Details of the Target

General Information of Target

Target ID LDTP10471
Target Name Protein disulfide-isomerase TMX3 (TMX3)
Gene Name TMX3
Gene ID 54495
Synonyms
KIAA1830; TXNDC10; Protein disulfide-isomerase TMX3; EC 5.3.4.1; Thioredoxin domain-containing protein 10; Thioredoxin-related transmembrane protein 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSPGGKFDFDDGGCYVGGWEAGRAHGYGVCTGPGAQGEYSGCWAHGFESLGVFTGPGGHS
YQGHWQQGKREGLGVERKSRWTYRGEWLGGLKGRSGVWESVSGLRYAGLWKDGFQDGYGT
ETYSDGGTYQGQWQAGKRHGYGVRQSVPYHQAALLRSPRRTSLDSGHSDPPTPPPPLPLP
GDEGGSPASGSRGGFVLAGPGDADGASSRKRTPAAGGFFRRSLLLSGLRAGGRRSSLGSK
RGSLRSEVSSEVGSTGPPGSEASGPPAAAPPALIEGSATEVYAGEWRADRRSGFGVSQRS
NGLRYEGEWLGNRRHGYGRTTRPDGSREEGKYKRNRLVHGGRVRSLLPLALRRGKVKEKV
DRAVEGARRAVSAARQRQEIAAARAADALLKAVAASSVAEKAVEAARMAKLIAQDLQPML
EAPGRRPRQDSEGSDTEPLDEDSPGVYENGLTPSEGSPELPSSPASSRQPWRPPACRSPL
PPGGDQGPFSSPKAWPEEWGGAGAQAEELAGYEAEDEAGMQGPGPRDGSPLLGGCSDSSG
SLREEEGEDEEPLPPLRAPAGTEPEPIAMLVLRGSSSRGPDAGCLTEELGEPAATERPAQ
PGAANPLVVGAVALLDLSLAFLFSQLLT
Target Bioclass
Enzyme
Family
Protein disulfide isomerase family
Subcellular location
Endoplasmic reticulum membrane
Function
Probable disulfide isomerase, which participates in the folding of proteins containing disulfide bonds. May act as a dithiol oxidase. Acts as a regulator of endoplasmic reticulum-mitochondria contact sites via its ability to regulate redox signals.
Uniprot ID
Q96JJ7
Ensemble ID
ENST00000299608.7
HGNC ID
HGNC:24718

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.I122S .
JURKAT SNV: p.R278S .
NALM6 SNV: p.Y49S .
NCIH1975 SNV: p.M290V .
NCIH2286 SNV: p.D362N
Substitution: p.F361I		 .
SH4 SNV: p.V18L .
SW1271 SNV: p.D284Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
TH211
 Probe Info  		Y99(12.61)  LDD0260  [2]
STPyne
 Probe Info  		K106(3.94); K119(9.32); K267(6.78); K58(8.07)  LDD0277  [3]
OPA-S-S-alkyne
 Probe Info  		K164(1.61); K119(3.06)  LDD3494  [4]
IA-alkyne
 Probe Info  		C53(1.29); C56(1.29)  LDD0304  [5]
HPAP
 Probe Info  		3.15  LDD0064  [6]
Alkyne-RA190
 Probe Info  		2.22  LDD0299  [5]
HHS-475
 Probe Info  		Y99(0.86)  LDD0264  [7]
HHS-465
 Probe Info  		Y99(10.00)  LDD2237  [8]
Acrolein
 Probe Info  		N.A.  LDD0227  [9]
NHS
 Probe Info  		N.A.  LDD0010  [10]
Ox-W18
 Probe Info  		N.A.  LDD2175  [11]
AOyne
 Probe Info  		15.00  LDD0443  [12]
PAL-AfBPP Probe
Click To Hide/Show 39 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C027
 Probe Info  		5.70  LDD1733  [13]
C040
 Probe Info  		9.19  LDD1740  [13]
C056
 Probe Info  		16.68  LDD1753  [13]
C063
 Probe Info  		11.24  LDD1760  [13]
C091
 Probe Info  		17.03  LDD1782  [13]
C092
 Probe Info  		37.79  LDD1783  [13]
C094
 Probe Info  		40.79  LDD1785  [13]
C106
 Probe Info  		18.51  LDD1793  [13]
C112
 Probe Info  		21.71  LDD1799  [13]
C134
 Probe Info  		25.46  LDD1816  [13]
C187
 Probe Info  		17.15  LDD1865  [13]
C201
 Probe Info  		36.00  LDD1877  [13]
C206
 Probe Info  		32.45  LDD1881  [13]
C210
 Probe Info  		46.53  LDD1884  [13]
C218
 Probe Info  		14.93  LDD1892  [13]
C220
 Probe Info  		12.82  LDD1894  [13]
C228
 Probe Info  		14.93  LDD1901  [13]
C231
 Probe Info  		18.64  LDD1904  [13]
C232
 Probe Info  		39.95  LDD1905  [13]
C233
 Probe Info  		10.85  LDD1906  [13]
C235
 Probe Info  		24.42  LDD1908  [13]
C285
 Probe Info  		20.11  LDD1955  [13]
C287
 Probe Info  		9.06  LDD1957  [13]
C289
 Probe Info  		45.89  LDD1959  [13]
C293
 Probe Info  		22.01  LDD1963  [13]
C299
 Probe Info  		6.63  LDD1968  [13]
C310
 Probe Info  		8.57  LDD1977  [13]
C314
 Probe Info  		11.47  LDD1981  [13]
C343
 Probe Info  		11.88  LDD2005  [13]
C349
 Probe Info  		15.14  LDD2010  [13]
C350
 Probe Info  		36.00  LDD2011  [13]
C362
 Probe Info  		30.91  LDD2023  [13]
C388
 Probe Info  		39.67  LDD2047  [13]
C407
 Probe Info  		10.85  LDD2064  [13]
FFF probe11
 Probe Info  		20.00  LDD0471  [14]
FFF probe13
 Probe Info  		20.00  LDD0475  [14]
STS-1
 Probe Info  		N.A.  LDD0137  [15]
VE-P
 Probe Info  		N.A.  LDD0396  [16]
OEA-DA
 Probe Info  		17.45  LDD0046  [17]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.50  LDD0403  [1]
 LDCM0116  HHS-0101 DM93 Y99(0.86)  LDD0264  [7]
 LDCM0117  HHS-0201 DM93 Y99(0.83)  LDD0265  [7]
 LDCM0118  HHS-0301 DM93 Y99(0.58)  LDD0266  [7]
 LDCM0119  HHS-0401 DM93 Y99(0.78)  LDD0267  [7]
 LDCM0120  HHS-0701 DM93 Y99(0.52)  LDD0268  [7]
 LDCM0109  NEM HeLa N.A.  LDD0227  [9]
 LDCM0014  Panhematin hPBMC 3.15  LDD0064  [6]
 LDCM0131  RA190 MM1.R 2.22  LDD0299  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial (DLST) 2-oxoacid dehydrogenase family P36957
Serine/threonine-protein kinase Nek7 (NEK7) NEK Ser/Thr protein kinase family Q8TDX7
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Calreticulin (CALR) Calreticulin family P27797
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Platelet endothelial cell adhesion molecule (PECAM1) . P16284
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Four and a half LIM domains protein 3 (FHL3) . Q13643

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
5 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
6 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
7 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
8 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
9 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
12 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
13 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
14 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
15 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
16 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
17 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570