Details of the Target

General Information of Target

Target ID LDTP07053
Target Name Cytochrome c oxidase assembly protein COX20, mitochondrial (COX20)
Gene Name COX20
Gene ID 116228
Synonyms
FAM36A; Cytochrome c oxidase assembly protein COX20, mitochondrial
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAPPEPGEPEERKSLKLLGFLDVENTPCARHSILYGSLGSVVAGFGHFLFTSRIRRSCD
VGVGGFILVTLGCWFHCRYNYAKQRIQERIAREEIKKKILYEGTHLDPERKHNGSSSN
Target Bioclass
Transporter and channel
Family
COX20 family
Subcellular location
Mitochondrion inner membrane
Function
Essential for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. Acts as a chaperone in the early steps of cytochrome c oxidase subunit II (MT-CO2/COX2) maturation, stabilizing the newly synthesized protein and presenting it to metallochaperones SCO1/2 which in turn facilitates the incorporation of the mature MT-CO2/COX2 into the assembling CIV holoenzyme.
Uniprot ID
Q5RI15
Ensemble ID
ENST00000366528.3
HGNC ID
HGNC:26970

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DEL Deletion: p.C77Ter .
DOTC24510 SNV: p.E109Q .
HEP3B217 SNV: p.R85I .
NUGC3 SNV: p.R89I DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
CHEMBL5175495
 Probe Info  		7.20  LDD0196  [1]
N1
 Probe Info  		100.00  LDD0242  [2]
STPyne
 Probe Info  		K17(5.88); K83(0.38); K98(10.00)  LDD0277  [3]
DBIA
 Probe Info  		C41(1.97)  LDD3311  [4]
JZ128-DTB
 Probe Info  		N.A.  LDD0462  [5]
IPM
 Probe Info  		C29(4.11)  LDD1701  [6]
m-APA
 Probe Info  		N.A.  LDD2231  [7]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [8]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [9]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [10]
TFBX
 Probe Info  		N.A.  LDD0027  [10]
WYneN
 Probe Info  		N.A.  LDD0021  [11]
Acrolein
 Probe Info  		N.A.  LDD0217  [12]
AOyne
 Probe Info  		15.00  LDD0443  [13]
NAIA_5
 Probe Info  		N.A.  LDD2223  [14]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C100
 Probe Info  		8.57  LDD1789  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C29(1.01)  LDD1508  [16]
 LDCM0237  AC12 HEK-293T C29(1.23)  LDD1510  [16]
 LDCM0277  AC18 HEK-293T C29(1.13)  LDD1516  [16]
 LDCM0279  AC2 HEK-293T C29(1.04)  LDD1518  [16]
 LDCM0280  AC20 HEK-293T C29(1.15)  LDD1519  [16]
 LDCM0281  AC21 HEK-293T C29(0.93)  LDD1520  [16]
 LDCM0284  AC24 HEK-293T C29(1.08)  LDD1523  [16]
 LDCM0286  AC26 HEK-293T C29(1.10)  LDD1525  [16]
 LDCM0288  AC28 HEK-293T C29(1.03)  LDD1527  [16]
 LDCM0289  AC29 HEK-293T C29(1.03)  LDD1528  [16]
 LDCM0293  AC32 HEK-293T C29(0.89)  LDD1532  [16]
 LDCM0295  AC34 HEK-293T C29(0.99)  LDD1534  [16]
 LDCM0297  AC36 HEK-293T C29(1.45)  LDD1536  [16]
 LDCM0298  AC37 HEK-293T C29(1.12)  LDD1537  [16]
 LDCM0301  AC4 HEK-293T C29(1.26)  LDD1540  [16]
 LDCM0302  AC40 HEK-293T C29(1.10)  LDD1541  [16]
 LDCM0304  AC42 HEK-293T C29(1.03)  LDD1543  [16]
 LDCM0306  AC44 HEK-293T C29(1.10)  LDD1545  [16]
 LDCM0307  AC45 HEK-293T C29(1.01)  LDD1546  [16]
 LDCM0310  AC48 HEK-293T C29(1.04)  LDD1549  [16]
 LDCM0312  AC5 HEK-293T C29(1.03)  LDD1551  [16]
 LDCM0313  AC50 HEK-293T C29(1.06)  LDD1552  [16]
 LDCM0315  AC52 HEK-293T C29(1.22)  LDD1554  [16]
