Details of the Probe

General Information of Probe

Probe ID
LDPC0280
Probe Name
C011
Synonyms
Z4286591438
Probe Type
PAL-AfBPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 320.4
Lipid-water partition coefficient (xlogp) 2.9
Rotatable Bond Count (rotbonds) 7
Hydrogen Bond Donor Count (hbonddonor) 1
Hydrogen Bond Acceptor Count (hbondacc) 4
Chemical Identifiers
Formula
C19H20N4O
IUPAC Name
N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]-1-(2-cyanophenyl)cyclobutane-1-carboxamide
Canonical SMILES
C#CCCC1(N=N1)CCNC(=O)C2(CCC2)C3=CC=CC=C3C#N
InChI
InChI=1S/C19H20N4O/c1-2-3-11-19(22-23-19)12-13-21-17(24)18(9-6-10-18)16-8-5-4-7-15(16)14-20/h1,4-5,7-8H,3,6,9-13H2,(H,21,24)
InChIKey
SLYBQSDUOKDVCX-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

80 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Bis(monoacylglycero)phosphate synthase CLN5 (CLN5) LDTP01241 6.73  LDD1720 
Bis(monoacylglycero)phosphate synthase CLN5 (CLN5) LDTP01241 6.73  LDD1720 
CAAX prenyl protease 1 homolog (ZMPSTE24) LDTP01328 5.10  LDD1720 
CAAX prenyl protease 1 homolog (ZMPSTE24) LDTP01328 5.10  LDD1720 
Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) LDTP03257 5.62  LDD1720 
Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) LDTP03257 5.62  LDD1720 
Ceramide synthase 2 (CERS2) LDTP10329 7.89  LDD1720 
Ceramide synthase 2 (CERS2) LDTP10329 7.89  LDD1720 
Cytochrome b5 type B (CYB5B) LDTP00697 5.17  LDD1720 
Cytochrome b5 type B (CYB5B) LDTP00697 5.17  LDD1720 
Delta(24)-sterol reductase (DHCR24) LDTP06375 7.73  LDD1720 
Delta(24)-sterol reductase (DHCR24) LDTP06375 7.73  LDD1720 
Dihydrofolate reductase (DHFR) LDTP01769 13.83  LDD1720 
Dihydrofolate reductase (DHFR) LDTP01769 13.83  LDD1720 
E3 ubiquitin-protein ligase RAD18 (RAD18) LDTP12573 5.54  LDD1720 
E3 ubiquitin-protein ligase RAD18 (RAD18) LDTP12573 5.54  LDD1720 
Endoplasmic reticulum aminopeptidase 1 (ERAP1) LDTP12868 5.17  LDD1720 
Endoplasmic reticulum aminopeptidase 1 (ERAP1) LDTP12868 5.17  LDD1720 
Endoplasmic reticulum transmembrane helix translocase (ATP13A1) LDTP12279 4.92  LDD1720 
Endoplasmic reticulum transmembrane helix translocase (ATP13A1) LDTP12279 4.92  LDD1720 
Endothelin-converting enzyme 1 (ECE1) LDTP04008 8.51  LDD1720 
Endothelin-converting enzyme 1 (ECE1) LDTP04008 8.51  LDD1720 
FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) LDTP10188 7.41  LDD1720 
FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) LDTP10188 7.41  LDD1720 
Glycylpeptide N-tetradecanoyltransferase 1 (NMT1) LDTP03537 5.62  LDD1720 
Glycylpeptide N-tetradecanoyltransferase 1 (NMT1) LDTP03537 5.62  LDD1720 
Glyoxalase domain-containing protein 4 (GLOD4) LDTP16040 5.70  LDD1720 
Glyoxalase domain-containing protein 4 (GLOD4) LDTP16040 5.70  LDD1720 
Heme oxygenase 1 (HMOX1) LDTP02359 5.43  LDD1720 
Heme oxygenase 1 (HMOX1) LDTP02359 5.43  LDD1720 
Heme oxygenase 2 (HMOX2) LDTP03540 5.54  LDD1720 
Heme oxygenase 2 (HMOX2) LDTP03540 5.54  LDD1720 
Lysophospholipid acyltransferase 7 (MBOAT7) LDTP10612 5.06  LDD1720 
Lysophospholipid acyltransferase 7 (MBOAT7) LDTP10612 5.06  LDD1720 
Lysosomal acid glucosylceramidase (GBA1) LDTP02005 5.13  LDD1720 
Lysosomal acid glucosylceramidase (GBA1) LDTP02005 5.13  LDD1720 
Lysosomal Pro-X carboxypeptidase (PRCP) LDTP04004 9.06  LDD1720 
Lysosomal Pro-X carboxypeptidase (PRCP) LDTP04004 9.06  LDD1720 
