Details of the Target

General Information of Target

Target ID LDTP06810
Target Name Mitochondrial import inner membrane translocase subunit TIM50 (TIMM50)
Gene Name TIMM50
Gene ID 92609
Synonyms
TIM50; Mitochondrial import inner membrane translocase subunit TIM50
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MAASAAVFSRLRSGLRLGSRGLCTRLATPPRRAPDQAAEIGSRGSTKAQGPQQQPGSEGP
SYAKKVALWLAGLLGAGGTVSVVYIFGNNPVDENGAKIPDEFDNDPILVQQLRRTYKYFK
DYRQMIIEPTSPCLLPDPLQEPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETL
FQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMDGHHVKDISCLNRD
PARVVVVDCKKEAFRLQPYNGVALRPWDGNSDDRVLLDLSAFLKTIALNGVEDVRTVLEH
YALEDDPLAAFKQRQSRLEQEEQQRLAELSKSNKQNLFLGSLTSRLWPRSKQP
Target Bioclass
Enzyme
Family
TIM50 family
Subcellular location
Mitochondrion inner membrane; Nucleus speckle
Function
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.; [Isoform 2]: May participate in the release of snRNPs and SMN from the Cajal body.
Uniprot ID
Q3ZCQ8
Ensemble ID
ENST00000544017.5
HGNC ID
HGNC:23656
ChEMBL ID
CHEMBL4295842

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A498 SNV: p.A6E .
CAL27 SNV: p.L339F .
LS180 SNV: p.V80L .
MFE319 SNV: p.Q124Ter .
NB1 SNV: p.Q53H DBIA    Probe Info 
NCIH358 SNV: p.D137H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 31 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
CHEMBL5175495
 Probe Info  		6.72  LDD0196  [2]
CY-1
 Probe Info  		100.00  LDD0243  [3]
TH211
 Probe Info  		Y225(20.00); Y62(10.50)  LDD0260  [4]
YN-1
 Probe Info  		100.00  LDD0444  [5]
ONAyne
 Probe Info  		N.A.  LDD0273  [6]
BTD
 Probe Info  		C236(1.28)  LDD1700  [7]
HPAP
 Probe Info  		4.65  LDD0062  [8]
DBIA
 Probe Info  		C249(7.78)  LDD0209  [9]
5E-2FA
 Probe Info  		N.A.  LDD2235  [10]
ATP probe
 Probe Info  		K64(0.00); K65(0.00)  LDD0199  [11]
4-Iodoacetamidophenylacetylene
 Probe Info  		C249(0.00); C236(0.00)  LDD0038  [12]
IA-alkyne
 Probe Info  		C236(0.00); C249(0.00)  LDD0032  [13]
Lodoacetamide azide
 Probe Info  		C249(0.00); C236(0.00)  LDD0037  [12]
NAIA_4
 Probe Info  		N.A.  LDD2226  [14]
WYneN
 Probe Info  		C236(0.00); C249(0.00)  LDD0021  [15]
WYneO
 Probe Info  		N.A.  LDD0022  [15]
IPM
 Probe Info  		N.A.  LDD0005  [15]
NHS
 Probe Info  		K334(0.00); K65(0.00); K64(0.00)  LDD0010  [15]
SF
 Probe Info  		N.A.  LDD0028  [16]
STPyne
 Probe Info  		K64(0.00); K334(0.00); K65(0.00)  LDD0009  [15]
TFBX
 Probe Info  		C236(0.00); C249(0.00)  LDD0148  [17]
VSF
 Probe Info  		N.A.  LDD0007  [15]
Phosphinate-6
 Probe Info  		C249(0.00); C236(0.00)  LDD0018  [18]
1c-yne
 Probe Info  		K64(0.00); K334(0.00)  LDD0228  [19]
Acrolein
 Probe Info  		H300(0.00); C249(0.00); H230(0.00)  LDD0217  [20]
Crotonaldehyde
 Probe Info  		H230(0.00); C236(0.00); C249(0.00); H229(0.00)  LDD0219  [20]
Methacrolein
 Probe Info  		N.A.  LDD0218  [20]
W1
 Probe Info  		C236(0.00); C249(0.00)  LDD0236  [21]
AOyne
 Probe Info  		15.00  LDD0443  [22]
NAIA_5
 Probe Info  		C236(0.00); C249(0.00)  LDD2223  [14]
PAL-AfBPP Probe
