Details of the Target

General Information of Target

Target ID LDTP05609
Target Name Ras-GEF domain-containing family member 1B (RASGEF1B)
Gene Name RASGEF1B
Gene ID 153020
Synonyms
GPIG4; Ras-GEF domain-containing family member 1B; GPI gamma-4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDR
TYIFTFLLSSRLFMHPYELMAKVCHLCVEHQRLSDPDSDKNQMRKIAPKILQLLTEWTET
FPYDFRDERMMRNLKDLAHRIASGEEQTYRKNVQQMMQCLIRKLAALSQYEEVLAKISST
STDRLTVLKTKPQSIQRDIITVCNDPYTLAQQLTHIELERLNYIGPEEFVQAFVQKDPLD
NDKSCYSERKKTRNLEAYVEWFNRLSYLVATEICMPVKKKHRARMIEYFIDVARECFNIG
NFNSLMAIISGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGAAQRSL
TAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVSEFMTWKQVEC
PFERDRKILQYLLTVPVFSEDALYLASYESEGPENHIEKDRWKSLRSSLLGRV
Target Bioclass
Other
Subcellular location
Early endosome
Function Guanine nucleotide exchange factor (GEF) with specificity for RAP2A, it doesn't seems to activate other Ras family proteins (in vitro).
Uniprot ID
Q0VAM2
Ensemble ID
ENST00000264400.7
HGNC ID
HGNC:24881

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DU145 Insertion: p.H281TfsTer7 .
HCT15 SNV: p.S359Y; p.S468N .
KYSE510 SNV: p.P6L .
MDAMB468 SNV: p.D97Y .
OCUM1 SNV: p.S310Y .
U118MG SNV: p.E128Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P2
 Probe Info  		1.64  LDD0453  [1]
ONAyne
 Probe Info  		K135(1.15)  LDD0274  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bromodomain adjacent to zinc finger domain protein 2B (BAZ2B) WAL family Q9UIF8
PDZ and LIM domain protein 7 (PDLIM7) . Q9NR12
Pleckstrin homology domain-containing family F member 2 (PLEKHF2) . Q9H8W4
Testis-specific protein 10-interacting protein (TSGA10IP) . Q3SY00

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270