Details of the Target

General Information of Target

Target ID LDTP05820
Target Name S-phase kinase-associated protein 2 (SKP2)
Gene Name SKP2
Gene ID 6502
Synonyms
FBXL1; S-phase kinase-associated protein 2; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; p45skp2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MHRKHLQEIPDLSSNVATSFTWGWDSSKTSELLSGMGVSALEKEEPDSENIPQELLSNLG
HPESPPRKRLKSKGSDKDFVIVRRPKLNRENFPGVSWDSLPDELLLGIFSCLCLPELLKV
SGVCKRWYRLASDESLWQTLDLTGKNLHPDVTGRLLSQGVIAFRCPRSFMDQPLAEHFSP
FRVQHMDLSNSVIEVSTLHGILSQCSKLQNLSLEGLRLSDPIVNTLAKNSNLVRLNLSGC
SGFSEFALQTLLSSCSRLDELNLSWCFDFTEKHVQVAVAHVSETITQLNLSGYRKNLQKS
DLSTLVRRCPNLVHLDLSDSVMLKNDCFQEFFQLNYLQHLSLSRCYDIIPETLLELGEIP
TLKTLQVFGIVPDGTLQLLKEALPHLQINCSHFTTIARPTIGNKKNQEIWGIKCRLTLQK
PSCL
Target Type
Literature-reported
Target Bioclass
Enzyme
Subcellular location
Cytoplasm
Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration.; Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus.
TTD ID
T80306
Uniprot ID
Q13309
DrugMap ID
TT5B2EO
Ensemble ID
ENST00000274254.9
HGNC ID
HGNC:10901

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 Deletion: p.P66HfsTer5 .
DU145 Insertion: p.N229KfsTer8 .
HCT15 SNV: p.S157P .
NCIH2286 Substitution: p.P173K .
OPM2 SNV: p.D301G .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C423(0.61)  LDD2109  [1]
NAIA_5
 Probe Info  		C205(0.07)  LDD2227  [2]
HPAP
 Probe Info  		4.41  LDD0062  [3]
Acrolein
 Probe Info  		N.A.  LDD0223  [4]
DBIA
 Probe Info  		C240(0.73); C255(0.73)  LDD0531  [5]
4-Iodoacetamidophenylacetylene
 Probe Info  		C345(0.00); C327(0.00); C205(0.00)  LDD0038  [6]
IA-alkyne
 Probe Info  		C345(0.00); C423(0.00); C327(0.00); C205(0.00)  LDD0036  [6]
Lodoacetamide azide
 Probe Info  		C345(0.00); C327(0.00); C423(0.00); C205(0.00)  LDD0037  [6]
IPM
 Probe Info  		N.A.  LDD0147  [7]
TFBX
 Probe Info  		N.A.  LDD0148  [7]
Phosphinate-6
 Probe Info  		C165(0.00); C124(0.00)  LDD0018  [8]
1c-yne
 Probe Info  		N.A.  LDD0228  [9]
AOyne
 Probe Info  		15.00  LDD0443  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C240(0.73); C255(0.73)  LDD0531  [5]
 LDCM0215  AC10 HCT 116 C240(0.64); C255(0.64)  LDD0532  [5]
 LDCM0216  AC100 PaTu 8988t C240(0.62); C255(0.62)  LDD1095  [5]
 LDCM0217  AC101 PaTu 8988t C240(0.68); C255(0.68)  LDD1096  [5]
 LDCM0218  AC102 PaTu 8988t C240(1.04); C255(1.04)  LDD1097  [5]
 LDCM0219  AC103 PaTu 8988t C240(0.59); C255(0.59)  LDD1098  [5]