Click To Hide/Show 22 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C056
 Probe Info  		17.15  LDD1753  [23]
C169
 Probe Info  		22.63  LDD1849  [23]
C218
 Probe Info  		11.31  LDD1892  [23]
C228
 Probe Info  		15.89  LDD1901  [23]
C231
 Probe Info  		11.47  LDD1904  [23]
C235
 Probe Info  		19.29  LDD1908  [23]
C269
 Probe Info  		64.89  LDD1939  [23]
C278
 Probe Info  		55.72  LDD1948  [23]
C338
 Probe Info  		16.68  LDD2001  [23]
C355
 Probe Info  		27.86  LDD2016  [23]
C388
 Probe Info  		37.27  LDD2047  [23]
FFF probe11
 Probe Info  		19.13  LDD0471  [24]
FFF probe13
 Probe Info  		20.00  LDD0475  [24]
FFF probe14
 Probe Info  		20.00  LDD0477  [24]
FFF probe2
 Probe Info  		20.00  LDD0463  [24]
FFF probe3
 Probe Info  		16.23  LDD0464  [24]
FFF probe4
 Probe Info  		14.29  LDD0466  [24]
STS-2
 Probe Info  		N.A.  LDD0138  [25]
VE-P
 Probe Info  		N.A.  LDD0396  [26]
Alk-rapa
 Probe Info  		7.15  LDD0213  [27]
BD-F
 Probe Info  		P60(0.00); G59(0.00); S57(0.00)  LDD0024  [28]
OEA-DA
 Probe Info  		15.93  LDD0046  [29]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C236(0.75)  LDD2142  [7]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C236(0.86)  LDD2112  [7]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C249(0.68)  LDD2095  [7]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C249(1.22)  LDD2130  [7]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C236(0.84)  LDD2117  [7]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C236(1.20)  LDD2152  [7]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C236(0.56)  LDD2132  [7]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C249(0.73)  LDD2131  [7]
 LDCM0214  AC1 HEK-293T C236(0.97); C249(0.94)  LDD1507  [30]
 LDCM0215  AC10 HEK-293T C236(0.94); C249(0.93); C133(0.91)  LDD1508  [30]
 LDCM0226  AC11 HEK-293T C236(1.03); C249(0.97)  LDD1509  [30]
 LDCM0237  AC12 HEK-293T C236(0.92); C249(0.97)  LDD1510  [30]
 LDCM0259  AC14 HEK-293T C236(0.93); C249(0.89); C133(1.07)  LDD1512  [30]
 LDCM0270  AC15 HEK-293T C236(0.89); C249(0.90)  LDD1513  [30]
 LDCM0276  AC17 HEK-293T C236(1.06); C249(0.96)  LDD1515  [30]
 LDCM0277  AC18 HEK-293T C236(1.03); C249(1.05); C133(0.83)  LDD1516  [30]
 LDCM0278  AC19 HEK-293T C236(0.99); C249(1.15)  LDD1517  [30]
 LDCM0279  AC2 HEK-293T C236(0.94); C249(0.87); C133(0.99)  LDD1518  [30]
 LDCM0280  AC20 HEK-293T C236(1.00); C249(1.05)  LDD1519  [30]
 LDCM0281  AC21 HEK-293T C236(1.07); C249(1.05)  LDD1520  [30]
 LDCM0282  AC22 HEK-293T C236(1.02); C249(1.01); C133(1.62)  LDD1521  [30]
 LDCM0283  AC23 HEK-293T C236(0.90); C249(0.94)  LDD1522  [30]
 LDCM0284  AC24 HEK-293T C236(0.98); C249(0.95)  LDD1523  [30]
 LDCM0285  AC25 HEK-293T C236(1.04); C249(1.01)  LDD1524  [30]
 LDCM0286  AC26 HEK-293T C236(0.96); C249(1.13); C133(1.21)  LDD1525  [30]
 LDCM0287  AC27 HEK-293T C236(1.06); C249(1.13)  LDD1526  [30]
 LDCM0288  AC28 HEK-293T C236(1.02); C249(1.04)  LDD1527  [30]
 LDCM0289  AC29 HEK-293T C236(0.87); C249(1.05)  LDD1528  [30]
 LDCM0290  AC3 HEK-293T C236(0.96); C249(0.96)  LDD1529  [30]