 LDCM0316  AC53 HEK-293T C29(1.18)  LDD1555  [16]
 LDCM0319  AC56 HEK-293T C29(1.08)  LDD1558  [16]
 LDCM0321  AC58 HEK-293T C29(1.02)  LDD1560  [16]
 LDCM0324  AC60 HEK-293T C29(0.94)  LDD1563  [16]
 LDCM0325  AC61 HEK-293T C29(1.08)  LDD1564  [16]
 LDCM0328  AC64 HEK-293T C29(1.15)  LDD1567  [16]
 LDCM0345  AC8 HEK-293T C29(1.02)  LDD1569  [16]
 LDCM0248  AKOS034007472 HEK-293T C29(0.97)  LDD1511  [16]
 LDCM0275  AKOS034007705 HEK-293T C29(1.05)  LDD1514  [16]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [12]
 LDCM0372  CL103 HEK-293T C29(1.15)  LDD1576  [16]
 LDCM0376  CL107 HEK-293T C29(1.24)  LDD1580  [16]
 LDCM0381  CL111 HEK-293T C29(1.07)  LDD1585  [16]
 LDCM0385  CL115 HEK-293T C29(1.05)  LDD1589  [16]
 LDCM0389  CL119 HEK-293T C29(1.18)  LDD1593  [16]
 LDCM0390  CL12 HEK-293T C29(1.14)  LDD1594  [16]
 LDCM0394  CL123 HEK-293T C29(0.97)  LDD1598  [16]
 LDCM0398  CL127 HEK-293T C29(1.15)  LDD1602  [16]
 LDCM0402  CL15 HEK-293T C29(0.97)  LDD1606  [16]
 LDCM0405  CL18 HEK-293T C29(1.27)  LDD1609  [16]
 LDCM0408  CL20 HEK-293T C29(1.05)  LDD1612  [16]
 LDCM0409  CL21 HEK-293T C29(1.13)  LDD1613  [16]
 LDCM0412  CL24 HEK-293T C29(1.23)  LDD1616  [16]
 LDCM0415  CL27 HEK-293T C29(0.97)  LDD1619  [16]
 LDCM0418  CL3 HEK-293T C29(0.95)  LDD1622  [16]
 LDCM0419  CL30 HEK-293T C29(1.14)  LDD1623  [16]
 LDCM0421  CL32 HEK-293T C29(1.23)  LDD1625  [16]
 LDCM0422  CL33 HEK-293T C29(0.78)  LDD1626  [16]
 LDCM0425  CL36 HEK-293T C29(1.16)  LDD1629  [16]
 LDCM0428  CL39 HEK-293T C29(1.06)  LDD1632  [16]
 LDCM0432  CL42 HEK-293T C29(1.11)  LDD1636  [16]
 LDCM0434  CL44 HEK-293T C29(1.03)  LDD1638  [16]
 LDCM0435  CL45 HEK-293T C29(1.03)  LDD1639  [16]
 LDCM0438  CL48 HEK-293T C29(1.17)  LDD1642  [16]
 LDCM0445  CL54 HEK-293T C29(1.13)  LDD1648  [16]
 LDCM0447  CL56 HEK-293T C29(1.02)  LDD1650  [16]
 LDCM0448  CL57 HEK-293T C29(0.98)  LDD1651  [16]
 LDCM0451  CL6 HEK-293T C29(1.02)  LDD1654  [16]
 LDCM0452  CL60 HEK-293T C29(1.11)  LDD1655  [16]
 LDCM0455  CL63 HEK-293T C29(1.12)  LDD1658  [16]
 LDCM0458  CL66 HEK-293T C29(1.02)  LDD1661  [16]
 LDCM0460  CL68 HEK-293T C29(1.20)  LDD1663  [16]
 LDCM0461  CL69 HEK-293T C29(0.97)  LDD1664  [16]
 LDCM0465  CL72 HEK-293T C29(1.06)  LDD1668  [16]
 LDCM0471  CL78 HEK-293T C29(0.99)  LDD1674  [16]
 LDCM0473  CL8 HEK-293T C29(1.24)  LDD1676  [16]
 LDCM0474  CL80 HEK-293T C29(1.49)  LDD1677  [16]
 LDCM0475  CL81 HEK-293T C29(1.15)  LDD1678  [16]
 LDCM0478  CL84 HEK-293T C29(1.02)  LDD1681  [16]
 LDCM0481  CL87 HEK-293T C29(1.06)  LDD1684  [16]
 LDCM0484  CL9 HEK-293T C29(1.27)  LDD1687  [16]
 LDCM0485  CL90 HEK-293T C29(0.87)  LDD1688  [16]
 LDCM0487  CL92 HEK-293T C29(1.03)  LDD1690  [16]
 LDCM0488  CL93 HEK-293T C29(1.01)  LDD1691  [16]
 LDCM0491  CL96 HEK-293T C29(1.14)  LDD1694  [16]
 LDCM0494  CL99 HEK-293T C29(0.86)  LDD1697  [16]
 LDCM0495  E2913 HEK-293T C29(1.12)  LDD1698  [16]
 LDCM0625  F8 Ramos C29(0.57)  LDD2187  [17]
 LDCM0572  Fragment10 Ramos C29(1.17)  LDD2189  [17]
 LDCM0573  Fragment11 Ramos C29(0.02)  LDD2190  [17]
 LDCM0574  Fragment12 Ramos C29(2.00)  LDD2191  [17]
 LDCM0575  Fragment13 Ramos C29(0.92)  LDD2192  [17]
 LDCM0576  Fragment14 Ramos C29(2.50)  LDD2193  [17]
