Details of the Target

General Information of Target

Target ID LDTP10216
Target Name RNA-binding protein Musashi homolog 2 (MSI2)
Gene Name MSI2
Gene ID 124540
Synonyms
RNA-binding protein Musashi homolog 2; Musashi-2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MANEAYPCPCDIGHRLEYGGLGREVQVEHIKAYVTKSPVDAGKAVIVIQDIFGWQLPNTR
YIADMISGNGYTTIVPDFFVGQEPWDPSGDWSIFPEWLKTRNAQKIDREISAILKYLKQQ
CHAQKIGIVGFCWGGTAVHHLMMKYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDV
VIPLKDVSLLTQKLKEHCKVEYQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEW
LNKYM
Target Type
Discontinued
Target Bioclass
Other
Family
Musashi family
Subcellular location
Cytoplasm
Function RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
TTD ID
T35817
Uniprot ID
Q96DH6
DrugMap ID
TTTXQF6
Ensemble ID
ENST00000284073.7
HGNC ID
HGNC:18585

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.H171R DBIA    Probe Info 
FADU SNV: p.E174K .
HT115 SNV: p.F24C DBIA    Probe Info 
KMCH1 SNV: p.S236T .
REH SNV: p.R201Q DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y40(16.85)  LDD0257  [1]
DBIA
 Probe Info  		C64(1.24)  LDD3310  [2]
HPAP
 Probe Info  		3.53  LDD0062  [3]
Acrolein
 Probe Info  		N.A.  LDD0223  [4]
5E-2FA
 Probe Info  		N.A.  LDD2235  [5]
m-APA
 Probe Info  		H171(0.00); H83(0.00)  LDD2231  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0167  [6]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [7]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [7]
BTD
 Probe Info  		N.A.  LDD0004  [8]
NAIA_5
 Probe Info  		N.A.  LDD2223  [9]
PAL-AfBPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C092
 Probe Info  		18.00  LDD1783  [10]
FFF probe11
 Probe Info  		20.00  LDD0471  [11]
FFF probe13
 Probe Info  		20.00  LDD0475  [11]
FFF probe2
 Probe Info  		20.00  LDD0463  [11]
FFF probe3
 Probe Info  		20.00  LDD0464  [11]
JN0003
 Probe Info  		20.00  LDD0469  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 22RV1 C64(1.46)  LDD2243  [2]
 LDCM0023  KB03 22RV1 C64(1.84)  LDD2660  [2]
 LDCM0024  KB05 COLO792 C64(1.24)  LDD3310  [2]
 LDCM0109  NEM HeLa N.A.  LDD0223  [4]
 LDCM0014  Panhematin HEK-293T 3.53  LDD0062  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein MOX-2 (MEOX2) . P50222
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
RNA-binding protein Musashi homolog 2 (MSI2) Musashi family Q96DH6
Heterogeneous nuclear ribonucleoprotein H (HNRNPH1) . P31943
Heterogeneous nuclear ribonucleoprotein H2 (HNRNPH2) . P55795

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
7 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
11 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.