 LDCM0291  AC30 HEK-293T C236(1.01); C249(1.02); C133(1.54)  LDD1530  [30]
 LDCM0292  AC31 HEK-293T C236(0.96); C249(1.02)  LDD1531  [30]
 LDCM0293  AC32 HEK-293T C236(1.00); C249(1.05)  LDD1532  [30]
 LDCM0294  AC33 HEK-293T C236(1.05); C249(0.89)  LDD1533  [30]
 LDCM0295  AC34 HEK-293T C236(0.98); C249(0.96); C133(1.04)  LDD1534  [30]
 LDCM0296  AC35 HEK-293T C236(1.01); C249(0.94)  LDD1535  [30]
 LDCM0297  AC36 HEK-293T C236(0.92); C249(1.00)  LDD1536  [30]
 LDCM0298  AC37 HEK-293T C236(0.93); C249(0.88)  LDD1537  [30]
 LDCM0299  AC38 HEK-293T C236(0.99); C249(0.94); C133(1.08)  LDD1538  [30]
 LDCM0300  AC39 HEK-293T C236(0.89); C249(0.94)  LDD1539  [30]
 LDCM0301  AC4 HEK-293T C236(1.03); C249(0.95)  LDD1540  [30]
 LDCM0302  AC40 HEK-293T C236(0.98); C249(0.91)  LDD1541  [30]
 LDCM0303  AC41 HEK-293T C236(0.96); C249(0.93)  LDD1542  [30]
 LDCM0304  AC42 HEK-293T C236(0.93); C249(0.95); C133(0.92)  LDD1543  [30]
 LDCM0305  AC43 HEK-293T C236(0.98); C249(0.92)  LDD1544  [30]
 LDCM0306  AC44 HEK-293T C236(0.97); C249(0.97)  LDD1545  [30]
 LDCM0307  AC45 HEK-293T C236(0.99); C249(0.95)  LDD1546  [30]
 LDCM0308  AC46 HEK-293T C236(0.95); C249(0.89); C133(1.74)  LDD1547  [30]
 LDCM0309  AC47 HEK-293T C236(0.86); C249(0.94)  LDD1548  [30]
 LDCM0310  AC48 HEK-293T C236(0.92); C249(0.83)  LDD1549  [30]
 LDCM0311  AC49 HEK-293T C236(1.05); C249(0.94)  LDD1550  [30]
 LDCM0312  AC5 HEK-293T C236(0.92); C249(0.95)  LDD1551  [30]
 LDCM0313  AC50 HEK-293T C236(0.94); C249(0.97); C133(1.27)  LDD1552  [30]
 LDCM0314  AC51 HEK-293T C236(1.02); C249(0.90)  LDD1553  [30]
 LDCM0315  AC52 HEK-293T C236(0.94); C249(1.01)  LDD1554  [30]
 LDCM0316  AC53 HEK-293T C236(1.01); C249(0.92)  LDD1555  [30]
 LDCM0317  AC54 HEK-293T C236(0.97); C249(0.92); C133(1.29)  LDD1556  [30]
 LDCM0318  AC55 HEK-293T C236(0.92); C249(0.92)  LDD1557  [30]
 LDCM0319  AC56 HEK-293T C236(0.98); C249(0.88)  LDD1558  [30]
 LDCM0320  AC57 HEK-293T C236(0.98); C249(0.95)  LDD1559  [30]
 LDCM0321  AC58 HEK-293T C236(0.99); C249(1.00); C133(1.07)  LDD1560  [30]
 LDCM0322  AC59 HEK-293T C236(1.03); C249(0.96)  LDD1561  [30]
 LDCM0323  AC6 HEK-293T C236(0.98); C249(0.98); C133(1.14)  LDD1562  [30]
 LDCM0324  AC60 HEK-293T C236(0.99); C249(0.95)  LDD1563  [30]
 LDCM0325  AC61 HEK-293T C236(1.18); C249(1.00)  LDD1564  [30]
 LDCM0326  AC62 HEK-293T C236(0.94); C249(0.98); C133(1.22)  LDD1565  [30]
 LDCM0327  AC63 HEK-293T C236(0.95); C249(0.99)  LDD1566  [30]
 LDCM0328  AC64 HEK-293T C236(0.99); C249(0.92)  LDD1567  [30]
 LDCM0334  AC7 HEK-293T C236(0.86); C249(0.92)  LDD1568  [30]
 LDCM0345  AC8 HEK-293T C236(0.95); C249(0.92)  LDD1569  [30]
 LDCM0545  Acetamide MDA-MB-231 C249(0.57)  LDD2138  [7]
 LDCM0520  AKOS000195272 MDA-MB-231 C236(0.60)  LDD2113  [7]
 LDCM0248  AKOS034007472 HEK-293T C236(0.88); C249(0.90)  LDD1511  [30]
 LDCM0356  AKOS034007680 HEK-293T C236(0.96); C249(0.92)  LDD1570  [30]
 LDCM0275  AKOS034007705 HEK-293T C236(0.95); C249(0.90)  LDD1514  [30]
 LDCM0156  Aniline NCI-H1299 12.03  LDD0403  [1]