 LDCM0220  AC104 PaTu 8988t C240(0.76); C255(0.76)  LDD1099  [5]
 LDCM0221  AC105 PaTu 8988t C240(0.69); C255(0.69)  LDD1100  [5]
 LDCM0222  AC106 PaTu 8988t C240(0.48); C255(0.48)  LDD1101  [5]
 LDCM0223  AC107 PaTu 8988t C240(0.87); C255(0.87)  LDD1102  [5]
 LDCM0224  AC108 PaTu 8988t C240(0.59); C255(0.59)  LDD1103  [5]
 LDCM0225  AC109 PaTu 8988t C240(0.59); C255(0.59)  LDD1104  [5]
 LDCM0226  AC11 HCT 116 C240(0.61); C255(0.61)  LDD0543  [5]
 LDCM0227  AC110 PaTu 8988t C240(0.78); C255(0.78)  LDD1106  [5]
 LDCM0228  AC111 PaTu 8988t C240(0.62); C255(0.62)  LDD1107  [5]
 LDCM0229  AC112 PaTu 8988t C240(0.55); C255(0.55)  LDD1108  [5]
 LDCM0237  AC12 HCT 116 C240(0.81); C255(0.81)  LDD0554  [5]
 LDCM0246  AC128 HEK-293T C255(0.97)  LDD0844  [5]
 LDCM0247  AC129 HEK-293T C255(1.08)  LDD0845  [5]
 LDCM0249  AC130 HEK-293T C255(1.06)  LDD0847  [5]
 LDCM0250  AC131 HEK-293T C255(1.02)  LDD0848  [5]
 LDCM0251  AC132 HEK-293T C255(1.11)  LDD0849  [5]
 LDCM0252  AC133 HEK-293T C255(1.16)  LDD0850  [5]
 LDCM0253  AC134 HEK-293T C255(1.30)  LDD0851  [5]
 LDCM0254  AC135 HEK-293T C255(1.18)  LDD0852  [5]
 LDCM0255  AC136 HEK-293T C255(1.20)  LDD0853  [5]
 LDCM0256  AC137 HEK-293T C255(1.09)  LDD0854  [5]
 LDCM0257  AC138 HEK-293T C255(1.21)  LDD0855  [5]
 LDCM0258  AC139 HEK-293T C255(1.22)  LDD0856  [5]
 LDCM0259  AC14 HCT 116 C240(0.65); C255(0.65)  LDD0576  [5]
 LDCM0260  AC140 HEK-293T C255(1.50)  LDD0858  [5]
 LDCM0261  AC141 HEK-293T C255(1.43)  LDD0859  [5]
 LDCM0262  AC142 HEK-293T C255(1.19)  LDD0860  [5]
 LDCM0263  AC143 HCT 116 C240(0.95); C255(0.95)  LDD0580  [5]
 LDCM0264  AC144 HCT 116 C240(0.56); C255(0.56)  LDD0581  [5]
 LDCM0265  AC145 HCT 116 C240(0.71); C255(0.71)  LDD0582  [5]
 LDCM0266  AC146 HCT 116 C240(0.50); C255(0.50)  LDD0583  [5]
 LDCM0267  AC147 HCT 116 C240(0.52); C255(0.52)  LDD0584  [5]
 LDCM0268  AC148 HCT 116 C240(0.75); C255(0.75)  LDD0585  [5]
 LDCM0269  AC149 HCT 116 C240(0.55); C255(0.55)  LDD0586  [5]
 LDCM0270  AC15 HCT 116 C240(0.54); C255(0.54)  LDD0587  [5]
 LDCM0271  AC150 HCT 116 C240(0.70); C255(0.70)  LDD0588  [5]
 LDCM0272  AC151 HCT 116 C240(0.74); C255(0.74)  LDD0589  [5]
 LDCM0273  AC152 HCT 116 C240(0.64); C255(0.64)  LDD0590  [5]
 LDCM0274  AC153 HCT 116 C240(0.61); C255(0.61)  LDD0591  [5]
 LDCM0621  AC154 HCT 116 C240(0.55); C255(0.55)  LDD2158  [5]
 LDCM0622  AC155 HCT 116 C240(0.58); C255(0.58)  LDD2159  [5]
 LDCM0623  AC156 HCT 116 C240(1.27); C255(1.27)  LDD2160  [5]
