LDD2158  [12]
 LDCM0622  AC155 HCT 116 C38(1.03)  LDD2159  [12]
 LDCM0623  AC156 HCT 116 C38(1.17)  LDD2160  [12]
 LDCM0624  AC157 HCT 116 C38(0.90)  LDD2161  [12]
 LDCM0276  AC17 HCT 116 C38(1.24)  LDD0593  [12]
 LDCM0277  AC18 HCT 116 C38(1.28)  LDD0594  [12]
 LDCM0278  AC19 HCT 116 C38(1.21)  LDD0595  [12]
 LDCM0279  AC2 HCT 116 C38(1.06)  LDD0596  [12]
 LDCM0280  AC20 HCT 116 C38(1.39)  LDD0597  [12]
 LDCM0281  AC21 HCT 116 C38(1.49)  LDD0598  [12]
 LDCM0282  AC22 HCT 116 C38(1.23)  LDD0599  [12]
 LDCM0283  AC23 HCT 116 C38(1.36)  LDD0600  [12]
 LDCM0284  AC24 HCT 116 C38(1.20)  LDD0601  [12]
 LDCM0285  AC25 HCT 116 C38(1.08)  LDD0602  [12]
 LDCM0286  AC26 HCT 116 C38(1.29)  LDD0603  [12]
 LDCM0287  AC27 HCT 116 C38(1.21)  LDD0604  [12]
 LDCM0288  AC28 HCT 116 C38(1.28)  LDD0605  [12]
 LDCM0289  AC29 HCT 116 C38(1.18)  LDD0606  [12]
 LDCM0290  AC3 HCT 116 C38(1.10)  LDD0607  [12]
 LDCM0291  AC30 HCT 116 C38(1.27)  LDD0608  [12]
 LDCM0292  AC31 HCT 116 C38(1.17)  LDD0609  [12]
 LDCM0293  AC32 HCT 116 C38(1.57)  LDD0610  [12]
 LDCM0294  AC33 HCT 116 C38(1.32)  LDD0611  [12]
 LDCM0295  AC34 HCT 116 C38(1.52)  LDD0612  [12]
 LDCM0296  AC35 HCT 116 C38(0.97)  LDD0613  [12]
 LDCM0297  AC36 HCT 116 C38(1.01)  LDD0614  [12]
 LDCM0298  AC37 HCT 116 C38(1.04)  LDD0615  [12]
 LDCM0299  AC38 HCT 116 C38(1.07)  LDD0616  [12]
 LDCM0300  AC39 HCT 116 C38(1.29)  LDD0617  [12]
 LDCM0301  AC4 HCT 116 C38(1.19)  LDD0618  [12]
 LDCM0302  AC40 HCT 116 C38(1.39)  LDD0619  [12]
 LDCM0303  AC41 HCT 116 C38(1.25)  LDD0620  [12]
 LDCM0304  AC42 HCT 116 C38(1.15)  LDD0621  [12]
 LDCM0305  AC43 HCT 116 C38(1.26)  LDD0622  [12]
 LDCM0306  AC44 HCT 116 C38(1.23)  LDD0623  [12]
 LDCM0307  AC45 HCT 116 C38(1.34)  LDD0624  [12]
 LDCM0308  AC46 HCT 116 C38(1.18)  LDD0625  [12]
 LDCM0309  AC47 HCT 116 C38(1.11)  LDD0626  [12]
 LDCM0310  AC48 HCT 116 C38(1.38)  LDD0627  [12]
 LDCM0311  AC49 HCT 116 C38(1.67)  LDD0628  [12]
 LDCM0312  AC5 HCT 116 C38(1.30)  LDD0629  [12]
 LDCM0313  AC50 HCT 116 C38(1.44)  LDD0630  [12]
 LDCM0314  AC51 HCT 116 C38(1.09)  LDD0631  [12]
 LDCM0315  AC52 HCT 116 C38(1.18)  LDD0632  [12]
 LDCM0316  AC53 HCT 116 C38(1.20)  LDD0633  [12]
 LDCM0317  AC54 HCT 116 C38(1.34)  LDD0634  [12]
 LDCM0318  AC55 HCT 116 C38(1.50)  LDD0635  [12]
 LDCM0319  AC56 HCT 116 C38(2.40)  LDD0636  [12]
 LDCM0320  AC57 HCT 116 C38(1.51)  LDD0637  [12]
 LDCM0321  AC58 HCT 116 C38(1.68)  LDD0638  [12]
 LDCM0322  AC59 HCT 116 C38(1.47)  LDD0639  [12]
 LDCM0323  AC6 HCT 116 C38(1.12)  LDD0640  [12]
 LDCM0324  AC60 HCT 116 C38(1.55)  LDD0641  [12]
 LDCM0325  AC61 HCT 116 C38(1.40)  LDD0642  [12]
 LDCM0326  AC62 HCT 116 C38(1.63)  LDD0643  [12]
 LDCM0327  AC63 HCT 116 C38(1.51)  LDD0644  [12]
 LDCM0328  AC64 HCT 116 C38(1.66)  LDD0645  [12]
 LDCM0329  AC65 HCT 116 C38(1.68)  LDD0646  [12]