 LDCM0498  BS-3668 MDA-MB-231 C249(0.40); C236(0.46)  LDD2091  [7]
 LDCM0108  Chloroacetamide HeLa H300(0.00); C249(0.00); H230(0.00)  LDD0222  [20]
 LDCM0367  CL1 HEK-293T C236(1.07); C249(0.87); C133(1.50)  LDD1571  [30]
 LDCM0368  CL10 HEK-293T C236(0.90); C249(0.50); C133(1.10)  LDD1572  [30]
 LDCM0369  CL100 HEK-293T C236(1.03); C249(0.96); C133(1.00)  LDD1573  [30]
 LDCM0370  CL101 HEK-293T C236(1.03); C249(0.90); C133(1.13)  LDD1574  [30]
 LDCM0371  CL102 HEK-293T C236(0.93); C249(0.91)  LDD1575  [30]
 LDCM0372  CL103 HEK-293T C236(0.85); C249(0.92)  LDD1576  [30]
 LDCM0373  CL104 HEK-293T C236(0.96); C249(0.96); C133(1.23)  LDD1577  [30]
 LDCM0374  CL105 HEK-293T C236(1.03); C249(0.98); C133(1.31)  LDD1578  [30]
 LDCM0375  CL106 HEK-293T C236(0.92); C249(0.97)  LDD1579  [30]
 LDCM0376  CL107 HEK-293T C236(0.94); C249(1.00)  LDD1580  [30]
 LDCM0377  CL108 HEK-293T C236(0.99); C249(1.05); C133(1.29)  LDD1581  [30]
 LDCM0378  CL109 HEK-293T C236(1.00); C249(1.01); C133(1.13)  LDD1582  [30]
 LDCM0379  CL11 HEK-293T C236(0.77); C249(0.33)  LDD1583  [30]
 LDCM0380  CL110 HEK-293T C236(0.93); C249(1.01)  LDD1584  [30]
 LDCM0381  CL111 HEK-293T C236(0.95); C249(1.01)  LDD1585  [30]
 LDCM0382  CL112 HEK-293T C236(1.00); C249(1.02); C133(0.98)  LDD1586  [30]
 LDCM0383  CL113 HEK-293T C236(1.04); C249(0.91); C133(1.32)  LDD1587  [30]
 LDCM0384  CL114 HEK-293T C236(1.00); C249(0.85)  LDD1588  [30]
 LDCM0385  CL115 HEK-293T C236(0.96); C249(0.90)  LDD1589  [30]
 LDCM0386  CL116 HEK-293T C236(0.99); C249(0.96); C133(1.10)  LDD1590  [30]
 LDCM0387  CL117 HEK-293T C236(1.12); C249(0.97); C133(1.30)  LDD1591  [30]
 LDCM0388  CL118 HEK-293T C236(1.00); C249(0.93)  LDD1592  [30]
 LDCM0389  CL119 HEK-293T C236(0.89); C249(0.96)  LDD1593  [30]
 LDCM0390  CL12 HEK-293T C236(1.03); C249(0.94)  LDD1594  [30]
 LDCM0391  CL120 HEK-293T C236(1.00); C249(1.01); C133(1.35)  LDD1595  [30]
 LDCM0392  CL121 HEK-293T C236(1.15); C249(0.94); C133(1.20)  LDD1596  [30]
 LDCM0393  CL122 HEK-293T C236(1.00); C249(0.92)  LDD1597  [30]
 LDCM0394  CL123 HEK-293T C236(0.93); C249(0.84)  LDD1598  [30]
 LDCM0395  CL124 HEK-293T C236(1.00); C249(0.94); C133(1.29)  LDD1599  [30]
 LDCM0396  CL125 HEK-293T C236(1.33); C249(0.93); C133(1.18)  LDD1600  [30]
 LDCM0397  CL126 HEK-293T C236(1.16); C249(0.98)  LDD1601  [30]
 LDCM0398  CL127 HEK-293T C236(1.12); C249(0.94)  LDD1602  [30]
 LDCM0399  CL128 HEK-293T C236(1.07); C249(1.04); C133(1.45)  LDD1603  [30]
 LDCM0400  CL13 HEK-293T C236(1.13); C249(0.86); C133(1.45)  LDD1604  [30]
 LDCM0401  CL14 HEK-293T C236(0.97); C249(0.93)  LDD1605  [30]
 LDCM0402  CL15 HEK-293T C236(0.74); C249(0.80)  LDD1606  [30]
 LDCM0403  CL16 HEK-293T C236(0.87); C249(1.00); C133(1.21)  LDD1607  [30]
 LDCM0404  CL17 HEK-293T C236(0.84); C249(0.89)  LDD1608  [30]
 LDCM0405  CL18 HEK-293T C236(0.90); C249(0.96); C133(1.15)  LDD1609  [30]
 LDCM0406  CL19 HEK-293T C236(1.03); C249(0.91)  LDD1610  [30]
 LDCM0407  CL2 HEK-293T C236(0.95); C249(0.89)  LDD1611  [30]