 LDCM0624  AC157 HCT 116 C240(1.75); C255(1.75)  LDD2161  [5]
 LDCM0276  AC17 HEK-293T C309(1.03); C240(1.15)  LDD1515  [11]
 LDCM0277  AC18 HEK-293T C309(1.02); C240(1.10)  LDD1516  [11]
 LDCM0278  AC19 HEK-293T C309(1.07); C266(0.98); C240(0.97)  LDD1517  [11]
 LDCM0279  AC2 HCT 116 C240(0.62); C255(0.62)  LDD0596  [5]
 LDCM0280  AC20 HEK-293T C309(0.93); C266(1.07); C240(1.06)  LDD1519  [11]
 LDCM0281  AC21 HEK-293T C309(0.97); C240(0.96)  LDD1520  [11]
 LDCM0282  AC22 HEK-293T C309(0.99); C266(1.04); C255(0.80)  LDD1521  [11]
 LDCM0283  AC23 HEK-293T C309(1.04); C266(1.05); C240(1.10); C113(0.92)  LDD1522  [11]
 LDCM0284  AC24 HEK-293T C309(1.07); C266(0.91); C345(1.00); C240(1.11)  LDD1523  [11]
 LDCM0285  AC25 HCT 116 C240(0.99); C255(0.99)  LDD0602  [5]
 LDCM0286  AC26 HCT 116 C240(0.73); C255(0.73)  LDD0603  [5]
 LDCM0287  AC27 HCT 116 C240(0.67); C255(0.67)  LDD0604  [5]
 LDCM0288  AC28 HCT 116 C240(0.76); C255(0.76)  LDD0605  [5]
 LDCM0289  AC29 HCT 116 C240(0.74); C255(0.74)  LDD0606  [5]
 LDCM0290  AC3 HCT 116 C240(0.68); C255(0.68)  LDD0607  [5]
 LDCM0291  AC30 HCT 116 C240(0.53); C255(0.53)  LDD0608  [5]
 LDCM0292  AC31 HCT 116 C240(0.77); C255(0.77)  LDD0609  [5]
 LDCM0293  AC32 HCT 116 C240(0.56); C255(0.56)  LDD0610  [5]
 LDCM0294  AC33 HCT 116 C240(0.63); C255(0.63)  LDD0611  [5]
 LDCM0295  AC34 HCT 116 C240(0.53); C255(0.53)  LDD0612  [5]
 LDCM0296  AC35 HEK-293T C255(0.80)  LDD0894  [5]
 LDCM0297  AC36 HEK-293T C255(0.81)  LDD0895  [5]
 LDCM0298  AC37 HEK-293T C255(0.84)  LDD0896  [5]
 LDCM0299  AC38 HEK-293T C255(0.77)  LDD0897  [5]
 LDCM0300  AC39 HEK-293T C255(0.84)  LDD0898  [5]
 LDCM0301  AC4 HCT 116 C240(0.49); C255(0.49)  LDD0618  [5]
 LDCM0302  AC40 HEK-293T C255(0.90)  LDD0900  [5]
 LDCM0303  AC41 HEK-293T C255(0.82)  LDD0901  [5]
 LDCM0304  AC42 HEK-293T C255(0.98)  LDD0902  [5]
 LDCM0305  AC43 HEK-293T C255(1.00)  LDD0903  [5]
 LDCM0306  AC44 HEK-293T C255(1.10)  LDD0904  [5]
 LDCM0307  AC45 HEK-293T C255(0.92)  LDD0905  [5]
 LDCM0308  AC46 PaTu 8988t C240(0.62); C255(0.62)  LDD1187  [5]
 LDCM0309  AC47 PaTu 8988t C240(0.75); C255(0.75)  LDD1188  [5]
 LDCM0310  AC48 PaTu 8988t C240(0.54); C255(0.54)  LDD1189  [5]
 LDCM0311  AC49 PaTu 8988t C240(0.50); C255(0.50)  LDD1190  [5]
 LDCM0312  AC5 HCT 116 C240(0.50); C255(0.50)  LDD0629  [5]
 LDCM0313  AC50 PaTu 8988t C240(0.52); C255(0.52)  LDD1192  [5]
 LDCM0314  AC51 PaTu 8988t C240(0.69); C255(0.69)  LDD1193  [5]
 LDCM0315  AC52 PaTu 8988t C240(0.50); C255(0.50)  LDD1194  [5]