 LDCM0330  AC66 HCT 116 C38(1.86)  LDD0647  [12]
 LDCM0331  AC67 HCT 116 C38(2.18)  LDD0648  [12]
 LDCM0332  AC68 HCT 116 C38(1.10)  LDD0649  [12]
 LDCM0333  AC69 HCT 116 C38(1.38)  LDD0650  [12]
 LDCM0334  AC7 HCT 116 C38(1.20)  LDD0651  [12]
 LDCM0335  AC70 HCT 116 C38(1.31)  LDD0652  [12]
 LDCM0336  AC71 HCT 116 C38(1.12)  LDD0653  [12]
 LDCM0337  AC72 HCT 116 C38(1.14)  LDD0654  [12]
 LDCM0338  AC73 HCT 116 C38(1.64)  LDD0655  [12]
 LDCM0339  AC74 HCT 116 C38(1.55)  LDD0656  [12]
 LDCM0340  AC75 HCT 116 C38(1.94)  LDD0657  [12]
 LDCM0341  AC76 HCT 116 C38(1.35)  LDD0658  [12]
 LDCM0342  AC77 HCT 116 C38(1.25)  LDD0659  [12]
 LDCM0343  AC78 HCT 116 C38(1.22)  LDD0660  [12]
 LDCM0344  AC79 HCT 116 C38(1.06)  LDD0661  [12]
 LDCM0345  AC8 HCT 116 C38(1.33)  LDD0662  [12]
 LDCM0346  AC80 HCT 116 C38(1.02)  LDD0663  [12]
 LDCM0347  AC81 HCT 116 C38(1.30)  LDD0664  [12]
 LDCM0348  AC82 HCT 116 C38(2.07)  LDD0665  [12]
 LDCM0349  AC83 HCT 116 C38(1.61)  LDD0666  [12]
 LDCM0350  AC84 HCT 116 C38(1.97)  LDD0667  [12]
 LDCM0351  AC85 HCT 116 C38(1.68)  LDD0668  [12]
 LDCM0352  AC86 HCT 116 C38(1.10)  LDD0669  [12]
 LDCM0353  AC87 HCT 116 C38(1.09)  LDD0670  [12]
 LDCM0354  AC88 HCT 116 C38(1.16)  LDD0671  [12]
 LDCM0355  AC89 HCT 116 C38(1.38)  LDD0672  [12]
 LDCM0357  AC90 HCT 116 C38(1.06)  LDD0674  [12]
 LDCM0358  AC91 HCT 116 C38(2.24)  LDD0675  [12]
 LDCM0359  AC92 HCT 116 C38(1.44)  LDD0676  [12]
 LDCM0360  AC93 HCT 116 C38(1.37)  LDD0677  [12]
 LDCM0361  AC94 HCT 116 C38(1.09)  LDD0678  [12]
 LDCM0362  AC95 HCT 116 C38(1.18)  LDD0679  [12]
 LDCM0363  AC96 HCT 116 C38(1.42)  LDD0680  [12]
 LDCM0364  AC97 HCT 116 C38(1.44)  LDD0681  [12]
 LDCM0365  AC98 HCT 116 C38(2.24)  LDD0682  [12]
 LDCM0366  AC99 HCT 116 C38(1.25)  LDD0683  [12]
 LDCM0248  AKOS034007472 HCT 116 C38(0.97)  LDD0565  [12]
 LDCM0356  AKOS034007680 HCT 116 C38(1.08)  LDD0673  [12]
 LDCM0275  AKOS034007705 HCT 116 C38(1.74)  LDD0592  [12]
 LDCM0020  ARS-1620 HCC44 C38(0.98)  LDD0078  [12]
 LDCM0630  CCW28-3 231MFP C38(1.16); C96(1.12)  LDD2214  [23]
 LDCM0108  Chloroacetamide HeLa C38(0.00); H206(0.00)  LDD0222  [19]
 LDCM0632  CL-Sc Hep-G2 C38(1.07); C38(1.05); C38(0.36)  LDD2227  [16]
 LDCM0367  CL1 HCT 116 C38(1.01)  LDD0684  [12]
 LDCM0368  CL10 HCT 116 C38(1.18)  LDD0685  [12]
 LDCM0369  CL100 HCT 116 C38(1.24)  LDD0686  [12]
 LDCM0370  CL101 HCT 116 C38(1.22)  LDD0687  [12]
 LDCM0371  CL102 HCT 116 C38(1.13)  LDD0688  [12]
 LDCM0372  CL103 HCT 116 C38(1.14)  LDD0689  [12]
 LDCM0373  CL104 HCT 116 C38(1.05)  LDD0690  [12]
 LDCM0374  CL105 HCT 116 C38(1.37)  LDD0691  [12]
 LDCM0375  CL106 HCT 116 C38(1.33)  LDD0692  [12]
 LDCM0376  CL107 HCT 116 C38(1.27)  LDD0693  [12]
 LDCM0377  CL108 HCT 116 C38(1.10)  LDD0694  [12]
 LDCM0378  CL109 HCT 116 C38(1.38)  LDD0695  [12]
