Details of the Target

General Information of Target

Target ID LDTP01200
Target Name Peroxisomal membrane protein PEX14 (PEX14)
Gene Name PEX14
Gene ID 5195
Synonyms
Peroxisomal membrane protein PEX14; PTS1 receptor-docking protein; Peroxin-14; Peroxisomal membrane anchor protein PEX14
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASSEQAEQPSQPSSTPGSENVLPREPLIATAVKFLQNSRVRQSPLATRRAFLKKKGLTD
EEIDMAFQQSGTAADEPSSLGPATQVVPVQPPHLISQPYSPAGSRWRDYGALAIIMAGIA
FGFHQLYKKYLLPLILGGREDRKQLERMEAGLSELSGSVAQTVTQLQTTLASVQELLIQQ
QQKIQELAHELAAAKATTSTNWILESQNINELKSEINSLKGLLLNRRQFPPSPSAPKIPS
WQIPVKSPSPSSPAAVNHHSSSDISPVSNESTSSSPGKEGHSPEGSTVTYHLLGPQEEGE
GVVDVKGQVRMEVQGEEEKREDKEDEEDEEDDDVSHVDEEDCLGVQREDRRGGDGQINEQ
VEKLRRPEGASNESERD
Target Bioclass
Transporter and channel
Family
Peroxin-14 family
Subcellular location
Peroxisome membrane
Function
Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix. Plays a key role for peroxisome movement through a direct interaction with tubulin.
Uniprot ID
O75381
Ensemble ID
ENST00000356607.9
HGNC ID
HGNC:8856
ChEMBL ID
CHEMBL4523152

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
IM95 SNV: p.G103D .
JURKAT SNV: p.S252T .
KPL1 Substitution: p.S265L .
MCF7 Substitution: p.S265L .
MOLT4 SNV: p.S14N; p.A194V .
OCUG1 SNV: p.D332N .
RKO Deletion: p.V345CfsTer47 .
SF295 SNV: p.Q180K .
SKMEL2 SNV: p.T169A .
TOV21G SNV: p.S271Y .
UACC257 Substitution: p.S39F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
TG42
 Probe Info  		4.59  LDD0043  [2]
HPAP
 Probe Info  		3.01  LDD0063  [3]
Acrolein
 Probe Info  		N.A.  LDD0225  [4]
DBIA
 Probe Info  		C342(1.16)  LDD1492  [5]
5E-2FA
 Probe Info  		N.A.  LDD2235  [6]
IPM
 Probe Info  		N.A.  LDD2156  [7]
STPyne
 Probe Info  		N.A.  LDD0009  [8]
AOyne
 Probe Info  		15.00  LDD0443  [9]
PAL-AfBPP Probe
Click To Hide/Show 49 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C022
 Probe Info  		8.06  LDD1728  [10]
C027
 Probe Info  		6.06  LDD1733  [10]
C040
 Probe Info  		11.71  LDD1740  [10]
C056
 Probe Info  		18.77  LDD1753  [10]
C092
 Probe Info  		17.75  LDD1783  [10]
C094
 Probe Info  		29.45  LDD1785  [10]
C100
 Probe Info  		5.39  LDD1789  [10]
C106
 Probe Info  		18.00  LDD1793  [10]
C107
 Probe Info  		5.58  LDD1794  [10]
C112
 Probe Info  		25.99  LDD1799  [10]
C134
 Probe Info  		30.48  LDD1816  [10]
C143
 Probe Info  		27.28  LDD1825  [10]
C165
 Probe Info  		13.36  LDD1845  [10]
C166
 Probe Info  		7.67  LDD1846  [10]
C194
 Probe Info  		9.99  LDD1870  [10]
C196
 Probe Info  		11.96  LDD1872  [10]
C198
 Probe Info  		11.96  LDD1874  [10]
C201
 Probe Info  		31.34  LDD1877  [10]
C210
 Probe Info  		42.22  LDD1884  [10]
C218
 Probe Info  		28.05  LDD1892  [10]
C220
 Probe Info  		14.83  LDD1894  [10]
C228
 Probe Info  		16.11  LDD1901  [10]
C231
 Probe Info  		24.93  LDD1904  [10]
C232
 Probe Info  		73.01  LDD1905  [10]
C233
 Probe Info  		10.56  LDD1906  [10]
C235
 Probe Info  		20.97  LDD1908  [10]
C238
 Probe Info  		11.39  LDD1911  [10]
C246
