Details of the Target

General Information of Target

Target ID LDTP07583
Target Name Transmembrane protein 65 (TMEM65)
Gene Name TMEM65
Gene ID 157378
Synonyms
Transmembrane protein 65
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSRLLPLLRSRTARSLRPGPAAAAAPRPPSWCCCGRGLLALAPPGGLPGGPRRLGTHPKK
EPMEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEAPPPTPGQLRYVFIH
NAIPFIGFGFLDNAIMIVAGTHIEMSIGIILGISTMAAAALGNLVSDLAGLGLAGYVEAL
ASRLGLSIPDLTPKQVDMWQTRLSTHLGKAVGVTIGCILGMFPLIFFGGGEEDEKLETKS
Target Bioclass
Transporter and channel
Subcellular location
Cell membrane
Function
Essential for maintaining proper cardiac intercalated disk (ICD) structure and function as well as cardiac conduction velocity in the heart. Its association with SCN1B is required for stabilizing the perinexus in the ICD and for localization of GJA1 and SCN5A to the ICD. May regulate the function of the gap junction protein GJA1 and may contribute to the stability and proper localization of GJA1 to cardiac intercalated disk thereby regulating gap junction communication. May also play a role in the regulation of mitochondrial respiration and mitochondrial DNA copy number maintenance.
Uniprot ID
Q6PI78
Ensemble ID
ENST00000297632.8
HGNC ID
HGNC:25203

