Details of the Target

General Information of Target

Target ID LDTP11762
Target Name DNA-directed RNA polymerase III subunit RPC6 (POLR3F)
Gene Name POLR3F
Gene ID 10621
Synonyms
DNA-directed RNA polymerase III subunit RPC6; RNA polymerase III subunit C6; DNA-directed RNA polymerase III subunit F; RNA polymerase III 39 kDa subunit; RPC39
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGPLQGDGGPALGGADVAPRLSPVRVWPRPQAPKEPALHPMGLSLPKEKGLILCLWSKFC
RWFQRRESWAQSRDEQNLLQQKRIWESPLLLAAKDNDVQALNKLLKYEDCKVHQRGAMGE
TALHIAALYDNLEAAMVLMEAAPELVFEPMTSELYEGQTALHIAVVNQNMNLVRALLARR
ASVSARATGTAFRRSPCNLIYFGEHPLSFAACVNSEEIVRLLIEHGADIRAQDSLGNTVL
HILILQPNKTFACQMYNLLLSYDRHGDHLQPLDLVPNHQGLTPFKLAGVEGNTVMFQHLM
QKRKHTQWTYGPLTSTLYDLTEIDSSGDEQSLLELIITTKKREARQILDQTPVKELVSLK
WKRYGRPYFCMLGAIYLLYIICFTMCCIYRPLKPRTNNRTSPRDNTLLQQKLLQEAYMTP
KDDIRLVGELVTVIGAIIILLVEVPDIFRMGVTRFFGQTILGGPFHVLIITYAFMVLVTM
VMRLISASGEVVPMSFALVLGWCNVMYFARGFQMLGPFTIMIQKMIFGDLMRFCWLMAVV
ILGFASAFYIIFQTEDPEELGHFYDYPMALFSTFELFLTIIDGPANYNVDLPFMYSITYA
AFAIIATLLMLNLLIAMMGDTHWRVAHERDELWRAQIVATTVMLERKLPRCLWPRSGICG
REYGLGDRWFLRVEDRQDLNRQRIQRYAQAFHTRGSEDLDKDSVEKLELGCPFSPHLSLP
MPSVSRSTSRSSANWERLRQGTLRRDLRGIINRGLEDGESWEYQI
Target Bioclass
Enzyme
Family
Eukaryotic RPC34/RPC39 RNA polymerase subunit family
Subcellular location
Nucleus
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Part of POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer that coordinates the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses, including varicella zoster virus. Acts as a nuclear and cytosolic DNA sensor detecting AT-rich DNA, involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway. Preferentially binds double-stranded DNA (dsDNA).
Uniprot ID
Q9H1D9
Ensemble ID
ENST00000377603.5
HGNC ID
HGNC:15763

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C221(2.75)  LDD3312  [1]
Acrolein
 Probe Info  		N.A.  LDD0222  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [3]
NAIA_5
 Probe Info  		N.A.  LDD2223  [4]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C027
 Probe Info  		8.75  LDD1733  [5]
C314
 Probe Info  		15.35  LDD1981  [5]
VE-P
 Probe Info  		N.A.  LDD0396  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C221(1.19)  LDD1510  [7]
 LDCM0280  AC20 HEK-293T C221(0.95)  LDD1519  [7]
 LDCM0288  AC28 HEK-293T C221(1.04)  LDD1527  [7]
 LDCM0297  AC36 HEK-293T C221(1.26)  LDD1536  [7]
 LDCM0301  AC4 HEK-293T C221(1.20)  LDD1540  [7]
 LDCM0306  AC44 HEK-293T C221(1.07)  LDD1545  [7]
 LDCM0315  AC52 HEK-293T C221(1.06)  LDD1554  [7]
 LDCM0324  AC60 HEK-293T C221(1.09)  LDD1563  [7]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [2]
 LDCM0408  CL20 HEK-293T C221(1.40)  LDD1612  [7]
 LDCM0421  CL32 HEK-293T C221(1.10)  LDD1625  [7]
 LDCM0434  CL44 HEK-293T C221(1.05)  LDD1638  [7]
 LDCM0447  CL56 HEK-293T C221(1.27)  LDD1650  [7]
 LDCM0460  CL68 HEK-293T C221(1.20)  LDD1663  [7]
 LDCM0473  CL8 HEK-293T C221(1.58)  LDD1676  [7]
 LDCM0474  CL80 HEK-293T C221(1.17)  LDD1677  [7]
 LDCM0487  CL92 HEK-293T C221(1.32)  LDD1690  [7]
 LDCM0022  KB02 HEK-293T C26(0.91)  LDD1492  [7]
 LDCM0023  KB03 HEK-293T C26(1.00)  LDD1497  [7]
 LDCM0024  KB05 HMCB C221(2.75)  LDD3312  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA-directed RNA polymerase III subunit RPC3 (POLR3C) Eukaryotic RPC3/POLR3C RNA polymerase subunit family Q9BUI4
DNA-directed RNA polymerase III subunit RPC7 (POLR3G) Eukaryotic RPC7 RNA polymerase subunit family O15318
DNA-directed RNA polymerase III subunit RPC7-like (POLR3GL) Eukaryotic RPC7 RNA polymerase subunit family Q9BT43
Serine/threonine-protein kinase PRP4 homolog (PRPF4B) CMGC Ser/Thr protein kinase family Q13523
Ribose-phosphate pyrophosphokinase 1 (PRPS1) Ribose-phosphate pyrophosphokinase family P60891
Eukaryotic translation initiation factor 2 subunit 3 (EIF2S3) Classic translation factor GTPase family P41091
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Wolframin (WFS1) . O76024
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) ATP12 family Q8N5M1
Progranulin (GRN) Granulin family P28799
Heat shock-related 70 kDa protein 2 (HSPA2) Heat shock protein 70 family P54652
Proline-rich nuclear receptor coactivator 1 (PNRC1) PNRC family Q12796
Heat shock protein beta-1 (HSPB1) Small heat shock protein (HSP20) family P04792

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
6 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402