 Probe Info  		17.51  LDD1919  [10]
C264
 Probe Info  		18.38  LDD1935  [10]
C270
 Probe Info  		12.13  LDD1940  [10]
C278
 Probe Info  		55.33  LDD1948  [10]
C282
 Probe Info  		15.03  LDD1952  [10]
C287
 Probe Info  		9.19  LDD1957  [10]
C289
 Probe Info  		62.25  LDD1959  [10]
C293
 Probe Info  		17.88  LDD1963  [10]
C296
 Probe Info  		20.11  LDD1966  [10]
C343
 Probe Info  		13.93  LDD2005  [10]
C349
 Probe Info  		8.75  LDD2010  [10]
C350
 Probe Info  		28.25  LDD2011  [10]
C355
 Probe Info  		23.10  LDD2016  [10]
C361
 Probe Info  		17.03  LDD2022  [10]
C362
 Probe Info  		32.00  LDD2023  [10]
C388
 Probe Info  		40.79  LDD2047  [10]
C390
 Probe Info  		21.11  LDD2049  [10]
C391
 Probe Info  		12.82  LDD2050  [10]
C407
 Probe Info  		18.13  LDD2064  [10]
C413
 Probe Info  		14.12  LDD2069  [10]
C426
 Probe Info  		13.45  LDD2081  [10]
C431
 Probe Info  		13.64  LDD2086  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C342(1.00)  LDD1508  [5]
 LDCM0237  AC12 HEK-293T C342(1.05)  LDD1510  [5]
 LDCM0259  AC14 HEK-293T C342(1.05)  LDD1512  [5]
 LDCM0270  AC15 HEK-293T C342(1.18)  LDD1513  [5]
 LDCM0277  AC18 HEK-293T C342(0.96)  LDD1516  [5]
 LDCM0279  AC2 HEK-293T C342(0.95)  LDD1518  [5]
 LDCM0280  AC20 HEK-293T C342(0.99)  LDD1519  [5]
 LDCM0282  AC22 HEK-293T C342(1.08)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C342(1.00)  LDD1522  [5]
 LDCM0284  AC24 HEK-293T C342(1.14)  LDD1523  [5]
 LDCM0286  AC26 HEK-293T C342(0.91)  LDD1525  [5]
 LDCM0288  AC28 HEK-293T C342(0.90)  LDD1527  [5]
 LDCM0291  AC30 HEK-293T C342(0.97)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C342(0.91)  LDD1531  [5]
 LDCM0293  AC32 HEK-293T C342(1.12)  LDD1532  [5]
 LDCM0295  AC34 HEK-293T C342(1.04)  LDD1534  [5]
 LDCM0297  AC36 HEK-293T C342(0.97)  LDD1536  [5]
 LDCM0299  AC38 HEK-293T C342(1.06)  LDD1538  [5]
 LDCM0300  AC39 HEK-293T C342(1.06)  LDD1539  [5]
 LDCM0301  AC4 HEK-293T C342(0.94)  LDD1540  [5]
 LDCM0302  AC40 HEK-293T C342(1.28)  LDD1541  [5]
 LDCM0304  AC42 HEK-293T C342(0.95)  LDD1543  [5]
 LDCM0306  AC44 HEK-293T C342(1.09)  LDD1545  [5]
 LDCM0308  AC46 HEK-293T C342(1.10)  LDD1547  [5]
 LDCM0309  AC47 HEK-293T C342(1.12)  LDD1548  [5]
 LDCM0310  AC48 HEK-293T C342(1.24)  LDD1549  [5]
 LDCM0313  AC50 HEK-293T C342(0.97)  LDD1552  [5]
 LDCM0315  AC52 HEK-293T C342(1.14)  LDD1554  [5]
 LDCM0317  AC54 HEK-293T C342(1.14)  LDD1556  [5]
 LDCM0318  AC55 HEK-293T C342(1.23)  LDD1557  [5]
 LDCM0319  AC56 HEK-293T C342(1.18)  LDD1558  [5]
 LDCM0321  AC58 HEK-293T C342(0.98)  LDD1560  [5]
 LDCM0323  AC6 HEK-293T C342(0.86)  LDD1562  [5]
 LDCM0324  AC60 HEK-293T C342(0.92)  LDD1563  [5]
 LDCM0326  AC62 HEK-293T C342(0.96)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C342(0.91)  LDD1566  [5]
 LDCM0328  AC64 HEK-293T C342(1.02)  LDD1567  [5]
 LDCM0334  AC7 HEK-293T C342(0.86)  LDD1568  [5]
 LDCM0345  AC8 HEK-293T C342(0.95)  LDD1569  [5]
 LDCM0275  AKOS034007705 HEK-293T C342(1.15)  LDD1514  [5]
 LDCM0156  Aniline NCI-H1299 15.00  LDD0403  [1]