 LDCM0379  CL11 HCT 116 C38(1.37)  LDD0696  [12]
 LDCM0380  CL110 HCT 116 C38(1.10)  LDD0697  [12]
 LDCM0381  CL111 HCT 116 C38(1.35)  LDD0698  [12]
 LDCM0382  CL112 HCT 116 C38(1.11)  LDD0699  [12]
 LDCM0383  CL113 HCT 116 C38(1.42)  LDD0700  [12]
 LDCM0384  CL114 HCT 116 C38(1.77)  LDD0701  [12]
 LDCM0385  CL115 HCT 116 C38(1.23)  LDD0702  [12]
 LDCM0386  CL116 HCT 116 C38(1.24)  LDD0703  [12]
 LDCM0387  CL117 HCT 116 C38(1.83)  LDD0704  [12]
 LDCM0388  CL118 HCT 116 C38(1.22)  LDD0705  [12]
 LDCM0389  CL119 HCT 116 C38(1.29)  LDD0706  [12]
 LDCM0390  CL12 HCT 116 C38(1.33)  LDD0707  [12]
 LDCM0391  CL120 HCT 116 C38(1.24)  LDD0708  [12]
 LDCM0392  CL121 HCT 116 C38(1.17)  LDD0709  [12]
 LDCM0393  CL122 HCT 116 C38(1.23)  LDD0710  [12]
 LDCM0394  CL123 HCT 116 C38(1.20)  LDD0711  [12]
 LDCM0395  CL124 HCT 116 C38(1.66)  LDD0712  [12]
 LDCM0396  CL125 HCT 116 C38(1.19)  LDD0713  [12]
 LDCM0397  CL126 HCT 116 C38(1.37)  LDD0714  [12]
 LDCM0398  CL127 HCT 116 C38(1.35)  LDD0715  [12]
 LDCM0399  CL128 HCT 116 C38(1.41)  LDD0716  [12]
 LDCM0400  CL13 HCT 116 C38(1.13)  LDD0717  [12]
 LDCM0401  CL14 HCT 116 C38(0.97)  LDD0718  [12]
 LDCM0402  CL15 HCT 116 C38(1.10)  LDD0719  [12]
 LDCM0403  CL16 HCT 116 C38(1.09)  LDD0720  [12]
 LDCM0404  CL17 HCT 116 C38(1.18)  LDD0721  [12]
 LDCM0405  CL18 HCT 116 C38(1.16)  LDD0722  [12]
 LDCM0406  CL19 HCT 116 C38(1.07)  LDD0723  [12]
 LDCM0407  CL2 HCT 116 C38(0.95)  LDD0724  [12]
 LDCM0408  CL20 HCT 116 C38(1.13)  LDD0725  [12]
 LDCM0409  CL21 HCT 116 C38(1.37)  LDD0726  [12]
 LDCM0410  CL22 HCT 116 C38(2.26)  LDD0727  [12]
 LDCM0411  CL23 HCT 116 C38(1.18)  LDD0728  [12]
 LDCM0412  CL24 HCT 116 C38(1.27)  LDD0729  [12]
 LDCM0413  CL25 HCT 116 C38(1.52)  LDD0730  [12]
 LDCM0414  CL26 HCT 116 C38(1.18)  LDD0731  [12]
 LDCM0415  CL27 HCT 116 C38(1.07)  LDD0732  [12]
 LDCM0416  CL28 HCT 116 C38(1.29)  LDD0733  [12]
 LDCM0417  CL29 HCT 116 C38(1.23)  LDD0734  [12]
 LDCM0418  CL3 HCT 116 C38(1.07)  LDD0735  [12]
 LDCM0419  CL30 HCT 116 C38(1.05)  LDD0736  [12]
 LDCM0420  CL31 HCT 116 C38(1.10)  LDD0737  [12]
 LDCM0421  CL32 HCT 116 C38(1.37)  LDD0738  [12]
 LDCM0422  CL33 HCT 116 C38(1.63)  LDD0739  [12]
 LDCM0423  CL34 HCT 116 C38(0.78)  LDD0740  [12]
 LDCM0424  CL35 HCT 116 C38(0.84)  LDD0741  [12]
 LDCM0425  CL36 HCT 116 C38(0.75)  LDD0742  [12]
 LDCM0426  CL37 HCT 116 C38(0.72)  LDD0743  [12]
 LDCM0428  CL39 HCT 116 C38(0.79)  LDD0745  [12]
 LDCM0429  CL4 HCT 116 C38(1.38)  LDD0746  [12]
 LDCM0430  CL40 HCT 116 C38(0.80)  LDD0747  [12]
 LDCM0431  CL41 HCT 116 C38(0.87)  LDD0748  [12]
 LDCM0432  CL42 HCT 116 C38(0.90)  LDD0749  [12]
 LDCM0433  CL43 HCT 116 C38(0.99)  LDD0750  [12]
 LDCM0434  CL44 HCT 116 C38(0.83)  LDD0751  [12]
 LDCM0435  CL45 HCT 116 C38(0.85)  LDD0752  [12]
 LDCM0436  CL46 HCT 116 C38(1.20)  LDD0753  [12]
