Details of the Target

General Information of Target

Target ID LDTP10927
Target Name COP9 signalosome complex subunit 8 (COPS8)
Gene Name COPS8
Gene ID 10920
Synonyms
CSN8; COP9 signalosome complex subunit 8; SGN8; Signalosome subunit 8; COP9 homolog; hCOP9; JAB1-containing signalosome subunit 8
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MYVSYLLDKDVSMYPSSVRHSGGLNLAPQNFVSPPQYPDYGGYHVAAAAAAAANLDSAQS
PGPSWPAAYGAPLREDWNGYAPGGAAAAANAVAHGLNGGSPAAAMGYSSPADYHPHHHPH
HHPHHPAAAPSCASGLLQTLNPGPPGPAATAAAEQLSPGGQRRNLCEWMRKPAQQSLGSQ
VKTRTKDKYRVVYTDHQRLELEKEFHYSRYITIRRKAELAATLGLSERQVKIWFQNRRAK
ERKINKKKLQQQQQQQPPQPPPPPPQPPQPQPGPLRSVPEPLSPVSSLQASVPGSVPGVL
GPTGGVLNPTVTQ
Target Bioclass
Other
Family
CSN8 family
Subcellular location
Cytoplasm
Function
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Uniprot ID
Q99627
Ensemble ID
ENST00000354371.7
HGNC ID
HGNC:24335
ChEMBL ID
CHEMBL4296015

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.V168F .
JURKAT SNV: p.Y44H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K178(3.22)  LDD0277  [1]
DBIA
 Probe Info  		C20(1.01)  LDD1508  [2]
m-APA
 Probe Info  		N.A.  LDD2231  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [4]
1d-yne
 Probe Info  		N.A.  LDD0356  [5]
1c-yne
 Probe Info  		N.A.  LDD0228  [5]
Acrolein
 Probe Info  		N.A.  LDD0217  [6]
NAIA_5
 Probe Info  		N.A.  LDD2223  [7]
PAL-AfBPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C027
 Probe Info  		5.35  LDD1733  [8]
C106
 Probe Info  		18.51  LDD1793  [8]
C244
 Probe Info  		14.93  LDD1917  [8]
C284
 Probe Info  		22.78  LDD1954  [8]
C310
 Probe Info  		7.94  LDD1977  [8]
C362
 Probe Info  		34.30  LDD2023  [8]
C363
 Probe Info  		15.67  LDD2024  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C20(1.01)  LDD1508  [2]
 LDCM0226  AC11 HEK-293T C20(1.08)  LDD1509  [2]
 LDCM0237  AC12 HEK-293T C20(1.07)  LDD1510  [2]
 LDCM0259  AC14 HEK-293T C20(1.13)  LDD1512  [2]
 LDCM0270  AC15 HEK-293T C20(0.85)  LDD1513  [2]
 LDCM0277  AC18 HEK-293T C20(1.22)  LDD1516  [2]
 LDCM0278  AC19 HEK-293T C20(0.86)  LDD1517  [2]
 LDCM0279  AC2 HEK-293T C20(1.06)  LDD1518  [2]
 LDCM0280  AC20 HEK-293T C20(1.22)  LDD1519  [2]
 LDCM0281  AC21 HEK-293T C20(1.02)  LDD1520  [2]
 LDCM0282  AC22 HEK-293T C20(0.99)  LDD1521  [2]
 LDCM0283  AC23 HEK-293T C20(0.92)  LDD1522  [2]
 LDCM0284  AC24 HEK-293T C20(1.19)  LDD1523  [2]
 LDCM0286  AC26 HEK-293T C20(0.94)  LDD1525  [2]
 LDCM0287  AC27 HEK-293T C20(0.95)  LDD1526  [2]
 LDCM0288  AC28 HEK-293T C20(1.13)  LDD1527  [2]
 LDCM0289  AC29 HEK-293T C20(1.13)  LDD1528  [2]
 LDCM0290  AC3 HEK-293T C20(1.13)  LDD1529  [2]
 LDCM0291  AC30 HEK-293T C20(0.93)  LDD1530  [2]
 LDCM0292  AC31 HEK-293T C20(0.96)  LDD1531  [2]