 LDCM0368  CL10 HEK-293T C342(0.82)  LDD1572  [5]
 LDCM0369  CL100 HEK-293T C342(1.18)  LDD1573  [5]
 LDCM0371  CL102 HEK-293T C342(0.91)  LDD1575  [5]
 LDCM0372  CL103 HEK-293T C342(1.18)  LDD1576  [5]
 LDCM0373  CL104 HEK-293T C342(1.09)  LDD1577  [5]
 LDCM0375  CL106 HEK-293T C342(1.02)  LDD1579  [5]
 LDCM0376  CL107 HEK-293T C342(1.21)  LDD1580  [5]
 LDCM0377  CL108 HEK-293T C342(1.11)  LDD1581  [5]
 LDCM0379  CL11 HEK-293T C342(0.98)  LDD1583  [5]
 LDCM0380  CL110 HEK-293T C342(0.92)  LDD1584  [5]
 LDCM0381  CL111 HEK-293T C342(1.05)  LDD1585  [5]
 LDCM0382  CL112 HEK-293T C342(1.11)  LDD1586  [5]
 LDCM0384  CL114 HEK-293T C342(0.92)  LDD1588  [5]
 LDCM0385  CL115 HEK-293T C342(1.15)  LDD1589  [5]
 LDCM0386  CL116 HEK-293T C342(1.15)  LDD1590  [5]
 LDCM0388  CL118 HEK-293T C342(1.05)  LDD1592  [5]
 LDCM0389  CL119 HEK-293T C342(1.07)  LDD1593  [5]
 LDCM0390  CL12 HEK-293T C342(1.35)  LDD1594  [5]
 LDCM0391  CL120 HEK-293T C342(1.13)  LDD1595  [5]
 LDCM0393  CL122 HEK-293T C342(1.10)  LDD1597  [5]
 LDCM0394  CL123 HEK-293T C342(1.04)  LDD1598  [5]
 LDCM0395  CL124 HEK-293T C342(1.20)  LDD1599  [5]
 LDCM0397  CL126 HEK-293T C342(0.94)  LDD1601  [5]
 LDCM0398  CL127 HEK-293T C342(0.97)  LDD1602  [5]
 LDCM0399  CL128 HEK-293T C342(0.98)  LDD1603  [5]
 LDCM0401  CL14 HEK-293T C342(1.16)  LDD1605  [5]
 LDCM0402  CL15 HEK-293T C342(1.02)  LDD1606  [5]
 LDCM0403  CL16 HEK-293T C342(0.96)  LDD1607  [5]
 LDCM0405  CL18 HEK-293T C342(1.23)  LDD1609  [5]
 LDCM0407  CL2 HEK-293T C342(1.00)  LDD1611  [5]
 LDCM0408  CL20 HEK-293T C342(1.21)  LDD1612  [5]
 LDCM0410  CL22 HEK-293T C342(1.14)  LDD1614  [5]
 LDCM0411  CL23 HEK-293T C342(1.34)  LDD1615  [5]
 LDCM0412  CL24 HEK-293T C342(1.31)  LDD1616  [5]
 LDCM0414  CL26 HEK-293T C342(1.08)  LDD1618  [5]
 LDCM0415  CL27 HEK-293T C342(0.96)  LDD1619  [5]
 LDCM0416  CL28 HEK-293T C342(0.93)  LDD1620  [5]
 LDCM0418  CL3 HEK-293T C342(0.95)  LDD1622  [5]
 LDCM0419  CL30 HEK-293T C342(1.11)  LDD1623  [5]
 LDCM0421  CL32 HEK-293T C342(1.09)  LDD1625  [5]
 LDCM0423  CL34 HEK-293T C342(1.25)  LDD1627  [5]
 LDCM0424  CL35 HEK-293T C342(1.30)  LDD1628  [5]
 LDCM0425  CL36 HEK-293T C342(1.13)  LDD1629  [5]
 LDCM0428  CL39 HEK-293T C342(1.02)  LDD1632  [5]
 LDCM0429  CL4 HEK-293T C342(1.07)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C342(0.97)  LDD1634  [5]
 LDCM0432  CL42 HEK-293T C342(1.05)  LDD1636  [5]
 LDCM0434  CL44 HEK-293T C342(1.13)  LDD1638  [5]
 LDCM0436  CL46 HEK-293T C342(1.20)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C342(1.21)  LDD1641  [5]
 LDCM0438  CL48 HEK-293T C342(1.40)  LDD1642  [5]
 LDCM0441  CL50 HEK-293T C342(0.98)  LDD1645  [5]
 LDCM0443  CL52 HEK-293T C342(1.17)  LDD1646  [5]
 LDCM0445  CL54 HEK-293T C342(1.08)  LDD1648  [5]
 LDCM0447  CL56 HEK-293T C342(1.09)  LDD1650  [5]
 LDCM0449  CL58 HEK-293T C342(1.17)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C342(1.14)  LDD1653  [5]
 LDCM0451  CL6 HEK-293T C342(0.85)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C342(1.58)  LDD1655  [5]