 LDCM0316  AC53 PaTu 8988t C240(0.58); C255(0.58)  LDD1195  [5]
 LDCM0317  AC54 PaTu 8988t C240(0.88); C255(0.88)  LDD1196  [5]
 LDCM0318  AC55 PaTu 8988t C240(0.43); C255(0.43)  LDD1197  [5]
 LDCM0319  AC56 PaTu 8988t C240(1.18); C255(1.18)  LDD1198  [5]
 LDCM0320  AC57 HEK-293T C255(1.10)  LDD0918  [5]
 LDCM0321  AC58 HEK-293T C255(1.01)  LDD0919  [5]
 LDCM0322  AC59 HEK-293T C255(0.94)  LDD0920  [5]
 LDCM0323  AC6 HCT 116 C240(0.55); C255(0.55)  LDD0640  [5]
 LDCM0324  AC60 HEK-293T C255(1.13)  LDD0922  [5]
 LDCM0325  AC61 HEK-293T C255(1.12)  LDD0923  [5]
 LDCM0326  AC62 HEK-293T C255(0.90)  LDD0924  [5]
 LDCM0327  AC63 HEK-293T C255(1.06)  LDD0925  [5]
 LDCM0328  AC64 HEK-293T C255(0.85)  LDD0926  [5]
 LDCM0329  AC65 HEK-293T C255(1.06)  LDD0927  [5]
 LDCM0330  AC66 HEK-293T C255(1.04)  LDD0928  [5]
 LDCM0331  AC67 HEK-293T C255(0.95)  LDD0929  [5]
 LDCM0332  AC68 HEK-293T C255(1.15)  LDD0930  [5]
 LDCM0333  AC69 HEK-293T C255(1.23)  LDD0931  [5]
 LDCM0334  AC7 HCT 116 C240(0.77); C255(0.77)  LDD0651  [5]
 LDCM0335  AC70 HEK-293T C255(1.35)  LDD0933  [5]
 LDCM0336  AC71 HEK-293T C255(1.42)  LDD0934  [5]
 LDCM0337  AC72 HEK-293T C255(1.43)  LDD0935  [5]
 LDCM0338  AC73 HEK-293T C255(1.55)  LDD0936  [5]
 LDCM0339  AC74 HEK-293T C255(1.48)  LDD0937  [5]
 LDCM0340  AC75 HEK-293T C255(1.56)  LDD0938  [5]
 LDCM0341  AC76 HEK-293T C255(1.33)  LDD0939  [5]
 LDCM0342  AC77 HEK-293T C255(1.64)  LDD0940  [5]
 LDCM0343  AC78 HEK-293T C255(1.40)  LDD0941  [5]
 LDCM0344  AC79 HEK-293T C255(1.28)  LDD0942  [5]
 LDCM0345  AC8 HCT 116 C240(0.60); C255(0.60)  LDD0662  [5]
 LDCM0346  AC80 HEK-293T C255(1.47)  LDD0944  [5]
 LDCM0347  AC81 HEK-293T C255(1.55)  LDD0945  [5]
 LDCM0348  AC82 HEK-293T C255(1.82)  LDD0946  [5]
 LDCM0365  AC98 PaTu 8988t C240(0.81); C255(0.81)  LDD1244  [5]
 LDCM0366  AC99 PaTu 8988t C240(0.64); C255(0.64)  LDD1245  [5]
 LDCM0248  AKOS034007472 HCT 116 C240(1.04); C255(1.04)  LDD0565  [5]
 LDCM0356  AKOS034007680 HCT 116 C240(0.71); C255(0.71)  LDD0673  [5]
 LDCM0275  AKOS034007705 HCT 116 C240(0.65); C255(0.65)  LDD0592  [5]
 LDCM0632  CL-Sc Hep-G2 C205(0.07)  LDD2227  [2]
 LDCM0367  CL1 HCT 116 C240(1.58); C255(1.58)  LDD0684  [5]
 LDCM0368  CL10 HCT 116 C240(1.19); C255(1.19)  LDD0685  [5]
 LDCM0369  CL100 HCT 116 C240(0.53); C255(0.53)  LDD0686  [5]
 LDCM0370  CL101 HCT 116 C240(0.45); C255(0.45)  LDD0687  [5]
 LDCM0371  CL102 HCT 116 C240(0.79); C255(0.79)  LDD0688  [5]
