Details of the Target

General Information of Target

Target ID LDTP10966
Target Name Microsomal glutathione S-transferase 2 (MGST2)
Gene Name MGST2
Gene ID 4258
Synonyms
GST2; Microsomal glutathione S-transferase 2; Microsomal GST-2; EC 2.5.1.18; Glutathione peroxidase MGST2; EC 1.11.1.-; Leukotriene C4 synthase MGST2; EC 4.4.1.20; Microsomal glutathione S-transferase II; Microsomal GST-II
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MADHSFSDGVPSDSVEAAKNASNTEKLTDQVMQNPRVLAALQERLDNVPHTPSSYIETLP
KAVKRRINALKQLQVRCAHIEAKFYEEVHDLERKYAALYQPLFDKRREFITGDVEPTDAE
SEWHSENEEEEKLAGDMKSKVVVTEKAAATAEEPDPKGIPEFWFTIFRNVDMLSELVQEY
DEPILKHLQDIKVKFSDPGQPMSFVLEFHFEPNDYFTNSVLTKTYKMKSEPDKADPFSFE
GPEIVDCDGCTIDWKKGKNVTVKTIKKKQKHKGRGTVRTITKQVPNESFFNFFNPLKASG
DGESLDEDSEFTLASDFEIGHFFRERIVPRAVLYFTGEAIEDDDNFEEGEEGEEEELEGD
EEGEDEDDAEINPKV
Target Bioclass
Enzyme
Family
MAPEG family
Subcellular location
Endoplasmic reticulum membrane
Function
Catalyzes several different glutathione-dependent reactions. Catalyzes the glutathione-dependent reduction of lipid hydroperoxides, such as 5-HPETE. Has glutathione transferase activity, toward xenobiotic electrophiles, such as 1-chloro-2, 4-dinitrobenzene (CDNB). Catalyzes also the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4 (LTC4). Involved in oxidative DNA damage induced by ER stress and anticancer agents by activating LTC4 biosynthetic machinery in nonimmune cells.
Uniprot ID
Q99735
Ensemble ID
ENST00000265498.6
HGNC ID
HGNC:7063
ChEMBL ID
CHEMBL1743185

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
SW620 SNV: p.Q54Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HDSF-alk
 Probe Info  		1.74  LDD0197  [1]
YN-1
 Probe Info  		100.00  LDD0444  [2]
NAIA_5
 Probe Info  		N.A.  LDD2223  [3]
PAL-AfBPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C011
 Probe Info  		6.28  LDD1720  [4]
C056
 Probe Info  		17.75  LDD1753  [4]
C091
 Probe Info  		14.93  LDD1782  [4]
C092
 Probe Info  		23.75  LDD1783  [4]
C094
 Probe Info  		43.11  LDD1785  [4]
C134
 Probe Info  		20.82  LDD1816  [4]
C143
 Probe Info  		12.73  LDD1825  [4]
C201
 Probe Info  		26.17  LDD1877  [4]
C218
 Probe Info  		12.55  LDD1892  [4]
C220
 Probe Info  		15.67  LDD1894  [4]
C264
 Probe Info  		18.77  LDD1935  [4]
C349
 Probe Info  		10.63  LDD2010  [4]
C350
 Probe Info  		28.64  LDD2011  [4]
C362
 Probe Info  		26.35  LDD2023  [4]
C388
 Probe Info  		39.12  LDD2047  [4]
C407
 Probe Info  		12.13  LDD2064  [4]
STS-2
 Probe Info  		N.A.  LDD0138  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bifunctional polynucleotide phosphatase/kinase (PNKP) DNA 3' phosphatase family Q96T60
Probable glutathione peroxidase 8 (GPX8) Glutathione peroxidase family Q8TED1
Microsomal glutathione S-transferase 2 (MGST2) MAPEG family Q99735
GTPase IMAP family member 5 (GIMAP5) AIG1/Toc34/Toc159-like paraseptin GTPase family Q96F15
Transporter and channel
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Acid-sensing ion channel 1 (ASIC1) Amiloride-sensitive sodium channel (TC 1.A.6) family P78348
Bcl-2-like protein 2 (BCL2L2) Bcl-2 family Q92843
Gap junction alpha-8 protein (GJA8) Connexin family P48165
Cardiac phospholamban (PLN) Phospholamban family P26678
Vesicle-associated membrane protein 3 (VAMP3) Synaptobrevin family Q15836
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) BZIP family Q96BA8
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
C-C motif chemokine 3-like 1 (CCL3L1; CCL3L3) Intercrine beta (chemokine CC) family P16619
Other
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bcl-2-like protein 10 (BCL2L10) Bcl-2 family Q9HD36
Cortexin-3 (CTXN3) Cortexin family Q4LDR2
Protein kish-B (TMEM167B) KISH family Q9NRX6
Beta-sarcoglycan (SGCB) Sarcoglycan beta/delta/gamma/zeta family Q16585
Vesicle-trafficking protein SEC22a (SEC22A) Synaptobrevin family Q96IW7
Vesicle-trafficking protein SEC22b (SEC22B) Synaptobrevin family O75396
Syntaxin-8 (STX8) Syntaxin family Q9UNK0
C-type lectin domain family 14 member A (CLEC14A) . Q86T13
DnaJ homolog subfamily C member 30, mitochondrial (DNAJC30) . Q96LL9
TPA-induced transmembrane protein (TTMP) . Q5BVD1
Transmembrane protein 207 (TMEM207) . Q6UWW9

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Glutathione Small molecular drug DB00143

References

1 Fatty Acyl Sulfonyl Fluoride as an Activity-Based Probe for Profiling Fatty Acid-Associated Proteins in Living Cells. Chembiochem. 2022 Feb 16;23(4):e202100628. doi: 10.1002/cbic.202100628. Epub 2021 Dec 30.
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
5 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.