Details of the Target

General Information of Target

Target ID LDTP12923
Target Name EH domain-containing protein 2 (EHD2)
Gene Name EHD2
Gene ID 30846
Synonyms
PAST2; EH domain-containing protein 2; PAST homolog 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MFSWLGTDDRRRKDPEVFQTVSEGLKKLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPM
VLLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMQGDMEGIIPGNALVVDPKK
PFRKLNAFGNAFLNRFVCAQLPNPVLESISVIDTPGILSGEKQRISRGYDFAAVLEWFAE
RVDRIILLFDAHKLDISDEFSEVIKALKNHEDKMRVVLNKADQIETQQLMRVYGALMWSL
GKIVNTPEVIRVYIGSFWSHPLLIPDNRKLFEAEEQDLFRDIQSLPRNAALRKLNDLIKR
ARLAKVHAYIISSLKKEMPSVFGKDNKKKELVNNLAEIYGRIEREHQISPGDFPNLKRMQ
DQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHG
PFGHGYGEGAGEGIDDAEWVVARDKPMYDEIFYTLSPVDGKITGANAKKEMVRSKLPNSV
LGKIWKLADIDKDGMLDDDEFALANHLIKVKLEGHELPNELPAHLLPPSKRKVAE
Target Bioclass
Enzyme
Family
TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, EHD subfamily
Subcellular location
Cell membrane
Function
ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane. Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane.
Uniprot ID
Q9NZN4
Ensemble ID
ENST00000263277.8
HGNC ID
HGNC:3243

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.H192Y .
CHL1 SNV: p.P395L .
HEC1 SNV: p.A365T .
HEC1B SNV: p.A365T .
HT115 SNV: p.D494N .
IGR1 SNV: p.S349F .
JURKAT SNV: p.S68N .
MEWO SNV: p.P482L .
SNU1079 SNV: p.R231C .
SW480 SNV: p.A308D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P2
 Probe Info  		4.08  LDD0449  [1]
ONAyne
 Probe Info  		K213(0.93); K32(0.75)  LDD0274  [2]
STPyne
 Probe Info  		K124(8.71); K213(10.00); K220(6.46); K299(6.67)  LDD0277  [2]
OPA-S-S-alkyne
 Probe Info  		K491(1.25); K220(2.14); K32(2.92)  LDD3494  [3]
BTD
 Probe Info  		C356(0.73)  LDD2107  [4]
5E-2FA
 Probe Info  		H46(0.00); H520(0.00)  LDD2235  [5]
m-APA
 Probe Info  		H46(0.00); H520(0.00)  LDD2231  [5]
IPM
 Probe Info  		N.A.  LDD0025  [6]
NAIA_4
 Probe Info  		C96(0.00); C138(0.00); C356(0.00)  LDD2226  [7]
WYneN
 Probe Info  		N.A.  LDD0021  [8]
Acrolein
 Probe Info  		C356(0.00); C96(0.00); H511(0.00)  LDD0217  [9]
NAIA_5
 Probe Info  		N.A.  LDD2223  [7]
HHS-465
 Probe Info  		Y67(0.00); K71(0.00)  LDD2240  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C356(1.53)  LDD2152  [4]
 LDCM0108  Chloroacetamide HeLa C356(0.00); H372(0.00); H388(0.00); H511(0.00)  LDD0222  [9]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C356(10.76)  LDD1702  [4]
 LDCM0107  IAA HeLa N.A.  LDD0221  [9]
 LDCM0023  KB03 MDA-MB-231 C356(1.70)  LDD1701  [4]
 LDCM0109  NEM HeLa H366(0.00); H520(0.00); H372(0.00); H511(0.00)  LDD0223  [9]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C356(0.73)  LDD2107  [4]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C356(1.08)  LDD2111  [4]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C356(0.99)  LDD2129  [4]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C356(0.82)  LDD2150  [4]

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
10 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010