 LDCM0372  CL103 HCT 116 C240(1.10); C255(1.10)  LDD0689  [5]
 LDCM0373  CL104 HCT 116 C240(0.89); C255(0.89)  LDD0690  [5]
 LDCM0374  CL105 HEK-293T C309(1.05); C266(0.93); C240(1.09)  LDD1578  [11]
 LDCM0375  CL106 HEK-293T C309(1.02); C266(0.95); C240(0.93)  LDD1579  [11]
 LDCM0376  CL107 HEK-293T C309(1.05); C266(1.02); C240(0.93)  LDD1580  [11]
 LDCM0377  CL108 HEK-293T C309(0.95); C240(0.87)  LDD1581  [11]
 LDCM0378  CL109 HEK-293T C309(1.00); C266(0.95); C240(1.15)  LDD1582  [11]
 LDCM0379  CL11 HCT 116 C240(1.12); C255(1.12)  LDD0696  [5]
 LDCM0380  CL110 HEK-293T C309(0.96); C266(0.85); C240(1.00)  LDD1584  [11]
 LDCM0381  CL111 HEK-293T C309(0.96); C266(0.98); C240(1.01)  LDD1585  [11]
 LDCM0382  CL112 HCT 116 C240(1.02); C255(1.02)  LDD0699  [5]
 LDCM0383  CL113 HCT 116 C240(0.57); C255(0.57)  LDD0700  [5]
 LDCM0384  CL114 HCT 116 C240(0.72); C255(0.72)  LDD0701  [5]
 LDCM0385  CL115 HCT 116 C240(0.57); C255(0.57)  LDD0702  [5]
 LDCM0386  CL116 HCT 116 C240(0.61); C255(0.61)  LDD0703  [5]
 LDCM0387  CL117 HEK-293T C255(0.95)  LDD0985  [5]
 LDCM0388  CL118 HEK-293T C255(0.80)  LDD0986  [5]
 LDCM0389  CL119 HEK-293T C255(0.92)  LDD0987  [5]
 LDCM0390  CL12 HCT 116 C240(1.09); C255(1.09)  LDD0707  [5]
 LDCM0391  CL120 HEK-293T C255(0.86)  LDD0989  [5]
 LDCM0392  CL121 PaTu 8988t C240(0.53); C255(0.53)  LDD1271  [5]
 LDCM0393  CL122 PaTu 8988t C240(0.76); C255(0.76)  LDD1272  [5]
 LDCM0394  CL123 PaTu 8988t C240(0.55); C255(0.55)  LDD1273  [5]
 LDCM0395  CL124 PaTu 8988t C240(0.53); C255(0.53)  LDD1274  [5]
 LDCM0396  CL125 HEK-293T C255(1.02)  LDD0994  [5]
 LDCM0397  CL126 HEK-293T C255(1.06)  LDD0995  [5]
 LDCM0398  CL127 HEK-293T C255(1.07)  LDD0996  [5]
 LDCM0399  CL128 HEK-293T C255(0.82)  LDD0997  [5]
 LDCM0400  CL13 HCT 116 C240(0.89); C255(0.89)  LDD0717  [5]
 LDCM0401  CL14 HCT 116 C240(1.10); C255(1.10)  LDD0718  [5]
 LDCM0402  CL15 HCT 116 C240(1.47); C255(1.47)  LDD0719  [5]
 LDCM0403  CL16 HCT 116 C240(0.86); C255(0.86)  LDD0720  [5]
 LDCM0404  CL17 HCT 116 C240(1.03); C255(1.03)  LDD0721  [5]
 LDCM0405  CL18 HCT 116 C240(0.88); C255(0.88)  LDD0722  [5]
 LDCM0406  CL19 HCT 116 C240(0.91); C255(0.91)  LDD0723  [5]
 LDCM0407  CL2 HCT 116 C240(1.12); C255(1.12)  LDD0724  [5]
 LDCM0408  CL20 HCT 116 C240(0.79); C255(0.79)  LDD0725  [5]
 LDCM0409  CL21 HCT 116 C240(0.94); C255(0.94)  LDD0726  [5]
 LDCM0410  CL22 HCT 116 C240(1.18); C255(1.18)  LDD0727  [5]
