Details of the Target

General Information of Target

Target ID LDTP14453
Target Name RNA-binding protein Musashi homolog 1 (MSI1)
Gene Name MSI1
Gene ID 4440
Synonyms
RNA-binding protein Musashi homolog 1; Musashi-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGSLTFRDVAIEFSLEEWQCLDTAQQNLYRNVMLENYRNLVFLGIAAFKPDLIIFLEEGK
ESWNMKRHEMVEESPVICSHFAQDLWPEQGIEDSFQKVILRRYEKCGHENLHLKIGYTNV
DECKVHKEGYNKLNQSLTTTQSKVFQRGKYANVFHKCSNSNRHKIRHTGKKHLQCKEYVR
SFCMLSHLSQHKRIYTRENSYKCEEGGKAFNWSSTLTYYKSAHTGEKPYRCKECGKAFSK
FSILTKHKVIHTGEKSYKCEECGKAFNQSAILTKHKIIHTGEKPNKCEECGKAFSKVSTL
TTHKAIHAGEKPYKCKECGKAFSKVSTLITHKAIHAGEKPYKCKECGKAFSKFSILTKHK
VIHTGEKPYKCEECGKAYKWPSTLSYHKKIHTGEKPYKCEECGKGFSMFSILTKHEVIHT
GEKPYKCEECGKAFNWSSNLMEHKKIHTGETPYKCEECGKGFSMFSILTKHKVIHNGEKP
YKCEECDKATHAGEKPYKCEECGKAFNWSSNLMEHKRIHTGEKPYKCEECGKSFSTFSIL
TKHKVIHTGEKPYKCEECGKAYKWSSTLSYHKKIHTVEKPYKCEECGKAFNQSAILIKHK
RIHTGEKPYKCEECGKTFSKVSTLTTHKAIHAGEKPYKCKECGKTFIKVSTLTTHKAIHA
GEKPYKCKECGKAFSKFSILTKHKVIHTGEKPYKCEECGKAFNWSSNLMEHKRIHTGEKP
YKCEECGKSFSTFSVLTKHKVIHTGEKPYKCEECGKAYKWSSTLSYHKKIHTVEKPYKCE
ECGKAFNRSAILIKHKRIHTDEKPYKCEECGKTFSKVSTLTTHKAIHAGEKPYKCKECGK
AFSKFSILTKHKVIHTGEKPYKCEECGKAYKWPSTLSYHKKIHTGEKPYKCEECGKGFSM
FSILTKHEVIHTGEKPYKCEECGKAFSWLSVFSKHKKTHAGEKFYKCEACGKAYKSSSTL
SYHKKIHTEEKPYKYEECGKGFSTFSILTKHKVIHTGEKPYKCEECGKAFNWSSNLMEHK
KIHTGETPYKCEECDKAFSWPSSLTEHKATHAGEKPYKCEECGKAFSWPSRLTEHKATHA
GEEPYKCEECGKAFNWSSNLMEHKRIHTGEKPYKCEECGKSFSTFSILTKHKVIHTGEKP
YKCEECGKAYKWSSTLSYHKKIHTVEKPYKCEECGKGFVMFSILAKHKVIHTGEKLYKCE
ECGKAYKWPSTLRYHKKIHTGEKPYKCEECGKAFSTFSILTKHKVIHTGEKPYKCEECGK
AFSWLSVFSKHKKIHTGEKL
Target Type
Literature-reported
Target Bioclass
Other
Family
Musashi family
Subcellular location
Cytoplasm
Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
TTD ID
T21715
Uniprot ID
O43347
DrugMap ID
TTSM4BA
Ensemble ID
ENST00000257552.7
HGNC ID
HGNC:7330

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y239(17.65); Y232(8.14); Y241(5.33)  LDD0257  [1]
TH216
 Probe Info  		Y241(12.43)  LDD0259  [1]
BTD
 Probe Info  		C167(2.55)  LDD1699  [2]
Probe 1
 Probe Info  		Y128(29.75)  LDD3495  [3]
HPAP
 Probe Info  		3.52  LDD0062  [4]
HHS-465
 Probe Info  		Y239(6.76)  LDD2237  [5]
Acrolein
 Probe Info  		N.A.  LDD0221  [6]
DBIA
 Probe Info  		C167(0.93)  LDD1492  [7]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [8]
IA-alkyne