 LDCM0408  CL20 HEK-293T C236(0.66); C249(1.06)  LDD1612  [30]
 LDCM0409  CL21 HEK-293T C236(0.59); C249(0.73)  LDD1613  [30]
 LDCM0410  CL22 HEK-293T C236(1.07); C249(0.46); C133(0.79)  LDD1614  [30]
 LDCM0411  CL23 HEK-293T C236(0.79); C249(0.29)  LDD1615  [30]
 LDCM0412  CL24 HEK-293T C236(1.01); C249(0.99)  LDD1616  [30]
 LDCM0413  CL25 HEK-293T C236(1.07); C249(0.95); C133(1.56)  LDD1617  [30]
 LDCM0414  CL26 HEK-293T C236(1.01); C249(0.99)  LDD1618  [30]
 LDCM0415  CL27 HEK-293T C236(0.93); C249(0.91)  LDD1619  [30]
 LDCM0416  CL28 HEK-293T C236(0.94); C249(0.96); C133(1.67)  LDD1620  [30]
 LDCM0417  CL29 HEK-293T C236(0.96); C249(0.95)  LDD1621  [30]
 LDCM0418  CL3 HEK-293T C236(0.88); C249(0.85)  LDD1622  [30]
 LDCM0419  CL30 HEK-293T C236(0.93); C249(1.01); C133(1.34)  LDD1623  [30]
 LDCM0420  CL31 HEK-293T C236(1.01); C249(1.05)  LDD1624  [30]
 LDCM0421  CL32 HEK-293T C236(0.75); C249(1.04)  LDD1625  [30]
 LDCM0422  CL33 HEK-293T C236(0.74); C249(0.79)  LDD1626  [30]
 LDCM0423  CL34 HEK-293T C236(1.21); C249(0.48); C133(0.66)  LDD1627  [30]
 LDCM0424  CL35 HEK-293T C236(0.77); C249(0.28)  LDD1628  [30]
 LDCM0425  CL36 HEK-293T C236(1.04); C249(1.05)  LDD1629  [30]
 LDCM0426  CL37 HEK-293T C236(1.09); C249(0.97); C133(1.79)  LDD1630  [30]
 LDCM0428  CL39 HEK-293T C236(0.88); C249(0.95)  LDD1632  [30]
 LDCM0429  CL4 HEK-293T C236(0.91); C249(0.94); C133(1.41)  LDD1633  [30]
 LDCM0430  CL40 HEK-293T C236(0.95); C249(0.99); C133(1.74)  LDD1634  [30]
 LDCM0431  CL41 HEK-293T C236(1.06); C249(0.93)  LDD1635  [30]
 LDCM0432  CL42 HEK-293T C236(0.99); C249(1.02); C133(1.62)  LDD1636  [30]
 LDCM0433  CL43 HEK-293T C236(1.06); C249(1.06)  LDD1637  [30]
 LDCM0434  CL44 HEK-293T C236(0.80); C249(1.08)  LDD1638  [30]
 LDCM0435  CL45 HEK-293T C236(0.83); C249(0.78)  LDD1639  [30]
 LDCM0436  CL46 HEK-293T C236(1.15); C249(0.49); C133(0.71)  LDD1640  [30]
 LDCM0437  CL47 HEK-293T C236(0.83); C249(0.31)  LDD1641  [30]
 LDCM0438  CL48 HEK-293T C236(0.98); C249(1.06)  LDD1642  [30]
 LDCM0439  CL49 HEK-293T C236(1.16); C249(0.93); C133(1.86)  LDD1643  [30]
 LDCM0440  CL5 HEK-293T C236(0.94); C249(0.83)  LDD1644  [30]
 LDCM0441  CL50 HEK-293T C236(0.96); C249(0.91)  LDD1645  [30]
 LDCM0443  CL52 HEK-293T C236(0.91); C249(0.94); C133(2.61)  LDD1646  [30]
 LDCM0444  CL53 HEK-293T C236(0.85); C249(0.84)  LDD1647  [30]
 LDCM0445  CL54 HEK-293T C236(0.83); C249(0.85); C133(2.17)  LDD1648  [30]
 LDCM0446  CL55 HEK-293T C236(1.03); C249(0.98)  LDD1649  [30]
 LDCM0447  CL56 HEK-293T C236(0.74); C249(1.06)  LDD1650  [30]
 LDCM0448  CL57 HEK-293T C236(0.70); C249(0.80)  LDD1651  [30]
 LDCM0449  CL58 HEK-293T C236(1.04); C249(0.50); C133(1.10)  LDD1652  [30]
 LDCM0450  CL59 HEK-293T C236(0.79); C249(0.36)  LDD1653  [30]
 LDCM0451  CL6 HEK-293T C236(0.93); C249(0.83); C133(0.87)  LDD1654  [30]
 LDCM0452  CL60 HEK-293T C236(0.95); C249(0.94)  LDD1655  [30]
 LDCM0453  CL61 HEK-293T C236(1.19); C249(0.92); C133(1.29)  LDD1656  [30]
 LDCM0454  CL62 HEK-293T C236(1.06); C249(0.89)  LDD1657  [30]