 LDCM0411  CL23 HCT 116 C240(0.78); C255(0.78)  LDD0728  [5]
 LDCM0412  CL24 HCT 116 C240(0.93); C255(0.93)  LDD0729  [5]
 LDCM0413  CL25 HCT 116 C240(0.90); C255(0.90)  LDD0730  [5]
 LDCM0414  CL26 HCT 116 C240(0.90); C255(0.90)  LDD0731  [5]
 LDCM0415  CL27 HCT 116 C240(0.78); C255(0.78)  LDD0732  [5]
 LDCM0416  CL28 HCT 116 C240(1.00); C255(1.00)  LDD0733  [5]
 LDCM0417  CL29 HCT 116 C240(0.90); C255(0.90)  LDD0734  [5]
 LDCM0418  CL3 HCT 116 C240(0.76); C255(0.76)  LDD0735  [5]
 LDCM0419  CL30 HCT 116 C240(0.88); C255(0.88)  LDD0736  [5]
 LDCM0420  CL31 PaTu 8988t C240(0.56); C255(0.56)  LDD1299  [5]
 LDCM0421  CL32 PaTu 8988t C240(0.49); C255(0.49)  LDD1300  [5]
 LDCM0422  CL33 PaTu 8988t C240(0.57); C255(0.57)  LDD1301  [5]
 LDCM0423  CL34 PaTu 8988t C240(0.60); C255(0.60)  LDD1302  [5]
 LDCM0424  CL35 PaTu 8988t C240(0.57); C255(0.57)  LDD1303  [5]
 LDCM0425  CL36 PaTu 8988t C240(0.68); C255(0.68)  LDD1304  [5]
 LDCM0426  CL37 PaTu 8988t C240(0.58); C255(0.58)  LDD1305  [5]
 LDCM0428  CL39 PaTu 8988t C240(0.62); C255(0.62)  LDD1307  [5]
 LDCM0429  CL4 HCT 116 C240(0.92); C255(0.92)  LDD0746  [5]
 LDCM0430  CL40 PaTu 8988t C240(0.46); C255(0.46)  LDD1309  [5]
 LDCM0431  CL41 PaTu 8988t C240(0.52); C255(0.52)  LDD1310  [5]
 LDCM0432  CL42 PaTu 8988t C240(0.68); C255(0.68)  LDD1311  [5]
 LDCM0433  CL43 PaTu 8988t C240(0.91); C255(0.91)  LDD1312  [5]
 LDCM0434  CL44 PaTu 8988t C240(0.58); C255(0.58)  LDD1313  [5]
 LDCM0435  CL45 PaTu 8988t C240(0.55); C255(0.55)  LDD1314  [5]
 LDCM0436  CL46 PaTu 8988t C240(0.74); C255(0.74)  LDD1315  [5]
 LDCM0437  CL47 PaTu 8988t C240(0.91); C255(0.91)  LDD1316  [5]
 LDCM0438  CL48 PaTu 8988t C240(0.67); C255(0.67)  LDD1317  [5]
 LDCM0439  CL49 PaTu 8988t C240(0.63); C255(0.63)  LDD1318  [5]
 LDCM0440  CL5 HCT 116 C240(0.89); C255(0.89)  LDD0757  [5]
 LDCM0441  CL50 PaTu 8988t C240(0.74); C255(0.74)  LDD1320  [5]
 LDCM0442  CL51 PaTu 8988t C240(0.64); C255(0.64)  LDD1321  [5]
 LDCM0443  CL52 PaTu 8988t C240(0.62); C255(0.62)  LDD1322  [5]
 LDCM0444  CL53 PaTu 8988t C240(0.63); C255(0.63)  LDD1323  [5]
 LDCM0445  CL54 PaTu 8988t C240(0.85); C255(0.85)  LDD1324  [5]
 LDCM0446  CL55 PaTu 8988t C240(0.58); C255(0.58)  LDD1325  [5]
 LDCM0447  CL56 PaTu 8988t C240(0.65); C255(0.65)  LDD1326  [5]
 LDCM0448  CL57 PaTu 8988t C240(0.69); C255(0.69)  LDD1327  [5]
 LDCM0449  CL58 PaTu 8988t C240(0.64); C255(0.64)  LDD1328  [5]
 LDCM0450  CL59 PaTu 8988t C240(0.87); C255(0.87)  LDD1329  [5]