 Probe Info  		N.A.  LDD0163  [9]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [8]
Methacrolein
 Probe Info  		N.A.  LDD0218  [6]
NAIA_5
 Probe Info  		N.A.  LDD2223  [10]
PAL-AfBPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C407
 Probe Info  		10.85  LDD2064  [11]
C409
 Probe Info  		5.13  LDD2066  [11]
FFF probe11
 Probe Info  		20.00  LDD0471  [12]
FFF probe13
 Probe Info  		20.00  LDD0475  [12]
FFF probe14
 Probe Info  		20.00  LDD0477  [12]
FFF probe2
 Probe Info  		20.00  LDD0463  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C167(0.92)  LDD1508  [7]
 LDCM0226  AC11 HEK-293T C167(1.03)  LDD1509  [7]
 LDCM0237  AC12 HEK-293T C167(1.08)  LDD1510  [7]
 LDCM0259  AC14 HEK-293T C167(1.02)  LDD1512  [7]
 LDCM0270  AC15 HEK-293T C167(0.99)  LDD1513  [7]
 LDCM0277  AC18 HEK-293T C167(0.88)  LDD1516  [7]
 LDCM0278  AC19 HEK-293T C167(0.80)  LDD1517  [7]
 LDCM0279  AC2 HEK-293T C167(0.97)  LDD1518  [7]
 LDCM0280  AC20 HEK-293T C167(1.08)  LDD1519  [7]
 LDCM0281  AC21 HEK-293T C167(1.01)  LDD1520  [7]
 LDCM0282  AC22 HEK-293T C167(1.00)  LDD1521  [7]
 LDCM0283  AC23 HEK-293T C167(1.05)  LDD1522  [7]
 LDCM0284  AC24 HEK-293T C167(1.01)  LDD1523  [7]
 LDCM0286  AC26 HEK-293T C167(1.00)  LDD1525  [7]
 LDCM0287  AC27 HEK-293T C167(1.02)  LDD1526  [7]
 LDCM0288  AC28 HEK-293T C167(1.07)  LDD1527  [7]
 LDCM0289  AC29 HEK-293T C167(1.02)  LDD1528  [7]
 LDCM0290  AC3 HEK-293T C167(1.12)  LDD1529  [7]
 LDCM0291  AC30 HEK-293T C167(1.08)  LDD1530  [7]
 LDCM0292  AC31 HEK-293T C167(1.03)  LDD1531  [7]
 LDCM0293  AC32 HEK-293T C167(0.92)  LDD1532  [7]
 LDCM0295  AC34 HEK-293T C167(1.00)  LDD1534  [7]
 LDCM0296  AC35 HEK-293T C167(1.03)  LDD1535  [7]
 LDCM0297  AC36 HEK-293T C167(1.07)  LDD1536  [7]
 LDCM0298  AC37 HEK-293T C167(1.20)  LDD1537  [7]
 LDCM0299  AC38 HEK-293T C167(1.01)  LDD1538  [7]
 LDCM0300  AC39 HEK-293T C167(1.00)  LDD1539  [7]
 LDCM0301  AC4 HEK-293T C167(1.14)  LDD1540  [7]
 LDCM0302  AC40 HEK-293T C167(0.98)  LDD1541  [7]
 LDCM0304  AC42 HEK-293T C167(0.94)  LDD1543  [7]
 LDCM0305  AC43 HEK-293T C167(1.17)  LDD1544  [7]
 LDCM0306  AC44 HEK-293T C167(1.17)  LDD1545  [7]
 LDCM0307  AC45 HEK-293T C167(1.08)  LDD1546  [7]
 LDCM0308  AC46 HEK-293T C167(1.07)  LDD1547  [7]
 LDCM0309  AC47 HEK-293T C167(1.03)  LDD1548  [7]
 LDCM0310  AC48 HEK-293T C167(1.02)  LDD1549  [7]