 LDCM0293  AC32 HEK-293T C20(1.06)  LDD1532  [2]
 LDCM0295  AC34 HEK-293T C20(1.39)  LDD1534  [2]
 LDCM0296  AC35 HEK-293T C20(0.77)  LDD1535  [2]
 LDCM0297  AC36 HEK-293T C20(1.14)  LDD1536  [2]
 LDCM0298  AC37 HEK-293T C20(1.10)  LDD1537  [2]
 LDCM0299  AC38 HEK-293T C20(0.88)  LDD1538  [2]
 LDCM0300  AC39 HEK-293T C20(0.98)  LDD1539  [2]
 LDCM0301  AC4 HEK-293T C20(1.08)  LDD1540  [2]
 LDCM0302  AC40 HEK-293T C20(1.01)  LDD1541  [2]
 LDCM0304  AC42 HEK-293T C20(0.98)  LDD1543  [2]
 LDCM0305  AC43 HEK-293T C20(1.05)  LDD1544  [2]
 LDCM0306  AC44 HEK-293T C20(0.99)  LDD1545  [2]
 LDCM0307  AC45 HEK-293T C20(0.81)  LDD1546  [2]
 LDCM0308  AC46 HEK-293T C20(1.09)  LDD1547  [2]
 LDCM0309  AC47 HEK-293T C20(0.92)  LDD1548  [2]
 LDCM0310  AC48 HEK-293T C20(1.27)  LDD1549  [2]
 LDCM0312  AC5 HEK-293T C20(1.17)  LDD1551  [2]
 LDCM0313  AC50 HEK-293T C20(1.23)  LDD1552  [2]
 LDCM0314  AC51 HEK-293T C20(1.28)  LDD1553  [2]
 LDCM0315  AC52 HEK-293T C20(0.93)  LDD1554  [2]
 LDCM0316  AC53 HEK-293T C20(0.97)  LDD1555  [2]
 LDCM0317  AC54 HEK-293T C20(0.85)  LDD1556  [2]
 LDCM0318  AC55 HEK-293T C20(0.73)  LDD1557  [2]
 LDCM0319  AC56 HEK-293T C20(1.09)  LDD1558  [2]
 LDCM0321  AC58 HEK-293T C20(1.22)  LDD1560  [2]
 LDCM0322  AC59 HEK-293T C20(1.17)  LDD1561  [2]
 LDCM0323  AC6 HEK-293T C20(0.93)  LDD1562  [2]
 LDCM0324  AC60 HEK-293T C20(0.97)  LDD1563  [2]
 LDCM0325  AC61 HEK-293T C20(1.61)  LDD1564  [2]
 LDCM0326  AC62 HEK-293T C20(1.05)  LDD1565  [2]
 LDCM0327  AC63 HEK-293T C20(1.21)  LDD1566  [2]
 LDCM0328  AC64 HEK-293T C20(1.28)  LDD1567  [2]
 LDCM0334  AC7 HEK-293T C20(0.97)  LDD1568  [2]
 LDCM0345  AC8 HEK-293T C20(1.00)  LDD1569  [2]
 LDCM0248  AKOS034007472 HEK-293T C20(1.00)  LDD1511  [2]
 LDCM0275  AKOS034007705 HEK-293T C20(0.93)  LDD1514  [2]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [6]
 LDCM0367  CL1 HEK-293T C20(0.97)  LDD1571  [2]
 LDCM0368  CL10 HEK-293T C20(1.11)  LDD1572  [2]
 LDCM0369  CL100 HEK-293T C20(1.00)  LDD1573  [2]
 LDCM0370  CL101 HEK-293T C20(0.93)  LDD1574  [2]
 LDCM0371  CL102 HEK-293T C20(1.05)  LDD1575  [2]
 LDCM0372  CL103 HEK-293T C20(1.05)  LDD1576  [2]
 LDCM0373  CL104 HEK-293T C20(0.91)  LDD1577  [2]
 LDCM0374  CL105 HEK-293T C20(0.93)  LDD1578  [2]
 LDCM0375  CL106 HEK-293T C20(1.00)  LDD1579  [2]
 LDCM0376  CL107 HEK-293T C20(0.86)  LDD1580  [2]
 LDCM0377  CL108 HEK-293T C20(0.90)  LDD1581  [2]
 LDCM0378  CL109 HEK-293T C20(0.92)  LDD1582  [2]
 LDCM0379  CL11 HEK-293T C20(1.20)  LDD1583  [2]
 LDCM0380  CL110 HEK-293T C20(0.74)  LDD1584  [2]
 LDCM0381  CL111 HEK-293T C20(0.87)  LDD1585  [2]
 LDCM0382  CL112 HEK-293T C20(0.66)  LDD1586  [2]
 LDCM0383  CL113 HEK-293T C20(0.83)  LDD1587  [2]
 LDCM0384  CL114 HEK-293T C20(1.16)  LDD1588  [2]