 LDCM0451  CL6 HCT 116 C240(1.27); C255(1.27)  LDD0768  [5]
 LDCM0452  CL60 PaTu 8988t C240(0.73); C255(0.73)  LDD1331  [5]
 LDCM0453  CL61 HCT 116 C240(0.59); C255(0.59)  LDD0770  [5]
 LDCM0454  CL62 HCT 116 C240(0.50); C255(0.50)  LDD0771  [5]
 LDCM0455  CL63 HCT 116 C240(0.45); C255(0.45)  LDD0772  [5]
 LDCM0456  CL64 HCT 116 C240(0.49); C255(0.49)  LDD0773  [5]
 LDCM0457  CL65 HCT 116 C240(0.40); C255(0.40)  LDD0774  [5]
 LDCM0458  CL66 HCT 116 C240(0.47); C255(0.47)  LDD0775  [5]
 LDCM0459  CL67 HCT 116 C240(0.51); C255(0.51)  LDD0776  [5]
 LDCM0460  CL68 HCT 116 C240(0.40); C255(0.40)  LDD0777  [5]
 LDCM0461  CL69 HCT 116 C240(0.89); C255(0.89)  LDD0778  [5]
 LDCM0462  CL7 HCT 116 C240(1.00); C255(1.00)  LDD0779  [5]
 LDCM0463  CL70 HCT 116 C240(0.50); C255(0.50)  LDD0780  [5]
 LDCM0464  CL71 HCT 116 C240(0.36); C255(0.36)  LDD0781  [5]
 LDCM0465  CL72 HCT 116 C240(1.13); C255(1.13)  LDD0782  [5]
 LDCM0466  CL73 HCT 116 C240(0.65); C255(0.65)  LDD0783  [5]
 LDCM0467  CL74 HCT 116 C240(0.40); C255(0.40)  LDD0784  [5]
 LDCM0469  CL76 HCT 116 C240(1.20); C255(1.20)  LDD0786  [5]
 LDCM0470  CL77 HCT 116 C240(1.19); C255(1.19)  LDD0787  [5]
 LDCM0471  CL78 HCT 116 C240(0.92); C255(0.92)  LDD0788  [5]
 LDCM0472  CL79 HCT 116 C240(1.02); C255(1.02)  LDD0789  [5]
 LDCM0473  CL8 HCT 116 C240(1.28); C255(1.28)  LDD0790  [5]
 LDCM0474  CL80 HCT 116 C240(1.26); C255(1.26)  LDD0791  [5]
 LDCM0475  CL81 HCT 116 C240(0.61); C255(0.61)  LDD0792  [5]
 LDCM0476  CL82 HCT 116 C240(0.98); C255(0.98)  LDD0793  [5]
 LDCM0477  CL83 HCT 116 C240(1.33); C255(1.33)  LDD0794  [5]
 LDCM0478  CL84 HCT 116 C240(1.19); C255(1.19)  LDD0795  [5]
 LDCM0479  CL85 HCT 116 C240(0.67); C255(0.67)  LDD0796  [5]
 LDCM0480  CL86 HCT 116 C240(1.16); C255(1.16)  LDD0797  [5]
 LDCM0481  CL87 HCT 116 C240(1.12); C255(1.12)  LDD0798  [5]
 LDCM0482  CL88 HCT 116 C240(1.01); C255(1.01)  LDD0799  [5]
 LDCM0483  CL89 HCT 116 C240(1.55); C255(1.55)  LDD0800  [5]
 LDCM0484  CL9 HCT 116 C240(1.24); C255(1.24)  LDD0801  [5]
 LDCM0485  CL90 HCT 116 C240(1.21); C255(1.21)  LDD0802  [5]
 LDCM0486  CL91 HCT 116 C240(0.64); C255(0.64)  LDD0803  [5]
 LDCM0487  CL92 HCT 116 C240(0.67); C255(0.67)  LDD0804  [5]
 LDCM0488  CL93 HCT 116 C240(0.66); C255(0.66)  LDD0805  [5]
 LDCM0489  CL94 HCT 116 C240(0.53); C255(0.53)  LDD0806  [5]
 LDCM0490  CL95 HCT 116 C240(0.57); C255(0.57)  LDD0807  [5]
 LDCM0491  CL96 HCT 116 C240(0.49); C255(0.49)  LDD0808  [5]