 LDCM0312  AC5 HEK-293T C167(1.14)  LDD1551  [7]
 LDCM0313  AC50 HEK-293T C167(0.95)  LDD1552  [7]
 LDCM0314  AC51 HEK-293T C167(1.05)  LDD1553  [7]
 LDCM0315  AC52 HEK-293T C167(0.99)  LDD1554  [7]
 LDCM0316  AC53 HEK-293T C167(1.08)  LDD1555  [7]
 LDCM0317  AC54 HEK-293T C167(0.97)  LDD1556  [7]
 LDCM0318  AC55 HEK-293T C167(0.97)  LDD1557  [7]
 LDCM0319  AC56 HEK-293T C167(1.00)  LDD1558  [7]
 LDCM0321  AC58 HEK-293T C167(0.93)  LDD1560  [7]
 LDCM0322  AC59 HEK-293T C167(1.17)  LDD1561  [7]
 LDCM0323  AC6 HEK-293T C167(1.02)  LDD1562  [7]
 LDCM0324  AC60 HEK-293T C167(0.99)  LDD1563  [7]
 LDCM0325  AC61 HEK-293T C167(1.06)  LDD1564  [7]
 LDCM0326  AC62 HEK-293T C167(0.99)  LDD1565  [7]
 LDCM0327  AC63 HEK-293T C167(0.94)  LDD1566  [7]
 LDCM0328  AC64 HEK-293T C167(1.01)  LDD1567  [7]
 LDCM0334  AC7 HEK-293T C167(1.05)  LDD1568  [7]
 LDCM0345  AC8 HEK-293T C167(0.96)  LDD1569  [7]
 LDCM0248  AKOS034007472 HEK-293T C167(1.06)  LDD1511  [7]
 LDCM0275  AKOS034007705 HEK-293T C167(1.00)  LDD1514  [7]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [6]
 LDCM0367  CL1 HEK-293T C167(0.96)  LDD1571  [7]
 LDCM0368  CL10 HEK-293T C167(0.88)  LDD1572  [7]
 LDCM0369  CL100 HEK-293T C167(0.93)  LDD1573  [7]
 LDCM0370  CL101 HEK-293T C167(0.98)  LDD1574  [7]
 LDCM0372  CL103 HEK-293T C167(0.96)  LDD1576  [7]
 LDCM0373  CL104 HEK-293T C167(1.02)  LDD1577  [7]
 LDCM0374  CL105 HEK-293T C167(1.00)  LDD1578  [7]
 LDCM0376  CL107 HEK-293T C167(0.97)  LDD1580  [7]
 LDCM0377  CL108 HEK-293T C167(1.00)  LDD1581  [7]
 LDCM0378  CL109 HEK-293T C167(0.99)  LDD1582  [7]
 LDCM0379  CL11 HEK-293T C167(1.01)  LDD1583  [7]
 LDCM0381  CL111 HEK-293T C167(0.97)  LDD1585  [7]
 LDCM0382  CL112 HEK-293T C167(0.94)  LDD1586  [7]
 LDCM0383  CL113 HEK-293T C167(1.04)  LDD1587  [7]
 LDCM0385  CL115 HEK-293T C167(0.97)  LDD1589  [7]
 LDCM0386  CL116 HEK-293T C167(0.98)  LDD1590  [7]
 LDCM0387  CL117 HEK-293T C167(1.05)  LDD1591  [7]
 LDCM0389  CL119 HEK-293T C167(1.10)  LDD1593  [7]
 LDCM0390  CL12 HEK-293T C167(0.95)  LDD1594  [7]
 LDCM0391  CL120 HEK-293T C167(1.08)  LDD1595  [7]
 LDCM0392  CL121 HEK-293T C167(0.94)  LDD1596  [7]
 LDCM0394  CL123 HEK-293T C167(0.87)  LDD1598  [7]
 LDCM0395  CL124 HEK-293T C167(0.95)  LDD1599  [7]
 LDCM0396  CL125 HEK-293T C167(1.00)  LDD1600  [7]
 LDCM0398  CL127 HEK-293T C167(0.94)  LDD1602  [7]
