Details of the Target

General Information of Target

Target ID LDTP05667
Target Name Syntaxin-4 (STX4)
Gene Name STX4
Gene ID 6810
Synonyms
STX4A; Syntaxin-4; Renal carcinoma antigen NY-REN-31
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQ
ELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNT
RMRKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSG
QSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHDIFTFLATEVEMQGEMINR
IEKNILSSADYVERGQEHVKTALENQKKARKKKVLIAICVSITVVLLAVIIGVTVVG
Target Bioclass
Transporter and channel
Family
Syntaxin family
Subcellular location
Cell membrane
Function
Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. In neurons, recruited at neurite tips to membrane domains rich in the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) which promotes neurite tip surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.
Uniprot ID
Q12846
Ensemble ID
ENST00000313843.8
HGNC ID
HGNC:11439

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CHL1 SNV: p.G128E DBIA    Probe Info 
HEC1 SNV: p.I282V DBIA    Probe Info 
HEC1B SNV: p.I282V .
KELLY SNV: p.S14I .
MOLT4 SNV: p.V59I IA-alkyne    Probe Info 
SW1990 SNV: p.S15W DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		5.25  LDD0066  [1]
STPyne
 Probe Info  		K44(8.33)  LDD0277  [2]
IPM
 Probe Info  		N.A.  LDD0241  [3]
Probe 1
 Probe Info  		Y115(17.05)  LDD3495  [4]
DBIA
 Probe Info  		C141(1.34)  LDD3311  [5]
Acrolein
 Probe Info  		N.A.  LDD0223  [6]
ATP probe
 Probe Info  		N.A.  LDD0199  [7]
CY-1
 Probe Info  		E76(0.00); E77(0.00); K80(0.00)  LDD0246  [8]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [9]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [9]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [9]
TFBX
 Probe Info  		N.A.  LDD0027  [10]
NAIA_5
 Probe Info  		N.A.  LDD2223  [11]
PAL-AfBPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C220
 Probe Info  		11.79  LDD1894  [12]
C235
 Probe Info  		20.82  LDD1908  [12]
C243
 Probe Info  		10.63  LDD1916  [12]
C244
 Probe Info  		15.56  LDD1917  [12]
C245
 Probe Info  		5.39  LDD1918  [12]
C246
 Probe Info  		15.03  LDD1919  [12]
C249
 Probe Info  		12.38  LDD1922  [12]
VE-P
 Probe Info  		N.A.  LDD0396  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C141(1.01)  LDD0812  [14]
 LDCM0230  AC113 HEK-293T C141(0.96)  LDD0828  [14]
 LDCM0231  AC114 HEK-293T C141(0.92)  LDD0829  [14]
 LDCM0232  AC115 HEK-293T C141(1.00)  LDD0830  [14]
 LDCM0233  AC116 HEK-293T C141(1.00)  LDD0831  [14]
 LDCM0234  AC117 HEK-293T C141(1.22)  LDD0832  [14]
 LDCM0235  AC118 HEK-293T C141(1.01)  LDD0833  [14]
 LDCM0236  AC119 HEK-293T C141(0.95)  LDD0834  [14]
 LDCM0238  AC120 HEK-293T C141(1.09)  LDD0836  [14]
 LDCM0239  AC121 HEK-293T C141(1.13)  LDD0837  [14]
 LDCM0240  AC122 HEK-293T C141(1.05)  LDD0838  [14]
 LDCM0241  AC123 HEK-293T C141(1.14)  LDD0839  [14]
 LDCM0242  AC124 HEK-293T C141(0.96)  LDD0840  [14]
 LDCM0243  AC125 HEK-293T C141(1.12)  LDD0841  [14]
 LDCM0244  AC126 HEK-293T C141(1.07)  LDD0842  [14]