 LDCM0455  CL63 HEK-293T C236(0.91); C249(0.94)  LDD1658  [30]
 LDCM0456  CL64 HEK-293T C236(0.85); C249(0.89); C133(1.50)  LDD1659  [30]
 LDCM0457  CL65 HEK-293T C236(0.96); C249(0.95)  LDD1660  [30]
 LDCM0458  CL66 HEK-293T C236(0.92); C249(0.93); C133(1.29)  LDD1661  [30]
 LDCM0459  CL67 HEK-293T C236(1.01); C249(0.94)  LDD1662  [30]
 LDCM0460  CL68 HEK-293T C236(0.78); C249(1.02)  LDD1663  [30]
 LDCM0461  CL69 HEK-293T C236(0.81); C249(0.83)  LDD1664  [30]
 LDCM0462  CL7 HEK-293T C236(0.94); C249(0.88)  LDD1665  [30]
 LDCM0463  CL70 HEK-293T C236(1.10); C249(0.57); C133(0.65)  LDD1666  [30]
 LDCM0464  CL71 HEK-293T C236(0.80); C249(0.38)  LDD1667  [30]
 LDCM0465  CL72 HEK-293T C236(0.93); C249(0.99)  LDD1668  [30]
 LDCM0466  CL73 HEK-293T C236(1.10); C249(0.88); C133(1.63)  LDD1669  [30]
 LDCM0467  CL74 HEK-293T C236(1.09); C249(0.91)  LDD1670  [30]
 LDCM0469  CL76 HEK-293T C236(0.99); C249(0.96); C133(2.03)  LDD1672  [30]
 LDCM0470  CL77 HEK-293T C236(0.93); C249(0.85)  LDD1673  [30]
 LDCM0471  CL78 HEK-293T C236(0.93); C249(0.98); C133(1.25)  LDD1674  [30]
 LDCM0472  CL79 HEK-293T C236(1.02); C249(0.97)  LDD1675  [30]
 LDCM0473  CL8 HEK-293T C236(0.57); C249(0.82)  LDD1676  [30]
 LDCM0474  CL80 HEK-293T C236(0.82); C249(1.02)  LDD1677  [30]
 LDCM0475  CL81 HEK-293T C236(0.81); C249(0.86)  LDD1678  [30]
 LDCM0476  CL82 HEK-293T C236(1.10); C249(0.67); C133(0.96)  LDD1679  [30]
 LDCM0477  CL83 HEK-293T C236(0.87); C249(0.51)  LDD1680  [30]
 LDCM0478  CL84 HEK-293T C236(0.95); C249(0.91)  LDD1681  [30]
 LDCM0479  CL85 HEK-293T C236(1.28); C249(0.89); C133(1.73)  LDD1682  [30]
 LDCM0480  CL86 HEK-293T C236(1.49); C249(0.95)  LDD1683  [30]
 LDCM0481  CL87 HEK-293T C236(1.22); C249(0.91)  LDD1684  [30]
 LDCM0482  CL88 HEK-293T C236(1.03); C249(0.94); C133(1.35)  LDD1685  [30]
 LDCM0483  CL89 HEK-293T C236(1.02); C249(0.89)  LDD1686  [30]
 LDCM0484  CL9 HEK-293T C236(0.78); C249(0.78)  LDD1687  [30]
 LDCM0485  CL90 HEK-293T C236(0.77); C249(0.85); C133(1.06)  LDD1688  [30]
 LDCM0486  CL91 HEK-293T C236(0.98); C249(0.97)  LDD1689  [30]
 LDCM0487  CL92 HEK-293T C236(0.90); C249(0.90)  LDD1690  [30]
 LDCM0488  CL93 HEK-293T C236(0.73); C249(0.84)  LDD1691  [30]
 LDCM0489  CL94 HEK-293T C236(1.02); C249(0.75); C133(0.81)  LDD1692  [30]
 LDCM0490  CL95 HEK-293T C236(0.72); C249(0.65)  LDD1693  [30]
 LDCM0491  CL96 HEK-293T C236(0.93); C249(0.83)  LDD1694  [30]
 LDCM0492  CL97 HEK-293T C236(1.05); C249(0.91); C133(1.27)  LDD1695  [30]
 LDCM0493  CL98 HEK-293T C236(1.02); C249(0.88)  LDD1696  [30]
 LDCM0494  CL99 HEK-293T C236(1.03); C249(0.84)  LDD1697  [30]
 LDCM0495  E2913 HEK-293T C236(0.94); C249(0.92)  LDD1698  [30]
 LDCM0625  F8 Ramos C249(1.13); C236(1.37)  LDD2187  [31]
 LDCM0572  Fragment10 Ramos C249(1.34); C236(1.21)  LDD2189  [31]
 LDCM0573  Fragment11 Ramos C249(0.05); C236(0.52)  LDD2190  [31]
 LDCM0574  Fragment12 Ramos C249(1.06); C236(1.34)  LDD2191  [31]
 LDCM0575  Fragment13 Ramos C249(1.05); C236(1.09)  LDD2192  [31]
 LDCM0576  Fragment14 Ramos C249(1.39); C236(1.18)  LDD2193  [31]