 LDCM0399  CL128 HEK-293T C167(0.99)  LDD1603  [7]
 LDCM0400  CL13 HEK-293T C167(0.95)  LDD1604  [7]
 LDCM0402  CL15 HEK-293T C167(0.82)  LDD1606  [7]
 LDCM0403  CL16 HEK-293T C167(0.95)  LDD1607  [7]
 LDCM0405  CL18 HEK-293T C167(0.88)  LDD1609  [7]
 LDCM0406  CL19 HEK-293T C167(1.17)  LDD1610  [7]
 LDCM0408  CL20 HEK-293T C167(1.10)  LDD1612  [7]
 LDCM0409  CL21 HEK-293T C167(0.96)  LDD1613  [7]
 LDCM0410  CL22 HEK-293T C167(1.12)  LDD1614  [7]
 LDCM0411  CL23 HEK-293T C167(1.00)  LDD1615  [7]
 LDCM0412  CL24 HEK-293T C167(1.08)  LDD1616  [7]
 LDCM0413  CL25 HEK-293T C167(1.05)  LDD1617  [7]
 LDCM0415  CL27 HEK-293T C167(1.03)  LDD1619  [7]
 LDCM0416  CL28 HEK-293T C167(0.94)  LDD1620  [7]
 LDCM0418  CL3 HEK-293T C167(1.00)  LDD1622  [7]
 LDCM0419  CL30 HEK-293T C167(1.02)  LDD1623  [7]
 LDCM0420  CL31 HEK-293T C167(1.01)  LDD1624  [7]
 LDCM0421  CL32 HEK-293T C167(1.18)  LDD1625  [7]
 LDCM0422  CL33 HEK-293T C167(0.98)  LDD1626  [7]
 LDCM0423  CL34 HEK-293T C167(1.04)  LDD1627  [7]
 LDCM0424  CL35 HEK-293T C167(1.05)  LDD1628  [7]
 LDCM0425  CL36 HEK-293T C167(1.08)  LDD1629  [7]
 LDCM0426  CL37 HEK-293T C167(1.11)  LDD1630  [7]
 LDCM0428  CL39 HEK-293T C167(0.95)  LDD1632  [7]
 LDCM0429  CL4 HEK-293T C167(0.99)  LDD1633  [7]
 LDCM0430  CL40 HEK-293T C167(0.98)  LDD1634  [7]
 LDCM0432  CL42 HEK-293T C167(1.01)  LDD1636  [7]
 LDCM0433  CL43 HEK-293T C167(1.18)  LDD1637  [7]
 LDCM0434  CL44 HEK-293T C167(1.11)  LDD1638  [7]
 LDCM0435  CL45 HEK-293T C167(0.97)  LDD1639  [7]
 LDCM0436  CL46 HEK-293T C167(1.05)  LDD1640  [7]
 LDCM0437  CL47 HEK-293T C167(1.02)  LDD1641  [7]
 LDCM0438  CL48 HEK-293T C167(0.98)  LDD1642  [7]
 LDCM0439  CL49 HEK-293T C167(1.15)  LDD1643  [7]
 LDCM0443  CL52 HEK-293T C167(1.04)  LDD1646  [7]
 LDCM0445  CL54 HEK-293T C167(0.83)  LDD1648  [7]
 LDCM0446  CL55 HEK-293T C167(1.15)  LDD1649  [7]
 LDCM0447  CL56 HEK-293T C167(1.17)  LDD1650  [7]
 LDCM0448  CL57 HEK-293T C167(1.03)  LDD1651  [7]
 LDCM0449  CL58 HEK-293T C167(1.22)  LDD1652  [7]
 LDCM0450  CL59 HEK-293T C167(1.03)  LDD1653  [7]
 LDCM0451  CL6 HEK-293T C167(0.86)  LDD1654  [7]
 LDCM0452  CL60 HEK-293T C167(1.00)  LDD1655  [7]
 LDCM0453  CL61 HEK-293T C167(1.06)  LDD1656  [7]
 LDCM0455  CL63 HEK-293T C167(0.99)  LDD1658  [7]
 LDCM0456  CL64 HEK-293T C167(1.01)  LDD1659  [7]
 LDCM0458  CL66 HEK-293T C167(0.93)  LDD1661  [7]