 LDCM0245  AC127 HEK-293T C141(1.15)  LDD0843  [14]
 LDCM0263  AC143 HEK-293T C141(0.84)  LDD0861  [14]
 LDCM0264  AC144 HEK-293T C141(1.05)  LDD0862  [14]
 LDCM0265  AC145 HEK-293T C141(1.04)  LDD0863  [14]
 LDCM0266  AC146 HEK-293T C141(0.96)  LDD0864  [14]
 LDCM0267  AC147 HEK-293T C141(1.17)  LDD0865  [14]
 LDCM0268  AC148 HEK-293T C141(1.10)  LDD0866  [14]
 LDCM0269  AC149 HEK-293T C141(1.16)  LDD0867  [14]
 LDCM0271  AC150 HEK-293T C141(1.13)  LDD0869  [14]
 LDCM0272  AC151 HEK-293T C141(0.99)  LDD0870  [14]
 LDCM0273  AC152 HEK-293T C141(1.04)  LDD0871  [14]
 LDCM0274  AC153 HEK-293T C141(1.14)  LDD0872  [14]
 LDCM0621  AC154 HEK-293T C141(0.97)  LDD2162  [14]
 LDCM0622  AC155 HEK-293T C141(0.96)  LDD2163  [14]
 LDCM0623  AC156 HEK-293T C141(1.15)  LDD2164  [14]
 LDCM0624  AC157 HEK-293T C141(1.42)  LDD2165  [14]
 LDCM0276  AC17 HEK-293T C141(0.93)  LDD0874  [14]
 LDCM0277  AC18 HEK-293T C141(0.96)  LDD0875  [14]
 LDCM0278  AC19 HEK-293T C141(1.00)  LDD0876  [14]
 LDCM0279  AC2 HEK-293T C141(1.11)  LDD0877  [14]
 LDCM0280  AC20 HEK-293T C141(0.86)  LDD0878  [14]
 LDCM0281  AC21 HEK-293T C141(0.87)  LDD0879  [14]
 LDCM0282  AC22 HEK-293T C141(0.89)  LDD0880  [14]
 LDCM0283  AC23 HEK-293T C141(0.84)  LDD0881  [14]
 LDCM0284  AC24 HEK-293T C141(0.88)  LDD0882  [14]
 LDCM0289  AC29 HEK-293T C141(1.04)  LDD1528  [15]
 LDCM0290  AC3 HEK-293T C141(1.07)  LDD0888  [14]
 LDCM0298  AC37 HEK-293T C141(1.18)  LDD1537  [15]
 LDCM0301  AC4 HEK-293T C141(1.23)  LDD0899  [14]
 LDCM0307  AC45 HEK-293T C141(1.09)  LDD1546  [15]
 LDCM0308  AC46 HEK-293T C141(1.06)  LDD0906  [14]
 LDCM0309  AC47 HEK-293T C141(1.04)  LDD0907  [14]
 LDCM0310  AC48 HEK-293T C141(0.99)  LDD0908  [14]
 LDCM0311  AC49 HEK-293T C141(0.87)  LDD0909  [14]
 LDCM0312  AC5 HEK-293T C141(1.00)  LDD0910  [14]
 LDCM0313  AC50 HEK-293T C141(0.91)  LDD0911  [14]
 LDCM0314  AC51 HEK-293T C141(0.75)  LDD0912  [14]
 LDCM0315  AC52 HEK-293T C141(0.76)  LDD0913  [14]
 LDCM0316  AC53 HEK-293T C141(0.84)  LDD0914  [14]
 LDCM0317  AC54 HEK-293T C141(0.93)  LDD0915  [14]
 LDCM0318  AC55 HEK-293T C141(0.97)  LDD0916  [14]
 LDCM0319  AC56 HEK-293T C141(1.01)  LDD0917  [14]
 LDCM0320  AC57 HEK-293T C141(0.96)  LDD0918  [14]
 LDCM0321  AC58 HEK-293T C141(0.87)  LDD0919  [14]
 LDCM0322  AC59 HEK-293T C141(0.85)  LDD0920  [14]
 LDCM0324  AC60 HEK-293T C141(0.62)  LDD0922  [14]
 LDCM0325  AC61 HEK-293T C141(0.92)  LDD0923  [14]
 LDCM0326  AC62 HEK-293T C141(0.96)  LDD0924  [14]
 LDCM0327  AC63 HEK-293T C141(0.84)  LDD0925  [14]
 LDCM0328  AC64 HEK-293T C141(0.88)  LDD0926  [14]
 LDCM0329  AC65 HEK-293T C141(0.88)  LDD0927  [14]
 LDCM0330  AC66 HEK-293T C141(0.92)  LDD0928  [14]
 LDCM0331  AC67 HEK-293T C141(0.83)  LDD0929  [14]
 LDCM0332  AC68 HEK-293T C141(1.09)  LDD0930  [14]
 LDCM0333  AC69 HEK-293T C141(0.99)  LDD0931  [14]
 LDCM0335  AC70 HEK-293T C141(1.09)  LDD0933  [14]
 LDCM0336  AC71 HEK-293T C141(1.06)  LDD0934  [14]
 LDCM0337  AC72 HEK-293T C141(1.14)  LDD0935  [14]