 LDCM0579  Fragment20 Ramos C249(1.02); C236(1.34)  LDD2194  [31]
 LDCM0580  Fragment21 Ramos C249(0.87); C236(1.09)  LDD2195  [31]
 LDCM0582  Fragment23 Ramos C249(0.74); C236(0.57)  LDD2196  [31]
 LDCM0578  Fragment27 Ramos C249(0.94); C236(1.15)  LDD2197  [31]
 LDCM0586  Fragment28 Ramos C249(0.87); C236(0.81)  LDD2198  [31]
 LDCM0588  Fragment30 Ramos C249(1.06); C236(1.20)  LDD2199  [31]
 LDCM0589  Fragment31 Ramos C249(1.02); C236(0.99)  LDD2200  [31]
 LDCM0590  Fragment32 Ramos C249(0.89); C236(1.35)  LDD2201  [31]
 LDCM0468  Fragment33 HEK-293T C236(0.99); C249(0.91)  LDD1671  [30]
 LDCM0596  Fragment38 Ramos C249(1.11); C236(0.81)  LDD2203  [31]
 LDCM0566  Fragment4 Ramos C249(1.10); C236(1.38)  LDD2184  [31]
 LDCM0427  Fragment51 HEK-293T C236(0.98); C249(1.01)  LDD1631  [30]
 LDCM0610  Fragment52 Ramos C249(1.34); C236(1.51)  LDD2204  [31]
 LDCM0614  Fragment56 Ramos C249(1.52); C236(1.34)  LDD2205  [31]
 LDCM0569  Fragment7 Ramos C249(0.99); C236(1.42)  LDD2186  [31]
 LDCM0571  Fragment9 Ramos C249(1.24); C236(2.84)  LDD2188  [31]
 LDCM0107  IAA HeLa H300(0.00); C249(0.00)  LDD0221  [20]
 LDCM0022  KB02 HEK-293T C236(0.96); C249(0.93)  LDD1492  [30]
 LDCM0023  KB03 Jurkat C249(7.78)  LDD0209  [9]
 LDCM0024  KB05 COLO792 C352(2.45)  LDD3310  [32]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C236(0.70)  LDD2102  [7]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C249(0.61); C236(1.00)  LDD2121  [7]
 LDCM0109  NEM HeLa H300(0.00); H229(0.00)  LDD0223  [20]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C236(1.30)  LDD2090  [7]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C236(0.81)  LDD2092  [7]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C236(0.80)  LDD2093  [7]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C236(0.80)  LDD2098  [7]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C236(0.83)  LDD2099  [7]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C236(0.85)  LDD2100  [7]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C236(0.62)  LDD2104  [7]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C236(0.85)  LDD2107  [7]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C236(0.74)  LDD2109  [7]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C236(0.86)  LDD2110  [7]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C236(0.95)  LDD2111  [7]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C236(1.97)  LDD2119  [7]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C236(0.86)  LDD2123  [7]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C236(0.66)  LDD2125  [7]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C236(0.43)  LDD2126  [7]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C236(0.83)  LDD2127  [7]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C236(0.58)  LDD2128  [7]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C236(0.95)  LDD2129  [7]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C236(1.23)  LDD2135  [7]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C236(0.93)  LDD2137  [7]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C236(1.28)  LDD1700  [7]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C236(0.63)  LDD2140  [7]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C236(0.65)  LDD2141  [7]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C236(2.72)  LDD2144  [7]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C236(0.72)  LDD2153  [7]