 LDCM0437  CL47 HCT 116 C38(1.09)  LDD0754  [12]
 LDCM0438  CL48 HCT 116 C38(1.21)  LDD0755  [12]
 LDCM0439  CL49 HCT 116 C38(1.08)  LDD0756  [12]
 LDCM0440  CL5 HCT 116 C38(0.96)  LDD0757  [12]
 LDCM0441  CL50 HCT 116 C38(1.15)  LDD0758  [12]
 LDCM0442  CL51 HCT 116 C38(1.24)  LDD0759  [12]
 LDCM0443  CL52 HCT 116 C38(1.19)  LDD0760  [12]
 LDCM0444  CL53 HCT 116 C38(1.04)  LDD0761  [12]
 LDCM0445  CL54 HCT 116 C38(1.27)  LDD0762  [12]
 LDCM0446  CL55 HCT 116 C38(1.08)  LDD0763  [12]
 LDCM0447  CL56 HCT 116 C38(1.21)  LDD0764  [12]
 LDCM0448  CL57 HCT 116 C38(0.95)  LDD0765  [12]
 LDCM0449  CL58 HCT 116 C38(1.13)  LDD0766  [12]
 LDCM0450  CL59 HCT 116 C38(1.06)  LDD0767  [12]
 LDCM0451  CL6 HCT 116 C38(1.14)  LDD0768  [12]
 LDCM0452  CL60 HCT 116 C38(1.09)  LDD0769  [12]
 LDCM0453  CL61 HCT 116 C38(1.17)  LDD0770  [12]
 LDCM0454  CL62 HCT 116 C38(1.30)  LDD0771  [12]
 LDCM0455  CL63 HCT 116 C38(1.48)  LDD0772  [12]
 LDCM0456  CL64 HCT 116 C38(1.38)  LDD0773  [12]
 LDCM0457  CL65 HCT 116 C38(1.36)  LDD0774  [12]
 LDCM0458  CL66 HCT 116 C38(1.79)  LDD0775  [12]
 LDCM0459  CL67 HCT 116 C38(1.36)  LDD0776  [12]
 LDCM0460  CL68 HCT 116 C38(1.26)  LDD0777  [12]
 LDCM0461  CL69 HCT 116 C38(1.38)  LDD0778  [12]
 LDCM0462  CL7 HCT 116 C38(1.27)  LDD0779  [12]
 LDCM0463  CL70 HCT 116 C38(1.18)  LDD0780  [12]
 LDCM0464  CL71 HCT 116 C38(1.31)  LDD0781  [12]
 LDCM0465  CL72 HCT 116 C38(1.17)  LDD0782  [12]
 LDCM0466  CL73 HCT 116 C38(1.18)  LDD0783  [12]
 LDCM0467  CL74 HCT 116 C38(1.21)  LDD0784  [12]
 LDCM0469  CL76 HCT 116 C38(1.22)  LDD0786  [12]
 LDCM0470  CL77 HCT 116 C38(1.16)  LDD0787  [12]
 LDCM0471  CL78 HCT 116 C38(1.28)  LDD0788  [12]
 LDCM0472  CL79 HCT 116 C38(1.33)  LDD0789  [12]
 LDCM0473  CL8 HCT 116 C38(1.65)  LDD0790  [12]
 LDCM0474  CL80 HCT 116 C38(1.18)  LDD0791  [12]
 LDCM0475  CL81 HCT 116 C38(1.23)  LDD0792  [12]
 LDCM0476  CL82 HCT 116 C38(1.64)  LDD0793  [12]
 LDCM0477  CL83 HCT 116 C38(1.63)  LDD0794  [12]
 LDCM0478  CL84 HCT 116 C38(2.15)  LDD0795  [12]
 LDCM0479  CL85 HCT 116 C38(1.33)  LDD0796  [12]
 LDCM0480  CL86 HCT 116 C38(1.23)  LDD0797  [12]
 LDCM0481  CL87 HCT 116 C38(1.31)  LDD0798  [12]
 LDCM0482  CL88 HCT 116 C38(1.42)  LDD0799  [12]
 LDCM0483  CL89 HCT 116 C38(2.64)  LDD0800  [12]
 LDCM0484  CL9 HCT 116 C38(1.15)  LDD0801  [12]
 LDCM0485  CL90 HCT 116 C38(1.38)  LDD0802  [12]
 LDCM0486  CL91 HCT 116 C38(1.31)  LDD0803  [12]
 LDCM0487  CL92 HCT 116 C38(1.24)  LDD0804  [12]
 LDCM0488  CL93 HCT 116 C38(1.21)  LDD0805  [12]
 LDCM0489  CL94 HCT 116 C38(1.30)  LDD0806  [12]
 LDCM0490  CL95 HCT 116 C38(1.52)  LDD0807  [12]
 LDCM0491  CL96 HCT 116 C38(1.20)  LDD0808  [12]
 LDCM0492  CL97 HCT 116 C38(1.19)  LDD0809  [12]
 LDCM0493  CL98 HCT 116 C38(1.25)  LDD0810  [12]
 LDCM0494  CL99 HCT 116 C38(1.32)  LDD0811  [12]
