Details of the Target

General Information of Target

Target ID LDTP01763
Target Name Cytochrome b5 (CYB5A)
Gene Name CYB5A
Gene ID 1528
Synonyms
CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDAT
ENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSWWTNWVIPAISA
VAVALMYRLYMAED
Target Bioclass
Enzyme
Family
Cytochrome b5 family
Subcellular location
Cytoplasm; Cytoplasmic side; Endoplasmic reticulum membrane; Single-pass membrane protein
Function Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.
Uniprot ID
P00167
Ensemble ID
ENST00000340533.9
HGNC ID
HGNC:2570
ChEMBL ID
CHEMBL6170

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
MKN45 SNV: p.A72T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K19(10.00)  LDD0277  [1]
HPAP
 Probe Info  		3.15  LDD0062  [2]
Acrolein
 Probe Info  		N.A.  LDD0224  [3]
5E-2FA
 Probe Info  		N.A.  LDD2235  [4]
m-APA
 Probe Info  		N.A.  LDD2231  [4]
PAL-AfBPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FFF probe11
 Probe Info  		20.00  LDD0471  [5]
FFF probe13
 Probe Info  		20.00  LDD0475  [5]
FFF probe14
 Probe Info  		20.00  LDD0477  [5]
FFF probe3
 Probe Info  		20.00  LDD0464  [5]
A-DA
 Probe Info  		2.17  LDD0141  [6]
OEA-DA
 Probe Info  		20.00  LDD0046  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0109  NEM HeLa N.A.  LDD0224  [3]
 LDCM0014  Panhematin HEK-293T 3.15  LDD0062  [2]
 LDCM0081  Rofecoxib A-549 2.17  LDD0141  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Wolframin (WFS1) . O76024
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Progranulin (GRN) Granulin family P28799
RING finger protein 11 (RNF11) . Q9Y3C5

The Drug(s) Related To This Target

Approved
Click To Hide/Show 5 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Chromic Nitrate . DB14527
Chromium . DB11136
Chromium Gluconate . DB14528
Chromium Nicotinate . DB14529
Chromous Sulfate . DB14530
Investigative
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Chromic Citrate . DB14526
Dimethyl Propionate Ester Heme . DB04009

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
6 A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells. Cell. 2015 Jun 18;161(7):1668-80. doi: 10.1016/j.cell.2015.05.045.
7 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570