 LDCM0385  CL115 HEK-293T C20(1.24)  LDD1589  [2]
 LDCM0386  CL116 HEK-293T C20(0.96)  LDD1590  [2]
 LDCM0387  CL117 HEK-293T C20(1.04)  LDD1591  [2]
 LDCM0388  CL118 HEK-293T C20(1.30)  LDD1592  [2]
 LDCM0389  CL119 HEK-293T C20(1.15)  LDD1593  [2]
 LDCM0390  CL12 HEK-293T C20(0.71)  LDD1594  [2]
 LDCM0391  CL120 HEK-293T C20(0.93)  LDD1595  [2]
 LDCM0392  CL121 HEK-293T C20(1.09)  LDD1596  [2]
 LDCM0393  CL122 HEK-293T C20(1.10)  LDD1597  [2]
 LDCM0394  CL123 HEK-293T C20(0.91)  LDD1598  [2]
 LDCM0395  CL124 HEK-293T C20(1.08)  LDD1599  [2]
 LDCM0396  CL125 HEK-293T C20(0.88)  LDD1600  [2]
 LDCM0397  CL126 HEK-293T C20(0.93)  LDD1601  [2]
 LDCM0398  CL127 HEK-293T C20(0.98)  LDD1602  [2]
 LDCM0399  CL128 HEK-293T C20(0.86)  LDD1603  [2]
 LDCM0400  CL13 HEK-293T C20(0.95)  LDD1604  [2]
 LDCM0401  CL14 HEK-293T C20(0.86)  LDD1605  [2]
 LDCM0402  CL15 HEK-293T C20(0.91)  LDD1606  [2]
 LDCM0403  CL16 HEK-293T C20(0.89)  LDD1607  [2]
 LDCM0405  CL18 HEK-293T C20(1.01)  LDD1609  [2]
 LDCM0406  CL19 HEK-293T C20(0.98)  LDD1610  [2]
 LDCM0407  CL2 HEK-293T C20(0.98)  LDD1611  [2]
 LDCM0408  CL20 HEK-293T C20(1.12)  LDD1612  [2]
 LDCM0409  CL21 HEK-293T C20(0.76)  LDD1613  [2]
 LDCM0410  CL22 HEK-293T C20(0.81)  LDD1614  [2]
 LDCM0411  CL23 HEK-293T C20(0.81)  LDD1615  [2]
 LDCM0412  CL24 HEK-293T C20(1.16)  LDD1616  [2]
 LDCM0413  CL25 HEK-293T C20(0.91)  LDD1617  [2]
 LDCM0414  CL26 HEK-293T C20(1.11)  LDD1618  [2]
 LDCM0415  CL27 HEK-293T C20(1.01)  LDD1619  [2]
 LDCM0416  CL28 HEK-293T C20(1.05)  LDD1620  [2]
 LDCM0418  CL3 HEK-293T C20(1.40)  LDD1622  [2]
 LDCM0419  CL30 HEK-293T C20(0.86)  LDD1623  [2]
 LDCM0420  CL31 HEK-293T C20(0.97)  LDD1624  [2]
 LDCM0421  CL32 HEK-293T C20(0.79)  LDD1625  [2]
 LDCM0422  CL33 HEK-293T C20(1.06)  LDD1626  [2]
 LDCM0423  CL34 HEK-293T C20(0.83)  LDD1627  [2]
 LDCM0424  CL35 HEK-293T C20(0.74)  LDD1628  [2]
 LDCM0425  CL36 HEK-293T C20(0.85)  LDD1629  [2]
 LDCM0426  CL37 HEK-293T C20(1.06)  LDD1630  [2]
 LDCM0428  CL39 HEK-293T C20(1.02)  LDD1632  [2]
 LDCM0429  CL4 HEK-293T C20(0.99)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C20(0.90)  LDD1634  [2]
 LDCM0432  CL42 HEK-293T C20(1.12)  LDD1636  [2]
 LDCM0433  CL43 HEK-293T C20(1.22)  LDD1637  [2]
 LDCM0434  CL44 HEK-293T C20(1.12)  LDD1638  [2]
 LDCM0435  CL45 HEK-293T C20(0.83)  LDD1639  [2]
 LDCM0436  CL46 HEK-293T C20(0.87)  LDD1640  [2]
 LDCM0437  CL47 HEK-293T C20(0.85)  LDD1641  [2]
 LDCM0438  CL48 HEK-293T C20(0.80)  LDD1642  [2]
 LDCM0439  CL49 HEK-293T C20(1.55)  LDD1643  [2]
 LDCM0441  CL50 HEK-293T C20(1.01)  LDD1645  [2]
 LDCM0443  CL52 HEK-293T C20(1.03)  LDD1646  [2]
 LDCM0445  CL54 HEK-293T C20(1.03)  LDD1648  [2]
 LDCM0446  CL55 HEK-293T C20(0.91)  LDD1649  [2]