 LDCM0634  CY-0357 Hep-G2 C38(1.18); C38(0.95)  LDD2228  [16]
 LDCM0625  F8 Ramos C38(1.32)  LDD2187  [24]
 LDCM0572  Fragment10 Ramos C38(1.20)  LDD2189  [24]
 LDCM0574  Fragment12 Ramos C38(0.63)  LDD2191  [24]
 LDCM0575  Fragment13 Ramos C38(0.80)  LDD2192  [24]
 LDCM0576  Fragment14 Ramos C38(0.81)  LDD2193  [24]
 LDCM0579  Fragment20 Ramos C38(0.97)  LDD2194  [24]
 LDCM0580  Fragment21 Ramos C38(0.78)  LDD2195  [24]
 LDCM0582  Fragment23 Ramos C38(0.61)  LDD2196  [24]
 LDCM0578  Fragment27 Ramos C38(0.66)  LDD2197  [24]
 LDCM0588  Fragment30 Ramos C38(0.98)  LDD2199  [24]
 LDCM0589  Fragment31 Ramos C38(0.54)  LDD2200  [24]
 LDCM0590  Fragment32 Ramos C38(1.29)  LDD2201  [24]
 LDCM0468  Fragment33 HCT 116 C38(1.27)  LDD0785  [12]
 LDCM0596  Fragment38 Ramos C38(0.41)  LDD2203  [24]
 LDCM0566  Fragment4 Ramos C38(0.59)  LDD2184  [24]
 LDCM0427  Fragment51 HCT 116 C38(0.70)  LDD0744  [12]
 LDCM0610  Fragment52 Ramos C38(0.84)  LDD2204  [24]
 LDCM0614  Fragment56 Ramos C38(0.74)  LDD2205  [24]
 LDCM0569  Fragment7 Ramos C38(0.80)  LDD2186  [24]
 LDCM0571  Fragment9 Ramos C38(1.05)  LDD2188  [24]
 LDCM0107  IAA HeLa N.A.  LDD0221  [19]
 LDCM0179  JZ128 PC-3 N.A.  LDD0462  [9]
 LDCM0022  KB02 Ramos C38(1.82)  LDD2182  [24]
 LDCM0023  KB03 Jurkat C38(4.16)  LDD0209  [25]
 LDCM0024  KB05 HMCB C96(1.55)  LDD3312  [26]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C38(0.96)  LDD2102  [6]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C38(0.89)  LDD2121  [6]
 LDCM0109  NEM HeLa N.A.  LDD0223  [19]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C38(1.02)  LDD2089  [6]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C38(1.23)  LDD2090  [6]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C38(1.06)  LDD2092  [6]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C38(1.04)  LDD2094  [6]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C38(0.95)  LDD2098  [6]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C38(1.03)  LDD2099  [6]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C38(1.16)  LDD2100  [6]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C38(0.97)  LDD2104  [6]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C38(1.06)  LDD2105  [6]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C38(0.87)  LDD2106  [6]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C38(0.99)  LDD2108  [6]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C38(1.08)  LDD2109  [6]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C38(1.90)  LDD2110  [6]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C38(0.65)  LDD2114  [6]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C38(0.88)  LDD2116  [6]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C38(0.19)  LDD2118  [6]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C38(0.83)  