General Information of Target

Target ID LDTP05418
Target Name UBX domain-containing protein 1 (UBXN1)
Gene Name UBXN1
Gene ID 51035
Synonyms
SAKS1; UBX domain-containing protein 1; SAPK substrate protein 1; UBA/UBX 33.3 kDa protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAELTALESLIEMGFPRGRAEKALALTGNQGIEAAMDWLMEHEDDPDVDEPLETPLGHIL
GREPTSSEQGGLEGSGSAAGEGKPALSEEERQEQTKRMLELVAQKQREREEREEREALER
ERQRRRQGQELSAARQRLQEDEMRRAAEERRREKAEELAARQRVREKIERDKAERAKKYG
GSVGSQPPPVAPEPGPVPSSPSQEPPTKREYDQCRIQVRLPDGTSLTQTFRAREQLAAVR
LYVELHRGEELGGGQDPVQLLSGFPRRAFSEADMERPLQELGLVPSAVLIVAKKCPS
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Ubiquitin-binding protein that plays a role in the modulation of innate immune response. Blocks both the RIG-I-like receptors (RLR) and NF-kappa-B pathways. Following viral infection, UBXN1 is induced and recruited to the RLR component MAVS. In turn, interferes with MAVS oligomerization, and disrupts the MAVS/TRAF3/TRAF6 signalosome. This function probably serves as a brake to prevent excessive RLR signaling. Interferes with the TNFalpha-triggered NF-kappa-B pathway by interacting with cellular inhibitors of apoptosis proteins (cIAPs) and thereby inhibiting their recruitment to TNFR1. Prevents also the activation of NF-kappa-B by associating with CUL1 and thus inhibiting NF-kappa-B inhibitor alpha/NFKBIA degradation that remains bound to NF-kappa-B. Interacts with the BRCA1-BARD1 heterodimer and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1 leads to the inhibition of the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol.
Uniprot ID
Q04323
Ensemble ID
ENST00000294119.6
HGNC ID
HGNC:18402