 LDCM0459  CL67 HEK-293T C167(1.18)  LDD1662  [7]
 LDCM0460  CL68 HEK-293T C167(1.10)  LDD1663  [7]
 LDCM0461  CL69 HEK-293T C167(1.09)  LDD1664  [7]
 LDCM0462  CL7 HEK-293T C167(1.19)  LDD1665  [7]
 LDCM0463  CL70 HEK-293T C167(1.13)  LDD1666  [7]
 LDCM0464  CL71 HEK-293T C167(1.05)  LDD1667  [7]
 LDCM0465  CL72 HEK-293T C167(0.95)  LDD1668  [7]
 LDCM0466  CL73 HEK-293T C167(1.05)  LDD1669  [7]
 LDCM0469  CL76 HEK-293T C167(0.98)  LDD1672  [7]
 LDCM0471  CL78 HEK-293T C167(0.96)  LDD1674  [7]
 LDCM0472  CL79 HEK-293T C167(1.19)  LDD1675  [7]
 LDCM0473  CL8 HEK-293T C167(0.90)  LDD1676  [7]
 LDCM0474  CL80 HEK-293T C167(1.25)  LDD1677  [7]
 LDCM0475  CL81 HEK-293T C167(1.08)  LDD1678  [7]
 LDCM0476  CL82 HEK-293T C167(1.17)  LDD1679  [7]
 LDCM0477  CL83 HEK-293T C167(1.11)  LDD1680  [7]
 LDCM0478  CL84 HEK-293T C167(1.08)  LDD1681  [7]
 LDCM0479  CL85 HEK-293T C167(1.05)  LDD1682  [7]
 LDCM0481  CL87 HEK-293T C167(0.94)  LDD1684  [7]
 LDCM0482  CL88 HEK-293T C167(1.08)  LDD1685  [7]
 LDCM0484  CL9 HEK-293T C167(1.07)  LDD1687  [7]
 LDCM0485  CL90 HEK-293T C167(0.86)  LDD1688  [7]
 LDCM0486  CL91 HEK-293T C167(1.31)  LDD1689  [7]
 LDCM0487  CL92 HEK-293T C167(1.02)  LDD1690  [7]
 LDCM0488  CL93 HEK-293T C167(1.00)  LDD1691  [7]
 LDCM0489  CL94 HEK-293T C167(1.18)  LDD1692  [7]
 LDCM0490  CL95 HEK-293T C167(0.83)  LDD1693  [7]
 LDCM0491  CL96 HEK-293T C167(1.03)  LDD1694  [7]
 LDCM0492  CL97 HEK-293T C167(0.93)  LDD1695  [7]
 LDCM0494  CL99 HEK-293T C167(0.99)  LDD1697  [7]
 LDCM0495  E2913 HEK-293T C167(0.96)  LDD1698  [7]
 LDCM0468  Fragment33 HEK-293T C167(1.04)  LDD1671  [7]
 LDCM0107  IAA HeLa N.A.  LDD0221  [6]
 LDCM0022  KB02 HEK-293T C167(0.93)  LDD1492  [7]
 LDCM0023  KB03 HEK-293T C167(0.95)  LDD1497  [7]
 LDCM0024  KB05 HEK-293T C167(0.96)  LDD1502  [7]
 LDCM0014  Panhematin HEK-293T 3.52  LDD0062  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
RNA-binding protein Musashi homolog 1 (MSI1) Musashi family O43347
Heterogeneous nuclear ribonucleoprotein F (HNRNPF) . P52597
Heterogeneous nuclear ribonucleoprotein H2 (HNRNPH2) . P55795

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
10 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
11 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
12 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.