 LDCM0338  AC73 HEK-293T C141(1.20)  LDD0936  [14]
 LDCM0339  AC74 HEK-293T C141(1.19)  LDD0937  [14]
 LDCM0340  AC75 HEK-293T C141(1.22)  LDD0938  [14]
 LDCM0341  AC76 HEK-293T C141(1.08)  LDD0939  [14]
 LDCM0342  AC77 HEK-293T C141(1.33)  LDD0940  [14]
 LDCM0343  AC78 HEK-293T C141(1.07)  LDD0941  [14]
 LDCM0344  AC79 HEK-293T C141(1.13)  LDD0942  [14]
 LDCM0346  AC80 HEK-293T C141(1.06)  LDD0944  [14]
 LDCM0347  AC81 HEK-293T C141(1.19)  LDD0945  [14]
 LDCM0348  AC82 HEK-293T C141(1.20)  LDD0946  [14]
 LDCM0248  AKOS034007472 HEK-293T C141(1.14)  LDD1511  [15]
 LDCM0369  CL100 HEK-293T C141(1.07)  LDD0967  [14]
 LDCM0374  CL105 HEK-293T C141(0.80)  LDD0972  [14]
 LDCM0375  CL106 HEK-293T C141(0.95)  LDD0973  [14]
 LDCM0376  CL107 HEK-293T C141(0.98)  LDD0974  [14]
 LDCM0377  CL108 HEK-293T C141(0.98)  LDD0975  [14]
 LDCM0378  CL109 HEK-293T C141(0.91)  LDD0976  [14]
 LDCM0380  CL110 HEK-293T C141(0.91)  LDD0978  [14]
 LDCM0381  CL111 HEK-293T C141(0.79)  LDD0979  [14]
 LDCM0392  CL121 HEK-293T C141(0.99)  LDD0990  [14]
 LDCM0393  CL122 HEK-293T C141(0.95)  LDD0991  [14]
 LDCM0394  CL123 HEK-293T C141(0.92)  LDD0992  [14]
 LDCM0395  CL124 HEK-293T C141(1.09)  LDD0993  [14]
 LDCM0396  CL125 HEK-293T C141(0.86)  LDD0994  [14]
 LDCM0397  CL126 HEK-293T C141(0.85)  LDD0995  [14]
 LDCM0398  CL127 HEK-293T C141(0.85)  LDD0996  [14]
 LDCM0399  CL128 HEK-293T C141(1.05)  LDD0997  [14]
 LDCM0409  CL21 HEK-293T C141(1.01)  LDD1613  [15]
 LDCM0422  CL33 HEK-293T C141(1.07)  LDD1626  [15]
 LDCM0435  CL45 HEK-293T C141(1.00)  LDD1639  [15]
 LDCM0448  CL57 HEK-293T C141(1.13)  LDD1651  [15]
 LDCM0453  CL61 HEK-293T C141(1.15)  LDD1051  [14]
 LDCM0454  CL62 HEK-293T C141(0.96)  LDD1052  [14]
 LDCM0455  CL63 HEK-293T C141(0.79)  LDD1053  [14]
 LDCM0456  CL64 HEK-293T C141(0.78)  LDD1054  [14]
 LDCM0457  CL65 HEK-293T C141(0.82)  LDD1055  [14]
 LDCM0458  CL66 HEK-293T C141(0.80)  LDD1056  [14]
 LDCM0459  CL67 HEK-293T C141(0.97)  LDD1057  [14]
 LDCM0460  CL68 HEK-293T C141(0.95)  LDD1058  [14]
 LDCM0461  CL69 HEK-293T C141(0.95)  LDD1059  [14]
 LDCM0463  CL70 HEK-293T C141(0.97)  LDD1061  [14]
 LDCM0464  CL71 HEK-293T C141(0.95)  LDD1062  [14]
 LDCM0465  CL72 HEK-293T C141(0.86)  LDD1063  [14]
 LDCM0466  CL73 HEK-293T C141(0.93)  LDD1064  [14]
 LDCM0467  CL74 HEK-293T C141(0.97)  LDD1065  [14]
 LDCM0475  CL81 HEK-293T C141(1.48)  LDD1678  [15]
 LDCM0484  CL9 HEK-293T C141(1.12)  LDD1687  [15]
 LDCM0486  CL91 HEK-293T C141(0.88)  LDD1084  [14]
 LDCM0487  CL92 HEK-293T C141(0.93)  LDD1085  [14]
 LDCM0488  CL93 HEK-293T C141(0.90)  LDD1086  [14]
 LDCM0489  CL94 HEK-293T C141(0.89)  LDD1087  [14]
 LDCM0490  CL95 HEK-293T C141(0.91)  LDD1088  [14]
 LDCM0491  CL96 HEK-293T C141(0.98)  LDD1089  [14]
 LDCM0492  CL97 HEK-293T C141(0.96)  LDD1090  [14]
 LDCM0493  CL98 HEK-293T C141(0.95)  LDD1091  [14]
 LDCM0494  CL99 HEK-293T C141(1.03)  LDD1092  [14]
 LDCM0468  Fragment33 HEK-293T C141(0.67)  LDD1066  [14]
 LDCM0022  KB02 HEK-293T C141(0.98)  LDD1492  [15]