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL78 SNV: p.E3D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 19 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info 
15.00  LDD0402  [1]
ONAyne
 Probe Info 
K154(6.25)  LDD0274  [2]
STPyne
 Probe Info 
K105(10.00)  LDD0277  [2]
DBIA
 Probe Info 
C214(1.48)  LDD3410  [3]
BTD
 Probe Info 
C214(1.00)  LDD2107  [4]
DA-P3
 Probe Info 
6.27  LDD0180  [5]
HHS-475
 Probe Info 
Y179(13.74)  LDD0268  [6]
5E-2FA
 Probe Info 
N.A.  LDD2235  [7]
ATP probe
 Probe Info 
N.A.  LDD0199  [8]
IA-alkyne
 Probe Info 
N.A.  LDD0162  [9]
IPIAA_L
 Probe Info 
N.A.  LDD0031  [10]
IPM
 Probe Info 
N.A.  LDD0147  [11]
SF
 Probe Info 
Y242(0.00); Y179(0.00)  LDD0028  [12]
TFBX
 Probe Info 
N.A.  LDD0148  [11]
Phosphinate-6
 Probe Info 
N.A.  LDD0018  [13]
Acrolein
 Probe Info 
N.A.  LDD0217  [14]
Crotonaldehyde
 Probe Info 
N.A.  LDD0219  [14]
Methacrolein
 Probe Info 
N.A.  LDD0218  [14]
AOyne
 Probe Info 
13.10  LDD0443  [15]
PAL-AfBPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C201
 Probe Info 
25.63  LDD1877  [16]
C244
 Probe Info 
13.83  LDD1917  [16]
C284
 Probe Info 
20.97  LDD1954  [16]
FFF probe11
 Probe Info 
20.00  LDD0471  [17]
FFF probe2
 Probe Info 
20.00  LDD0463  [17]
FFF probe3
 Probe Info 
20.00  LDD0464  [17]
DA-2
 Probe Info 
N.A.  LDD0070  [18]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C214(1.12)  LDD1509  [19]
 LDCM0270  AC15 HEK-293T C214(0.71)  LDD1513  [19]
 LDCM0278  AC19 HEK-293T C214(0.79)  LDD1517  [19]
 LDCM0283  AC23 HEK-293T C214(0.90)  LDD1522  [19]
 LDCM0287  AC27 HEK-293T C214(0.87)  LDD1526  [19]
 LDCM0290  AC3 HEK-293T C214(1.00)  LDD1529  [19]
 LDCM0292  AC31 HEK-293T C214(0.75)  LDD1531  [19]
 LDCM0296  AC35 HEK-293T C214(0.85)  LDD1535  [19]
 LDCM0300  AC39 HEK-293T C214(0.81)  LDD1539  [19]
 LDCM0305  AC43 HEK-293T C214(0.88)  LDD1544  [19]
 LDCM0309  AC47 HEK-293T C214(1.08)  LDD1548  [19]
 LDCM0314  AC51 HEK-293T C214(1.15)  LDD1553  [19]
 LDCM0318  AC55 HEK-293T C214(0.79)  LDD1557  [19]
 LDCM0322  AC59 HEK-293T C214(1.13)  LDD1561  [19]
 LDCM0327  AC63 HEK-293T C214(0.73)  LDD1566  [19]
 LDCM0334  AC7 HEK-293T C214(0.99)  LDD1568  [19]
 LDCM0156  Aniline NCI-H1299 11.93  LDD0403  [1]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [14]
 LDCM0379  CL11 HEK-293T C214(1.26)  LDD1583  [19]
 LDCM0406  CL19 HEK-293T C214(1.07)  LDD1610  [19]
 LDCM0411  CL23 HEK-293T C214(1.23)  LDD1615  [19]
 LDCM0420  CL31 HEK-293T C214(0.99)  LDD1624  [19]
 LDCM0424  CL35 HEK-293T C214(1.79)  LDD1628  [19]
 LDCM0433  CL43 HEK-293T C214(1.44)  LDD1637  [19]
 LDCM0437  CL47 HEK-293T C214(1.78)  LDD1641  [19]
 LDCM0446  CL55 HEK-293T C214(1.22)  LDD1649  [19]
 LDCM0450  CL59 HEK-293T C214(1.96)  LDD1653  [19]
 LDCM0459  CL67 HEK-293T C214(1.05)  LDD1662  [19]
 LDCM0462  CL7 HEK-293T C214(0.93)  LDD1665  [19]
 LDCM0464  CL71 HEK-293T C214(1.40)  LDD1667  [19]
 LDCM0472  CL79 HEK-293T C214(0.95)  LDD1675  [19]
 LDCM0477  CL83 HEK-293T C214(1.47)  LDD1680  [19]
 LDCM0486  CL91 HEK-293T C214(1.09)  LDD1689  [19]
 LDCM0490  CL95 HEK-293T C214(1.14)  LDD1693  [19]
 LDCM0028  Dobutamine HEK-293T 6.27  LDD0180  [5]
 LDCM0120  HHS-0701 DM93 Y179(13.74)  LDD0268  [6]
 LDCM0107  IAA HeLa N.A.  LDD0221  [14]
 LDCM0022  KB02 A431 C214(1.55)  LDD2258  [3]
 LDCM0023  KB03 A431 C214(1.34)  LDD2675  [3]
 LDCM0024  KB05 RPMI-7951 C214(1.48)  LDD3410  [3]
 LDCM0030  Luteolin HEK-293T 11.44  LDD0182  [5]
 LDCM0109  NEM HeLa N.A.  LDD0233  [14]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C214(1.00)  LDD2107  [4]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C214(0.58)  LDD2133  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Peroxisomal bifunctional enzyme (EHHADH) Enoyl-CoA hydratase/isomerase family; 3-hydroxyacyl-CoA dehydrogenase family Q08426
DNA-directed RNA polymerase III subunit RPC3 (POLR3C) Eukaryotic RPC3/POLR3C RNA polymerase subunit family Q9BUI4
E3 ubiquitin-protein ligase TRIM39 (TRIM39) TRIM/RBCC family Q9HCM9
Phospholipase A-2-activating protein (PLAA) WD repeat PLAP family Q9Y263
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-synuclein (SNCA) Synuclein family P37840
Wolframin (WFS1) . O76024
Other
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Prelamin-A/C (LMNA) Intermediate filament family P02545
UV excision repair protein RAD23 homolog A (RAD23A) RAD23 family P54725
Heat shock protein beta-1 (HSPB1) Small heat shock protein (HSP20) family P04792
Heat shock protein beta-7 (HSPB7) Small heat shock protein (HSP20) family Q9UBY9
RING finger protein 11 (RNF11) . Q9Y3C5
Ubiquilin-1 (UBQLN1) . Q9UMX0
Ubiquilin-2 (UBQLN2) . Q9UHD9

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
6 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
9 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
10 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
11 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
12 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
13 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
14 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
15 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
16 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
17 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
18 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
19 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402