 LDCM0454  CL62 HEK-293T C342(0.95)  LDD1657  [5]
 LDCM0455  CL63 HEK-293T C342(1.15)  LDD1658  [5]
 LDCM0456  CL64 HEK-293T C342(1.12)  LDD1659  [5]
 LDCM0458  CL66 HEK-293T C342(1.09)  LDD1661  [5]
 LDCM0460  CL68 HEK-293T C342(1.28)  LDD1663  [5]
 LDCM0463  CL70 HEK-293T C342(1.18)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C342(1.21)  LDD1667  [5]
 LDCM0465  CL72 HEK-293T C342(1.42)  LDD1668  [5]
 LDCM0467  CL74 HEK-293T C342(1.05)  LDD1670  [5]
 LDCM0469  CL76 HEK-293T C342(1.14)  LDD1672  [5]
 LDCM0471  CL78 HEK-293T C342(1.00)  LDD1674  [5]
 LDCM0473  CL8 HEK-293T C342(0.65)  LDD1676  [5]
 LDCM0474  CL80 HEK-293T C342(1.09)  LDD1677  [5]
 LDCM0476  CL82 HEK-293T C342(1.12)  LDD1679  [5]
 LDCM0477  CL83 HEK-293T C342(1.04)  LDD1680  [5]
 LDCM0478  CL84 HEK-293T C342(1.15)  LDD1681  [5]
 LDCM0480  CL86 HEK-293T C342(0.97)  LDD1683  [5]
 LDCM0481  CL87 HEK-293T C342(1.03)  LDD1684  [5]
 LDCM0482  CL88 HEK-293T C342(1.01)  LDD1685  [5]
 LDCM0485  CL90 HEK-293T C342(0.78)  LDD1688  [5]
 LDCM0487  CL92 HEK-293T C342(1.05)  LDD1690  [5]
 LDCM0489  CL94 HEK-293T C342(1.11)  LDD1692  [5]
 LDCM0490  CL95 HEK-293T C342(0.87)  LDD1693  [5]
 LDCM0491  CL96 HEK-293T C342(1.01)  LDD1694  [5]
 LDCM0493  CL98 HEK-293T C342(0.88)  LDD1696  [5]
 LDCM0494  CL99 HEK-293T C342(0.94)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C342(1.17)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C342(1.05)  LDD1671  [5]
 LDCM0427  Fragment51 HEK-293T C342(1.09)  LDD1631  [5]
 LDCM0022  KB02 HEK-293T C342(1.16)  LDD1492  [5]
 LDCM0023  KB03 HEK-293T C342(0.88)  LDD1497  [5]
 LDCM0024  KB05 HEK-293T C342(1.00)  LDD1502  [5]
 LDCM0109  NEM HeLa N.A.  LDD0225  [4]
 LDCM0014  Panhematin K562 3.01  LDD0063  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ATPase GET3 (GET3) ArsA ATPase family O43681
COP9 signalosome complex subunit 4 (COPS4) CSN4 family Q9BT78
Chromodomain-helicase-DNA-binding protein 5 (CHD5) SNF2/RAD54 helicase family Q8TDI0
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Peroxisomal biogenesis factor 19 (PEX19) Peroxin-19 family P40855
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Calmodulin-binding transcription activator 1 (CAMTA1) CAMTA family Q9Y6Y1
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Enhancer of filamentation 1 (NEDD9) CAS family Q14511
Centromere protein K (CENPK) CENP-K/MCM22 family Q9BS16
Cytoplasmic dynein 1 heavy chain 1 (DYNC1H1) Dynein heavy chain family Q14204
Protein FAM186B (FAM186B) FAM186 family Q8IYM0
Peroxisomal targeting signal 1 receptor (PEX5) Peroxisomal targeting signal receptor family P50542
Meiosis regulator and mRNA stability factor 1 (MARF1) . Q9Y4F3

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Design and synthesis of tailored human caseinolytic protease P inhibitors. Chem Commun (Camb). 2018 Aug 28;54(70):9833-9836. doi: 10.1039/c8cc05265d.
Mass spectrometry data entry: PXD010277
3 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587