Probe Name	C122					Probe Info
Probe concentration	Probe:50uM; negative probe:50uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	TMT
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP12363	Lysophosphatidic acid phosphatase type 6 (ACP6)					15.5
LDTP03331	Proteasome subunit alpha type-2 (PSMA2)					8.4
LDTP02679	Prolyl 4-hydroxylase subunit alpha-1 (P4HA1)					7.7
LDTP06375	Delta(24)-sterol reductase (DHCR24)					7.1
LDTP05758	S-methyl-5'-thioadenosine phosphorylase (MTAP)					6.1
LDTP03525	Cell division cycle protein 27 homolog (CDC27)					5.5
LDTP04712	Puromycin-sensitive aminopeptidase (NPEPPS)					5.4
LDTP05476	3-ketodihydrosphingosine reductase (KDSR)					5.2

Target ID	Target Name					Binding Ratio
LDTP03277	ER lumen protein-retaining receptor 1 (KDELR1)					10.0
LDTP01059	Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B)					8.1
LDTP02724	Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2)					7.5
LDTP10913	Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A)					6.8
LDTP07885	Mitochondrial S-adenosylmethionine carrier protein (SLC25A26)					5.6
LDTP11813	Solute carrier family 25 member 32 (SLC25A32)					5.1

Target ID	Target Name					Binding Ratio
LDTP12565	Mitochondrial import receptor subunit TOM22 homolog (TOMM22)					30.9
LDTP03678	ER lumen protein-retaining receptor 2 (KDELR2)					10.1
LDTP05375	Nucleobindin-1 (NUCB1)					5.9
LDTP14486	Clustered mitochondria protein homolog (CLUH)					5.5

1	Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26. Mass spectrometry data entry: PXD041587