LDD2120  [6]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C38(1.20)  LDD2124  [6]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C38(1.07)  LDD2125  [6]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C38(0.89)  LDD2126  [6]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C38(1.40)  LDD2128  [6]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C38(0.89)  LDD2136  [6]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C38(0.92)  LDD2137  [6]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C38(0.87)  LDD2140  [6]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C38(1.75)  LDD2141  [6]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C38(1.04)  LDD2150  [6]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C38(0.22)  LDD2151  [6]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C38(1.72)  LDD2153  [6]
 LDCM0627  NUDT7-COV-1 HEK-293T C38(1.04)  LDD2206  [27]
 LDCM0131  RA190 MM1.R C38(1.03)  LDD0304  [28]
 LDCM0021  THZ1 HeLa S3 C38(1.04)  LDD0460  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 18 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase COP1 (COP1) COP1 family Q8NHY2
Histone deacetylase 4 (HDAC4) Histone deacetylase family P56524
Histone deacetylase 5 (HDAC5) Histone deacetylase family Q9UQL6
Histone deacetylase 7 (HDAC7) Histone deacetylase family Q8WUI4
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
COP9 signalosome complex subunit 6 (COPS6) Peptidase M67A family Q7L5N1
MAP/microtubule affinity-regulating kinase 3 (MARK3) CAMK Ser/Thr protein kinase family P27448
Serine/threonine-protein kinase PLK4 (PLK4) Ser/Thr protein kinase family O00444
Mitogen-activated protein kinase kinase kinase 2 (MAP3K2) STE Ser/Thr protein kinase family Q9Y2U5
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2) TKL Ser/Thr protein kinase family Q5S007
RAF proto-oncogene serine/threonine-protein kinase (RAF1) TKL Ser/Thr protein kinase family P04049
Serine/threonine-protein kinase A-Raf (ARAF) TKL Ser/Thr protein kinase family P10398
Serine/threonine-protein kinase B-raf (BRAF) TKL Ser/Thr protein kinase family P15056
Non-receptor tyrosine-protein kinase TNK1 (TNK1) Tyr protein kinase family Q13470
Tyrosine-protein kinase ABL1 (ABL1) Tyr protein kinase family P00519
Epidermal growth factor receptor (EGFR) Tyr protein kinase family P00533
LON peptidase N-terminal domain and RING finger protein 1 (LONRF1) . Q17RB8
Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) . Q13064
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein gamma (YWHAG) 14-3-3 family P61981
14-3-3 protein zeta/delta (YWHAZ) 14-3-3 family P63104
Cellular tumor antigen p53 (TP53) P53 family P04637
Transcription factor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Forkhead box protein O4 (FOXO4) . P98177