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Acrolein
 Probe Info  		N.A.  LDD0227  [1]
DBIA
 Probe Info  		C217(0.88)  LDD1507  [2]
AOyne
 Probe Info  		15.00  LDD0443  [3]
PAL-AfBPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C100
 Probe Info  		6.82  LDD1789  [4]
C169
 Probe Info  		25.63  LDD1849  [4]
C220
 Probe Info  		45.57  LDD1894  [4]
C228
 Probe Info  		25.28  LDD1901  [4]
C238
 Probe Info  		12.91  LDD1911  [4]
C246
 Probe Info  		13.09  LDD1919  [4]
C349
 Probe Info  		8.51  LDD2010  [4]
C350
 Probe Info  		25.46  LDD2011  [4]
C388
 Probe Info  		64.00  LDD2047  [4]
C390
 Probe Info  		48.17  LDD2049  [4]
C419
 Probe Info  		8.94  LDD2074  [4]
FFF probe13
 Probe Info  		20.00  LDD0475  [5]
JN0003
 Probe Info  		20.00  LDD0469  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C217(0.88)  LDD1507  [2]
 LDCM0215  AC10 HEK-293T C217(1.04)  LDD1508  [2]
 LDCM0237  AC12 HEK-293T C217(0.88)  LDD1510  [2]
 LDCM0276  AC17 HEK-293T C217(0.78)  LDD1515  [2]
 LDCM0277  AC18 HEK-293T C217(0.93)  LDD1516  [2]
 LDCM0279  AC2 HEK-293T C217(0.94)  LDD1518  [2]
 LDCM0280  AC20 HEK-293T C217(0.79)  LDD1519  [2]
 LDCM0285  AC25 HEK-293T C217(0.65)  LDD1524  [2]
 LDCM0286  AC26 HEK-293T C217(0.92)  LDD1525  [2]
 LDCM0288  AC28 HEK-293T C217(1.13)  LDD1527  [2]
 LDCM0294  AC33 HEK-293T C217(0.81)  LDD1533  [2]
 LDCM0295  AC34 HEK-293T C217(1.22)  LDD1534  [2]
 LDCM0297  AC36 HEK-293T C217(1.16)  LDD1536  [2]
 LDCM0301  AC4 HEK-293T C217(1.05)  LDD1540  [2]
 LDCM0303  AC41 HEK-293T C217(0.90)  LDD1542  [2]
 LDCM0304  AC42 HEK-293T C217(1.23)  LDD1543  [2]
 LDCM0306  AC44 HEK-293T C217(1.05)  LDD1545  [2]
 LDCM0311  AC49 HEK-293T C217(0.89)  LDD1550  [2]
 LDCM0313  AC50 HEK-293T C217(1.18)  LDD1552  [2]
 LDCM0315  AC52 HEK-293T C217(0.97)  LDD1554  [2]
 LDCM0320  AC57 HEK-293T C217(1.01)  LDD1559  [2]
 LDCM0321  AC58 HEK-293T C217(0.85)  LDD1560  [2]
 LDCM0324  AC60 HEK-293T C217(0.92)  LDD1563  [2]
 LDCM0356  AKOS034007680 HEK-293T C217(0.79)  LDD1570  [2]
 LDCM0371  CL102 HEK-293T C217(0.93)  LDD1575  [2]
 LDCM0375  CL106 HEK-293T C217(0.99)  LDD1579  [2]
 LDCM0380  CL110 HEK-293T C217(0.99)  LDD1584  [2]
 LDCM0384  CL114 HEK-293T C217(0.89)  LDD1588  [2]
 LDCM0388  CL118 HEK-293T C217(0.91)  LDD1592  [2]
 LDCM0393  CL122 HEK-293T C217(1.12)  LDD1597  [2]
 LDCM0397  CL126 HEK-293T C217(0.90)  LDD1601  [2]
 LDCM0401  CL14 HEK-293T C217(0.85)  LDD1605  [2]
 LDCM0404  CL17 HEK-293T C217(0.92)  LDD1608  [2]
 LDCM0405  CL18 HEK-293T C217(1.10)  LDD1609  [2]
 LDCM0407  CL2 HEK-293T C217(0.96)  LDD1611  [2]
 LDCM0408  CL20 HEK-293T C217(1.13)  LDD1612  [2]
 LDCM0414  CL26 HEK-293T C217(0.99)  LDD1618  [2]
 LDCM0417  CL29 HEK-293T C217(0.85)  LDD1621  [2]
 LDCM0419  CL30 HEK-293T C217(1.18)  LDD1623  [2]
 LDCM0421  CL32 HEK-293T C217(1.26)  LDD1625  [2]
 LDCM0431  CL41 HEK-293T C217(0.99)  LDD1635  [2]
 LDCM0432  CL42 HEK-293T C217(0.77)  LDD1636  [2]
 LDCM0434  CL44 HEK-293T C217(0.88)  LDD1638  [2]
 LDCM0440  CL5 HEK-293T C217(0.94)  LDD1644  [2]
 LDCM0441  CL50 HEK-293T C217(0.96)  LDD1645  [2]
 LDCM0444  CL53 HEK-293T C217(0.83)  LDD1647  [2]
 LDCM0445  CL54 HEK-293T C217(0.77)  LDD1648  [2]
 LDCM0447  CL56 HEK-293T C217(1.04)  LDD1650  [2]
 LDCM0451  CL6 HEK-293T C217(1.47)  LDD1654  [2]
 LDCM0454  CL62 HEK-293T C217(0.94)  LDD1657  [2]
 LDCM0457  CL65 HEK-293T C217(0.74)  LDD1660  [2]
 LDCM0458  CL66 HEK-293T C217(0.93)  LDD1661  [2]
 LDCM0460  CL68 HEK-293T C217(1.38)  LDD1663  [2]
 LDCM0467  CL74 HEK-293T C217(0.99)  LDD1670  [2]
 LDCM0470  CL77 HEK-293T C217(0.92)  LDD1673  [2]
 LDCM0471  CL78 HEK-293T C217(1.11)  LDD1674  [2]
 LDCM0473  CL8 HEK-293T C217(1.18)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C217(1.15)  LDD1677  [2]
 LDCM0480  CL86 HEK-293T C217(1.14)  LDD1683  [2]
 LDCM0483  CL89 HEK-293T C217(0.92)  LDD1686  [2]
 LDCM0485  CL90 HEK-293T C217(1.13)  LDD1688  [2]
 LDCM0487  CL92 HEK-293T C217(0.90)  LDD1690  [2]
 LDCM0493  CL98 HEK-293T C217(1.05)  LDD1696  [2]
 LDCM0427  Fragment51 HEK-293T C217(1.03)  LDD1631  [2]
 LDCM0109  NEM HeLa N.A.  LDD0227  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Phosphatidylserine lipase ABHD16A (ABHD16A) ABHD16 family O95870
3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP) EBP family Q15125
Prostaglandin E synthase (PTGES) MAPEG family O14684
Estradiol 17-beta-dehydrogenase 11 (HSD17B11) Short-chain dehydrogenases/reductases (SDR) family Q8NBQ5
ADP-ribosylation factor-like protein 13B (ARL13B) Arf family Q3SXY8
Methylsterol monooxygenase 1 (MSMO1) Sterol desaturase family Q15800
Plasma membrane ascorbate-dependent reductase CYBRD1 (CYBRD1) . Q53TN4
Transmembrane ascorbate-dependent reductase CYB561 (CYB561) . P49447
Transporter and channel
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Large neutral amino acids transporter small subunit 2 (SLC7A8) L-type amino acid transporter (LAT) (TC 2.A.3.8) family Q9UHI5
Sodium-dependent organic anion transporter (SLC10A6) Bile acid:sodium symporter (BASS) family Q3KNW5
Gap junction alpha-8 protein (GJA8) Connexin family P48165
Junctophilin-1 (JPH1) Junctophilin family Q9HDC5
Aquaporin-6 (AQP6) MIP/aquaporin (TC 1.A.8) family Q13520
Sodium channel regulatory subunit beta-3 (SCN3B) Sodium channel auxiliary subunit SCN3B family Q9NY72
Protrudin (ZFYVE27) . Q5T4F4
Thioredoxin-related transmembrane protein 2 (TMX2) . Q9Y320
Transmembrane protein 101 (TMEM101) . Q96IK0
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
G-protein coupled receptor 42 (GPR42) G-protein coupled receptor 1 family O15529
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Poliovirus receptor (PVR) Nectin family P15151
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Interleukin-3 receptor subunit alpha (IL3RA) Type I cytokine receptor family P26951
Other
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Receptor expression-enhancing protein 4 (REEP4) DP1 family Q9H6H4
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) ERGIC family Q9Y282
Membrane protein FAM174A (FAM174A) FAM174 family Q8TBP5
Protein FAM209A (FAM209A) FAM209 family Q5JX71
Protein jagunal homolog 1 (JAGN1) Jagunal family Q8N5M9
Reticulophagy regulator 3 (RETREG3) RETREG family Q86VR2
Complex I assembly factor TIMMDC1, mitochondrial (TIMMDC1) Tim17/Tim22/Tim23 family Q9NPL8
PDZK1-interacting protein 1 (PDZK1IP1) . Q13113
Transmembrane protein 80 (TMEM80) . Q96HE8

References

1 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
4 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
5 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.