 LDCM0579  Fragment20 Ramos C29(2.12)  LDD2194  [17]
 LDCM0580  Fragment21 Ramos C29(1.07)  LDD2195  [17]
 LDCM0582  Fragment23 Ramos C29(1.66)  LDD2196  [17]
 LDCM0578  Fragment27 Ramos C29(0.84)  LDD2197  [17]
 LDCM0586  Fragment28 Ramos C29(0.55)  LDD2198  [17]
 LDCM0588  Fragment30 Ramos C29(0.85)  LDD2199  [17]
 LDCM0589  Fragment31 Ramos C29(1.29)  LDD2200  [17]
 LDCM0590  Fragment32 Ramos C29(1.30)  LDD2201  [17]
 LDCM0468  Fragment33 HEK-293T C29(1.13)  LDD1671  [16]
 LDCM0596  Fragment38 Ramos C29(1.13)  LDD2203  [17]
 LDCM0566  Fragment4 Ramos C29(1.49)  LDD2184  [17]
 LDCM0610  Fragment52 Ramos C29(0.82)  LDD2204  [17]
 LDCM0614  Fragment56 Ramos C29(0.81)  LDD2205  [17]
 LDCM0569  Fragment7 Ramos C29(1.15)  LDD2186  [17]
 LDCM0571  Fragment9 Ramos C29(2.63)  LDD2188  [17]
 LDCM0107  IAA HeLa N.A.  LDD0221  [12]
 LDCM0179  JZ128 PC-3 N.A.  LDD0462  [5]
 LDCM0022  KB02 Ramos C29(1.09)  LDD2182  [17]
 LDCM0023  KB03 MDA-MB-231 C29(4.11)  LDD1701  [6]
 LDCM0024  KB05 G361 C41(1.97)  LDD3311  [4]
 LDCM0109  NEM HeLa N.A.  LDD0223  [12]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amine oxidase [flavin-containing] B (MAOB) Flavin monoamine oxidase family P27338
Acidic mammalian chitinase (CHIA) Glycosyl hydrolase 18 family Q9BZP6
Vesicle transport protein GOT1A (GOLT1A) GOT1 family Q6ZVE7
Transporter and channel
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Hepatic sodium/bile acid cotransporter (SLC10A1) Bile acid:sodium symporter (BASS) family Q14973
Sodium-dependent organic anion transporter (SLC10A6) Bile acid:sodium symporter (BASS) family Q3KNW5
Gap junction alpha-8 protein (GJA8) Connexin family P48165
Membrane-associated progesterone receptor component 2 (PGRMC2) Cytochrome b5 family O15173
Novel acetylcholine receptor chaperone (TMEM35A) DoxX family Q53FP2
Aquaporin-6 (AQP6) MIP/aquaporin (TC 1.A.8) family Q13520
Guided entry of tail-anchored proteins factor 1 (GET1) WRB/GET1 family O00258
Thioredoxin-related transmembrane protein 2 (TMX2) . Q9Y320
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) BZIP family Q96BA8
Other
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) ERGIC family Q9Y282
FUN14 domain-containing protein 2 (FUNDC2) FUN14 family Q9BWH2
Protein jagunal homolog 1 (JAGN1) Jagunal family Q8N5M9
Transcription elongation factor A protein 2 (TCEA2) TFS-II family Q15560
Protein MENT (MENT) . Q9BUN1
Protein NCBP2AS2 (NCBP2AS2) . Q69YL0
Testis-expressed protein 44 (TEX44) . Q53QW1

References

1 Charting the Chemical Space of Acrylamide-Based Inhibitors of zDHHC20. ACS Med Chem Lett. 2022 Sep 26;13(10):1648-1654. doi: 10.1021/acsmedchemlett.2c00336. eCollection 2022 Oct 13.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
6 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
11 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
12 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
13 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
14 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
15 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
16 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
17 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578