Details of the Target

General Information of Target

Target ID LDTP04558
Target Name Arfaptin-1 (ARFIP1)
Gene Name ARFIP1
Gene ID 27236
Synonyms
Arfaptin-1; ADP-ribosylation factor-interacting protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAQESPKNSAAEIPVTSNGEVDDSREHSFNRDLKHSLPSGLGLSETQITSHGFDNTKEGV
IEAGAFQGSPAPPLPSVMSPSRVAASRLAQQGSDLIVPAGGQRTQTKSGPVILADEIKNP
AMEKLELVRKWSLNTYKCTRQIISEKLGRGSRTVDLELEAQIDILRDNKKKYENILKLAQ
TLSTQLFQMVHTQRQLGDAFADLSLKSLELHEEFGYNADTQKLLAKNGETLLGAINFFIA
SVNTLVNKTIEDTLMTVKQYESARIEYDAYRTDLEELNLGPRDANTLPKIEQSQHLFQAH
KEKYDKMRNDVSVKLKFLEENKVKVLHNQLVLFHNAIAAYFAGNQKQLEQTLKQFHIKLK
TPGVDAPSWLEEQ
Target Bioclass
Other
Subcellular location
Golgi apparatus
Function
Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells.
Uniprot ID
P53367
Ensemble ID
ENST00000353617.7
HGNC ID
HGNC:21496

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.E275G .
HCT15 SNV: p.R166M .
KMCH1 SNV: p.K324E .
NUGC3 SNV: p.A336T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
ONAyne
 Probe Info  		K146(1.43)  LDD0274  [2]
Jackson_1
 Probe Info  		4.31  LDD0120  [3]
Acrolein
 Probe Info  		N.A.  LDD0221  [4]
ATP probe
 Probe Info  		N.A.  LDD0199  [5]
NHS
 Probe Info  		N.A.  LDD0010  [6]
STPyne
 Probe Info  		N.A.  LDD0009  [6]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [7]
AOyne
 Probe Info  		15.00  LDD0443  [8]
PAL-AfBPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C027
 Probe Info  		5.31  LDD1733  [9]
C094
 Probe Info  		46.53  LDD1785  [9]
C235
 Probe Info  		26.54  LDD1908  [9]
C258
 Probe Info  		5.50  LDD1931  [9]
C299
 Probe Info  		13.83  LDD1968  [9]
C320
 Probe Info  		6.54  LDD1986  [9]
C361
 Probe Info  		23.43  LDD2022  [9]
C383
 Probe Info  		18.13  LDD2042  [9]
C391
 Probe Info  		14.93  LDD2050  [9]
FFF probe2
 Probe Info  		6.22  LDD0463  [10]
FFF probe3
 Probe Info  		20.00  LDD0464  [10]
Alk-rapa
 Probe Info  		11.51  LDD0213  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.40  LDD0403  [1]
 LDCM0070  Cisar_cp37 MDA-MB-231 4.31  LDD0120  [3]
 LDCM0191  Compound 21 HEK-293T 12.27  LDD0508  [10]
 LDCM0192  Compound 35 HEK-293T 6.17  LDD0509  [10]
 LDCM0193  Compound 36 HEK-293T 9.43  LDD0511  [10]
 LDCM0107  IAA HeLa N.A.  LDD0221  [4]
 LDCM0109  NEM HeLa N.A.  LDD0223  [4]
 LDCM0090  Rapamycin JHH-7 11.51  LDD0213  [11]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Deubiquitinase DESI2 (DESI2) DeSI family Q9BSY9
Sphingomyelin phosphodiesterase 2 (SMPD2) Neutral sphingomyelinase family O60906
Serine/threonine-protein kinase D1 (PRKD1) CAMK Ser/Thr protein kinase family Q15139
ADP-ribosylation factor 1 (ARF1) Arf family P84077
ADP-ribosylation factor-like protein 1 (ARL1) Arf family P40616
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Secretory carrier-associated membrane protein 1 (SCAMP1) SCAMP family O15126
Barttin (BSND) . Q8WZ55
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Tumor necrosis factor receptor superfamily member 10D (TNFRSF10D) . Q9UBN6
Other
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Keratin, type I cuticular Ha3-II (KRT33B) Intermediate filament family Q14525
Keratin, type I cytoskeletal 27 (KRT27) Intermediate filament family Q7Z3Y8
Protein reprimo (RPRM) Reprimo family Q9NS64
Vesicle transport protein SFT2A (SFT2D1) SFT2 family Q8WV19
Synaptogyrin-3 (SYNGR3) Synaptogyrin family O43761
Arfaptin-2 (ARFIP2) . P53365
Melanoma-associated antigen 6 (MAGEA6) . P43360
Small integral membrane protein 5 (SMIM5) . Q71RC9

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
10 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
11 Rapamycin targets STAT3 and impacts c-Myc to suppress tumor growth. Cell Chem Biol. 2022 Mar 17;29(3):373-385.e6. doi: 10.1016/j.chembiol.2021.10.006. Epub 2021 Oct 26.