Lysosomal protective protein (CTSA) LDTP02505 8.94  LDD1720 
Lysosomal protective protein (CTSA) LDTP02505 8.94  LDD1720 
Methylsterol monooxygenase 1 (MSMO1) LDTP06477 5.94  LDD1720 
Methylsterol monooxygenase 1 (MSMO1) LDTP06477 5.94  LDD1720 
Microsomal glutathione S-transferase 2 (MGST2) LDTP10966 6.28  LDD1720 
Microsomal glutathione S-transferase 2 (MGST2) LDTP10966 6.28  LDD1720 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 9.71  LDD1720 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 9.71  LDD1720 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 7.11  LDD1720 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 7.11  LDD1720 
Peptidyl-prolyl cis-trans isomerase FKBP1A (FKBP1A) LDTP05029 5.24  LDD1720 
Peptidyl-prolyl cis-trans isomerase FKBP1A (FKBP1A) LDTP05029 5.24  LDD1720 
Peroxisomal ATPase PEX1 (PEX1) LDTP00910 5.17  LDD1720 
Peroxisomal ATPase PEX1 (PEX1) LDTP00910 5.17  LDD1720 
Phospholipase A2 group XV (PLA2G15) LDTP09088 12.64  LDD1720 
Phospholipase A2 group XV (PLA2G15) LDTP09088 12.64  LDD1720 
Prenylcysteine oxidase 1 (PCYOX1) LDTP13282 9.51  LDD1720 
Prenylcysteine oxidase 1 (PCYOX1) LDTP13282 9.51  LDD1720 
Probable serine carboxypeptidase CPVL (CPVL) LDTP15988 8.75  LDD1720 
Probable serine carboxypeptidase CPVL (CPVL) LDTP15988 8.75  LDD1720 
Proteasome subunit beta type-2 (PSMB2) LDTP04285 4.99  LDD1720 
Proteasome subunit beta type-2 (PSMB2) LDTP04285 4.99  LDD1720 
Protein O-GlcNAcase (OGA) LDTP00988 5.58  LDD1720 
Protein O-GlcNAcase (OGA) LDTP00988 5.58  LDD1720 
Protein-S-isoprenylcysteine O-methyltransferase (ICMT) LDTP01039 6.19  LDD1720 
Protein-S-isoprenylcysteine O-methyltransferase (ICMT) LDTP01039 6.19  LDD1720 
Putative phospholipase B-like 2 (PLBD2) LDTP09256 19.29  LDD1720 
Putative phospholipase B-like 2 (PLBD2) LDTP09256 19.29  LDD1720 
Retinoid-inducible serine carboxypeptidase (SCPEP1) LDTP16032 8.22  LDD1720 
Retinoid-inducible serine carboxypeptidase (SCPEP1) LDTP16032 8.22  LDD1720 
S-methyl-5'-thioadenosine phosphorylase (MTAP) LDTP05758 15.35  LDD1720 
S-methyl-5'-thioadenosine phosphorylase (MTAP) LDTP05758 15.35  LDD1720 
Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) LDTP15484 6.23  LDD1720 
Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) LDTP15484 6.23  LDD1720 
Serum paraoxonase/arylesterase 2 (PON2) LDTP06333 11.96  LDD1720 
Serum paraoxonase/arylesterase 2 (PON2) LDTP06333 11.96  LDD1720 
Signal peptidase complex catalytic subunit SEC11A (SEC11A) LDTP05066 5.03  LDD1720 
Signal peptidase complex catalytic subunit SEC11A (SEC11A) LDTP05066 5.03  LDD1720 
Tripeptidyl-peptidase 1 (TPP1) LDTP00428 7.11  LDD1720 
Tripeptidyl-peptidase 1 (TPP1) LDTP00428 7.11  LDD1720 
Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) LDTP08728 5.21  LDD1720 
Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) LDTP08728 5.21  LDD1720 
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⏷ Show the Full List of 80 Enzyme
58 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
ATP synthase subunit g, mitochondrial (ATP5MG) LDTP14511 7.89  LDD1720 
ATP synthase subunit g, mitochondrial (ATP5MG) LDTP14511 7.89  LDD1720 
Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) LDTP02724 5.74  LDD1720 
Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) LDTP02724 5.74  LDD1720 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 27.86  LDD1720 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 27.86  LDD1720 
Lysosome membrane protein 2 (SCARB2) LDTP06034 8.63  LDD1720 
Lysosome membrane protein 2 (SCARB2) LDTP06034 8.63  LDD1720 
MFS-type transporter SLC18B1 (SLC18B1) LDTP07462 6.11  LDD1720 
MFS-type transporter SLC18B1 (SLC18B1) LDTP07462 6.11  LDD1720 
Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) LDTP10913 19.43  LDD1720 
Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) LDTP10913 19.43  LDD1720 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 27.67  LDD1720 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 27.67  LDD1720 
Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) LDTP07885 16.11  LDD1720 
Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) LDTP07885 16.11  LDD1720 
Mpv17-like protein 2 (MPV17L2) LDTP14952 5.78  LDD1720 
Mpv17-like protein 2 (MPV17L2) LDTP14952 5.78  LDD1720 
NPC intracellular cholesterol transporter 1 (NPC1) LDTP00541 5.66  LDD1720 
NPC intracellular cholesterol transporter 1 (NPC1) LDTP00541 5.66  LDD1720 
Peroxisomal membrane protein 2 (PXMP2) LDTP16069 11.47  LDD1720 
Peroxisomal membrane protein 2 (PXMP2) LDTP16069 11.47  LDD1720 
Protein GPR107 (GPR107) LDTP07236 7.01  LDD1720 
Protein GPR107 (GPR107) LDTP07236 7.01  LDD1720 
Protein GPR108 (GPR108) LDTP12383 5.46  LDD1720 
Protein GPR108 (GPR108) LDTP12383 5.46  LDD1720 
Protein tweety homolog 3 (TTYH3) LDTP11615 5.82  LDD1720 
Protein tweety homolog 3 (TTYH3) LDTP11615 5.82  LDD1720 
Scavenger receptor class B member 1 (SCARB1) LDTP09547 8.17  LDD1720 
Scavenger receptor class B member 1 (SCARB1) LDTP09547 8.17  LDD1720 
Sigma intracellular receptor 2 (TMEM97) LDTP06945 10.20  LDD1720 
Sigma intracellular receptor 2 (TMEM97) LDTP06945 10.20  LDD1720 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 7.62  LDD1720 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 7.62  LDD1720 
Solute carrier family 35 member F2 (SLC35F2) LDTP17641 5.10  LDD1720 
Solute carrier family 35 member F2 (SLC35F2) LDTP17641 5.10  LDD1720 
StAR-related lipid transfer protein 3 (STARD3) LDTP06221 6.11  LDD1720 
StAR-related lipid transfer protein 3 (STARD3) LDTP06221 6.11  LDD1720 
StAR-related lipid transfer protein 7, mitochondrial (STARD7) LDTP18459 10.93  LDD1720 
StAR-related lipid transfer protein 7, mitochondrial (STARD7) LDTP18459 10.93  LDD1720 
Surfeit locus protein 4 (SURF4) LDTP00592 11.39  LDD1720 
Surfeit locus protein 4 (SURF4) LDTP00592 11.39  LDD1720 
Tetraspanin-3 (TSPAN3) LDTP16813 6.45  LDD1720 
Tetraspanin-3 (TSPAN3) LDTP16813 6.45  LDD1720 
Translocating chain-associated membrane protein 1 (TRAM1) LDTP06426 11.39  LDD1720 
Translocating chain-associated membrane protein 1 (TRAM1) LDTP06426 11.39  LDD1720 
Translocator protein (TSPO) LDTP03546 7.06  LDD1720 
Translocator protein (TSPO) LDTP03546 7.06  LDD1720 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 6.36  LDD1720 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 6.36  LDD1720 
Transmembrane 9 superfamily member 3 (TM9SF3) LDTP18448 5.06  LDD1720 
Transmembrane 9 superfamily member 3 (TM9SF3) LDTP18448 5.06  LDD1720 
Transmembrane protein 199 (TMEM199) LDTP08881 6.19  LDD1720 
Transmembrane protein 199 (TMEM199) LDTP08881 6.19  LDD1720 
Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 14.72  LDD1720 
Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 14.72  LDD1720 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 13.27  LDD1720 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 13.27  LDD1720 
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⏷ Show the Full List of 58 Transporter and channel
2 Transcription factor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Forkhead-associated domain-containing protein 1 (FHAD1) LDTP16717 9.78  LDD1720 
Forkhead-associated domain-containing protein 1 (FHAD1) LDTP16717 9.78  LDD1720 
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2 Immunoglobulin Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
IgG receptor FcRn large subunit p51 (FCGRT) LDTP04720 12.91  LDD1720 
IgG receptor FcRn large subunit p51 (FCGRT) LDTP04720 12.91  LDD1720 
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32 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Apolipoprotein L2 (APOL2) LDTP11076 14.22  LDD1720 
Apolipoprotein L2 (APOL2) LDTP11076 14.22  LDD1720 
Clustered mitochondria protein homolog (CLUH) LDTP14486 6.06  LDD1720 
Clustered mitochondria protein homolog (CLUH) LDTP14486 6.06  LDD1720 
Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) LDTP13902 5.78  LDD1720 
Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) LDTP13902 5.78  LDD1720 
EF-hand calcium-binding domain-containing protein 14 (EFCAB14) LDTP19021 6.82  LDD1720 
EF-hand calcium-binding domain-containing protein 14 (EFCAB14) LDTP19021 6.82  LDD1720 
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) LDTP13828 5.78  LDD1720 
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) LDTP13828 5.78  LDD1720 
ER lumen protein-retaining receptor 2 (KDELR2) LDTP03678 27.28  LDD1720 
ER lumen protein-retaining receptor 2 (KDELR2) LDTP03678 27.28  LDD1720 
Galectin-3-binding protein (LGALS3BP) LDTP05558 5.13  LDD1720 
Galectin-3-binding protein (LGALS3BP) LDTP05558 5.13  LDD1720 
Golgi integral membrane protein 4 (GOLIM4) LDTP00281 6.45  LDD1720 
Golgi integral membrane protein 4 (GOLIM4) LDTP00281 6.45  LDD1720 
Mammalian ependymin-related protein 1 (EPDR1) LDTP13606 15.14  LDD1720 
Mammalian ependymin-related protein 1 (EPDR1) LDTP13606 15.14  LDD1720 
Mitochondrial fission process protein 1 (MTFP1) LDTP16192 24.76  LDD1720 
Mitochondrial fission process protein 1 (MTFP1) LDTP16192 24.76  LDD1720 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 14.72  LDD1720 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 14.72  LDD1720 
Nucleobindin-1 (NUCB1) LDTP05375 5.58  LDD1720 
Nucleobindin-1 (NUCB1) LDTP05375 5.58  LDD1720 
Nucleobindin-2 (NUCB2) LDTP05160 9.00  LDD1720 
Nucleobindin-2 (NUCB2) LDTP05160 9.00  LDD1720 
Protein unc-119 homolog B (UNC119B) LDTP00105 6.63  LDD1720 
Protein unc-119 homolog B (UNC119B) LDTP00105 6.63  LDD1720 
TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L (TAF6L) LDTP14263 5.31  LDD1720 
TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L (TAF6L) LDTP14263 5.31  LDD1720 
Tetraspanin-6 (TSPAN6) LDTP14458 6.23  LDD1720 
Tetraspanin-6 (TSPAN6) LDTP14458 6.23  LDD1720 
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⏷ Show the Full List of 32 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD1720 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587