 LDCM0023  KB03 HEK-293T C141(1.09)  LDD1497  [15]
 LDCM0024  KB05 G361 C141(1.34)  LDD3311  [5]
 LDCM0109  NEM HeLa N.A.  LDD0223  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Sphingosine-1-phosphate lyase 1 (SGPL1) Group II decarboxylase family O95470
Proteasome subunit alpha type-3 (PSMA3) Peptidase T1A family P25788
Fatty acid hydroxylase domain-containing protein 2 (FAXDC2) Sterol desaturase family Q96IV6
GTPase IMAP family member 5 (GIMAP5) AIG1/Toc34/Toc159-like paraseptin GTPase family Q96F15
E3 ubiquitin-protein ligase RNF4 (RNF4) . P78317
Plasma membrane ascorbate-dependent reductase CYBRD1 (CYBRD1) . Q53TN4
Transporter and channel
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Vesicle-associated membrane protein 1 (VAMP1) Synaptobrevin family P23763
Vesicle-associated membrane protein 2 (VAMP2) Synaptobrevin family P63027
Vesicle-associated membrane protein 3 (VAMP3) Synaptobrevin family Q15836
Syntaxin-1A (STX1A) Syntaxin family Q16623
Syntaxin-3 (STX3) Syntaxin family Q13277
Syntaxin-5 (STX5) Syntaxin family Q13190
Syntaxin-6 (STX6) Syntaxin family O43752
Vesicle transport through interaction with t-SNAREs homolog 1B (VTI1B) VTI1 family Q9UEU0
Transmembrane protein 199 (TMEM199) . Q8N511
Transmembrane protein 203 (TMEM203) . Q969S6
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Probable G-protein coupled receptor 152 (GPR152) G-protein coupled receptor 1 family Q8TDT2
Other
Click To Hide/Show 20 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
BET1 homolog (BET1) BET1 family O15155
Golgi SNAP receptor complex member 2 (GOSR2) GOSR2 family O14653
Stress-associated endoplasmic reticulum protein 1 (SERP1) RAMP4 family Q9Y6X1
Alpha-soluble NSF attachment protein (NAPA) SNAP family P54920
Beta-soluble NSF attachment protein (NAPB) SNAP family Q9H115
Synaptosomal-associated protein 47 (SNAP47) SVAP1 family Q5SQN1
Vesicle-associated membrane protein 4 (VAMP4) Synaptobrevin family O75379
Vesicle-associated membrane protein 5 (VAMP5) Synaptobrevin family O95183
Vesicle-trafficking protein SEC22a (SEC22A) Synaptobrevin family Q96IW7
Vesicle-trafficking protein SEC22b (SEC22B) Synaptobrevin family O75396
Syntaxin-11 (STX11) Syntaxin family O75558
Syntaxin-12 (STX12) Syntaxin family Q86Y82
Syntaxin-16 (STX16) Syntaxin family O14662
Syntaxin-1B (STX1B) Syntaxin family P61266
Syntaxin-7 (STX7) Syntaxin family O15400
Syntaxin-8 (STX8) Syntaxin family Q9UNK0
Vesicle transport protein USE1 (USE1) USE1 family Q9NZ43
Zinc finger protein-like 1 (ZFPL1) ZFPL1 family O95159
Emerin (EMD) . P50402
TRAF3-interacting JNK-activating modulator (TRAF3IP3) . Q9Y228

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
8 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
11 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
12 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
13 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
14 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
15 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402