 LDCM0447  CL56 HEK-293T C20(1.16)  LDD1650  [2]
 LDCM0448  CL57 HEK-293T C20(0.76)  LDD1651  [2]
 LDCM0449  CL58 HEK-293T C20(0.87)  LDD1652  [2]
 LDCM0450  CL59 HEK-293T C20(1.08)  LDD1653  [2]
 LDCM0451  CL6 HEK-293T C20(0.90)  LDD1654  [2]
 LDCM0452  CL60 HEK-293T C20(1.41)  LDD1655  [2]
 LDCM0453  CL61 HEK-293T C20(1.42)  LDD1656  [2]
 LDCM0454  CL62 HEK-293T C20(1.33)  LDD1657  [2]
 LDCM0455  CL63 HEK-293T C20(1.00)  LDD1658  [2]
 LDCM0456  CL64 HEK-293T C20(0.71)  LDD1659  [2]
 LDCM0458  CL66 HEK-293T C20(1.02)  LDD1661  [2]
 LDCM0459  CL67 HEK-293T C20(0.91)  LDD1662  [2]
 LDCM0460  CL68 HEK-293T C20(1.05)  LDD1663  [2]
 LDCM0461  CL69 HEK-293T C20(0.82)  LDD1664  [2]
 LDCM0462  CL7 HEK-293T C20(1.42)  LDD1665  [2]
 LDCM0463  CL70 HEK-293T C20(0.91)  LDD1666  [2]
 LDCM0464  CL71 HEK-293T C20(0.79)  LDD1667  [2]
 LDCM0465  CL72 HEK-293T C20(0.87)  LDD1668  [2]
 LDCM0466  CL73 HEK-293T C20(0.93)  LDD1669  [2]
 LDCM0467  CL74 HEK-293T C20(0.81)  LDD1670  [2]
 LDCM0469  CL76 HEK-293T C20(1.03)  LDD1672  [2]
 LDCM0471  CL78 HEK-293T C20(1.05)  LDD1674  [2]
 LDCM0472  CL79 HEK-293T C20(1.17)  LDD1675  [2]
 LDCM0473  CL8 HEK-293T C20(0.74)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C20(1.24)  LDD1677  [2]
 LDCM0475  CL81 HEK-293T C20(0.89)  LDD1678  [2]
 LDCM0476  CL82 HEK-293T C20(0.88)  LDD1679  [2]
 LDCM0477  CL83 HEK-293T C20(0.76)  LDD1680  [2]
 LDCM0478  CL84 HEK-293T C20(0.94)  LDD1681  [2]
 LDCM0479  CL85 HEK-293T C20(0.85)  LDD1682  [2]
 LDCM0480  CL86 HEK-293T C20(0.84)  LDD1683  [2]
 LDCM0481  CL87 HEK-293T C20(0.97)  LDD1684  [2]
 LDCM0482  CL88 HEK-293T C20(1.04)  LDD1685  [2]
 LDCM0484  CL9 HEK-293T C20(0.87)  LDD1687  [2]
 LDCM0485  CL90 HEK-293T C20(1.18)  LDD1688  [2]
 LDCM0486  CL91 HEK-293T C20(0.92)  LDD1689  [2]
 LDCM0487  CL92 HEK-293T C20(1.13)  LDD1690  [2]
 LDCM0488  CL93 HEK-293T C20(0.88)  LDD1691  [2]
 LDCM0489  CL94 HEK-293T C20(0.94)  LDD1692  [2]
 LDCM0490  CL95 HEK-293T C20(1.03)  LDD1693  [2]
 LDCM0491  CL96 HEK-293T C20(1.08)  LDD1694  [2]
 LDCM0492  CL97 HEK-293T C20(0.85)  LDD1695  [2]
 LDCM0493  CL98 HEK-293T C20(0.77)  LDD1696  [2]
 LDCM0494  CL99 HEK-293T C20(1.34)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C20(1.25)  LDD1698  [2]
 LDCM0468  Fragment33 HEK-293T C20(0.84)  LDD1671  [2]
 LDCM0427  Fragment51 HEK-293T C20(1.11)  LDD1631  [2]
 LDCM0107  IAA HeLa N.A.  LDD0221  [6]
 LDCM0109  NEM HeLa N.A.  LDD0223  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
COP9 signalosome complex subunit 3 (COPS3) CSN3 family Q9UNS2
Sulfite oxidase, mitochondrial (SUOX) . P51687
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Harmonin-binding protein USHBP1 (USHBP1) MCC family Q8N6Y0

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587