Paired box protein Pax-9 (PAX9) . P55771
Transcription factor HIVEP3 (HIVEP3) . Q5T1R4
Other
Click To Hide/Show 15 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein sigma (SFN) 14-3-3 family P31947
Bcl2-associated agonist of cell death (BAD) Bcl-2 family Q92934
Cyclin-H (CCNH) Cyclin family P51946
Protein FAM53C (FAM53C) FAM53 family Q9NYF3
Protein FAM9B (FAM9B) FAM9 family Q8IZU0
Microtubule-associated protein 1A (MAP1A) MAP1 family P78559
ERBB receptor feedback inhibitor 1 (ERRFI1) MIG6 family Q9UJM3
Rab-3A-interacting protein (RAB3IP) SEC2 family Q96QF0
Zinc finger C2HC domain-containing protein 1C (ZC2HC1C) ZC2HC1 family Q53FD0
LIM domain-containing protein ajuba (AJUBA) Zyxin/ajuba family Q96IF1
Coiled-coil domain-containing protein 102B (CCDC102B) . Q68D86
Coiled-coil domain-containing protein 125 (CCDC125) . Q86Z20
G protein-regulated inducer of neurite outgrowth 2 (GPRIN2) . O60269
Melanoma-associated antigen 1 (MAGEA1) . P43355
RalBP1-associated Eps domain-containing protein 2 (REPS2) . Q8NFH8

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
5 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
6 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
7 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
8 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
9 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
10 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
11 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
12 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
13 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
14 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
15 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
16 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
17 Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning. Biochemistry. 2018 Jan 30;57(4):451-460. doi: 10.1021/acs.biochem.7b00897. Epub 2017 Oct 26.
18 Site-Specific Activity-Based Protein Profiling Using Phosphonate Handles. Mol Cell Proteomics. 2023 Jan;22(1):100455. doi: 10.1016/j.mcpro.2022.100455. Epub 2022 Nov 24.
Mass spectrometry data entry: PXD036569
19 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
20 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
21 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
22 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
23 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem Biol. 2019 Nov 15;14(11):2430-2440. doi: 10.1021/acschembio.8b01083. Epub 2019 May 13.
24 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
25 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
26 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
27 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
28 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.