 LDCM0492  CL97 HCT 116 C240(0.62); C255(0.62)  LDD0809  [5]
 LDCM0493  CL98 HCT 116 C240(0.46); C255(0.46)  LDD0810  [5]
 LDCM0494  CL99 HCT 116 C240(0.50); C255(0.50)  LDD0811  [5]
 LDCM0495  E2913 HEK-293T C309(1.00); C266(1.04); C240(0.98)  LDD1698  [11]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C423(5.44)  LDD1702  [1]
 LDCM0468  Fragment33 HCT 116 C240(0.49); C255(0.49)  LDD0785  [5]
 LDCM0427  Fragment51 PaTu 8988t C240(0.57); C255(0.57)  LDD1306  [5]
 LDCM0022  KB02 HEK-293T C309(0.96); C205(1.12); C345(0.99); C240(1.34)  LDD1492  [11]
 LDCM0023  KB03 HEK-293T C309(1.07); C205(0.95); C345(0.97); C240(1.29)  LDD1497  [11]
 LDCM0024  KB05 NCI-N87 C345(1.58)  LDD3364  [12]
 LDCM0109  NEM HeLa N.A.  LDD0223  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C423(0.61)  LDD2109  [1]
 LDCM0627  NUDT7-COV-1 HEK-293T C124(0.49)  LDD2206  [13]
 LDCM0628  OTUB2-COV-1 HEK-293T C124(1.10)  LDD2207  [13]
 LDCM0014  Panhematin HEK-293T 4.41  LDD0062  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone-lysine N-methyltransferase 2A (KMT2A) Histone-lysine methyltransferase family Q03164
Cullin-1 (CUL1) Cullin family Q13616
Dual specificity protein phosphatase 1 (DUSP1) Protein-tyrosine phosphatase family P28562
E3 ubiquitin-protein ligase RBX1 (RBX1) RING-box family P62877
NAD-dependent protein deacetylase sirtuin-3, mitochondrial (SIRT3) Sirtuin family Q9NTG7
S-phase kinase-associated protein 1 (SKP1) SKP1 family P63208
Fizzy-related protein homolog (FZR1) WD repeat CDC20/Fizzy family Q9UM11
Histone acetyltransferase p300 (EP300) . Q09472
S-phase kinase-associated protein 2 (SKP2) . Q13309
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Prohibitin 1 (PHB1) Prohibitin family P35232
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Myc proto-oncogene protein (MYC) . P01106
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclin-dependent kinase inhibitor 1 (CDKN1A) CDI family P38936
Cyclin-dependent kinase inhibitor 1B (CDKN1B) CDI family P46527
Cyclin-dependent kinases regulatory subunit 1 (CKS1B) CKS family P61024
Origin recognition complex subunit 1 (ORC1) ORC1 family Q13415

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
9 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
10 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
11 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
12 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
13 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.