 LDCM0627  NUDT7-COV-1 HEK-293T C249(1.06); C236(0.80)  LDD2206  [33]
 LDCM0628  OTUB2-COV-1 HEK-293T C249(0.57); C236(0.56)  LDD2207  [33]
 LDCM0014  Panhematin HEK-293T 4.65  LDD0062  [8]
 LDCM0090  Rapamycin JHH-7 7.15  LDD0213  [27]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
RAF proto-oncogene serine/threonine-protein kinase (RAF1) TKL Ser/Thr protein kinase family P04049
Serine/threonine-protein kinase A-Raf (ARAF) TKL Ser/Thr protein kinase family P10398

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Charting the Chemical Space of Acrylamide-Based Inhibitors of zDHHC20. ACS Med Chem Lett. 2022 Sep 26;13(10):1648-1654. doi: 10.1021/acsmedchemlett.2c00336. eCollection 2022 Oct 13.
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
6 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
7 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
8 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
9 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
10 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
11 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
12 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
13 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
14 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
15 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
16 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
17 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
18 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
19 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
20 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
21 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
22 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
23 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
24 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
25 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
26 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
27 Rapamycin targets STAT3 and impacts c-Myc to suppress tumor growth. Cell Chem Biol. 2022 Mar 17;29(3):373-385.e6. doi: 10.1016/j.chembiol.2021.10.006. Epub 2021 Oct 26.
28 Evaluation of fully-functionalized diazirine tags for chemical proteomic applications. Chem Sci. 2021 May 7;12(22):7839-7847. doi: 10.1039/d1sc01360b.
Mass spectrometry data entry: PXD025652
29 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
30 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
31 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
32 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
33 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.